Enhanced Capillary Rise of Wetting Liquids in Reduced Gravitational Shielding Under Microgravity Conditions

We study the capillary rise of wetting in liquids by slightly modifying Ponomarenko’s result, a recently derived and observed t1/3 law, without omitting the corresponding gravity term and therefore we find hnew (t)≅0.9085hPon (t) instead, which corresponds to a 9% difference. Furthermore, in order to examine the effect of corrected gravity, we extend the result on the surface of a planetary body by correcting the gravitational acceleration for its oblateness coefficient and rotation. We find that experiments that take place on the equator result in highest capillary heights, than those at mid latitudes and the poles. Similarly, analyzing the effect of reduced gravitational shielding on the capillary rise under conditions of microgravity in experiments aboard orbiting spacecraft, we find that equatorial circular orbits exhibit the highest capillary heights, where equatorial elliptical orbits of large eccentricities exhibit the smallest capillary heights. Finally, we calculate the rate of change of the meniscus height in the time domain, for the different laboratory conditions on the surface of the Earth and in space and as example. As an example we say that laboratory sites at the equator will exhibit larges time rates of change for the meniscus height. Similarly, for experiments above a spacecraft we find that circular equatorial orbits exhibit the highest time rates of change, where elliptical orbits exhibit smaller time rates that reduce as the eccentricity increases

Acceleration of weakly collisional solar-type winds

One of the basic properties of the solar wind, that is the high speed of the fast wind, is still not satisfactorily explained. This is mainly due to the theoretical difficulty of treating weakly collisional plasmas. The fluid approach implies that the medium is collision dominated and that the particle velocity distributions are close to Maxwellians. However the electron velocity distributions observed in the solar wind depart significantly from Maxwellians. Recent kinetic collisionless models (called exospheric) using velocity distributions with a suprathermal tail have been able to reproduce the high speeds of the fast solar wind. In this letter we present new developments of these models by generalizing them over a large range of corona conditions. We also present new results obtained by numerical simulations that include collisions. Both approaches calculate the heat flux self-consistently without any assumption on the energy transport. We show that both approaches - the exospheric and the collisional one - yield a similar variation of the wind speed with the basic parameters of the problem; both produce a fast wind speed if the coronal electron distribution has a suprathermal tail. This suggests that exospheric models contain the necessary ingredients for the powering of a transonic stellar wind, including the fast solar one.Comment: Accepted for publication in The Astrophysical Journal Letters (accepted: 13 May 2005

Application of a MHD hybrid solar wind model with latitudinal dependences to Ulysses data at minimum

In a previous work, Ulysses data was analyzed to build a complete axisymmetric MHD solution for the solar wind at minimum including rotation and the initial flaring of the solar wind in the low corona. This model has some problems in reproducing the values of magnetic field at 1 AU despite the correct values of the velocity. Here, we intend to extend the previous analysis to another type of solutions and to improve our modelling of the wind from the solar surface to 1 AU. We compare the previous results to those obtained with a fully helicoidal model and construct a hybrid model combining both previous solutions, keeping the flexibility of the parent models in the appropriate domain. From the solar surface to the Alfven, point, a three component solution for velocity and magnetic field is used, reproducing the complex wind geometry and the well-known flaring of the field lines observed in coronal holes. From the Alfven radius to 1 AU and further, the hybrid model keeps the latitudinal dependences as flexible as possible, in order to deal with the sharp variations near the equator and we use the helicoidal solution, turning the poloidal streamlines into radial ones. Despite the absence of the initial flaring, the helicoidal model and the first hybrid solution suffer from the same low values of the magnetic field at 1 AU. However, by adjusting the parameters with a second hybrid solution, we are able to reproduce both the velocity and magnetic profiles observed by Ulysses and a reasonable description of the low corona, provided that a certain amount of energy deposit exists along the flow. The present paper shows that analytical axisymmetric solutions can be constructed to reproduce the solar structure and dynamics from 1 solar radius up to 1 AU.Comment: 12 pages, 16 figure

DNA barcoding of nematodes using the MinION

Many nematode species are parasitic and threaten the health of plants and animals, including humans, on a global scale. Advances in DNA sequencing techniques have allowed for the rapid and accurate identification of many organisms including nematodes. However, the steps taken from sample collection in the field to molecular analysis and identification can take many days and depend on access to both immovable equipment and a specialized laboratory. Here, we present a protocol to genetically identify nematodes using 18S SSU rRNA sequencing using the MinION, a portable third generation sequencer, and proof that it is possible to perform all the molecular preparations on a fully portable molecular biology lab – the Bentolab. We show that both parasitic and free-living nematode species (Anisakis simplex, Panagrellus redivivus, Turbatrix aceti, and Caenorhabditis elegans) can be identified with a 96–100% accuracy compared to Sanger sequencing, requiring only 10–15 min of sequencing. This protocol is an essential first step toward genetically identifying nematodes in the field from complex natural environments (such as feces, soil, or marine sediments). This increased accessibility could in turn improve global information of nematode presence and distribution, aiding near-real-time global biomonitoring

Photoperiod-Dependent Expression of MicroRNA in Drosophila.

Like many other insects in temperate regions, Drosophila melanogaster exploits the photoperiod shortening that occurs during the autumn as an important cue to trigger a seasonal response. Flies survive the winter by entering a state of reproductive arrest (diapause), which drives the relocation of resources from reproduction to survival. Here, we profiled the expression of microRNA (miRNA) in long and short photoperiods and identified seven differentially expressed miRNAs (dme-mir-2b, dme-mir-11, dme-mir-34, dme-mir-274, dme-mir-184, dme-mir-184*, and dme-mir-285). Misexpression of dme-mir-2b, dme-mir-184, and dme-mir-274 in pigment-dispersing, factor-expressing neurons largely disrupted the normal photoperiodic response, suggesting that these miRNAs play functional roles in photoperiodic timing. We also analyzed the targets of photoperiodic miRNA by both computational predication and by Argonaute-1-mediated immunoprecipitation of long- and short-day RNA samples. Together with global transcriptome profiling, our results expand existing data on other Drosophila species, identifying genes and pathways that are differentially regulated in different photoperiods and reproductive status. Our data suggest that post-transcriptional regulation by miRNA is an important facet of photoperiodic timing

Local energy transfer rate and kinetic processes: the fate of turbulent energy in two-dimensional Hybrid Vlasov-Maxwell numerical simulations

The nature of the cross-scale connections between the inertial range turbulent energy cascade and the small-scale kinetic processes in collisionless plasmas is explored through the analysis of two-dimensional Hybrid Vlasov-Maxwell numerical simulation (HVM), with α particles, and through a proxy of the turbulent energy transfer rate, namely the Local Energy Transfer rate (LET). Correlations between pairs of variables, including those related to kinetic processes and to deviation from Maxwellian distributions, are first evidenced. Then, the general properties and the statistical scaling laws of the LET are described, confirming its reliability for the description of the turbulent cascade and revealing its textured topology. Finally, the connection between such proxy and the diag- nostic variables is explored using conditional averaging, showing that several quantities are enhanced in the presence of large positive energy flux, and reduced near sites of neg- ative flux. These observations can help determining which processes are involved in the dissipation of energy at small scales, as for example ion-cyclotron or mirror instabilities typically associated with perpendicular anisotropy of temperature

Characterization and phylogeny of fungi isolated from industrial wastewater using multiple genes

The aim of this study was the isolation and molecular characterization of fungi from untreated refinery effluent by using multiple conserved genes. The Fungi isolated were characterized based on PCR amplification and genomic sequencing of the internal transcribed spacer region (ITS), partial β-tubulin (BenA), calmodulin (CaM), and RNA polymerase second large subunit (RPB2) genes, along with morphological characterization. The obtained sequences were subjected to BLAST analysis and the corresponding fungal isolates were assigned species names after comparison with representative sequences available in GenBank. Fifteen (15) Fungi species belonging to four genera of Aspergillus, Penicillium, Fusarium, and Trichoderma with Aspergillus as the predominant genus were identified. Therefore these genes should be used as molecular markers for species level identification of fungi (especially Aspergillus and Penicillium as proven in this study

Development and validation of anti-human Alpha synuclein DNA aptamer using computer modelling techniques—an in silico study

Biomarker detection strategies have, in recent years, been moving towards nucleic acid-based detection systems in the form of aptamers, short oligonucleotide sequences which have shown promise in pre-clinical and research settings. One such aptamer is M5-15, a DNA aptamer raised against human alpha synuclein (α-syn) the causative agent in Lewy body and Parkinson’s disease (PD) associated dementia. While this aptamer has shown promise, in silico methodologies have demonstrated a capacity to produce aptamers that have higher affinities for their targets than in vitro generated sequences. A Python script random generated library of DNA sequences were screened based on their thermodynamic stability with the use of DINAMelt server-QuickFold web server. The selected sequences were examined with MFold in order to generate secondary structure data that were used to produce 3D data with the use of RNA composer software. Further on, the structure was corrected and RNA was replaced with DNA and the virtual screening for α-syn aptamer took place with a series of molecular docking experiments with the use of CSD-Discovery-GOLD software. Herein we propose an alternative in silico generated aptamer we call TMG-79 which demonstrates greater affinity for the target compared to M5-15 (M5-15 = –15.9 kcal/mol, TMG-79 = –17.77 kcal/mol) as well as better ChemPLP fitness scoring between the top poses (M5-15 = 32.33, TMG-79 = 53.32). Structural analysis suggests that while there are similarities, the greater potential flexibility of TMG-79 could be promoting greater affinity for the α-syn compared to M5-15. In silico methods of aptamer generation has the potential to revolutionise the field of aptamer design. We feel that further development of TMG-79 and validation in vitro will make it a viable candidate for diagnostic and research use in the future

The Application of PCR and STR DNA Profiling for the Identification of Haematoxylin Eosin Histological Slides in a Case of Sample Mix-Up Involving Synonymous Patients

A good laboratory practice ensures that biopsy material is correctly identified and associated with a given patient. Nevertheless, there are cases where the proof of origin of a tissue sample may be questioned. In this case study we have identified the source of cervical cancer glass slide sections stained with H/E, (hematoxylin eosin), after the request of a patient of Northern Greek origin who suspected sample mix-up when she coincidentally found out that a synonymous patient was examined for cervical cancer at the same time period in the same hospital in Greece. The patient was prepared to legally challenge the administrators of the downstream chemotherapeutic regimen. A combination of organic gradient clean up and silica membrane method was used for DNA isolation. Powerplex-16® system (Promega U.S.A) was used to generate complete DNA profiles from histological slides and the reference blood sample collected from the patient. Histochemical slides often yield inadequate STR profiles for successful DNA typing. Complete profiling in this case could be attributed to the adequate removal of stain and fixatives inhibitors and the isolation of good quality DNA for PCR or STR, protocols. Matching of histochemical slide DNA with patient blood DNA prevented legal action

The Solar Orbiter Science Activity Plan: translating solar and heliospheric physics questions into action

Solar Orbiter is the first space mission observing the solar plasma both in situ and remotely, from a close distance, in and out of the ecliptic. The ultimate goal is to understand how the Sun produces and controls the heliosphere, filling the Solar System and driving the planetary environments. With six remote-sensing and four in-situ instrument suites, the coordination and planning of the operations are essential to address the following four top-level science questions: (1) What drives the solar wind and where does the coronal magnetic field originate?; (2) How do solar transients drive heliospheric variability?; (3) How do solar eruptions produce energetic particle radiation that fills the heliosphere?; (4) How does the solar dynamo work and drive connections between the Sun and the heliosphere? Maximising the mission"s science return requires considering the characteristics of each orbit, including the relative position of the spacecraft to Earth (a ecting downlink rates), trajectory events (such as gravitational assist manoeuvres), and the phase of the solar activity cycle. Furthermore, since each orbit"s science telemetry will be downloaded over the course of the following orbit, science operations must be planned at mission level, rather than at the level of individual orbits. It is important to explore the way in which those science questions are translated into an actual plan of observations that fits into the mission, thus ensuring that no opportunities are missed. First, the overarching goals are broken down into specific, answerable questions along with the required observations and the so-called Science Activity Plan (SAP) is developed to achieve this. The SAP groups objectives that require similar observations into Solar Orbiter Observing Plans (SOOPs), resulting in a strategic, top-level view of the optimal opportunities for science observations during the mission lifetime. This allows for all four mission goals to be addressed. In this paper, we introduce Solar Orbiter"s SAP through a series of examples and the strategy being followed.Agencia Estatal de Investigació