The practice exploration of case teaching mode in the environmental monitoring course teaching

In view of that short and strong practical nature of the core courses in the environmental monitor, and the shortcomings of the case teaching modes of the project completion environmental protection acceptance case in the present individual application project, the project completion acceptance actual case method is set up in the course of the course of the course of environmental monitoring, and the practical case of the project completion acceptance test for the project completion of the project completion of the project is set up in the practical case, which is proved by the practice of the project completion environmental protection acceptance monitoring report of the packaging material of jiangsu shentai packaging material co., LTD. The project has been done with the study of the environment and the teaching model of the project, and it has been applied to the students as the main subject, and it has been taught to do a better job in monitoring the environment after graduation. Keywords Environmental monitoring; The acceptance of the environmental protection project case; The teaching method DOI: 10.7176/JEP/10-6-1

The Effect of Calcium and Phosphorous on Growth, Feed Efficiency, Mineral Content and Body Composition of Brown Marbled Grouper (Epinephelus Fuscoguttatus) Juvenile

The objectives of this study were to know concentration of calcium (Ca) and posphorus (P) in feed for growth, feed efficiency, proximate composition of the body and mineral content of brown marbled grouper juvenile. The study was conducted in the Center for Brackiswater Aquaculture Development, Takalar with randomized completed design 6 x 3 with the treatment of Ca and P supplement in feed i.e., (A) the supplement of 0 g/kg Ca and 0 g/kg P, (B) the supplement of 6 g/kg Ca and 0 g/kg P, (C) the supplement of 0 g/kg Ca and 6 g/kg P, (D) the supplement of 6 g/kg Ca and 6 g/kg P, (E) the supplement of 12 g/kg Ca and 6 g/kg P, and (F) the supplement of 18 g/kg Ca and 6 g/kg P. The result showed that P supplement with doses of 6 g/kg and Ca of 0 g/kg in feed are significantly affects on relative growth, feed efficiency, proximate composition and mineral content of brown marbled grouper juvenile

Exploration of Training Mode of Applied Talents in Environmental Engineering Specialty

According to the characteristics of environmental engineering in universities, combined with the characteristics of social requirements, a new type of environmental engineering specialty curriculum system is constructed. The curriculum system agrees with the position of running a school and aims of personnel training in the application- oriented universities, providing practical and suitable way to cultivate high- level application- oriented talents for the market and production. Keywords:Application teaching, Environmental engineering specialty, curriculum syste

Synergy of arsenic with smoking in causing cardiovascular disease mortality: A cohort study with 27 follow-up years in China

BackgroundTo explore the patterns of the exposure-response relationship between arsenic exposure and cardiovascular disease (CVD) mortality and investigate the effect of cigarette smoking on the association.MethodsSeven thousand seven hundred thirty-five tin miners with at least 10 years of arsenic exposure were enrolled since 1992 and followed up for 27 years. Each individual's air arsenic exposure at workplace was calculated by time weighted average arsenic concentration × exposure months. Detailed information on smoking was collected at baseline, and information on smoking status was collected for five consecutive years from 1992 to 1996. Hazard ratio (HR) and 95% confidence interval (CI) for the risk of CVD were estimated using Cox proportional hazards models.ResultsA total of 1,046 CVD deaths occurred in this cohort over 142,287.7 person-years of follow up. We firstly reported that for equal cumulative exposure, participants exposed to higher concentrations over shorter duration had a higher risk of CVD mortality than those exposed to lower concentration over longer duration. The HR and 95% CI were 1.38 (95%CI: 1.03–1.85) in participants exposed to arsenic concentration (45.5–99.5 mg/m3), 1.29 (95%CI: 1.02–1.67) in 99.5–361.0 mg/m3. Further, participants with age at first exposure <18 years had a significantly higher risk of morality from CVD, cerebrovascular and heart diseases than those with ≥18 years. Finally, all synergy indices were greater than 1 (range, 1.11–2.39), indicating that the joint effect of arsenic exposure and cigarette smoking on CVD mortality was greater than the sum of their individual effect.ConclusionsExposure to air arsenic at workplace is adversely associated with mortality from CVD, especially among smokers younger than 18 years and smokers

Recombinant immunotoxin anti-c-Met/PE38KDEL inhibits proliferation and promotes apoptosis of gastric cancer cells

<p>Abstract</p> <p>Background</p> <p>Our study aims to evaluate the anti-growth effects of recombinant immunotoxin (IT) anti-c-Met/PE38KDEL on gastric cancer cells, and its mechnisms.</p> <p>Methods</p> <p>Gastric cancer cells were treated with increasing doses of IT and c-Met protein was quantified by Western blotting. Cell proliferation was determined by Cell Counting Kit-8 assay (CCK). [³H]-leucine incorporation assay was used to evaluate IT inhibition of protein synthesis. Cell apoptosis was quantified by flow cytometry. Caspase activities were measured using colorimetric protease assays.</p> <p>Results</p> <p>Cell growth and protein synthesis of the gastric cancer cell lines were suppressed by IT in a dose- and time-dependent manner. IT also induced apoptosis in a dose-dependent manner. The apoptosis rates of gastric cancer cell lines MKN-45 and SGC7901 were 19.19% and 27.37%, respectively when treated with 50 ng/ml of IT. There were significant increase ofcaspase-3 activity at 24 hr of IT treatment (100 ng/ml) (P < 0.01) in these gastric cancer cell lines.</p> <p>Conclusions</p> <p>IT anti-c-Met/PE38KDEL has anti-growth effects on the gastric cancer cell lines <it>in vitro</it>, and it provides an experimental basis for c-Met-targeted therapy towards <it>in vivo </it>testing.</p

Effects of triazolodiazepine on the production of interleukin-6 from murine spleen cells and rabbit synovial cells in vitro

Interleukin-6 (IL-6) is a multifunctional cytokine that regulates the immune response, acute phase anaphylactic reaction, and haematopoiesis. Lipopolysaccharide (6–24 μg/ml) significantly induced IL-6 release from murine spleen cells. In cultured rabbit synovial cells interleukin-1 (IL-1, 1–10 U/ml) induced IL-6 production in a concentration-dependent manner. Triazolodiazepine (Tri) is a hetrazepine platelet-activating factor antagonist. In this study we found that Tri (0.1–10 μmol/l) exerted strong inhibitory effects on LPS stimulated IL-6 production in murine spleen cells. Kinetic studies showed that the inhibition of IL-6 release was time-independent. In rabbit synovial cells Tri also reduced IL-6 release induced by IL-1 and tumour necrosis factor. Inhibition of cytokine production by Tri may partially explain its wide and strong anti-inflammatory effects

Buyang Huanwu Decoction Targets SIRT1/VEGF Pathway to Promote Angiogenesis After Cerebral Ischemia/Reperfusion Injury

Background: Ischemia stroke is known as the major cause of morbidity and mortality. Buyang Huanwu Decoction (BHD), a classical traditional Chinese medicine (TCM) formula, has been used to prevent and treat stoke for hundreds of years. The purpose of present study is to investigate the effects of BHD on angiogenesis in rats after cerebral ischemia/reperfusion (I/R) injury targeting Silent information regulator 1 (SIRT1) / Vascular endothelial growth factor (VEGF) pathway.Methods: The cerebral I/R injury model was induced by middle cerebral artery occlusion (MCAO). Adult Sprag-Dawley (SD) rats were randomly divided into five groups: sham group, normal saline (NS) group, BHD group, BHD+EX527 (SIRT1 specific inhibitor) group, and NS+EX527 group. Each group was divided into the subgroups according to 1, 3, 7, or 14 days time-point after cerebral ischemia/reperfusion, respectively. Neurological function score (NFS) was evaluated by the Rogers scale; microvascular density (MVD) in brain tissue around infarction area was observed by immunofluorescence; and the expression of SIRT1 and VEGF was assessed by Western Blot and Quantitative Real-time-PCR.Results: BHD can significantly improve NFS (P &lt; 0.05), increase the MVD in the boundary ischemic area (P &lt; 0.01) and elevate the expression of protein and mRNA of SIRT1 and VEGF following I/R injury (P &lt; 0.01). In contrast, treatment with EX527 reversed the BHD-induced improvements in NFS (P &lt; 0.01) and decreased the MVD (P &lt; 0.01) and the expression of SIRT1 and VEGF (P &lt; 0.05).Conclusion: BHD exerts neuroprotection targeting angiogenesis through the up-regulation of SIRT1/VEGF pathway against cerebral ischemic injury in rats