68 research outputs found
Expression profile of cuticular genes of silkworm, Bombyx mori
<p>Abstract</p> <p>Background</p> <p>Insect cuticle plays essential roles in many physiological functions. During molting and metamorphosis tremendous changes occur in silkworm cuticle where multiple proteins exist and genes encoding them constitute about 1.5% of all <it>Bombyx mori </it>genes.</p> <p>Results</p> <p>In an effort to determine their expression profiles, a microarray-based investigation was carried out using mRNA collected from larvae to pupae. The results showed that a total of 6676 genes involved in various functions and physiological pathways were activated. The vast majority (93%) of cuticular protein genes were expressed in selected stages with varying expression patterns. There was no correlation between expression patterns and the presence of conserved motifs. Twenty-six RR genes distributed in chromosome 22 were co-expressed at the larval and wandering stages. The 2 kb upstream regions of these genes were further analyzed and three putative elements were identified.</p> <p>Conclusions</p> <p>Data from the present study provide, for the first time, a comprehensive expression profile of genes in silkworm epidermal tissues and evidence that putative elements exist to allow massive production of mRNAs from specific cuticular protein genes.</p
Proteomic Analysis of Larval Midgut from the Silkworm (Bombyx mori)
The midgut is the major organ for food digestion, nutrient absorption and also a barrier for foreign substance. The 5th-instar larval stage of silkworm is very important for larval growth, development, and silk production. In the present study, we used 2-DE and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to analyze the midgut proteins from the 5th-instar larvae as well as the midgut proteins under starvation condition. A total of 96 proteins were identified in this study; and among them, 69 proteins were observed in midgut for the first time. We also found that the silkworm larval midgut responded to starvation by producing a 10 kDa heat shock protein and a diapause hormone precursor
MicroRNAs show diverse and dynamic expression patterns in multiple tissues of Bombyx mori
<p>Abstract</p> <p>Background</p> <p>MicroRNAs (miRNAs) repress target genes at the post-transcriptional level, and function in the development and cell-lineage pathways of host species. Tissue-specific expression of miRNAs is highly relevant to their physiological roles in the corresponding tissues. However, to date, few miRNAs have been spatially identified in the silkworm.</p> <p>Results</p> <p>We establish for the first time the spatial expression patterns of nearly 100 miRNAs in multiple normal tissues (organs) of <it>Bombyx mori </it>females and males using microarray and Northern-blotting analyses. In all, only 10 miRNAs were universally distributed (including bmo-let-7 and bmo-bantam), while the majority were expressed exclusively or preferentially in specific tissue types (e.g., bmo-miR-275 and bmo-miR-1). Additionally, we examined the developmental patterns of miRNA expression during metamorphosis of the body wall, silk glands, midgut and fat body. In total, 63 miRNAs displayed significant alterations in abundance in at least 1 tissue during the developmental transition from larvae to pupae (e.g., bmo-miR-263b and bmo-miR-124). Expression patterns of five miRNAs were significantly increased during metamorphosis in all four tissues (e.g., bmo-miR-275 and bmo-miR-305), and two miRNA pairs, bmo-miR-10b-3p/5p and bmo-miR-281-3p/5p, showed coordinate expression.</p> <p>Conclusions</p> <p>In this study, we conducted preliminary spatial measurements of several miRNAs in the silkworm. Periods of rapid morphological change were associated with alterations in miRNA expression patterns in the body wall, silk glands, midgut and fat body during metamorphosis. Accordingly, we propose that corresponding ubiquitous or tissue-specific expression of miRNAs supports their critical roles in tissue specification. These results should facilitate future functional analyses.</p
MicroRNA expression profiling during the life cycle of the silkworm (Bombyx mori)
<p>Abstract</p> <p>Background</p> <p>MicroRNAs (miRNAs) are expressed by a wide range of eukaryotic organisms, and function in diverse biological processes. Numerous miRNAs have been identified in <it>Bombyx mori</it>, but the temporal expression profiles of miRNAs corresponding to each stage transition over the entire life cycle of the silkworm remain to be established. To obtain a comprehensive overview of the correlation between miRNA expression and stage transitions, we performed a whole-life test and subsequent stage-by-stage examinations on nearly one hundred miRNAs in the silkworm.</p> <p>Results</p> <p>Our results show that miRNAs display a wide variety of expression profiles over the whole life of the silkworm, including continuous expression from embryo to adult (miR-184), up-regulation over the entire life cycle (let-7 and miR-100), down-regulation over the entire life cycle (miR-124), expression associated with embryogenesis (miR-29 and miR-92), up-regulation from early 3<sup>rd </sup>instar to pupa (miR-275), and complementary pulses in expression between miR-34b and miR-275. Stage-by-stage examinations revealed further expression patterns, such as emergence at specific time-points during embryogenesis and up-regulation of miRNA groups in late embryos (miR-1 and bantam), expression associated with stage transition between instar and molt larval stages (miR-34b), expression associated with silk gland growth and spinning activity (miR-274), continuous high expression from the spinning larval to pupal and adult stages (miR-252 and miR-31a), a coordinate expression trough in day 3 pupae of both sexes (miR-10b and miR-281), up-regulation in pupal metamorphosis of both sexes (miR-29b), and down-regulation in pupal metamorphosis of both sexes (miR-275).</p> <p>Conclusion</p> <p>We present the full-scale expression profiles of miRNAs throughout the life cycle of <it>Bombyx mori</it>. The whole-life expression profile was further investigated via stage-by-stage analysis. Our data provide an important resource for more detailed functional analysis of miRNAs in this animal.</p
Microarray-based gene expression profiles in multiple tissues of the domesticated silkworm, Bombyx mori
Using a genome-wide oligonucleotide microarray, gene expression was surveyed in multiple silkworm tissues on day 3 of the fifth instar, providing a new resource for annotating the silkworm genome
Propagation of travelling waves in sub-excitable systems driven by noise and periodic forcing
It has been reported that traveling waves propagate periodically and stably
in sub-excitable systems driven by noise [Phys. Rev. Lett. \textbf{88}, 138301
(2002)]. As a further investigation, here we observe different types of
traveling waves under different noises and periodic forces, using a simplified
Oregonator model. Depending on different noises and periodic forces, we have
observed different types of wave propagation (or their disappearance).
Moreover, the reversal phenomena are observed in this system based on the
numerical experiments in the one-dimensional space. As an explanation, we
regard it as the effect of periodic forces. Thus, we give qualitative
explanations to how reversal phenomena stably appear, which seem to arise from
the mixing function of the periodic force and the noise. And the output period
and three velocities (the normal, the positive and the negative) of the
travelling waves are defined and their relationship with the periodic forces,
along with the types of waves, are also studied in sub-excitable system under a
fixed noise intensity.Comment: Some references and information are added in the modified version.
Accepted, The European Physical Journal
Thermal Resonance in Signal Transmission
We use temperature tuning to control signal propagation in simple
one-dimensional arrays of masses connected by hard anharmonic springs and with
no local potentials. In our numerical model a sustained signal is applied at
one site of a chain immersed in a thermal environment and the signal-to-noise
ratio is measured at each oscillator. We show that raising the temperature can
lead to enhanced signal propagation along the chain, resulting in thermal
resonance effects akin to the resonance observed in arrays of bistable systems.Comment: To appear in Phys. Rev.
Environmental Metal Pollution Considered as Noise: Effects on the Spatial Distribution of Benthic Foraminifera in two Coastal Marine Areas of Sicily (Southern Italy)
We analyze the spatial distributions of two groups of benthic foraminifera
(Adelosina spp. + Quinqueloculina spp. and Elphidium spp.), along Sicilian
coast, and their correlation with six different heavy metals, responsible for
the pollution. Samples were collected inside the Gulf of Palermo, which has a
high level of pollution due to heavy metals, and along the coast of Lampedusa
island (Sicily Channel, Southern Mediterranean), which is characterized by
unpolluted sea waters. Because of the environmental pollution we find: (i) an
anticorrelated spatial behaviour between the two groups of benthic foraminifera
analyzed; (ii) an anticorrelated (correlated) spatial behaviour between the
first (second) group of benthic foraminifera with metal concentrations; (iii)
an almost uncorrelated spatial behaviour between low concentrations of metals
and the first group of foraminifera in clean sea water sites. We introduce a
two-species model based on the generalized Lotka-Volterra equations in the
presence of a multiplicative noise, which models the interaction between
species and environmental pollution due to the presence in top-soft sediments
of heavy metals. The interaction coefficients between the two species are kept
constant with values in the coexistence regime. Using proper values for the
initial conditions and the model parameters, we find for the two species a
theoretical spatial distribution behaviour in a good agreement with the data
obtained from the 63 sites analyzed in our study.Comment: 28 pages, 8 figures, 5 table
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