The Latin American society for developmental biology: A successful history

The Latin American Society for Developmental Biology (LASDB) is one of the newest societies in this field. However, despite being new, this society already had a highly important impact on the advancement of Developmental Biology across Latin America and globally. From its conception, the society began with the establishment of courses and congresses at the frontiers of knowledge and with the participation of researchers from Latin American countries and other regions, creating an academic and fraternal environment.The first LASDB congress was held in 2003, and recently, in 2019, the LASDB celebrated its tenth meeting, besides the Pan-American congress organized in 2007. Since the creation of this society and throughout its consolidation, the LASDB has been fortunate in receiving the support of highly prominent Developmental Biology societies, with which it has established links and collaboration that have clearly promoted Development Biology not only in Latin America but also in other parts of the world. At this moment, the LASDB looks to the future to continue supporting science in Latin America as it has done up to the present.Fil: Wappner, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Zurita, Mario Daniel. Universidad Nacional Autónoma de México. Instituto de Biotecnología; Méxic

Implementación de un módulo térmico para mejorar las condiciones de confort del laboratorio de diagnóstico técnico y eficiencia energética de la Facultad de Mecánica

El presente trabajo de titulación tuvo como objetivo implementar un módulo térmico para mejorar las condiciones de confort en el Laboratorio de Diagnóstico Técnico y Eficiencia Energética de la Facultad de Mecánica, planteado para fortalecer conocimientos acerca del funcionamiento de un sistema de refrigeración. El desarrollo del trabajo comprendió el cálculo de la carga térmica mediante el uso de un software, la selección de elementos y equipos en base a la carga de refrigeración y calefacción que permitieron el funcionamiento del equipo como un climatizador y la construcción del módulo térmico bajo el principio de funcionamiento de un ciclo ideal de refrigeración por compresión de vapor, se elaboró las guías de laboratorio para el módulo térmico, el manual de operación y el plan de mantenimiento. Por medio de elementos de control se realizó las pruebas de funcionamiento en donde el módulo térmico trabaja como un sistema de refrigeración o calefacción, permitiendo así una variación de temperatura en modo refrigeración de -3,4ºC en un lapso de tiempo de 24 minutos, obteniendo así una temperatura ambiente en el laboratorio de 20ºC, de la misma manera se realizó las pruebas de funcionamiento en modo calefacción, obteniendo una variación de temperatura de 4,3ºC en un tiempo de 20 minutos, logrando una temperatura ambiente en el laboratorio de 28,3ºC. Se concluye que el módulo térmico construido mantiene una temperatura ambiente en el laboratorio de 20ºC en modo refrigeración y una temperatura de 28,3ºC en modo calefacción y por medio de las guías de laboratorio se ayudó a la comprensión de un sistema básico de refrigeración, conociendo sus componentes principales, su principio de funcionamiento y cuáles son sus posibles fallas y averías. Para mayor eficiencia del módulo es recomendable un mayor aislamiento térmico para evitar el ingreso de temperaturas externas dentro del laboratorio.The objective of this research work was to implement a thermal module to improve comfort conditions in the Technical Diagnosis and Energy Efficiency Laboratory at Mechanics Faculty. It was proposed to strengthen knowledge about the operation of a refrigeration system. The development of the work included the calculation of the thermal load through the use of software, the selection of elements and equipment based on the cooling and heating load. They allowed the operation of the equipment as an air conditioner and the construction of the thermal module under the principle of operation of an ideal vapor compression refrigeration cycle. The laboratory guides for the thermal module, the operation manual and the maintenance plan were elaborated. By means of control elements, the performance tests were carried out where the thermal module works as a cooling or heating system, thus allowing a temperature variation in cooling mode of -3.4ºC in a time period of 24 minutes, obtaining thus, an ambient temperature in the laboratory of 20ºC. In the same way the operating tests were carried out in heating mode, obtaining a temperature variation of 4.3ºC in 20 minutes, achieving an ambient temperature in the laboratory of 28, 3ºC. It is concluded that the built thermal module maintains an ambient temperature in the laboratory of 20ºC in cooling mode and a temperature of 28.3ºC in heating mode. Using the laboratory guides the understanding of a basic refrigeration system was helped, knowing its main components, its principle of operation and what are its possible failures and breakdowns. For greater module efficiency, greater thermal insulation is recommended to prevent the entry of external temperatures of laboratory

Rediseño del Proceso de Producción del Yogurt Natural y Helado en la Unidad Empresarial de Base Combinado Lácteo Pinar del Río.

The following thesis work was carried out in the Enterprise of Dairy Products Pinar del Río. In the development of this investigation it was executed a redraw of the current situation of the process of production of the natural yogurt and ice cream, by means of which the lacks of each activity were analyzed. The general objective of the investigation is the redraw of the process of production of the Natural Yogurt and Ice cream, in which they were traced to carry out execution to this objective the following specific objectives: to define the theoretical and methodological aspects of the process administration, to establish the conceptual base for the redraw of process, to analyze and describe the productive process of the chosen products, as well as redrawing the production process. In the realization of the investigation they were used different methods and tools which facilitated the study and analysis of the production process, as well as the identification of the deficiencies found in the same ones. For they were made periodic visits to the company, for the summary of data by means of the interview and direct observation. It can also stand out the use of the diagrams of process flow (otida, operin), and journey diagram. Also the bibliographical revision by means of the consultation of documents and the search in internet.El siguiente trabajo de tesis se realizó en Unidad Empresarial de Base Combinado Lácteo Pinar del Río. En el desarrollo de esta investigación se realizó un rediseño de la situación actual del proceso de producción del Yogurt Natural y del Helado, mediante la cual se analizaron las carencias de cada actividad. El objetivo general de la investigación es: rediseñar el proceso de producción del Yogurt Natural y del Helado, y para dar cumplimiento a este objetivo se trazaron los siguientes objetivos específicos: definir los aspectos teóricos y metodológicos de la gestión por procesos; establecer la base conceptual para el rediseño de procesos, analizar y describir el proceso productivo de los productos lácteos Yogurt Natural y Helado, así como también rediseñar el proceso de producción de los mismos. En la realización de la investigación se utilizaron diferentes técnicas y herramientas las cuales facilitaron el estudio y análisis del proceso de producción, así como la identificación de las deficiencias encontradas en las mismas. Para ello se efectuaron visitas periódicas a la empresa, para la recopilación de datos mediante la entrevista y la observación directa. Además se puede destacar la utilización de los diagramas de flujo de proceso (otida, operin), y diagrama de recorrido

Impact of intrathecal cell therapy with autologous stromal cells on short-term memory binding in early Alzheimer's disease : one-year follow-up assessment of two cases

Background: We had previously reported that the administration of autologous stromal cells (ASCs) therapy to two patients with mild AD dementia led to a global increase in cerebral glucose metabolism which was accompanied by significant improvement of visual short-term memory binding (VSTMB), a function known to be a marker of AD. We suggested that intrathecal administration of autologous ASCs could be considered a new therapeutic strategy for AD dementia (Vaquero et al., 2019). We were interested in investigating the post-intervention durability of such cognitive improvements. Methods: We studied two AD patients with cerebral beta-amyloid neuritic plaques detected with 18FFDG-PET. The patients received every three months 100 million of ASCs by intrathecal route, until a total dose of 300 million. None received any other medication for its disease at the time of receiving cell therapy. Clinical and neuroimaging studies were performed previous and after the therapy, including brain glucose metabolism by 18F-FDG-PET and assessment with the visual short-term memory binding task (VSTMBT). This task has been proposed as a preclinical marker of AD. It requires subjects to detect whether or not two combinations of shape and colour change across two sequential arrays. Here we report on the assessment of these patients one year after the therapy. We compared them with 4 AD patients who did no undergo stem cell therapy. Results: Single case statistics revealed that benefits drawn by treated patients from the therapy remained a year after. Using a more taxing version of the VSTMB test (memory load of 3 items) we observed that, after the therapy, the chance that an untreated AD patient would show more impairment was 75.45% (p= 0.24) for Case 1 and 89.23% (p=0.11) for case 2. This chance remained after 1 year post-treatment for Case 1 (75.45%, p=0.24) and increased for Case 2 (96.89%, p=0.031). Conclusion: Improvements of memory functions known to be marker for AD in patients who underwent stem cell therapy remained stable after one year post-intervention. This offers a new therapeutic strategy for AD

Reflections on the possible applicability of the interventionist model of causation to psychoanalysis

El presente trabajo forma parte del proyecto de investigación “Debates epistemológicos y metodológicos en torno a la construcción de conocimiento en psicoanálisis” (Universidad Nacional de La Plata). Se desarrollarán los rasgos distintivos de la concepción intervencionista de la explicación y de la causalidad postulada por James Woodward que permiten pensar en su aplicabilidad al ámbito del psicoanálisis. Entre ellas se destaca la prescindibilidad de los enunciados legales en las explicaciones científicas, al postular la noción de invarianza bajo intervenciones y una concepción no reduccionista de la causalidad. Asimismo, postulamos que este modelo podría permitir trascender viejos, pero aun latentes, binomios -explicación/comprensión; causas/motivos- en la consideración de conocimiento psicoanalítico (Azcona y Lahitte, 2014).This paper is part of the research project “Epistemological and methodological debates about the construction of knowledge in psychoanalysis” (Universidad Nacional de La Plata). The distinctive features of the interventionist conception of the explanation and causality postulated by James Woodward will be developed, which allow us to think about its applicability to the field of psychoanalysis. Among them, it is worth mentioning the dispensability of legal statements in scientific explanations, by postulating the notion of invariance under interventions and a non-reductionist conception of causality. Likewise, we postulate that this model could allow to transcend old, but still latent, binomials -explanation/understanding; causes/motives- in the consideration of psychoanalytic knowledge (Azcona and Lahitte, 2014).Facultad de Psicologí

Generation of a human iPSC line from a patient with Leigh syndrome

Human iPSC line LND554SV.3 was generated from heteroplasmic fibroblasts of a patient with Leigh syndrome carrying a mutation in the MT-ND5 gene (m.13513G. >. A; p.D393N). Reprogramming factors Oct3/4, Sox2, Klf4, and cMyc were delivered using a non-integrative methodology that involves the use of Sendai virus.This work was supported by grants from the “Centro de Investigación Biomédica en Red en Enfermedades Raras” (CIBERER) (grant 13-717/132.05 to RG), the “Instituto de Salud Carlos III” [Fondo de Investigación Sanitaria and Regional Development Fund (ERDF/FEDER) funds PI10/0703 and PI13/00556 to RG and PI15/00484 to MEG], “Comunidad Autónoma de Madrid” (grant number S2010/BMD-2402 to RG); TG receives grant support from the Universidad Autónoma de Madrid (FPI-UAM) and FZD from the Ministerio de Educación, Cultura y Deporte (FPU13/00544). MEG is a staff scientist at the “Centro de Investigación Biomédica en Red en Enfermedades Raras” (CIBERER) at the “Centro de Investigación Biomédica en Red en Enfermedades Raras” (CIBERER)

Generation of a human control iPSC line with a European mitochondrial haplogroup U background

Human iPSC line N44SV.5 was generated from primary normal human dermal fibroblasts belonging to the European mitochondrial haplogroup U. For this purpose, reprogramming factors Oct3/4, Sox2, Klf4, and cMyc were delivered using a non-integrative methodology that involves the use of Sendai virus.This work was supported by grants from the “Centro de Investigación Biomédica en Red en enfermedades raras” (CIBERER) (grant 13-717/132.05 to RG), the “Instituto de Salud Carlos III” [Fondo de Investigación Sanitaria and Regional Development Fund (ERDF/FEDER) funds PI10/0703 and PI13/00556 to RG and PI15/00484 to MEG], “Comunidad Autónoma de Madrid” (grant number S2010/BMD-2402 to R.G); T.G. receives grant support from the Universidad Autónoma de Madrid, FPI-UAM and F.Z.D. from the Ministerio de Educación, Cultura y Deporte, grant number FPU13/00544. M.E.G. is staff scientist at the “Centro de Investigación Biomédica en Red en Enfermedades Raras” (CIBERER

Generation of a human iPSC line from a patient with a mitochondrial encephalopathy due to mutations in the GFM1 gene

Human iPSC line GFM1SV.25 was generated from fibroblasts of a child with a severe mitochondrial encephalopathy associated with mutations in the GFM1 gene, encoding the mitochondrial translation elongation factor G1. Reprogramming factors OCT3/4, SOX2, CMYC and KLF4 were delivered using a non integrative methodology that involves the use of Sendai virus.This work was supported by grants from the “Centro de Investigación Biomédica en Red en enfermedades raras” (CIBERER) (Grant 13-717/132.05 to RG), the “Instituto de Salud Carlos III” [Fondo de Investigación Sanitaria and Regional development fund (ERDF/FEDER) funds PI10/0703 and PI13/00556 to RG and PI15/00484 to MEG], “Comunidad Autónoma de Madrid” (Grant number S2010/BMD-2402 to RG); TG receives grant support from the Universidad Autónoma de Madrid (FPI-UAM) and FZD from the Ministerio de Educación, Cultura y Deporte (Grant FPU13/00544). MEG is staff scientist at the “Centro de Investigación Biomédica en Red en Enfermedades Raras” (CIBERER