Bioactive constituents and shelf-life of sweet potato (Ipomoea batatas L.) leaves

We aimed to evaluate the green biomass’ of sweet potato (Ipomoea batatas L.) quality, through quantitative analysis of microelements, colour characteristics, and UHPLC-MS screening of bioactive constituents. The shelf life examination included sealed raw sweet potato leaves in plastic packs were stored at 6°C and 12°C and the microbiological characteristics were monitored for 2 weeks, through enumeration of mesophilic total plate count, total fungi count, Enterobacteriaceae and mesophilic aerobic spores. We found, that the sweet potato leaves can be considered as the source of calcium, magnesium and phosphorus among the minerals, of which calcium is the most abundant. We identified 17 types of amino acids, 7 vitamins, mainly vitamins belonging to the Vitamin B family. Furthermore, it contained carboxylic acids, flavonoids, polyphenols and aromatic compounds. The sweet potato leaves stored at 6°C was of satisfactory microbiological quality on day 14. Our data suggest that the sweet potato leaves could be a valuable source for healthy nutrition

The medicinal halophyte frankenia laevis l. (Sea Heath) has in vitro antioxidant activity, α-glucosidase inhibition, and cytotoxicity towards hepatocarcinoma cells

This work explored the medicinal halophyte Frankenia laevis L. (sea heath) as a potential source of bioactive natural products. In this sense, methanol and dichloromethane extracts were prepared from aerial organs containing flowers, leaves and stems, and were profiled for their chemical composition using high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS/MS). The extracts were evaluated for their in vitro antioxidant capacity using five complementary methods: enzyme inhibitory effects on enzymes related with neurodegeneration (acetyl (AChE) and butyrylcholinesterase (BuChE)), Type 2 diabetes (α-glucosidase and α-amylase), hyperpigmentation/food oxidation (tyrosinase), and cytotoxicity towards human hepatocarcinoma (HepG2) cells. Fifty-one molecules were identified in the extracts, including several derivatives of phenolic acids, lignans and flavonoids, monoterpenes, and hydroxylated derivatives of linoleic acid. The methanol extract was effective in DPPH and ABTS radical scavenging (EC50 = 0.25 and 0.65 mg/mL, respectively), copper chelation (EC50 = 0.78 mg/mL), and iron reduction (EC50 = 0.51 mg/mL) activities, whereas the dichloromethane extract had high iron chelating ability (EC50 = 0.76 mg/mL). Both extracts showed the capacity to inhibit α-glucosidase, especially the dichloromethane (EC50 = 0.52 mg/mL). This extract also exerted a significant selective cytotoxicity towards HepG2 cells (EC50 = 52.1 µg/mL, SI > 1.9). In conclusion, extracts from the aerial parts of sea heath were shown to be a promising source of natural products for pharmaceutical and/or food additive applications due to their high antioxidant, anti-diabetic, and cytotoxic properties.info:eu-repo/semantics/publishedVersio

Antioxidant activity, total flavonoid, total phenolic and anthocyanin contents of Cynara scolymus L. leaves and Trigonella foenum-graecum L. seeds

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A Simple Method for On-Gel Detection of Myrosinase Activity

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Comparative chemomapping of phytoconstituents from different extracts of globe artichoke - Cynara scolymus L.

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Identification of Bioactive Phytochemicals in Leaf Protein Concentrate of Jerusalem Artichoke (Helianthus tuberosus L.)

Jerusalem artichoke (JA) is widely known to have inulin-rich tubers. However, its fresh aerial biomass produces significant levels of leaf protein and economic bioactive phytochemicals. We have characterized leaf protein concentrate (JAPC) isolated from green biomass of three Jerusalem artichoke clones, Alba, Fuseau, and Kalevala, and its nutritional value for the human diet or animal feeding. The JAPC yield varied from 28.6 to 31.2 g DM k

HPLC-MS analysis of the active ingredients of chamomile (Matricaria chamomilla) bee feeding syrup

Apiarists let prepare by bee honey products containing medicinal drugs. Our aim was to prove that the active ingredients originated from the herbs are also present in the bee products. This publication focuses only to the chamomile (Matricaria chamomilla) containing feeding syrup and the difference between the various syrup phases. To fulfil this task, we developed a method analysing the non-volatile components of the syrup using the flowers of chamomile. The method involves a filtration followed by HPLC-MS analysis. The analyses and the data evaluations proved that the non-volatile components of the herb were transferred to syrup. As the characteristic components of certain herbs could be identified also in the syrup, it is obvious that the components responsible for the medicinal effects are also transferred

Nutritional and phyto-therapeutic value of the Halophyte Cladium mariscus L. (Pohl.): a special focus on seeds

This work searched for the phyto-therapeutic potential and nutritional value of seeds from the halophyte Cladium mariscus L. (Pohl.), aiming at its use as a source of bioactive ingredients for the food industry. Hence, the nutritional profile, including minerals, of seeds biomass was determined; food-grade samples were prepared, and their phytochemical fingerprinting assessed. Extracts were evaluated for in vitro antioxidant potential, inhibitory capacity towards enzymes related to neuroprotection, diabetes, and hyperpigmentation, and anti-inflammatory properties, along with a toxicological assessment. Sawgrass seeds can be considered a proper nutritional source with a good supply of minerals. All extracts had a high level of total phenolics (65.3–394.4 mg GAE/g DW) and showed a chemically rich and diverse profile of metabolites that have several biological properties described (e.g., antioxidant, anti-inflammatory). Extracts had no significant toxicity (cell viabilities > 80%) and were overall strong antioxidants (particularly at radical scavenging and reducing iron), effective tyrosinase inhibitors (55–71 mg KAE/g DW), showed anti-inflammatory properties (30–60% NO decrease), and had moderate capacity to inhibit enzymes related to neuroprotection (AChE 3.7–4.2, BChE 4.3–6.0 mg GALE/g DW) and diabetes (α-glucosidase 1.0–1.1, α-amylase 0.8–1.1 mmol ACAE/g). Altogether, results suggest that sawgrass seeds have the potential to be exploited as a new food product and are a reservoir of bioactive molecules with prospective applications as ingredients for value-added, functional, and/or preservative food productsinfo:eu-repo/semantics/publishedVersio