Effect of interleukin-22 on immunogenicity of DNA vaccine encoding TSA gene of leishmania major in BALB/c mice

Background and purpose: Previous Research shows the use of plasmids containing genes TSA to be useful as vaccines for Leishmania major. Recently, the role of interleukin-22 (IL-22) in tissue repair has been demonstrated. In this research, the effect of IL-22 on encoding TSA gene of Leishmania major in BALB/c mice was assessed

Effects of dietary crude protein and calcium/ phosphorus content on growth, nitrogen and mineral retention in broiler chickens

This experiment was conducted to examine the effect of three levels of crude protein (CP) (NRC, 15% more than NRC and 15% less than NRC) and three levels of Ca and available P (Av. P) (NRC, 15% more than NRC and 15% less than NRC) on performance of broilers from hatching until 21 days of age. The experimental design was a completely randomized design (CRD), with a 3 × 3 factorial arrangement of nine dietary treatments. Each treatment combination had four replicate pens (10 birds per pen). Results of this experiment showed that CP content had no significant effect on feed and water intake. However, body weight gain (BWG) significantly reduced and FCR increased (P < 0.05) by 15% change in CP content of diet. Fifteen percent increase in mineral content of diets had no significant effect on feed intake. However, increase in Ca and Av. P significantly increased BWG and resulted in an improved feed conversion ratio (FCR) and increased water intake. There was an interactive effect of CP by Ca and Av. P levels on feed intake, BWG and FCR. A change in CP or mineral content of the diets had no significant effect on blood parameters except for potassium concentration (K+). Reducing CP, Ca and Av. P content of the diet significantly (P < 0.001) increased P, Ca and N retention. Reduction in CP content of the diet led to a decrease in length, P and tibia bone ash. There was an interactive effect of CP by Ca and Av. P on the retention of Ca, N and ash of tibia bone.Key words: Crude protein, Ca and Av. P levels, broilers, N retention

Sensitivity analysis on dynamic responses of geometrically imperfect base excited cantilevered beams

The non-linear non-planar dynamic responses of a near-square cantilevered geometrically imperfect (i.e., slightly curved) beam under harmonic primary resonant base excitation with a one-to-one internal resonance is investigated. By assuming two different geometric imperfection shapes, the sensitivity of the perfect beam model predicted limit-cycles to small geometric imperfections is analyzed by continuing them versus the imperfection parameter incorporating the imperfect beam model. This was carried out by assuming that the corresponding frequency detuning parameter associated with each limit-cycle is fixed. Also, other possible branches of dynamic solutions for the corresponding fixed detuning parameter within the interval of the imperfection amplitude are determined and the importance of accounting for the small geometric imperfections is discussed

Bearings coefficients effects on chaotic and bifurcation behavior of flexible rotor systems subjected to rub-impact

This study investigates the influence of end-support conditions on the chaotic and bifurcation behavior of a rotating flexible shaft-disk system. The system is modeled as a continuous shaft with a rigid disk in its mid span whilst supported by multi-coefficients bearings. Both Coriolis and centrifugal effects due to shaft flexibility are included. The partial differential equations of motion are extracted using the Rayleigh beam theory and the assumed mode method is used to discretize them in order to be solved numerically. The analytical tools used in this work include time series, phase plane portrait, power spectrum, Poincaré map, bifurcation diagrams, and Lyapunov exponents. The main objective of the present study is to investigate the effects of end-supports stiffness and damping coefficients on the chaotic vibration behavior of a rotating system. Periodic, sub-harmonic, quasi-periodic, and chaotic states have been observed for each case. As demonstrated, inclusion of the bearing effects can primarily change the speed ratios at which rub-impact occurs. The principal and cross-coupling stiffness and damping coefficients have quite different effects in the chaotic behavior of the system

MicroRNA-129-1 acts as tumour suppressor and induces cell cycle arrest of GBM cancer cells through targeting IGF2BP3 and MAPK1

Background MicroRNA-129-1 (miR-129-1) seems to behave as a tumour suppressor since its decreased expression is associated with different tumours such as glioblastoma multiforme (GBM). GBM is the most common form of brain tumours originating from glial cells. The impact of miR-129-1 downregulation on GBM pathogenesis has yet to be elucidated. Methods MiR-129-1 was overexpressed in GBM cells, and its effect on proliferation was investigated by cell cycle assay. MiR-129-1 predicted targets (CDK6, IGF1, HDAC2, IGF2BP3 and MAPK1) were also evaluated by western blot and luciferase assay. Results Restoration of miR-129-1 reduced cell proliferation and induced G1 accumulation, significantly. Several functional assays confirmed IGF2BP3, MAPK1 and CDK6 as targets of miR-129-1. Despite the fact that IGF1 expression can be suppressed by miR-129-1, through 30-untranslated region complementary sequence, we could not find any association between IGF1 expression and GBM. MiR-129-1 expression inversely correlates with CDK6, IGF2BP3 and MAPK1 in primary clinical samples. Conclusion This is the first study to propose miR129-1 as a negative regulator of IGF2BP3 and MAPK1 and also a cell cycle arrest inducer in GBM cells. Our data suggests miR-129-1 as a potential tumour suppressor and presents a rationale for the use of miR-129-1 as a novel strategy to improve treatment response in GBM

The effect of lavender essential oil and nanoemulsion on Trichomonas vaginalis in vitro

Background: Trichomonas vaginalis is the cause of trichomoniasis. Due to increased resistance and side effects of the drugs, the aim of this study was to assess an anti-trichomonias effect of lavender (Lavandula officinalis) essential oil and nanoemulsion on T. vaginalis in vitro. Materials and Methods: Lavender essential oil components were characterized by gas chromatography. To determine the cytotoxicity effects, the macrophage cell line J774.A.1 was used. Trichomonas vaginalis was isolated from vaginal secretions of the infected women and then cultured in the TYM complete medium and passaged for 10 days. The effect of essential oil and 1 lavender nanoemulsion in concentrations 10, 25, 50 and 100 μg/mL in the 24-well plate were examined at 1, 2 and 3 hours as triplicate. Positive control was metronidazole (50 μg/mL). The number of live and dead parasites was counted by trypan blue stain with a Neubauer slide. Results: The viability of the macrophages for lavender essential oil was 93.70 and for nanoemulsion was 90.90. Essential oil and nanoemulsion of lavender in concentration of 100 μg/mL and during 3 hours showed 81.7 and 81.9 growth inhibitory, respectively. This difference was not statistically significant. Conclusion: Lavender essential oil and nanoemulsion has a desirable inhibitory effect on growth of T.vaginalis and can be a good choice for conducting therapeutic investigations regarding trichomonial infections

Experimental and Finite Element Simulation of Wear in Nanostructured NiAl Coating

In this paper, the wear of nanostructured NiAl coating was studied both experimentally and numerically. First, the nanocrystalline NiAl intermetallic powder was synthesized by mechanical alloying (MA) of aluminum and Ni powders. The coatings were deposited onto the low carbon steel substrate using high velocity oxy-fuel (HVOF) technique. Nanoindentation test was conducted to find out the mechanical properties of the coating. The dry wear tests were then performed using a pin-on-block test rig under different operating conditions. Finally, finite element (FE) method was employed to model the wear characteristics of the prepared nanostructured material. A three-dimensional (3D) FE model was created and used to simulate the pin-on-block experiments. The results show that the volume losses predicted by the numerical analysis are in good agreement with the experimental data

Evaluation of individual and environmental sound pressure level and drawing noise-isosonic maps using Surfer V.14 and Noise at Work V.5.0

Noise pollution is one of the common physical harmful factors in many work environments. The current study aimed to assess personal and environmental sound pressure level and project the sound-Isosonic map in one of the Razavi Khorasan Paste manufacture using Surfer V.14 and Noise at work V.5.0. This cross-sectional, descriptive study is analytical that was conducted in 2018 in the Paste factory that contains Canister, production and Brewing unit. Following ISO 9612:2009, Casella Cel-320 was used to measure personal sound pressure level, while CEL-450 sound level meter (manufactured by Casella-Cel, the UK) was employed to assess environmental sound pressure level. Statistical analyzes was done using SPSS V.18 and Linear Regression test. The sound-isosonic maps were projected using Surfer V. 14 and Noise at work V.5.0. The results of assessing personal sound pressure level indicated that the highest received dose (172.21%) and personal equivalent sound level (87.36 dBA) were recorded for workers in the Canister unit. According to results of measuring of the environmental sound pressure level, out of 16 measurement stations in this unit, overall 87.5% were regarded as danger and caution areas. The lowest and highest sound pressure levels in this units were 61 dBA and 92 dBA that belong to Brewing and Canister units respectively. Results indicate Over 75% of the Canister and production units had a sound pressure level greater than 85 dBA and these two units were regarded as the most dangerous area in terms of noise pollution. It is therefore necessary to implement noise control measures, apply hearing protection program and auditory tests among workers in these units

A Generalization of Otsu's Method and Minimum Error Thresholding

We present Generalized Histogram Thresholding (GHT), a simple, fast, and effective technique for histogram-based image thresholding. GHT works by performing approximate maximum a posteriori estimation of a mixture of Gaussians with appropriate priors. We demonstrate that GHT subsumes three classic thresholding techniques as special cases: Otsu's method, Minimum Error Thresholding (MET), and weighted percentile thresholding. GHT thereby enables the continuous interpolation between those three algorithms, which allows thresholding accuracy to be improved significantly. GHT also provides a clarifying interpretation of the common practice of coarsening a histogram's bin width during thresholding. We show that GHT outperforms or matches the performance of all algorithms on a recent challenge for handwritten document image binarization (including deep neural networks trained to produce per-pixel binarizations), and can be implemented in a dozen lines of code or as a trivial modification to Otsu's method or MET.Comment: ECCV 202