S-adenosyl-L-methionine improves ventricular remodeling after myocardial infarction by regulating angiogenesis and fibrosis

Purpose: To investigate the effect of S-adenosyl-L-methionine (SAM) on angiogenesis and fibrosis in the heart of rats with myocardial infarction (MI), and to determine the mechanism of action.Methods: Sprague Dawley rats with MI received SAM treatment (15 mg/kg) intraperitoneally. The cumulative survival (%) of rats was recorded to determine their rate of survival. Hematoxylin-eosin staining, echocardiography, and hemodynamics were also performed. In addition, the effects of SAM vascular regeneration in the rats were analyzed by determining the expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and hypoxia-inducible factor 1-α (HIF1-α) in rats.Results: The 8-week survival rate of the MI group was significantly lower than that of the sham group, while SAM significantly improved the survival rate of the rats. In addition, SAM improved the contractile and diastolic heart function in the rats and also increased the ventricular pressure change. Furthermore, SAM elevated the expressions of VEGF, bFGF and HIF1-α in rat myocardium and serum. In myocardial tissues of SAM-treated rats, the expressions of collagen I, collagen III and α-sma were reduced, indicating that SAM inhibited myocardial fibrosis. In addition, SAM promoted cardiac angiogenesis by activating Jagged1/Notch1 signaling pathway.Conclusion: SAM promotes angiogenesis of the myocardium by activating Jagged1/Notch1 signaling pathway and inhibiting fibrosis in rat myocardium. Therefore, SAM effectively inhibits ventricular remodeling in rats after MI, thereby improving the rats’ heart structure and function. The results may provide new targets for the treatment of myocardial infarction

The complete mitochondrial genome of Scatoglyphus polytrematus (Acari: Acaridae)

We assembled and annotated the complete mitochondrial genome of Scatoglyphus polytrematus. It is the first complete mitochondrial genome sequence from the genus Scatoglyphus. The mitogenome was 13,966 bp in length and contains 37 genes (including 13 protein-coding genes, 22 transfer RNA (tRNA), and two ribosomal RNA (rRNA)), and one largest non-coding region. The gene arrangement of S. polytrematus is consistent with the pattern of possible common ancestor of astigmatid mites. In the present study, phylogenetic analysis shows that genus Scatoglyphus was clustered into one branch with other Acaridae species

Sedimentary characteristics and tectonic setting of sublacustrine fans in a half-graben rift depression, Beibuwan Basin, South China Sea

Drilling has shown that there is a large petroleum reserve in the Liushagang Formation of the Fushan Depression, South China Sea from reservoirs of the sublacustrine fans. To reduce the risk associated with exploration, the sedimentary characteristics of the sublacustrine fans should be identified and described. Analysis of the drilling cores, well data and 3D seismic data was conducted to identify and interpret the sublacustrine fans which formed in a lacustrine succession as the low-stand fan of the 2nd member of Liushagang Formation (SQEls2). The reservoirs were commonly associated with pebbly conglomerates, mudstone shivers, muddy debris, various deformation structures including micro-faults, liquified deformation structure, ball and pillow structure, slump and stress-release deformation. Further contrastive analysis of sedimentary characteristics and palaeogeomorphology between the western and eastern sublacustrine fans indicates that they belong to two different types of sublacustrine system in continental basins. More specifically, the western fan, controlled by the multi-level step-fault belt, is a typical gravel-rich sublacustrine system which is characterised by structurally controlled, high gradient (2.8-3.3°), admixtures of muddy debris and pebbly conglomerates and poor reservoir quality, while the eastern fan, controlled by the flexure slope-break belt, is a sand-rich sublacustrine system dominated by relatively low gradient (2.0-2.5°), general fining-upwards trend with good reservoir quality. Therefore, the development of future exploration should be conducted at different levels in the Fushan Depression

MHC class I links with severe pathogenicity in C57BL/6N mice infected with SARS-CoV-2/BMA8

Abstract Background The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes non-symptomatic infection, mild influenza-like symptoms to pneumonia, severe acute respiratory distress syndrome, and even death, reflecting different clinical symptoms of viral infection. However, the mechanism of its pathogenicity remains unclear. Host-specific traits have a breakthrough significance for studying the pathogenicity of SARS-CoV-2. We previously reported SARS-CoV-2/BMA8, a mouse-adapted strain, was lethal to aged BALB/c mice but not to aged C57BL/6N mice. Here, we further investigate the differences in pathogenicity of BMA8 strain against wild-type aged C57BL/6N and BALB/c mice. Methods Whole blood and tissues were collected from mice before and after BMA8 strain infection. Viral replication and infectivity were assessed by detection of viral RNA copies and viral titers; the degree of inflammation in mice was tested by whole blood cell count, ELISA and RT-qPCR assays; the pathogenicity of SARS-CoV-2/BMA8 in mice was measured by Histopathology and Immunohistochemistry; and the immune level of mice was evaluated by flow cytometry to detect the number of CD8+ T cells. Results Our results suggest that SARS-CoV-2/BMA8 strain caused lower pathogenicity and inflammation level in C57BL/6N mice than in BALB/c mice. Interestingly, BALB/c mice whose MHC class I haplotype is H-2Kd showed more severe pathogenicity after infection with BMA8 strain, while blockade of H-2Kb in C57BL/6N mice was also able to cause this phenomenon. Furthermore, H-2Kb inhibition increased the expression of cytokines/chemokines and accelerated the decrease of CD8+ T cells caused by SARS-CoV-2/BMA8 infection. Conclusions Taken together, our work shows that host MHC molecules play a crucial role in the pathogenicity differences of SARS-CoV-2/BMA8 infection. This provides a more profound insight into the pathogenesis of SARS-CoV-2, and contributes enlightenment and guidance for controlling the virus spread

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Spectral evolution of the coexistence patterns as a function of pump powe

D-LMBmap: a fully automated deep-learning pipeline for whole-brain profiling of neural circuitry.

Acknowledgements: This work was supported by the Medical Research Council, as part of United Kingdom Research and Innovation (UK Research and Innovation) (MC_UP_1201/22). For the purpose of open access, the Medical Research Council Laboratory of Molecular Biology has applied a CC BY public copyright license to any Author Accepted Manuscript version arising. This work was also partially funded by NARSAD Young Investigator Award (2020, BBRF) to J.R. and Ministry of Science and Technology (2022ZD0206700) and the Beijing Municipal Government of P.R.C. to R.L. We thank D. Friedmann for advice on Adipo-Clear, J. Kebschull and D. Friedmann for data sharing, and L. Luo, M. Hastings, A.M.J. Adams and J. Song for critique on the manuscript.Funder: Medical Research Council, as part of the United Kingdom Research and Innovation, MC_UP_1201/22Recent proliferation and integration of tissue-clearing methods and light-sheet fluorescence microscopy has created new opportunities to achieve mesoscale three-dimensional whole-brain connectivity mapping with exceptionally high throughput. With the rapid generation of large, high-quality imaging datasets, downstream analysis is becoming the major technical bottleneck for mesoscale connectomics. Current computational solutions are labor intensive with limited applications because of the exhaustive manual annotation and heavily customized training. Meanwhile, whole-brain data analysis always requires combining multiple packages and secondary development by users. To address these challenges, we developed D-LMBmap, an end-to-end package providing an integrated workflow containing three modules based on deep-learning algorithms for whole-brain connectivity mapping: axon segmentation, brain region segmentation and whole-brain registration. D-LMBmap does not require manual annotation for axon segmentation and achieves quantitative analysis of whole-brain projectome in a single workflow with superior accuracy for multiple cell types in all of the modalities tested

Additional file 3 of MHC class I links with severe pathogenicity in C57BL/6N mice infected with SARS-CoV-2/BMA8

Additional file 3 The sequence of Ace2 gene of BALB/c mice

Additional file 2 of MHC class I links with severe pathogenicity in C57BL/6N mice infected with SARS-CoV-2/BMA8

Additional file 2 The sequence of Ace2 gene of C57BL/6N mice