2,285 research outputs found
Exploration of the Eucalyptus globulus gene pool
The first Europeans to discover Eucalyptus
globulus were French explorers in 1792. Its seed
was rapidly spread throughout the world in the
19th century and this was the species by which
much of the world first knew the genus.
However, it was in the industrial forests of the
20th century that this species, once considered
the ‘Prince of Eucalypts’, achieved greatest
prominence due to its fast growth and superior
pulp qualities. Formal breeding first commenced
in 1966 in Portugal and in the late 1980’s large
base population trials from open-pollinated seed
collections from native stands were established
in many countries. These trials have provided
unprecedented insights into the quantitative
genetic control of numerous traits of economic
and ecological importance and how this variation
is spatially distributed in the native range of the
species. However with large, fully pedigreed
breeding populations becoming available for
quantitative analysis and the rapidly expanding
knowledge of DNA sequence variation, we are
now at the threshold of a new understanding of
this important eucalypt gene pool. Indications of
the significance of non-additive genetic effects
are becoming available. The E. globulus
chloroplast genome has now been sequenced
and several genome maps have been published.
Studies of the variation in nuclear microsatellites
and the lignin biosynthesis gene CCR confirm
the complex, spatially structured nature of the
native gene pool. Strong spatial structuring of
the chloroplast genome has provided a tool for
tracking seed migration and the geographic
origin of exotic landraces. Highly divergent
lineages of chloroplast DNA have been
discovered and studies of the hypervariable JLA+
region argue that some components of the E.
globulus gene pool have been assimilated from
other species following hybridisation
Stellar Population Diagnostics of Elliptical Galaxy Formation
Major progress has been achieved in recent years in mapping the properties of
passively-evolving, early-type galaxies (ETG) from the local universe all the
way to redshift ~2. Here, age and metallicity estimates for local cluster and
field ETGs are reviewed as based on color-magnitude, color-sigma, and
fundamental plane relations, as well as on spectral-line indices diagnostics.
The results of applying the same tools at high redshifts are then discussed,
and their consistency with the low-redshift results is assessed. Most low- as
well as high-redshift (z~1) observations consistently indicate 1) a formation
redshift z>~3 for the bulk of stars in cluster ETGs, with their counterparts in
low-density environments being on average ~1-2 Gyr younger, i.e., formed at
z>~1.5-2, 2) the duration of the major star formation phase anticorrelates with
galaxy mass, and the oldest stellar populations are found in the most massive
galaxies. With increasing redshift there is evidence for a decrease in the
number density of ETGs, especially of the less massive ones, whereas existing
data appear to suggest that most of the most-massive ETGs were already fully
assembled at z~1. Beyond this redshift, the space density of ETGs starts
dropping significantly, and as ETGs disappear, a population of massive,
strongly clustered, starburst galaxies progressively becomes more and more
prominent, which makes them the likely progenitors to ETGs.Comment: To appear on Annual Review of Astronomy & Astrophysics, Vol. 44
(2006). 46 pages with 16 figures. Replaced version includes updated
references, few typos less, and replaces Fig. 11 and Fig. 16 which had been
skrewed u
The Precursors and Products of Justice Climates: Group Leader Antecedents and Employee Attitudinal Consequences
Drawing on the organizational justice, organizational climate, leadership and personality, and social comparison theory literatures, we develop hypotheses about the effects of leader personality on the development of three types of justice climates (e.g., procedural, interpersonal, and informational), and the moderating effects of these climates on individual level justice- attitude relationships. Largely consistent with the theoretically-derived hypotheses, the results showed that leader (a) agreeableness was positively related to procedural, interpersonal and informational justice climates, (b) conscientiousness was positively related to a procedural justice climate, and (c) neuroticism was negatively related to all three types of justice climates. Further, consistent with social comparison theory, multilevel data analyses revealed that the relationship between individual justice perceptions and job attitudes (e.g., job satisfaction, commitment) was moderated by justice climate such that the relationships were stronger when justice climate was high
Reference serving sizes for the Brazilian population: An analysis of processed food labels
Indisulam targets RNA splicing and metabolism to serve as a therapeutic strategy for high-risk neuroblastoma
Neuroblastoma is the most common paediatric solid tumour and prognosis remains poor for high-risk cases despite the use of multimodal treatment. Analysis of public drug sensitivity data showed neuroblastoma lines to be sensitive to indisulam, a molecular glue that selectively targets RNA splicing factor RBM39 for proteosomal degradation via DCAF15-E3-ubiquitin ligase. In neuroblastoma models, indisulam induces rapid loss of RBM39, accumulation of splicing errors and growth inhibition in a DCAF15-dependent manner. Integrative analysis of RNAseq and proteomics data highlight a distinct disruption to cell cycle and metabolism. Metabolic profiling demonstrates metabolome perturbations and mitochondrial dysfunction resulting from indisulam. Complete tumour regression without relapse was observed in both xenograft and the Th-MYCN transgenic model of neuroblastoma after indisulam treatment, with RBM39 loss, RNA splicing and metabolic changes confirmed in vivo. Our data show that dual-targeting of metabolism and RNA splicing with anticancer indisulam is a promising therapeutic approach for high-risk neuroblastoma
Analysis of tectonic-controlled fluvial morphology and sedimentary processes of the western Amazon Basin: an approach using satellite images and digital elevation model
Higher heritabilities for gait components than for overall gait scores may improve mobility in ducks
International audienceAbstractBackgroundGenetic progress in selection for greater body mass and meat yield in poultry has been associated with an increase in gait problems which are detrimental to productivity and welfare. The incidence of suboptimal gait in breeding flocks is controlled through the use of a visual gait score, which is a subjective assessment of walking ability of each bird. The subjective nature of the visual gait score has led to concerns over its effectiveness in reducing the incidence of suboptimal gait in poultry through breeding. The aims of this study were to assess the reliability of the current visual gait scoring system in ducks and to develop a more objective method to select for better gait.ResultsExperienced gait scorers assessed short video clips of walking ducks to estimate the reliability of the current visual gait scoring system. Kendall’s coefficients of concordance between and within observers were estimated at 0.49 and 0.75, respectively. In order to develop a more objective scoring system, gait components were visually scored on more than 4000 pedigreed Pekin ducks and genetic parameters were estimated for these components. Gait components, which are a more objective measure, had heritabilities that were as good as, or better than, those of the overall visual gait score.ConclusionsMeasurement of gait components is simpler and therefore more objective than the standard visual gait score. The recording of gait components can potentially be automated, which may increase accuracy further and may improve heritability estimates. Genetic correlations were generally low, which suggests that it is possible to use gait components to select for an overall improvement in both economic traits and gait as part of a balanced breeding programme
Cold gas accretion in galaxies
Evidence for the accretion of cold gas in galaxies has been rapidly
accumulating in the past years. HI observations of galaxies and their
environment have brought to light new facts and phenomena which are evidence of
ongoing or recent accretion:
1) A large number of galaxies are accompanied by gas-rich dwarfs or are
surrounded by HI cloud complexes, tails and filaments. It may be regarded as
direct evidence of cold gas accretion in the local universe. It is probably the
same kind of phenomenon of material infall as the stellar streams observed in
the halos of our galaxy and M31. 2) Considerable amounts of extra-planar HI
have been found in nearby spiral galaxies. While a large fraction of this gas
is produced by galactic fountains, it is likely that a part of it is of
extragalactic origin. 3) Spirals are known to have extended and warped outer
layers of HI. It is not clear how these have formed, and how and for how long
the warps can be sustained. Gas infall has been proposed as the origin. 4) The
majority of galactic disks are lopsided in their morphology as well as in their
kinematics. Also here recent accretion has been advocated as a possible cause.
In our view, accretion takes place both through the arrival and merging of
gas-rich satellites and through gas infall from the intergalactic medium (IGM).
The infall may have observable effects on the disk such as bursts of star
formation and lopsidedness. We infer a mean ``visible'' accretion rate of cold
gas in galaxies of at least 0.2 Msol/yr. In order to reach the accretion rates
needed to sustain the observed star formation (~1 Msol/yr), additional infall
of large amounts of gas from the IGM seems to be required.Comment: To appear in Astronomy & Astrophysics Reviews. 34 pages.
Full-resolution version available at
http://www.astron.nl/~oosterlo/accretionRevie
The Second Transmembrane Domain of P2X7 Contributes to Dilated Pore Formation
Activation of the purinergic receptor P2X7 leads to the cellular permeability of low molecular weight cations. To determine which domains of P2X7 are necessary for this permeability, we exchanged either the C-terminus or portions of the second transmembrane domain (TM2) with those in P2X1 or P2X4. Replacement of the C-terminus of P2X7 with either P2X1 or P2X4 prevented surface expression of the chimeric receptor. Similarly, chimeric P2X7 containing TM2 from P2X1 or P2X4 had reduced surface expression and no permeability to cationic dyes. Exchanging the N-terminal 10 residues or C-terminal 14 residues of the P2X7 TM2 with the corresponding region of P2X1 TM2 partially restored surface expression and limited pore permeability. To further probe TM2 structure, we replaced single residues in P2X7 TM2 with those in P2X1 or P2X4. We identified multiple substitutions that drastically changed pore permeability without altering surface expression. Three substitutions (Q332P, Y336T, and Y343L) individually reduced pore formation as indicated by decreased dye uptake and also reduced membrane blebbing in response to ATP exposure. Three others substitutions, V335T, S342G, and S342A each enhanced dye uptake, membrane blebbing and cell death. Our results demonstrate a critical role for the TM2 domain of P2X7 in receptor function, and provide a structural basis for differences between purinergic receptors. © 2013 Sun et al
The pharmacological regulation of cellular mitophagy
Small molecules are pharmacological tools of considerable value for dissecting complex biological processes and identifying potential therapeutic interventions. Recently, the cellular quality-control process of mitophagy has attracted considerable research interest; however, the limited availability of suitable chemical probes has restricted our understanding of the molecular mechanisms involved. Current approaches to initiate mitophagy include acute dissipation of the mitochondrial membrane potential (ΔΨm) by mitochondrial uncouplers (for example, FCCP/CCCP) and the use of antimycin A and oligomycin to impair respiration. Both approaches impair mitochondrial homeostasis and therefore limit the scope for dissection of subtle, bioenergy-related regulatory phenomena. Recently, novel mitophagy activators acting independently of the respiration collapse have been reported, offering new opportunities to understand the process and potential for therapeutic exploitation. We have summarized the current status of mitophagy modulators and analyzed the available chemical tools, commenting on their advantages, limitations and current applications
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