1,298 research outputs found
Laser-uv-microirradiation (1= 257 nm) of Chinese hamster cells: evidence of uv-induced chromosome aberrations which do not originate at the sites of photolesions in the chromatin
Testing the neutrality of matter by acoustic means in a spherical resonator
New measurements to test the neutrality of matter by acoustic means are
reported. The apparatus is based on a spherical capacitor filled with gaseous
SF excited by an oscillating electric field. The apparatus has been
calibrated measuring the electric polarizability. Assuming charge conservation
in the decay of the neutron, the experiment gives a limit of
for the electron-proton charge
difference, the same limit holding for the charge of the neutron. Previous
measurements are critically reviewed and found incorrect: the present result is
the best limit obtained with this technique
Cooperative motion and growing length scales in supercooled confined liquids
Using molecular dynamics simulations we investigate the relaxation dynamics
of a supercooled liquid close to a rough as well as close to a smooth wall. For
the former situation the relaxation times increase strongly with decreasing
distance from the wall whereas in the second case they strongly decrease. We
use this dependence to extract various dynamical length scales and show that
they grow with decreasing temperature. By calculating the frequency dependent
average susceptibility of such confined systems we show that the experimental
interpretation of such data is very difficult.Comment: 7 pages of Latex, 3 figure
Confinement effects on glass forming liquids probed by DMA
Many molecular glass forming liquids show a shift of the glass transition T-g
to lower temperatures when the liquid is confined into mesoporous host
matrices. Two contrary explanations for this effect are given in literature:
First, confinement induced acceleration of the dynamics of the molecules leads
to an effective downshift of T-g increasing with decreasing pore size. Second,
due to thermal mismatch between the liquid and the surrounding host matrix,
negative pressure develops inside the pores with decreasing temperature, which
also shifts T-g to lower temperatures. Here we present dynamic mechanical
analysis measurements of the glass forming liquid salol in Vycor and Gelsil
with pore sizes of d=2.6, 5.0 and 7.5 nm. The dynamic complex elastic
susceptibility data can be consistently described with the assumption of two
relaxation processes inside the pores: A surface induced slowed down relaxation
due to interaction with rough pore interfaces and a second relaxation within
the core of the pores. This core relaxation time is reduced with decreasing
pore size d, leading to a downshift of T-g proportional to 1/d in perfect
agreement with recent differential scanning calorimetry (DSC) measurements.
Thermal expansion measurements of empty and salol filled mesoporous samples
revealed that the contribution of negative pressure to the downshift of T-g is
small (<30%) and the main effect is due to the suppression of dynamically
correlated regions of size xi when the pore size xi approaches
Direct recording of action potentials of cardiomyocytes through solution processed planar electrolyte-gated field-effect transistors
To achieve intracellular recording of action potentials by using simple devices that can be easily fabricated and processed is crucial in cardiology and neuroscience. Present tools and technology include invasive patch clamp technique, 3D nanostructures often combined with electro/opto poration methods and nanodevices such as nanowire field-effect transistors. However, these approaches mostly require complex manufacturing processes or are invasive. In this work, we report the spontaneous intracellular-like recording of cardiac cells using a cost-effective, planar Electrolyte-Gated Field-Effect Transistor (EGFET) based on solution-processed polymer-wrapped monochiral semiconducting single-walled carbon nanotubes (SWCNTs). By simply turning on the transistor, spontaneous recordings of intracellular-like action potentials of human induced pluripotent stem cells derived cardiomyocytes are enabled. In addition, we demonstrate that the same planar EGFET can also be employed as a platform for electroporation with significant device performance and cell viability. The simplicity of the device combined with the high signal to noise ratio opens up new opportunities for low-cost, reliable, and flexible biosensors and arrays for high quality parallel recording of cellular action potentials
Variability of aerosol, gaseous pollutants and meteorological characteristics associated with changes in air mass origin at the SW Atlantic coast of Iberia
Measurements of the ambient aerosol were performed at the Southern coast of Spain, within the framework of the DOMINO (<b>D</b>iel <b>O</b>xidant <b>M</b>echanisms <b>I</b>n relation to <b>N</b>itrogen <b>O</b>xides) project. The field campaign took place from 20 November until 9 December 2008 at the atmospheric research station "El Arenosillo" (37°5'47.76" N, 6°44'6.94" W). As the monitoring station is located at the interface between a natural park, industrial cities (Huelva, Seville) and the Atlantic Ocean, a variety of physical and chemical parameters of aerosols and gas phase could be characterized in dependency on the origin of air masses. Backwards trajectories were examined and compared with local meteorology to classify characteristic air mass types for several source regions. Aerosol number and mass as well as polycyclic aromatic hydrocarbons and black carbon concentrations were measured in PM<sub>1</sub> and size distributions were registered covering a size range from 7 nm up to 32 μm. The chemical composition of the non-refractory submicron aerosol (NR-PM<sub>1</sub>) was measured by means of an Aerosol Mass Spectrometer (Aerodyne HR-ToF-AMS). Gas phase analyzers monitored various trace gases (O<sub>3</sub>, SO<sub>2</sub>, NO, NO<sub>2</sub>, CO<sub>2</sub>) and a weather station provided meteorological parameters. <br><br> Lowest average submicron particle mass and number concentrations were found in air masses arriving from the Atlantic Ocean with values around 2 μg m<sup>−3</sup> and 1000 cm<sup>−3</sup>. These mass concentrations were about two to four times lower than the values recorded in air masses of continental and urban origins. For some species PM<sub>1</sub>-fractions in marine air were significantly larger than in air masses originating from Huelva, a closely located city with extensive industrial activities. The largest fraction of sulfate (54%) was detected in marine air masses and was to a high degree not neutralized. In addition, small concentrations of methanesulfonic acid (MSA), a product of biogenic dimethyl sulfate (DMS) emissions, could be identified in the particle phase. <br><br> In all air masses passing the continent the organic aerosol fraction dominated the total NR-PM<sub>1</sub>. For this reason, using Positive Matrix Factorization (PMF) four organic aerosol (OA) classes that can be associated with various aerosol sources and components were identified: a highly-oxygenated OA is the major component (43% OA) while semi-volatile OA accounts for 23%. A hydrocarbon-like OA mainly resulting from industries, traffic and shipping emissions as well as particles from wood burning emissions also contribute to total OA and depend on the air mass origin. <br><br> A significant variability of ozone was observed that depends on the impact of different air mass types and solar radiation
Mass-spectrometric identification of primary biological particle markers and application to pristine submicron aerosol measurements in Amazonia
The detection of primary biological material in submicron aerosol by means of thermal desorption/electron impact ionization aerosol mass spectrometry was investigated. Mass spectra of amino acids, carbohydrates, small peptides, and proteins, all of which are key building blocks of biological particles, were recorded in laboratory experiments. Several characteristic marker fragments were identified. The intensity of the marker signals relative to the total organic mass spectrum allows for an estimation of the content of primary biological material in ambient organic aerosol. The developed method was applied to mass spectra recorded during AMAZE-08, a field campaign conducted in the pristine rainforest of the central Amazon Basin, Brazil, during the wet season of February and March 2008. The low abundance of identified marker fragments places upper limits of 7.5% for amino acids and 5.6% for carbohydrates on the contribution of primary biological aerosol particles (PBAP) to the submicron organic aerosol mass concentration during this time period. Upper limits for the absolute submicron concentrations for both compound classes range from 0.01 to 0.1 μg m−3. Carbohydrates and proteins (composed of amino acids) make up for about two thirds of the dry mass of a biological cell. Thus, our findings suggest an upper limit for the PBAP mass fraction of about 20% to the submicron organic aerosol measured in Amazonia during AMAZE-08
Absolute Quantification of sp Defects in Semiconducting Single-Wall Carbon Nanotubes by Raman Spectroscopy
The functionalization of semiconducting single-wall carbon nanotubes (SWCNTs) with luminescent sp defects creates red-shifted emission features in the near-infrared and boosts their photoluminescence quantum yields (PLQYs). While multiple synthetic routes for the selective introduction of sp defects have been developed, a convenient metric to precisely quantify the number of defects on a SWCNT lattice is not available. Here, we present a direct and simple quantification protocol based on a linear correlation of the integrated Raman D/G+ signal ratios and defect densities as extracted from PLQY measurements. Corroborated by a statistical analysis of single-nanotube emission spectra at cryogenic temperature, this method enables the quantitative evaluation of sp defect densities in (6,5) SWCNTs with an error of ±3 defects per micrometer and the determination of oscillator strengths for different defect types. The developed protocol requires only standard Raman spectroscopy and is independent of the defect configuration, dispersion solvent, and nanotube length
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