5 research outputs found
Vertical Segregation and Phylogenetic Characterization of Ammonia-Oxidizing Bacteria and Archaea in the Sediment of a Freshwater Aquaculture Pond
Highly Efficient Electrochemiluminescence Resonance Energy Transfer System in One Nanostructure: Its Application for Ultrasensitive Detection of MicroRNA in Cancer Cells
The
electrochemiluminesce (ECL) efficiency of luminous emitter
can be enhanced by the means of electrochemiluminesce resonance energy
transfer (ECL-RET) with a matched donor. However, generally, the donor
and acceptor pairs were separated in different independent nanostructures,
experiencing the challenging issues of limited energy transfer efficiency
and luminous stability. Herein, we designed novel ECL-RET model within
one nanostructure containing the donor of trisÂ(4,4′-dicarboxylicacid-2,2′-bipyridyl)
rutheniumÂ(II) dichloride (RuÂ(dcbpy)<sub>3</sub><sup>2+</sup>) and
the acceptor of CdSe@ZnS quantum dots (QDs) for acting as the ECL
emitter (QDs-RuÂ(dcbpy)<sub>3</sub><sup>2+</sup>), which significantly
reduced the energy loss and improved the ECL efficiency of QDs because
of the short path of energy transmission. To demonstrate the proof-of-concept,
the proposed QDs-RuÂ(dcbpy)<sub>3</sub><sup>2+</sup> was employed to
construct a new kind of ECL biosensor that could achieve the ultrasensitive
detection of microRNA-141 (miRNA-141) combining target recycling amplification
and the double-output conversion strategies. Notably, the proposed
double-output conversion strategy enabled a small number of miRNA
to be successfully transferred into a large number of reporter DNA
which could capture numerous QDs-RuÂ(dcbpy)<sub>3</sub><sup>2+</sup>-labeled signal probes on the sensing surface to realize the ECL
response to the logarithm of the concentration of miRNA-141. With
the ultrahigh-efficient ECL-RET in one nanostructure and the dual
amplification including target recycling as well as double-output
conversion strategies, the proposed biosensor realized ultrasensitive
detection of miRNA-141 and performed the concentration range from
100 aM to 10 pM and the estimated detection limit was 33 aM (<i>S</i>/<i>N</i> = 3). Impressively, this method can
sensitively detect the miRNA-141 of human prostate cancer cells and
provide a significant boost for the detection of other biomarkers
in early cancer diagnosis and therapeutic monitoring