1,121 research outputs found
Predicting bioavailability of PAHs in field-contaminated soils by passive sampling with triolein embedded cellulose acetate membranes
Triolein embedded cellulose acetate membrane (TECAM) was used for passive sampling of the fraction of naphthalene, phenanthrene, pyrene and benzo[a]pyrene in 18 field -contaminated soils. The sampling process of PAHs by TECAM fitted well with a first-order kinetics model and PAHs reached 95% of equilibrium in TECAM within 20 h. Concentrations of PAHs in TECAM (C-TECAM) correlated well with the concentrations in soils (r(2) = 0.693-0.962, p < 0.001). Furthermore. concentrations of PAHs determined in the soil solution were very close to the values estimated by C-TECAM and the partition coefficient between TECAM and water (KTECAM-W). After lipid normalization nearly 1:1 relationships were observed between PAH concentrations in TECAMs and earthworms exposed to the soils (r(2) = 0.591-0.824, n = 18, p < 0.01). These results suggest that TECAM can be a useful tool to predict bioavailability of PAHs in field-contaminated soils. (C) 2008 Elsevier Ltd. All rights reserved
Uptake and acropetal translocation of polycyclic aromatic hydrocarbons by wheat (Triticum aestivum L.) grown in field-contaminated soil
Uptake and acropetal translocation of 14 priority polycyclic aromatic hydrocarbons (PAHs) by wheat (Triticum aestivum L.) grown in 15 field-contaminated soils were investigated in a growth chamber. PAN concentrations in roots correlated positively with the corresponding concentrations in soils and negatively with the contents of soil organic carbon (p < 0.01). No clear linear relationship was found between log RCF (root concentration factor, mu g g(-1)root/mu g g(-1)soil on dry weight basis) and log K(ow) of these PAHs. Four-ring PAHs had the highest tendency to be taken up by roots. PAH concentrations in shoots correlated well with their concentrations in soils and roots. Furthermore, distribution profiles of PAHs in shoots were fairly similar to those in soils. Acropetal translocation of 10 PAHs (with log K(ow) varying from 3.45 to 5.78) was also implicated by R(t) (ratio of PAH from root-to-shoot translocation to the total accumulation in shoots) ranging from 53.6 to 72.6%. A negative linear relationship was found between log R(t) and log K(ow) of these PAHs (p < 0.01), and acropetal translocation of PAHs depended on their chemical properties
DEM simulation of the mechanical properties of SiC ceramic under pre-stressing
In this paper, the method of discrete element model (DEM) simulation was used to investigate the mechanical properties of SiC ceramic materials under the action of pre-stress. Using the bonded particle model (BPM), several different numerical tests (such as UCT, TPB, SENB tests) of SiC ceramic were established. Different pre-stress values were applied on the lateral surface of the ceramic specimen during the numerical simulation process, all tests were carried out at least 5 times with different random number, and the average mechanical properties results were calculated. It was showed that the existence of pre-stress has a significant effect on the mechanical properties of materials. It can enhance the strength of materials, while the force action on material in machining process force or action force the crackβs initiation and propagation was limited
Identification of a candidate gene for Rc-D1, a locus controlling red coleoptile colour in wheat
Red coleoptile is an easily observed agronomic trait of wheat and has been extensively studied. However, the molecular mechanism of this trait has not yet been revealed. In this study, the MYB gene TaMYB-D1 was isolated from the wheat cultivar βGy115β, which possesses red coleoptiles. This gene resided at the short arm of the homoelogous group 7 chromosomes. TaMYB-D1 was the only gene expressed in the coleoptiles of βGy115β and was not expressed in βOpataβ and βCSβ, which have uncoloured coleoptiles. Phylogenetic analysis placed TaMYB-D1 very close to ZmC1 and other MYB proteins regulating anthocyanin biosynthesis. The encoded protein of TaMYB-D1 had an integrated DNA binding domain of 102 amino acids and a transcription domain with 42 amino acids, similar to the structure of ZmC1. Transient expression analysis in onion epidermal cells showed that TaMYB-D1 was located at the plant nucleus, which suggested its role as a transcription factor. The expression of TaMYB-D1 was accompanied with the expression of TaDFR and anthocyanin biosynthesis in the development of the coleoptile of βGy115β. Transient expression analysis showed that only TaMYB-D1 induced a few βOpataβ coleoptile cells to synthesize anthocyanin in light, and the gene also induced a colour change to red in many cells with the help of ZmR. All of these results suggested TaMYB-D1 as the candidate gene for the red coleoptile trait of βGy115β
Charge-order melting and magnetic phase separation in thin films of Pr0.7Ca0.3MnO3
Quantum Matter and Optic
First-order nature of a metamagnetic transition and mechanism of giant magnetoresistance in Mn2Sb0.95Sn0.05
Quantum Matter and Optic
Plasmid encoding matrix protein of vesicular stomatitis viruses as an antitumor agent inhibiting rat glioma growth in situ
Aim: Oncolytic effect of vesicular stomatitis virus (VSV) has been proved previously. Aim of the study is to investigate glioma inhibition effect of Matrix (M) protein of VSV in situ. Materials and Methods: A recombinant plasmid encoding VSV M protein (PM) was genetically engineered, and then transfected into cultured C6 gliomas cells in vitro. C6 transfected with Liposome-encapsulated PM (LEPM) was implanted intracranially for tumorigenicity study. In treatment experiment, rats were sequentially established intracranial gliomas with wild-typed C6 cells, and accepted LEPM injection intravenously. Possible mechanism of M protein was studied by using Hoechst staining, PI-stained flow cytometric analysis, TUNEL staining and CD31 staining. Results: M protein can induce generous gliomas lysis in vitro. None of the rats implanted with LEPM-treated cells developed any significant tumors, whereas all rats in control group developed tumors. In treatment experiment, smaller tumor volume and prolonged survival time was found in the LEPM-treated group. Histological studies revealed that possible mechanism were apoptosis and anti-angiogenesis. Conclusion: VSV-M protein can inhibit gliomas growth in vitro and in situ, which indicates such a potential novel biotherapeutic strategy for glioma treatment.Π¦Π΅Π»Ρ: ΠΈΠ·ΡΡΠΈΡΡ ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΡ ΠΌΠ°ΡΡΠΈΠΊΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΠΈΠ½Π° (Π ΠΏΡΠΎΡΠ΅ΠΈΠ½Π°) Π²ΠΈΡΡΡΠ° Π²Π΅Π·ΠΈΠΊΡΠ»ΡΡΠ½ΠΎΠ³ΠΎ ΡΡΠΎΠΌΠ°ΡΠΈΡΠ° (ΠΠΠ‘) ΡΠ³Π½Π΅ΡΠ°ΡΡ ΡΠΎΡΡ Π³Π»ΠΈΠΎΠΌΡ
in situ. ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ: ΡΠΊΠΎΠ½ΡΡΡΡΠΈΡΠΎΠ²Π°Π½Π° ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½ΡΠ½Π°Ρ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Π°, ΠΊΠΎΠ΄ΠΈΡΡΡΡΠ°Ρ Π ΠΏΡΠΎΡΠ΅ΠΈΠ½ ΠΠΠ‘, ΠΊΠΎΡΠΎΡΠ°Ρ Π·Π°ΡΠ΅ΠΌ Π±ΡΠ»Π°
ΡΡΠ°Π½ΡΡΠ΅ΡΠΈΡΠΎΠ²Π°Π½Π° Π² ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ Π³Π»ΠΈΠΎΠΌΡ Π‘6 in. ΠΠ»Π΅ΡΠΊΠΈ Π³Π»ΠΈΠΎΠΌΡ Π‘6, ΡΡΠ°Π½ΡΡΠ΅ΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΈΠ½ΠΊΠ°ΠΏΡΡΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠΌ
Π² Π»ΠΈΠΏΠΎΡΠΎΠΌΡ Π ΠΏΡΠΎΡΠ΅ΠΈΠ½ΠΎΠΌ (ΠΠΠΠ), ΠΈΠΌΠΏΠ»Π°Π½ΡΠΈΡΠΎΠ²Π°Π»ΠΈ ΠΈΠ½ΡΡΠ°ΠΊΡΠ°Π½ΠΈΠ°Π»ΡΠ½ΠΎ Π΄Π»Ρ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ ΡΡΠΌΠΎΡΠΎΠ³Π΅Π½Π½ΠΎΡΡΠΈ. Π ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ΅ ΠΊΡΡΡΠ°ΠΌ
Ρ ΡΡΠ°Π½ΡΠΏΠ»Π°Π½ΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΈΠ½ΡΡΠ°ΠΊΡΠ°Π½ΠΈΠ°Π»ΡΠ½ΠΎ Π³Π»ΠΈΠΎΠΌΠΎΠΉ Π‘6 (ΠΈΡΡ
ΠΎΠ΄Π½ΡΠΉ ΡΡΠ°ΠΌΠΌ) Π²Π½ΡΡΡΠΈΠ²Π΅Π½Π½ΠΎ Π²Π²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΠΠΠ. ΠΠΏΠΎΠΏΡΠΎΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅
Π ΠΏΡΠΎΡΠ΅ΠΈΠ½Π° Π½Π° ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ ΠΈΠ·ΡΡΠ°Π»ΠΈ Ρ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ ΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠ΅Π½ΡΠ½ΠΎΠΉ ΠΌΠΈΠΊΡΠΎΡΠΊΠΎΠΏΠΈΠΈ (ΠΎΠΊΡΠ°ΡΠΈΠ²Π°Π½ΠΈΠ΅ ΠΏΠΎ Π₯Π΅Ρ
ΡΡΡ),
ΠΏΡΠΎΡΠΎΡΠ½ΠΎΠΉ ΡΠΈΡΠΎΠΌΠ΅ΡΡΠΈΠΈ (ΠΎΠΊΡΠ°ΡΠΈΠ²Π°Π½ΠΈΠ΅ ΠΏΡΠΎΠΏΠΈΠ΄ΠΈΡΠΌΠΎΠΌ ΠΉΠΎΠ΄ΠΈΠ΄ΠΎΠΌ), TUNEL Π²Π°ΡΠΊΡΠ»ΡΡΠΈΠ·Π°ΡΠΈΡ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈ Π³ΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈ ΠΈ Π²Π°ΡΠΊΡΠ»ΡΡΠΈΠ·Π°ΡΠΈΡ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈ Π³ΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈ ΠΈ
ΠΈΠΌΠΌΡΠ½ΠΎΠ³ΠΈΡΡΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈ Ρ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ Π°Π½ΡΠΈ-CD31 ΠΌΠΎΠ½ΠΎΠΊΠ»ΠΎΠ½Π°Π»ΡΠ½ΡΡ
Π°Π½ΡΠΈΡΠ΅Π». 31 ΠΌΠΎΠ½ΠΎΠΊΠ»ΠΎΠ½Π°Π»ΡΠ½ΡΡ
Π°Π½ΡΠΈΡΠ΅Π». 31 ΠΌΠΎΠ½ΠΎΠΊΠ»ΠΎΠ½Π°Π»ΡΠ½ΡΡ
Π°Π½ΡΠΈΡΠ΅Π». Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ: Π ΠΏΡΠΎΡΠ΅ΠΈΠ½ ΠΌΠΎΠΆΠ΅Ρ ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°ΡΡ
Π»ΠΈΠ·ΠΈΡ ΠΊΠ»Π΅ΡΠΎΠΊ Π³Π»ΠΈΠΎΠΌΡ in. ΠΠΈ Ρ ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΠΆΠΈΠ²ΠΎΡΠ½ΠΎΠ³ΠΎ Ρ ΡΡΠ°Π½ΡΠΏΠ»Π°Π½ΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠΌΠΈ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ Π³Π»ΠΈΠΎΠΌΡ, ΠΎΠ±ΡΠ°Π±ΠΎΡΠ°Π½Π½ΡΠΌΠΈ ΠΠΠΠ,
Π½Π΅ Π²ΠΎΠ·Π½ΠΈΠΊΠ°Π»ΠΈ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠ°Π·ΠΌΠ΅ΡΠ°, ΡΠΎΠ³Π΄Π° ΠΊΠ°ΠΊ Ρ Π²ΡΠ΅Ρ
ΠΊΡΡΡ ΠΈΠ· ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΡ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΡΠ°Π·Π²ΠΈΠ²Π°Π»ΠΈΡΡ. Π Π³ΡΡΠΏΠΏΠ΅
ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
, ΠΊΠΎΡΠΎΡΡΠΌ Π²Π²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΠΠΠ, ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ Π±ΡΠ»ΠΈ ΠΌΠ΅Π½ΡΡΠ΅Π³ΠΎ ΠΎΠ±ΡΠ΅ΠΌΠ° ΠΈ ΠΎΡΠΌΠ΅ΡΠ°Π»ΠΈ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΠΏΡΠΎΠ΄ΠΎΠ»ΠΆΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ ΠΆΠΈΠ·Π½ΠΈ
ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Π ΠΏΡΠΎΡΠ΅ΠΈΠ½ ΠΏΡΠΎΡΠ²Π»ΡΠ΅Ρ Π°Π½ΡΠΈΠ°Π½Π³ΠΈΠΎΠ³Π΅Π½Π½ΡΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π° ΠΈ ΠΎΠ±Π»Π°Π΄Π°Π΅Ρ ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΡΡ ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°ΡΡ Π°ΠΏΠΎΠΏΡΠΎΠ·.
ΠΡΠ²ΠΎΠ΄Ρ: Π ΠΏΡΠΎΡΠ΅ΠΈΠ½ ΠΠΠ‘ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΡΠ΅Ρ ΡΠΎΡΡ Π³Π»ΠΈΠΎΠΌΡ in ΠΈ in. ΠΠ° ΡΡΠΎΠΉ ΠΎΡΠ½ΠΎΠ²Π΅ ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π° ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΠΎ Π½ΠΎΠ²Π°Ρ
Π±ΠΈΠΎΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΡΡΠ°ΡΠ΅Π³ΠΈΡ Π΄Π»Ρ Π»Π΅ΡΠ΅Π½ΠΈΡ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Π³Π»ΠΈΠΎΠΌΠ°ΠΌΠΈ
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