Capturing Complex Behavior in Brain Imaging: Strategies and Instrumentation

Functional neuroimaging investigates the human brain through non-invasive recordings of brain signals or non-invasive stimulation. Traditionally, neuroimaging practitioners attempted to restrict the subject's behavior throughout the experiment to the point where it could be completely characterized by a few simple variables. Although this approach has its merits, it considerably limits the possibilities for investigating neural mechanisms underlying the organism's function under natural conditions. To overcome this limitation, researchers have increasingly focused on neuroimaging studies of subjects involved in complex ecologically-valid behavioral tasks. The shift from simple to complex behavior in neuroimaging studies brings along the demand for: (1) new instrumentation for handling the behavioral aspect of the experiment, and (2) new experimental designs that exploit the complexity of the participant's behavior instead of trying to suppress it.  The thesis comprises four publications that examine the capacity of video technology to provide new instrumentation and explore possibilities for new experimental designs utilizing rich behavioural information provided by video, in the context of magnetoencephalography (MEG) and transcranial magnetic stimulation (TMS) methods. Additionally, it introduces the Helsinki VideoMEG Project an open-source collaborative effort aimed at providing MEG practitioners with video recording and analysis tools.  The first part of the thesis (Publications I and II) examines the feasibility of augmenting TMS and MEG experiments with simultaneous synchronized video and audio recordings of the participant. The second part of the thesis (Publications III and IV) explores the possibility of using audio and video to link the participants in an MEG hyperscanning experiment simultaneous recording of MEG signals from two interacting subjects.  The results presented in this thesis demonstrate the feasibility of augmenting TMS and MEG experiments with synchronized video and audio recordings

Helsinki VideoMEG Project : Augmenting magnetoencephalography with synchronized video recordings

The primary goal of the Helsinki VideoMEG Project is to enable magnetoencephalography (MEG) practitioners to record and analyze the video of the subject during an MEG experiment jointly with the MEG data. The project provides: Hardware assembly instructions and software for setting up video and audio recordings of the participant synchronized to MEG data acquisition. Basic software tools for analyzing video and audio together with the MEG data. The resulting setup allows reliable recording of video and audio from the subject in various real-world usage scenarios. The Helsinki VideoMEG Project allowed successful establishment of video-MEG facilities in four different MEG laboratories in Finland, Sweden and the United States.Peer reviewe

Inferring Functional Brain States Using Temporal Evolution of Regularized Classifiers

We present a framework for inferring functional brain state from electrophysiological (MEG or EEG) brain signals. Our approach is adapted to the needs of functional brain imaging rather than EEG-based brain-computer interface (BCI). This choice leads to a different set of requirements, in particular to the demand for more robust inference methods and more sophisticated model validation techniques. We approach the problem from a machine learning perspective, by constructing a classifier from a set of labeled signal examples. We propose a framework that focuses on temporal evolution of regularized classifiers, with cross-validation for optimal regularization parameter at each time frame. We demonstrate the inference obtained by this method on MEG data recorded from 10 subjects in a simple visual classification experiment, and provide comparison to the classical nonregularized approach

Heat transport in a flowing complex plasma in microgravity conditions

Heat transport in a three-dimensional complex (dusty) plasma was experimentally studied in microgravity conditions using a Plasmakristall-4 (PK-4) instrument on board the International Space Station (ISS). An extended suspension of microparticles was locally heated by a shear flow created by applying the radiation pressure force of the manipulation-laser beam. Individual particle trajectories in the flow were analyzed, and from these, using a fluid heat transport equation that takes viscous heating and neutral gas drag into account, the complex plasma’s thermal diffusivity and kinematic viscosity were calculated. Their values are compared with previous results reported in ground-based experiments with complex plasmas

Biogeochemical structure of the Laptev Sea in 2015-2020 associated with the River Lena plume

The discharge of rivers and the subsequent dispersion of their plumes play a pivotal role in the biogeochemical cycling of the Arctic Ocean. Based on the data collected during annual transects conducted in the autumn period (September-October) from 2015-2020, this study explores the effect of River Lena plume dispersion on the seasonal and interannual changes in the hydrophysical and biogeochemical structure of the southeastern Laptev Sea. The temperature-salinity relationship (T-S), Redfield ratio and multiparameter cluster analysis were used to investigate variations in the water mass structure along the transect. The results revealed that the plume’s interannual and seasonal spreading patterns play a crucial role in regulating the local physical, biogeochemical, and biological processes in the southern Laptev Sea. During September-October, the hydrochemical water mass structure along the transects shifted from highly stratified to unstratified as the plume’s mixing intensity increased. Anomalous hydrochemical distributions were observed due to coastal upwelling, which was primarily characterized by high total alkalinity and nitrate levels, and low organic phosphorus, nitrite, and ammonia levels in the seawater. Wind and cold weather conditions drive deep vertical mixing of seawater, causing the resuspension of bottom sediment and the subsequent enrichment of bottom water by nutrients. Multi-parameter cluster analysis is used to describe the details of water mass structures in the highly dynamic southern Laptev Sea, with water mass structures typically undergoing significant changes within two weeks between September and October. The migration and transformation of water masses throughout the seasons are influenced by the volume of river discharge, fall-winter cooling, and atmospheric circulation patterns. Furthermore, the general atmospheric circulation is confirmed to be the primary cause of the interannual variation in the spread of the Lena River plume over the southeast Laptev Sea.publishedVersio

An Internet-Based Real-Time Audiovisual Link for Dual MEG Recordings

Hyperscanning Most neuroimaging studies of human social cognition have focused on brain activity of single subjects. More recently, "two-person neuroimaging" has been introduced, with simultaneous recordings of brain signals from two subjects involved in social interaction. These simultaneous "hyperscanning" recordings have already been carried out with a spectrum of neuroimaging modalities, such as functional magnetic resonance imaging (fMRI), electroencephalography (EEG), and functional near-infrared spectroscopy (fNIRS). Dual MEG Setup We have recently developed a setup for simultaneous magnetoencephalographic (MEG) recordings of two subjects that communicate in real time over an audio link between two geographically separated MEG laboratories. Here we present an extended version of the setup, where we have added a video connection and replaced the telephone-landline-based link with an Internet connection. Our setup enabled transmission of video and audio streams between the sites with a one-way communication latency of about 130 ms. Our software that allows reproducing the setup is publicly available. Validation We demonstrate that the audiovisual Internet-based link can mediate real-time interaction between two subjects who try to mirror each others' hand movements that they can see via the video link. All the nine pairs were able to synchronize their behavior. In addition to the video, we captured the subjects' movements with accelerometers attached to their index fingers; we determined from these signals that the average synchronization accuracy was 215 ms. In one subject pair we demonstrate inter-subject coherence patterns of the MEG signals that peak over the sensorimotor areas contralateral to the hand used in the task.Peer reviewe

Slowing of acoustic waves in electrorheological and string-fluid complex plasmas

The PK-4 laboratory consists of a direct current plasma tube into which microparticles are injected, forming a complex plasma. The microparticles acquire many electrons from the ambient plasma and are thus highly charged and interact with each other. If ion streams are present, wakes form downstream of the microparticles, which lead to an attractive term in the potential between the microparticles, triggering the appearance of microparticle strings and modifying the complex plasma into an electrorheological form. Here we report on a set of experiments on compressional waves in such a string fluid in the PK-4 laboratory during a parabolic flight and on board the International Space Station. We find a slowing of acoustic waves and hypothesize that the additional attractive interaction term leads to slower wave speeds than in complex plasmas with purely repulsive potentials. We test this hypothesis with simulations, and compare with theory

Biogeochemical structure of the Laptev Sea in 2015-2020 associated with the River Lena plume

The discharge of rivers and the subsequent dispersion of their plumes play a pivotal role in the biogeochemical cycling of the Arctic Ocean. Based on the data collected during annual transects conducted in the autumn period (September-October) from 2015-2020, this study explores the effect of River Lena plume dispersion on the seasonal and interannual changes in the hydrophysical and biogeochemical structure of the southeastern Laptev Sea. The temperature-salinity relationship (T-S), Redfield ratio and multiparameter cluster analysis were used to investigate variations in the water mass structure along the transect. The results revealed that the plume’s interannual and seasonal spreading patterns play a crucial role in regulating the local physical, biogeochemical, and biological processes in the southern Laptev Sea. During September-October, the hydrochemical water mass structure along the transects shifted from highly stratified to unstratified as the plume’s mixing intensity increased. Anomalous hydrochemical distributions were observed due to coastal upwelling, which was primarily characterized by high total alkalinity and nitrate levels, and low organic phosphorus, nitrite, and ammonia levels in the seawater. Wind and cold weather conditions drive deep vertical mixing of seawater, causing the resuspension of bottom sediment and the subsequent enrichment of bottom water by nutrients. Multi-parameter cluster analysis is used to describe the details of water mass structures in the highly dynamic southern Laptev Sea, with water mass structures typically undergoing significant changes within two weeks between September and October. The migration and transformation of water masses throughout the seasons are influenced by the volume of river discharge, fall-winter cooling, and atmospheric circulation patterns. Furthermore, the general atmospheric circulation is confirmed to be the primary cause of the interannual variation in the spread of the Lena River plume over the southeast Laptev Sea

Never Resting Brain: Simultaneous Representation of Two Alpha Related Processes in Humans

Brain activity is continuously modulated, even at “rest”. The alpha rhythm (8–12 Hz) has been known as the hallmark of the brain's idle-state. However, it is still debated if the alpha rhythm reflects synchronization in a distributed network or focal generator and whether it occurs spontaneously or is driven by a stimulus. This EEG/fMRI study aimed to explore the source of alpha modulations and their distribution in the resting brain. By serendipity, while computing the individually defined power modulations of the alpha-band, two simultaneously occurring components of these modulations were found. An ‘induced alpha’ that was correlated with the paradigm (eyes open/ eyes closed), and a ‘spontaneous alpha’ that was on-going and unrelated to the paradigm. These alpha components when used as regressors for BOLD activation revealed two segregated activation maps: the ‘induced map’ included left lateral temporal cortical regions and the hippocampus; the ‘spontaneous map’ included prefrontal cortical regions and the thalamus. Our combined fMRI/EEG approach allowed to computationally untangle two parallel patterns of alpha modulations and underpin their anatomical basis in the human brain. These findings suggest that the human alpha rhythm represents at least two simultaneously occurring processes which characterize the ‘resting brain’; one is related to expected change in sensory information, while the other is endogenous and independent of stimulus change

Factors of Deconsolidation of the Liberal Democracy Regime. The Case of the United States of America

This article analyzes the hierarchy of factors in the development of crisis trends in a consolidated democracy using the example of the United States of America. The authors assess the surge of political instability in the United States, which led to the deconsolidation of the liberal democracy regime, through the prism of centrifugal processes within the American elite and the erosion of democratic institutions over the past 30 years. The main problem of the study is the contradiction between the crisis of democratic regimes in the countries of the Euro-Atlantic region and the high indicators of the factors of the consolidation of democracy, according to classical political science theories. The authors use the path analysis method to determine the main path and hierarchy of factors of erosion of the liberal democracy regime in the United States, which is an example of the "old" democracy and, according to traditional political science, is the most protected from destructive processes. Consideration of the case of deconsolidation of the liberal democracy regime in the United States, thus, sheds light on the possible ways of democracy reversion and risk factors for stability of democratic political systems