Bone Metastases in Patients with Prostate Cancer: A Literature Review

Prostate cancer is a major threat to men's health around the world. Bone is the most common metastasis site in patients with prostate cancer, which may lead to bone pain, pathological fracture and spinal cord compression, and it is related to various physiological or pathological factors such as age, physical condition, and previous treatment. The occurrence of bone-related events will seriously affect the quality of life of patients. In recent years, more and more people are concerned about bone metastasis of prostate cancer. Some important international cancer organizations have issued guidelines for the diagnosis and treatment of prostate cancer with bone metastasis. However, Clinicians still have some misunderstandings about bone metastasis of prostate cancer, especially selection of therapeutic strategies and the screening of appropriate drugs. Nowadays, the therapeutic strategies of prostate cancer with bone metastases mainly include primary lesions and bone metastases lesions. The former include surgery, radiotherapy, endocrine therapy, chemotherapy, immunotherapy, radiofrequency ablation and so on. And the later is made up of bone modification drug, radiopharmaceutical, lifestyle adjustment, and symptomatic analgesic treatment. The purpose of this review is to summarize the status and progression of bone metastasis in prostate cancer and to explore the best diagnosis and therapeutic strategies

Gravitation Field Algorithm with Optimal Detection for Unconstrained Optimization

This work is supported by the National Natural Science Foundation of China (Grant Nos. 61472159, 61572227), Development Project of Jilin Province of China (Nos. 20160204022GX, 20160414009GH, 2017C033).Postprin

Construction of Method for Rapid Detection of Vibrio Parahaemolyticus Using the Quantitative Real-Time PCR Based on the ToxR Gene

Abstract: In this research, a sensitive, rapid and highly reproducible SYBR green based real-time PCR assay was developed for detection of toxR positive pathogenic Vibrio parahaemolyticus. To establish a real-time PCR assay for accurate and rapid detection of Vibrio parahaemolyticus. The special target sequence of toxR gene of Vibrio parahaemolyticus was amplified and characterized with a pair of primes. Reaction system and determination approach of real-time PCR were established for detection of Vibrio parahaemolyticus. The foodborne pathogen of Staphylococcus aureus, Salmonella were used as specificity reference for the method, the amplification curves were observed as a typical "S" curve, other pathogens were also tested and no amplification was observed. In addition, the results of melting curve analysis showed only a specific peak with a melting temperature of 86.33°C and no primerdimers peak was observed. These findings indicated that the PCR primers had high specificity. Analysis of standard curves revealed excellent correlation between the number of copies (in the range of 4×10 9 to 4×10 1 and PCR threshold cycle (Ct) with a correlation coefficient of 0.992 (R 2 = 0.992). It was found that the limit of this assay was 15 CFU/mL for pure culture and 1.535pg for genomic DNA. The total detection assay could be completed in 2 h. Results indicated that real-time PCR detection methods established in this research was accurate, sensitive, rapid, reproducible for the quantitative detection of environmental Vibrio parahaemolyticus

Grade-control outdoor turning flight of robo-pigeon with quantitative stimulus parameters

IntroductionThe robo-pigeon using homing pigeons as a motion carrier has great potential in search and rescue operations due to its superior weight-bearing capacity and sustained flight capabilities. However, before deploying such robo-pigeons, it is necessary to establish a safe, stable, and long-term effective neuro-electrical stimulation interface and quantify the motion responses to various stimuli.MethodsIn this study, we investigated the effects of stimulation variables such as stimulation frequency (SF), stimulation duration (SD), and inter-stimulus interval (ISI) on the turning flight control of robo-pigeons outdoors, and evaluated the efficiency and accuracy of turning flight behavior accordingly.ResultsThe results showed that the turning angle can be significantly controlled by appropriately increasing SF and SD. Increasing ISI can significantly control the turning radius of robotic pigeons. The success rate of turning flight control decreases significantly when the stimulation parameters exceed SF > 100 Hz or SD > 5 s. Thus, the robo-pigeon's turning angle from 15 to 55° and turning radius from 25 to 135 m could be controlled in a graded manner by selecting varying stimulus variables.DiscussionThese findings can be used to optimize the stimulation strategy of robo-pigeons to achieve precise control of their turning flight behavior outdoors. The results also suggest that robo-pigeons have potential for use in search and rescue operations where precise control of flight behavior is required

The Genomes of Oryza sativa: A History of Duplications

We report improved whole-genome shotgun sequences for the genomes of indica and japonica rice, both with multimegabase contiguity, or almost 1,000-fold improvement over the drafts of 2002. Tested against a nonredundant collection of 19,079 full-length cDNAs, 97.7% of the genes are aligned, without fragmentation, to the mapped super-scaffolds of one or the other genome. We introduce a gene identification procedure for plants that does not rely on similarity to known genes to remove erroneous predictions resulting from transposable elements. Using the available EST data to adjust for residual errors in the predictions, the estimated gene count is at least 38,000–40,000. Only 2%–3% of the genes are unique to any one subspecies, comparable to the amount of sequence that might still be missing. Despite this lack of variation in gene content, there is enormous variation in the intergenic regions. At least a quarter of the two sequences could not be aligned, and where they could be aligned, single nucleotide polymorphism (SNP) rates varied from as little as 3.0 SNP/kb in the coding regions to 27.6 SNP/kb in the transposable elements. A more inclusive new approach for analyzing duplication history is introduced here. It reveals an ancient whole-genome duplication, a recent segmental duplication on Chromosomes 11 and 12, and massive ongoing individual gene duplications. We find 18 distinct pairs of duplicated segments that cover 65.7% of the genome; 17 of these pairs date back to a common time before the divergence of the grasses. More important, ongoing individual gene duplications provide a never-ending source of raw material for gene genesis and are major contributors to the differences between members of the grass family