Expression profiles, characterization and function of HbTCTP in rubber tree (Hevea brasiliensis)

As a highly conserved protein, the translationally controlled tumor protein (TCTP) carries out vital roles in various life processes. In rubber tree, two TCTP genes, HbTCTP and HbTCTP1, were cloned, but only HbTCTP1 was studied in details. In this study, cis-acting regulatory elements, expression patterns, subcellular localization, interacting proteins, and antioxidant activity of HbTCTP were systematically analyzed. Besides the common cis-acting regulatory elements, HbTCTP promoter also harbored various known cis-elements that respond to hormone/stresses. Being consistent with the aforementioned results, HbTCTP was regulated by drought, low temperature, high salt, ethylene (ET), wounding, H2O2, and methyl jasmonate (MeJA) treatments. HbTCTP was expressed throughout different tissues and developmental stages of leaves. In addition, HbTCTP was associated with tapping panel dryness (TPD). HbTCTP was localized in the membrane, cytoplasm and the nucleus, and interacted with four proteins rubber elongation factor (REF), 17.5 kDa heat shock family protein, annexin, and REF-like stress related protein 1. Being similar to HbTCTP1, HbTCTP also indicated antioxidant activity in metal-catalyzed oxidation (MCO) system. Our results are useful for further understanding the molecular characterization and expression profiles of HbTCTP, but also lay a solid foundation for elucidating the function of HbTCTP in rubber tree. (Résumé d'auteur

Cortical Neural Stem Cell Lineage Progression Is Regulated by Extrinsic Signaling Molecule Sonic Hedgehog.

Neural stem cells (NSCs) in the prenatal neocortex progressively generate different subtypes of glutamatergic projection neurons. Following that, NSCs have a major switch in their progenitor properties and produce γ-aminobutyric acid (GABAergic) interneurons for the olfactory bulb (OB), cortical oligodendrocytes, and astrocytes. Herein, we provide evidence for the molecular mechanism that underlies this switch in the state of neocortical NSCs. We show that, at around E16.5, mouse neocortical NSCs start to generate GSX2-expressing (GSX2+) intermediate progenitor cells (IPCs). In vivo lineage-tracing study revealed that GSX2+ IPC population gives rise not only to OB interneurons but also to cortical oligodendrocytes and astrocytes, suggesting that they are a tri-potential population. We demonstrated that Sonic hedgehog signaling is both necessary and sufficient for the generation of GSX2+ IPCs by reducing GLI3R protein levels. Using single-cell RNA sequencing, we identify the transcriptional profile of GSX2+ IPCs and the process of the lineage switch of cortical NSCs

CR-SFP: Learning Consistent Representation for Soft Filter Pruning

Soft filter pruning~(SFP) has emerged as an effective pruning technique for allowing pruned filters to update and the opportunity for them to regrow to the network. However, this pruning strategy applies training and pruning in an alternative manner, which inevitably causes inconsistent representations between the reconstructed network~(R-NN) at the training and the pruned network~(P-NN) at the inference, resulting in performance degradation. In this paper, we propose to mitigate this gap by learning consistent representation for soft filter pruning, dubbed as CR-SFP. Specifically, for each training step, CR-SFP optimizes the R-NN and P-NN simultaneously with different distorted versions of the same training data, while forcing them to be consistent by minimizing their posterior distribution via the bidirectional KL-divergence loss. Meanwhile, the R-NN and P-NN share backbone parameters thus only additional classifier parameters are introduced. After training, we can export the P-NN for inference. CR-SFP is a simple yet effective training framework to improve the accuracy of P-NN without introducing any additional inference cost. It can also be combined with a variety of pruning criteria and loss functions. Extensive experiments demonstrate our CR-SFP achieves consistent improvements across various CNN architectures. Notably, on ImageNet, our CR-SFP reduces more than 41.8\% FLOPs on ResNet18 with 69.2\% top-1 accuracy, improving SFP by 2.1\% under the same training settings. The code will be publicly available on GitHub.Comment: 11 pages, 4 figure

Genome-wide sequencing of small RNAs reveals a tissue-specific loss of conserved microRNA families in Echinococcus granulosus

Background: MicroRNAs (miRNAs) are important post-transcriptional regulators which control growth and development in eukaryotes. The cestode Echinococcus granulosus has a complex life-cycle involving different development stages but the mechanisms underpinning this development, including the involvement of miRNAs, remain unknown. Results: Using Illumina next generation sequencing technology, we sequenced at the genome-wide level three small RNA populations from the adult, protoscolex and cyst membrane of E. granulosus. A total of 94 pre-miRNA candidates (coding 91 mature miRNAs and 39 miRNA stars) were in silico predicted. Through comparison of expression profiles, we found 42 mature miRNAs and 23 miRNA stars expressed with different patterns in the three life stages examined. Furthermore, considering both the previously reported and newly predicted miRNAs, 25 conserved miRNAs families were identified in the E. granulosus genome. Comparing the presence or absence of these miRNA families with the free-living Schmidtea mediterranea, we found 13 conserved miRNAs are lost in E. granulosus, most of which are tissue-specific and involved in the development of ciliated cells, the gut and sensory organs. Finally, GO enrichment analysis of the differentially expressed miRNAs and their potential targets indicated that they may be involved in bi-directional development, nutrient metabolism and nervous system development in E. granulosus. Conclusions: This study has, for the first time, provided a comprehensive description of the different expression patterns of miRNAs in three distinct life cycle stages of E. granulosus. The analysis supports earlier suggestions that the loss of miRNAs in the Platyhelminths might be related to morphological simplification. These results may help in the exploration of the mechanism of interaction between this parasitic worm and its definitive and intermediate hosts, providing information that can be used to develop new interventions and therapeutics for the control of cystic echinococcosis

Exposing the (002) active facet by reducing surface energy for a high-performance Na3_3V2_2(PO4_4)2_2F3_3 cathode

Increasing the exposure of active crystal facets is an effective strategy to promote the transmission of Na+ through the electrolyte/cathode interface and is key to developing high-performance sodium-ion battery (SIB) cathodes. Here, Na$_3$V$_2$(PO$_4$)$_2$F$_3$ (NVPFs) were synthesized with diverse (002) active facet exposure rates, in which the growth rate of the different crystal facets was regulated by adjusting the surface energy. The growth mechanism of the NVPF crystals was proposed using density functional theory calculations. The NVPFs with 82% (002) active facet exposure delivered a remarkable reversible capacity of 123 mA h g$^{-1}$ and energy density of 431 W h kg$^{-1}$ in a full cell. Theoretical investigations of the Na+ adsorption, diffusion, and storage clarified that the exposed (002) crystal facet had more stable Na$^+$-storage sites, which resulted in a lower energy barrier during the Na$^+$-diffusion process. The promoted Na$^+$-transportation kinetics and related enhanced stability were further elucidated based on the combined analyses of synchrotron X-ray absorption spectroscopy and electrochemical measurements. This research aimed to develop a way to promote the transmission process of Na$^+$ through the electrolyte/cathode interface from the perspective of crystal facet regulation, which is expected to promote the commercial application of SIB cathodes

A Pilot Study for Control of Hyperendemic Cystic Hydatid Disease in China

Cystic hydatid disease (CHD), caused by the dog tapeworm, Echinococcus granulosus, is hyperendemic in western China. However, until recently the disease had been grossly neglected there due primarily to a weak economy and the primitive control measures used. The situation is now changing because of China's growing economy and the availability of the cheap and effective drug praziquantel (PZQ), which makes control programs practicable through mass preventive treatment of dogs in these endemic areas. In this study, we used a strategy to prevent parasite eggs from being released into the environment by dosing dogs monthly with baited PZQ and culling unwanted and stray dogs, as a result of which infection in humans and domestic animals can be reduced considerably. We undertook a pilot control study in two counties involving a population of 255,504 in 52,300 households and 30,380 dogs in Xinjiang, China, and showed that monthly PZQ treatment of dogs is an efficient, practicable, and affordable control method for communities to reduce considerably hyperendemic CHD. As a result, the Chinese Ministry of Health has extended the control measure to 117 counties in western China

The E3 ligase OsPUB15 interacts with the receptor-like kinase PID2 and regulates plant cell death and innate immunity

BACKGROUND: Rice blast disease is one of the most destructive diseases of rice worldwide. We previously cloned the rice blast resistance gene Pid2, which encodes a transmembrane receptor-like kinase containing an extracellular B-lectin domain and an intracellular serine/threonine kinase domain. However, little is known about Pid2-mediated signaling. RESULTS: Here we report the functional characterization of the U-box/ARM repeat protein OsPUB15 as one of the PID2-binding proteins. We found that OsPUB15 physically interacted with the kinase domain of PID2 (PID2K) in vitro and in vivo and the ARM repeat domain of OsPUB15 was essential for the interaction. In vitro biochemical assays indicated that PID2K possessed kinase activity and was able to phosphorylate OsPUB15. We also found that the phosphorylated form of OsPUB15 possessed E3 ligase activity. Expression pattern analyses revealed that OsPUB15 was constitutively expressed and its encoded protein OsPUB15 was localized in cytosol. Transgenic rice plants over-expressing OsPUB15 at early stage displayed cell death lesions spontaneously in association with a constitutive activation of plant basal defense responses, including excessive accumulation of hydrogen peroxide, up-regulated expression of pathogenesis-related genes and enhanced resistance to blast strains. We also observed that, along with plant growth, the cell death lesions kept spreading over the whole seedlings quickly resulting in a seedling lethal phenotype. CONCLUSIONS: These results reveal that the E3 ligase OsPUB15 interacts directly with the receptor-like kinase PID2 and regulates plant cell death and blast disease resistance. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-015-0442-4) contains supplementary material, which is available to authorized users

Investigation and genetic polymorphism analysis of rodents infected with Echinococcus in Ili Prefecture, Xinjiang Uygur Autonomous Region, China

IntroductionAlveolar echinococcosis (AE) is a life-threatening disease in humans caused by the larval stage of Echinococcus multilocularis. Domestic animals, dogs, foxes, and small mammals constitute the circular chain of AE. To evaluate the infection, distribution, and genetic polymorphism of AE in the Ili Prefecture (Nilka, Xinyuan and Zhaosu), we conducted this survey.MethodsIn June and July 2018, 267 small mammals were captured using water-infusion and mousetrap methods. Combined pathogenic and molecular biological methods were used to observe the histopathology of Echinococcus carried by rodents, amplify the mitochondrial nad1 gene of the pathogen, and investigate the genotype and haplotype diversity of Echinococcus in rodents in Ili Prefecture.ResultsMorphological identification revealed that these captured small mammals belonged to three species, with Microtus gregalis being the dominant species (183/267). Pathological and molecular biological results confirmed that E. multilocularis was the pathogen of echinococcosis in small mammals, with an infection rate of 15.73% (42/267). Among the three areas sampled, the highest infection rate of rodents was 25.45% (14/55) in Nilka County. However, there was no significant difference in the infection rates between regions (χ2 = 5.119, p > 0.05). Of the three captured rodent species, M. gregalis had the highest infection rate of 17.49% (32/183), but there was no significant difference in infection rates between the rodent species (χ2 = 1.364, p > 0.05). Phylogenetic analyses showed that the nad1 gene sequences obtained in this study clustered in the same clade as isolates from China. These isolates contained 21 haplotypes (Hap_1-21); Hap_2 was the most common haplotype (9/42). Furthermore, haplotype diversity (0.925 ± 0.027) and nucleotide diversity (0.01139 ± 0.00119) were higher in the Ili Prefecture than in other regions, indicating that population differentiation was high. Tajima’s D and Fu’s Fs tests were negative (p > 0.10), indicating that the population had expanded. The low fixation index (Fst) ranged from 0.00000 to 0.16945, indicating that the degree of genetic differentiation was different among different populations.DiscussionIn summary, Ili Prefecture is a high incidence area of AE, and Microtus spp. may play an important role in the transmission of AE in this area. The results of this study provide basic data for further study of the molecular epidemiology, genetic differences, and control of E. multilocularis in the Ili Prefecture, Xinjiang

Investigation into Photoconductivity in Single CNF/TiO2-Dye Core–Shell Nanowire Devices

A vertically aligned carbon nanofiber array coated with anatase TiO2 (CNF/TiO2) is an attractive possible replacement for the sintered TiO2 nanoparticle network in the original dye-sensitized solar cell (DSSC) design due to the potential for improved charge transport and reduced charge recombination. Although the reported efficiency of 1.1% in these modified DSSC’s is encouraging, the limiting factors must be identified before a higher efficiency can be obtained. This work employs a single nanowire approach to investigate the charge transport in individual CNF/TiO2 core–shell nanowires with adsorbed N719 dye molecules in dark and under illumination. The results shed light on the role of charge traps and dye adsorption on the (photo) conductivity of nanocrystalline TiO2 CNF’s as related to dye-sensitized solar cell performance

Probing Nucleation Mechanism of Self-Catalyzed InN Nanostructures

The nucleation and evolution of InN nanowires in a self-catalyzed growth process have been investigated to probe the microscopic growth mechanism of the self-catalysis and a model is proposed for high pressure growth window at ~760 Torr. In the initial stage of the growth, amorphous InNx microparticles of cone shape in liquid phase form with assistance of an InNx wetting layer on the substrate. InN crystallites form inside the cone and serve as the seeds for one-dimensional growth along the favorable [0001] orientation, resulting in single-crystalline InN nanowire bundles protruding out from the cones. An amorphous InNx sheath around the faucet tip serves as the interface between growing InN nanowires and the incoming vapors of indium and nitrogen and supports continuous growth of InN nanowires in a similar way to the oxide sheath in the oxide-assisted growth of other semiconductor nanowires. Other InN 1D nanostructures, such as belts and tubes, can be obtained by varying the InN crystallites nucleation and initiation process