Dependence on Mincle and Dectin-2 Varies With Multiple Candida Species During Systemic Infection

FUNDING SO was supported by a Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society (Grant No. 099953/Z/12/Z) and by a Wellcome Trust Cross-Disciplinary Award. NG acknowledges Wellcome Trust support of a Senior Investigator (101873/Z/13/Z), Collaborative (200208/A/15/Z) and Strategic Awards (097377/Z11/Z) and the MRC Centre for Medical Mycology (MR/N006364/2). PT was funded by a Wellcome Trust Investigator Award (107964/Z/15/Z) and the UK Dementia Research Institute. ACKNOWLEDGMENTS We wish to acknowledge the NIH-sponsored Mutant Mouse Regional Resource Center (MMRRC) National System as the source of genetically altered mice (C57BL/6-Clec4et m1. 1C fg /Mmucd 031936-UCD) for use in this study. The mice were produced and deposited to the MMRRC by the Consortium for Functional Glycomics supported by the National Institute of General Medical Sciences (GM62116). We also thank the Microscopy and Histology Core Facility at the University of Aberdeen for expert assistance with TEM.Peer reviewedPublisher PD

Number of marandugrass tiller in consortium with corn and eucalyptus

Todos os textos, informa??es e resultados apresentados s?o de inteira responsabilidade dos autores.Objetivou-se avaliar o n?mero de perfilhos do capim-marandu em cons?rcio com milho, cultivado em diferentes arranjos de eucalipto em sistemas agrossilvipastoris, estabelecido h? cinco anos. O experimento foi conduzido na Universidade Federal de Vi?osa, MG, em um sistema agrossilvipastoril no in?cio de degrada??o do pasto. Utilizou-se o delineamento experimental em blocos completos casualizados, com tr?s repeti??es e os tratamentos consistiram do arranjo fatorial (3x2)+1, sendo fator A: cultivo de brachiaria brizantha cv. Marandu (capim-marandu) com dois h?bridos de milho (BM207, com folhas planiformes e BM502, com folhas eretas) e o capim-marandu em sistema silvipastoril e o fator B os dois espa?amentos entre ?rvores de eucalipto (12x2 e 12x4 m), e a testemunha em pleno sol (monocultivo). O n?mero de perfilhos foi estimada a partir da contagem total de perfilhos no interior de uma moldura de vergalh?o de 1,00 m de lado, alocada no interior da parcela na quando o milho apresentava gr?os pastoso farin?ceo (?poca de colheita de milho para ensilagem). O n?mero de perfilhos da Brachiaria brizantha cv. Marandu ? maior em sistemas que combinam h?bridos de milho de arquitetura foliar ereta, espa?amentos entre ?rvores maiores (12x4) e para os sistemas silvipastoris.It was aimed to assess the number of marandugrass tillers in consortium with corn grown in different eucalyptus arrangements in agroforestry systems, from five years ago. The experiment was conducted at the Universidade Federal de Vi?osa,in Minas Gerais, in an agrosylvopastoral system at the beginning of pasture degradation. We used the experimental design in randomized complete block, with three replications and the treatments consisted of a factorial arrangement (3x2)+1, factor A being: cultivation of Brachiariabrizantha cv. Marandu (marandugrass) with two corn hybrids (BM207, with flat leaves and BM502, with erect leaves) and marandugrass in silvopastoral system and factor B: two spacing between eucalyptus trees (12x2 and 12x4 meters), and the witness in full sun (monoculture). The number of tillers was estimated from the total count of tillers inside a framerebar of 1,00 meterwide, allocated inside the parcel when the corn had pasty chalky grains (time of harvest corn for silage). The number of Brachiariabrizantha tillers cv. Marandu is higher in systems that combine corn hybrids of erect leaf architecture, spacings between larger trees (12x4) and for silvopastoral systems

Morphological composition of marandugrass in agroforestry systems

Todos os textos, informa??es e resultados apresentados s?o de inteira responsabilidade dos autores.Objetivou-se avaliar a composi??o morfol?gica do capim-marandu no estabelecimento de pastos de capimmarandu ap?s renova??o de sistemas agrossilvipastoris com eucalipto. O experimento foi conduzido na Universidade Federal de Vi?osa, MG, em um sistema agrossilvipastoril no in?cio de degrada??o do pasto. Utilizou-se o delineamento experimental em blocos completos casualizados, com tr?s repeti??es e os tratamentos consistiram do arranjo fatorial (3x2)+1, sendo fator A: cultivo de brachiaria brizantha cv. Marandu (capimmarandu) com dois h?bridos de milho (BM207, com folhas planiformes e BM502, com folhas eretas) e o capimmarandu em sistema silvipastoril e o fator B os dois espa?amentos entre ?rvores de eucalipto (12x2 e 12x4 m), e a testemunha em pleno sol (monocultivo). As avalia??es foram aos 90 e 150 dias ap?s colheita do milho, determinando a massa seca das l?minas foliares verdes (%LFV) e colmos verdes (%CV). A composi??o morfol?gica da Brachiaria brizantha cv. Marandu ? alterada nos sistemas que combinam h?bridos de milho de arquitetura foliar ereta e espa?amentos entre ?rvores maiores (12x4), com maior porcentagem de laminas foliares. Os sistemas agrossilvipastoris s?o uma alternativa potencial a recupera??o de sistemas degradados, melhorando a composi??o morfol?gica do pasto.ABSTRACT It was aimed to evaluate the morphological composition of marandugrass in establishing marandugrass pastures after renovation of agroforestry systems with eucalyptus. The experiment was conducted at the Federal University of Vi?osa, in Minas Gerais, in an agrosylvopastoral system at the beginning of pasture degradation. We used the experimental design in randomized complete block, with three replications and the treatments consisted of a factorial arrangement (3x2)+1, factor A being: cultivation of Brachiaria brizantha cv. Marandu (marandugrass) with two corn hybrids (BM207, with flat leaves and BM502, with erect leaves) and marandugrass in silvopastoral system and factor B: two spacing between eucalyptus trees (12x2 and 12x4 meters), and the witness in full sun (monoculture).The evaluations were at 90 and 150 days after corn harvest, determining the dry mass of green leaf blades (% GLB) and green culms (% GC). Morphological composition of Brachiaria brizantha cv. Marandu changes in systems that combine corn hybrids with erect leaf architecture and spacings between larger trees (12x4) with highest percentage of leaf blades. The agroforestry systems are a potential alternative for recovering degraded systems, improving the morphological composition of the pasture

Marandugrass production after renovation of agroforestry system

Todos os textos, informa??es e resultados apresentados s?o de inteira responsabilidade dos autores.Objetivou-se avaliar o estabelecimento da pastagem a partir da produtividade do capim-marandu cultivado com diferentes h?bridos de milho e em arranjos de eucalipto em sistemas agrossilvipastoris, silvipastoris e em pleno sol, em sistemas agrossilvipastoril estabelecido h? cinco anos. Utilizou-se o delineamento experimental em blocos completos casualizados, com tr?s repeti??es e os tratamentos consistiram do arranjo fatorial (3x2)+1, sendo fator A: cultivo de brachiaria brizantha cv. Marandu (capim-marandu) com dois h?bridos de milho (BM207, com folhas planiformes e BM502, com folhas eretas) e o capim-marandu em sistema silvipastoril e o fator B os dois espa?amentos entre ?rvores de eucalipto (12x2 e 12x4 m), e a testemunha em pleno sol (monocultivo). A produ??o de capim-marandu (MS) foi estimada aos 90 e 150 dias ap?s colheita do milho. Houve maior produ??o de MS do capim-marandu nos arranjos de maior espa?amento arb?reo. A recupera??o do pasto por meio de sistemas agrossilvipastoris ? uma alternativa na recupera??o de sistemas degradados, haja vista, seu potencial produtivo.It was aimed to evaluate the pasture establishment from the productivity of marandugrass grown with different corn hybrids and in eucalyptus arrangements in agroforestry, silvopastoral and in full sun systems, in an agrosylvopastoral system from five years ago. We used the experimental design in randomized complete block, with three replications and the treatments consisted of a factorial arrangement (3x2)+1, factor A being: cultivation of Brachiaria brizantha cv. Marandu (marandugrass) with two corn hybrids (BM207, with flat leaves and BM502, with erect leaves) and marandugrass in silvopastoral system and factor B: two spacing between eucalyptus trees (12x2 and 12x4 meters), and the witness in full sun (monoculture). The marandugrass production (DM) was estimated at 90 and 150 days after corn harvest. There was a higher DM production of marandugrass in the arrangements of most tree spacing. The pasture recovery through agroforestry systems is an alternative in the recovery of degraded systems, given their productive potential

Growth characteristics of marandugrass tillers in consortium with corn and eucalyptus

Todos os textos, informa??es e resultados apresentados s?o de inteira responsabilidade dos autor.Objetivou-se avaliar as caracter?sticas de crescimento a partir da altura e comprimento de perfilho estendido do Brachiaria brizantha cv. Marandu (capim-marandu) em cons?rcio com milho, cultivado em diferentes arranjos de eucalipto em sistemas agrossilvipastoris, estabelecido h? cinco anos. O experimento foi conduzido na Universidade Federal de Vi?osa, MG, em um sistema agrossilvipastoril no in?cio de degrada??o do pasto. Utilizou-se o delineamento experimental em blocos completos casualizados, com tr?s repeti??es e os tratamentos consistiram do arranjo fatorial (3x2)+1, sendo fator A: cultivo de brachiaria brizantha cv. Marandu (capim-marandu) com dois h?bridos de milho (BM207, com folhas planiformes e BM502, com folhas eretas) e o capim-marandu em sistema silvipastoril e o fator B os dois espa?amentos entre ?rvores de eucalipto (12x2 e 12x4 m), e a testemunha em pleno sol (monocultivo). Aos 110 e 145 DAS tanto nos sistemas agrossilvipastoris, silvipastoris e em pleno sol, a altura de planta e comprimento de perfilho estendido foi determinada em ?rea delimitada com arma??o met?lica de um metro de lado em cada unidade experimental. A altura e comprimento de perfilho estendido da Brachiaria brizantha cv. Marandu ? maior nos sistemas silvipastoris e pleno sol e nos sistemas que combinam h?bridos de milho de arquitetura foliar planiforme (BM207), espa?amentos entre ?rvores maiores (12x4).ABSTRACT It was aimed to evaluate the growth characteristics from the height and length of extended tiller of Brachiariabrizantha cv. Marandu(marandugrass) in consortium with corn, grown in different eucalyptus arrangements in agroforestry systems, from five years ago. The experiment was conducted at the Universidade Federal de Vi?osa, in Minas Gerais, in an agrosylvopastoral system at the beginning of pasture degradation. We used the experimental design in randomized complete block, with three replications and the treatments consisted of a factorial arrangement (3x2) +1, factor A being: cultivation of Brachiariabrizantha cv. Marandu (marandugrass) with two corn hybrids (BM207, with flat leaves and BM502, with erect leaves) and marandugrass in silvopastoral system and factor B: two spacing between eucalyptus trees (12x2 and 12x4 meters), and the witness in full sun (monoculture). At 110 and 145 DAS in the systems agroforestry, silvipastoral and in full sun, plant height and length of extended tiller was determined in an enclosed area with metal frame of one meter wide in each experimental unit. The height and length of extended tiller of Brachiariabrizantha cv. Marandu is higher in silvopastoral and full sun systems and in systems that combine corn hybrids with flat leaf architecture (BM207), spacings between larger trees (12x4)

role of female sex hormone receptors

Funding Information: Funding: This study was supported by grant IECT-FAPEMA-05796/18 and FAPEMA IECT 30/2018-IECT Saúde, by the Research Center of the Portuguese Oncology Institute of Porto (project no. PI86-CI-IPOP-66-2017); by European Investment Funds by FEDER/COMPETE/POCI—Operational Competitiveness and Internationalization Program, and national funds by FCT—Portuguese Foundation for Science and Technology under projects UID/AGR/04033/2020, UIDB/CVT/00772/2020 and by Base Funding-UIDB/00511/2020 of the Laboratory for Process Engineering, Environment, Biotechnology, and Energy—LEPABE—funded by national funds through the FCT/MCTES (PID-DAC); Project 2SMART-engineered Smart materials for Smart citizens, with reference NORTE-01-0145-FEDER-000054, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.A growing proportion of oropharyngeal squamous cell carcinomas (OPSCC) are associated with infection by high-risk human papillomavirus (HPV). For reasons that remain largely unknown, HPV+OPSCC is significantly more common in men than in women. This study aims to determine the incidence of OPSCC in male and female HPV16-transgenic mice and to explore the role of female sex hormone receptors in the sexual predisposition for HPV+ OPSCC. The tongues of 30-weeks-old HPV16-transgenic male (n = 80) and female (n = 90) and matched wild-type male (n = 10) and female (n = 10) FVB/n mice were screened histologically for intraepithelial and invasive lesions in 2017 at the Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Por-tugal. Expression of estrogen receptors alpha (ERα) and beta (ERβ), progesterone receptors (PR) and matrix metalloproteinase 2 (MMP2) was studied immunohistochemically. Collagen remodeling was studied using picrosirius red. Female mice showed robust ERα and ERβ expression in intraepithelial and invasive lesions, which was accompanied by strong MMP2 expression and marked collagen remodeling. Male mice showed minimal ERα, ERβ and MMP2 expression and unaltered collagen patterns. These results confirm the association of HPV16 with tongue base cancer in both sexes. The higher cancer incidence in female versus male mice contrasts with data from OPSCC patients and is associated with enhanced ER expression via MMP2 upregulation.publishersversionpublishe

The protective effect of inflammatory monocytes during systemic C. albicans infection is dependent on collaboration between C-type lectin-like receptors

Acknowledgments The authors wish to acknowledge the NIH-sponsored Mutant Mouse Regional Resource Center (MMRRC) National System as the source of genetically-altered mice (C57BL/6-Clec4etm1.1Cfg/Mmucd 031936-UCD) for use in this study. The mice were produced and deposited to the MMRRC by the Consortium for Functional Glycomics supported by the National Institute of General Medical Sciences (GM62116). We would like to thank Catherine Neiseryan and Ann Kift-Morgan for cell sorting. We would like to thank Wales Gene Park for providing computer resources that assisted this research. Funding: SJO was funded by a Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society (Grant Number 099953/Z/12/Z) and by a Wellcome Trust ISSF Cross-Disciplinary Award. LCD is supported by a Henry Wellcome Trust Postdoctoral Fellowship (103973/Z/14/Z). CL is supported by a Kidney Research UK/MedImmune Joint Fellowship Award (PDF_006_20151127). GDB is funded by a Wellcome Trust Investigator Award (102705) and the MRC Centre for Medical Mycology and the University of Aberdeen (MR/N006364/1). IRH is supported by a Wellcome Trust Senior Research Fellowship (207503/Z/17/Z). PRT is supported by a Wellcome Trust Investigator Award (107964/Z/15/Z) and the UK Dementia Research Institute. Funding URLs: https://wellcome.ac.uk/ https://royalsociety.org/ https://www.kidneyresearchuk.org/ https://mrc.ukri.org/ https://ukdri.ac.uk/ The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Data Availability: All relevant data apart from RNAseq files are within the manuscript and its Supporting Information files. RNAseq data files are available from ArrayExpress (https://www.ebi.ac.uk/arrayexpress/), (accession number E-MTAB-8030).Peer reviewedPublisher PD

Corn hybrids height in different eucalyptus trees distances in systems agroforestry

Todos os textos, informa??es e resultados apresentados s?o de inteira responsabilidade dos autores.Objetivou-se avaliar as alturas de plantas de milho a diferentes dist?ncias das ?rvores de eucalipto em cons?rcio com capim-marandu, cultivado em diferentes arranjos de eucalipto em sistemas agrossilvipastoris, estabelecido h? cinco anos. Utilizou-se o delineamento experimental em blocos completos casualizados, com tr?s repeti??es e os tratamentos consistiram do arranjo fatorial (2x2)+2, sendo dois h?bridos de milho (duplo ? BM207, com folhas planiformes e triplo ? BM502, com folhas eretas) em cons?rcio com a Brachiaria brizantha cv. Marandu (capim-marandu), dois espa?amentos entre ?rvores de eucalipto (12x2 e 12x4 m), e os dois h?bridos de milho em monocultivo. Aos 110 dias ap?s a semeadura, ?poca de gr?os pastoso farin?ceo, foram determinadas as alturas de plantas de milho em tr?s diferentes distancias das ?rvores (2, 4 e 6 m) e determinada a m?dia entre as tr?s diferentes distancias, em cada unidade experimental. A altura dos h?bridos de milho foi influenciada pelas dist?ncias das ?rvores, pelos diferentes espa?amentos das ?rvores e entre os h?bridos utilizados nos sistemas em agrossilvipastoris j? estabelecidos h? cinco anos.ABSTRACT It was aimed to evaluate the height of corn plants at different distances from eucalyptus trees in consortium with marandugrass, grown in different eucalyptus arrangements in agroforestry systems, from five years ago. We used the experimental randomized complete block design, with three replications and the treatments consisted of a factorial arrangement (2x2) +2, two corn hybrids (double - BM207 with planiformes leaves and triple - BM502, with erect leaves) in consortium with Brachiariabrizantha cv. Marandu (marandugrass), two spacing between eucalyptus trees (12x2 and 12x4 meters), and the two corn hybrids in monoculture. At 110 days after sowing, period of pasty chalky grains, it was determined the height of corn plants in three different distances from the trees (2, 4 and 6 meters) and it was determined the average of the three different distances in each experimental unit. The height of the corn hybrids was influenced by the distances of the trees, by different spacing from the trees and among the hybrids used in the systems agroforestry from five years ago

Two-dimensional and three-dimensional techniques for determining the kinematic patterns for hindlimb obstacle avoidance during sheep locomotion

ABSTRACT: Analysis of locomotion is often used as a measure for impairment and recovery following experimental peripheral nerve injury. Compared to rodents, sheep offer several advantages for studying peripheral nerve regeneration. In the present study, we compared for the first time, two-dimensional (2D) and three-dimensional (3D) hindlimb kinematics during obstacle avoidance in the ovine model. This study obtained kinematic data to serve as a template for an objective assessment of the ankle joint motion in future studies of common peroneal nerve (CP) injury and repair in the ovine model. The strategy used by the sheep to bring the hindlimb over a moderately high obstacle, set to 10% of its hindlimb length, was pronounced knee, ankle and metatarsophalangeal flexion when approaching and clearing the obstacle. Despite the overall time course kinematic patterns about the hip, knee, ankle, and metatarsophalangeal were identical, we found significant differences between values of the 2D and 3D joint angular motion. Our results showed that the most apparent changes that occurred during the gait cycle were for the ankle (2D-measured STANCEmax: 157±2.4 degrees vs. 3D-measured STANCEmax: 151±1.2 degrees; P<.05) and metatarsophalangeal joints (2D-measured STANCEmin: 151±2.2 degrees vs. 3D-measured STANCEmin: 162 ± 2.2 degrees; P<.01 and 2D-measured TO: 163±4.9 degrees vs. 3D-measured TO: 177±1.4 degrees; P<.05), whereas the hip and knee joints were much less affected. Data and techniques described here are useful for an objective assessment of altered gait after CP injury and repairin an ovine model

A human Dectin-2 deficiency associated with invasive aspergillosis

Immunocompromised patients are highly susceptible to invasive aspergillosis. Herein, we identified a homozygous deletion mutation (507 del C) resulting in a frameshift (N170I) and early stop codon in the fungal binding Dectin-2 receptor, in an immunocompromised patient. The mutated form of Dectin-2 was weakly expressed, did not form clusters at/near the cell surface and was functionally defective. PBMCs from this patient were unable to mount a cytokine (TNF, IL-6) response to A. fumigatus and this first identified Dectin-2-deficient patient succumbed to invasive aspergillosis