Hypothalamic gene expression during voluntary hypophagia in the Sprague-Dawley rat on withdrawal of the palatable liquid diet, Ensure

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A Guide for Children: Let\u27s Prepare for the Asylum Screening

Senior Project submitted to The Division of Social Studies of Bard College

Complexity, symmetry and variability of forward and backward walking at different speeds and transfer effects on forward walking: Implications for neural control

This study aimed to investigate effects of walking direction and speed on gait complexity, symmetry and variability as indicators of neural control mechanisms, and if a period of backward walking has acute effects on forward walking. Twenty-two young adults attended 2 visits. In each visit participants walked forwards at preferred walking speed (PWS) for 3-minutes (pre) followed by 5-minutes walking each at 80%, 100% and 120% of PWS of either forward or backward walking then a further 3-minutes walking forward at PWS (post). The order of walking speed in each visit was randomised and walking direction of each visit was randomised. An inertial measurement unit was placed over L5 vertebra to record tri-axial accelerations. From the trunk accelerations multiscale entropy, harmonic ratio and stride time variability were calculated to measure complexity, symmetry and variability for each walk. Complexity increased with increasing walking speed for all axes in forward and backward walking, and backward walking was less complex than forward walking. Stride time variability was also greater in backward than forward walking. Anterio-posterior and medio-lateral complexity increased following forward and backward walking but there was no difference between forward and backward walking post effects. No effects were found for harmonic ratio. These results suggest during backward walking trunk motion is rigidly controlled but central pattern generators responsible for temporal gait patterns are less refined for backward walking. However, in both directions complexity increased as speed increased suggesting additional constraint of trunk motion, normally characterised by reduced complexity, is not applied as speed increases

Microtopographic refuges enhance recruitment and survival, but inhibit growth of propagules of the tropical macroalga Sargassum swartzii

For organisms with discrete life-histories, any mechanism that enhances growth and/or survival at critical life-history transitions may significantly influence adult population size. On coral reefs, structurally complex microhabitats enhance the early post-settlement growth and/or survival of fishes and corals; however, the importance of such microhabitats to the early life stages of tropical macroalgae is largely unknown. Here, we investigate the effect of crevices on the recruitment, growth and survival of propagules of a common coral reef macroalga, Sargassum swartzii. We settled S. swartzii propagules onto terracotta settlement tiles that had a series of 3 mm deep crevices evenly spaced on their top (exposed) surface. Recruitment of S. swartzii was 21% greater, but propagules were 18% shorter, 18-days post-settlement within crevices than on adjacent exposed surfaces. Exposing tiles to local fish assemblages for five days showed that survival of propagules was 90% greater in crevices than on exposed areas of the tiles on the reef crest, but not on the reef flat. Underwater video footage revealed that few fishes fed from within the crevices (18% of all bites) with the majority of feeding being concentrated on the exposed surface of tiles. Interestingly, small-bodied fishes from the family Blenniidae (predominantly Ecsenius spp.) accounted for the majority of the feeding activity on the tiles, and likely contributed to the mortality of propagules. Structurally complex microhabitats, such as crevices, that shelter vulnerable early post-settlement propagules from herbivory may therefore be important for the persistence of macroalgae on coral reefs

A novel computerized test for detecting and monitoring visual attentional deficits and delirium in the ICU

Objectives: Delirium in the ICU is associated with poor outcomes but is under-detected. Here we evaluated performance of a novel, graded test for objectively detecting inattention in delirium, implemented on a custom-built computerized device (Edinburgh Delirium Test Box–ICU). Design: A pilot study was conducted, followed by a prospective case-control study. Setting: Royal Infirmary of Edinburgh General ICU. Patients: A pilot study was conducted in an opportunistic sample of 20 patients. This was followed by a validation study in 30 selected patients with and without delirium (median age, 63 yr; range, 23–84) who were assessed with the Edinburgh Delirium Test Box–ICU on up to 5 separate days. Presence of delirium was assessed using the Confusion Assessment Method for the ICU. Measurements and Main Results: The Edinburgh Delirium Test Box–ICU involves a behavioral assessment and a computerized test of attention, requiring patients to count slowly presented lights. Thirty patients were assessed a total of 79 times (n = 31, 23, 15, 8, and 2 for subsequent assessments; 38% delirious). Edinburgh Delirium Test Box–ICU scores (range, 0–11) were lower for patients with delirium than those without at the first (median, 0 vs 9.5), second (median, 3.5 vs 9), and third (median, 0 vs 10.5) assessments (all p < 0.001). An Edinburgh Delirium Test Box–ICU score less than or equal to 5 was 100% sensitive and 92% specific to delirium across assessments. Longitudinally, participants’ Edinburgh Delirium Test Box–ICU performance was associated with delirium status. Conclusions: These findings suggest that the Edinburgh Delirium Test Box–ICU has diagnostic utility in detecting ICU delirium in patients with Richmond Agitation and Sedation Scale Score greater than –3. The Edinburgh Delirium Test Box–ICU has potential additional value in longitudinally tracking attentional deficits because it provides a range of scores and is sensitive to change

Why do little kids ask to hear the same story over and over?

One way people learn new words is through reading books and stories. Little kids love hearing their favorite stories over and over and are also very good at learning new words. We wondered if reading the same stories could be helping preschool kids learn new words. Our research tested if it was better to read the same stories over and over or to read a few different stories. Here we tell you about three studies that show preschool kids learn more words from the same stories over and over. Our research suggests that it’s easier to learn new words from stories when you have heard the story before and know what is going to happen

The size, concentration, and growth of biodiversity-conservation nonprofits

Nonprofit organizations play a critical role in efforts to conserve biodiversity. Their success in this regard will be determined in part by how effectively individual nonprofits and the sector as a whole are structured. One of the most fundamental questions about an organization’s structure is how large it should be, with the logical counterpart being how concentrated the whole sector should be. We review empirical patterns in the size, concentration, and growth of over 1700 biodiversity-conservation nonprofits registered for tax purposes in the United States within the context of relevant economic theory. Conservation-nonprofit sizes vary by six to seven orders of magnitude and are positively skewed. Larger nonprofits access more revenue streams and hold more of their assets in land and buildings than smaller or midsized nonprofits do. The size of conservation nonprofits varies with the ecological focus of the organization, but the growth rates of nonprofits do not

Analysis of Photoreceptor Development in Pekin Duck

The Pekin duck is a valuable agricultural commodity in the U.S. Pekin ducks are seasonal breeders; they are sensitive to light and thus, research on the neuroendocrine and behavioral responses are needed to overcome technological limitations. There is compelling evidence that specific wavelengths of light are required to improve the growth and welfare of meat (grow out) ducks. For example, blue light may not be ideal for grow out ducks due to considerably increased motor activity, significantly decreased body weight and increased serum corticosterone (cort) levels. Therefore, our objective is to determine the role of both deep brain photoreceptors (DBPs) and retina photoreceptors (RPs) during duck development. Two groups of ducks were raised with and without light over 21 days from egg laying, embryonic day zero. We then collected brain and retinal tissues of ducks at embryonic days 3, 7, 11, 16, and 21. To examine DBPs, we designed and created primers for 4 genes: OPN4, VAL-opsin, OPN5, and rhodopsin. For RPs, we designed and created primers for genes responsible for both cones (RH2, SWS1, SWS2, LWS opsins) and rods (rhodopsin, MAFA, IRBP in duck eye development. qRT-PCR was performed utilizing listed primers for DBP, RP rods, RP cones and reference gene, using 10 samples each for ducks raised in both light and dark conditions. RNA was then extracted from the tissue collected and qRT-PCR was performed. Understanding when these specific genes are upregulated across development will help the husbandry of duck by providing an ideal time for light usage during duck development. Future directions will determine which wavelength of light, at a specific time of development, is most suitable for grow out ducks