The study of initial permeability temperature dependences for LiTiZn ferrite ceramics

Results of obtaining and analyzing the temperature dependences of initial permeability of ferrite ceramics are presented in the paper. It was shown that the level of the defective state of ferrite ceramics can be obtained from the value of two parameters [alpha] and [beta] of the phenomenological expression describing the experimental dependences. The results showed that the main criterion of the defect state is the parameter [beta]/[alpha], which is related to the elastic stresses in the material. An indicator of the structure perfection is also the value of the maximum of the initial permeability near the Curie temperature

Development of the cell-ELISA test for the subtype identification of circulating influenza A(H1) and A(H3) viruses

The sensitive version of cell-ELISA was developed for the subtype-specific differentiation of current influenza A(H1N1)pdm09 and A(H3N2) viruses that are circulating in the human population. This method is based on the estimation of virus reproduction in infected MDCK cells. The detection step of this method is an interaction of the subtype-specific monoclonal antibodies (mAbs) with the viral hemagglutinin (НА) molecule. The influenza A virus strains, isolated in the 2014 epidemic season, were used to validate this method.It was shown that when using mAbs # 1/ # 2 or # 4 at a concentration of 10-15 µg/ml, the developed variant of cell-ELISA was able to detect НА protein synthesized in the infected cells of influenza A(H3N2) and A(H1N1)pdm09 viruses, respectively.The developed method can be used for the identification of modern influenza A viruses with low hemagglutination activity, which is not possible by the conventional hemagglutination inhibition test.The sensitive version of cell-ELISA was developed for the subtype-specific differentiation of current influenza A(H1N1)pdm09 and A(H3N2) viruses that are circulating in the human population. This method is based on the estimation of virus reproduction in infected MDCK cells. The detection step of this method is an interaction of the subtype-specific monoclonal antibodies (mAbs) with the viral hemagglutinin (НА) molecule. The influenza A virus strains, isolated in the 2014 epidemic season, were used to validate this method. It was shown that when using mAbs # 1/ # 2 or # 4 at a concentration of 10-15 µg/ml, the developed variant of cell-ELISA was able to detect НА protein synthesized in the infected cells of influenza A(H3N2) and A(H1N1)pdm09 viruses, respectively. The developed method can be used for the identification of modern influenza A viruses with low hemagglutination activity, which is not possible by the conventional hemagglutination inhibition test

Protoplasmic Astrocytes Enhance the Ability of Neural Stem Cells to Differentiate into Neurons In Vitro

Protoplasmic astrocytes have been reported to exhibit neuroprotective effects on neurons, but there has been no direct evidence for a functional relationship between protoplasmic astrocytes and neural stem cells (NSCs). In this study, we examined neuronal differentiation of NSCs induced by protoplasmic astrocytes in a co-culture model. Protoplasmic astrocytes were isolated from new-born and NSCs from the E13-15 cortex of rats respectively. The differentiated cells labeled with neuron-specific marker β-tubulin III, were dramatically increased at 7 days in the co-culture condition. Blocking the effects of brain-derived neurotrophic factor (BDNF) with an anti-BDNF antibody reduced the number of neurons differentiated from NSCs when co-cultured with protoplasmic astrocytes. In fact, the content of BDNF in the supernatant obtained from protoplasmic astrocytes and NSCs co-culture media was significantly greater than that from control media conditions. These results indicate that protoplasmic astrocytes promote neuronal differentiation of NSCs, which is driven, at least in part, by BDNF

Использование микрокультурального иммуноферментного анализа для субтиповой идентификации циркулирующих вирусов гриппа А(Н1) и А(Н3)

The sensitive version of cell-ELISA was developed for the subtype-specific differentiation of current influenza A(H1N1)pdm09 and A(H3N2) viruses that are circulating in the human population. This method is based on the estimation of virus reproduction in the infected MDCK cells. The detection step of this method is an interaction of the subtype-specific monoclonal antibodies (mAbs) with the viral hemagglutinin (НА). The influenza A virus strains, isolated in 2014 epidemic season, were used to validate this method. It was shown that by using mAb # 1/ # 2 or # 4 at a concentration of 10-15 µg / ml the developed variant of cell-ELISA allows the detection of НА protein, synthesized in the cells infected with influenza A(H3N2) or A(H1N1)pdm09 virus, respectively. The developed method can be used for identification of HA subtype of modern influenza A viruses with low HA activity, which is not possible by the conventional hemagglutination inhibition test.Разработан чувствительный вариант микрокультурального ИФА (cell-ELISA) для субтиповой дифференциации современных вирусов гриппа А(Н1N1)pdm09 и А(Н3N2), циркулирующих в человеческой популяции. Метод основан на оценке репродукции вируса в инфицированной культуре клеток MDCK c использованием на стадии детекции cубтип-специфичных моноклональных антител (mAb), взаимодействующих с гемагглютинином (HA) вируса гриппа. При отработке метода использованы штаммы вирусов гриппа A, выделенные из клинических образцов в эпидемический сезон 2014 года. Показано, что при использовании mAb #1/#2 или #4 в концентрации 10–15 мкг/мл разработанный вариант cell-ELISA позволяет детектировать синтезируемый в зараженных клетках белок HA соответственно вирусов гриппа А(H3N2) или A(H1N1)pdm09. Разработанный метод может быть использован для идентификации современных штаммов вируса гриппа А в процессе их репродукции в клеточной культуре MDCK, что позволяет проводить субтипирование вирусов с низкой гемагглютинирующей активностью, когда невозможно использовать общепринятый метод – реакцию торможения гемагглютинации (РТГА)

Дифференциальная диагностика токсического эпидермального некролиза (синдрома Лайелла) в ОРИТ: клинические наблюдения

Toxic epidermal necrolysis (TEN) is a critical life-threating condition developing as the total detachment of epidermidis and characterized by severe pathological reactions of all body systems. The current article describes two cases of TEN with similar clinical and laboratory signs. In one case the diagnosis of TEN was subsequently refused.The objective: analysis of methods of clinical and differential diagnostics of conditions accompanied with massive epidermidis detachment in ICU patients.Results. The immunomorphological evaluation of skin specimen obtained from the patient with a torpid form of TEN showed linear IgG fixation in the intercellular space of stratum basale, stratum spinosum and stratum granulosum and C3 fixation in the intercellular space of stratum basale.Conclusion. The complex of anamnesis data and pathomorphological evaluation of skin are crucial for the diagnosis and treatment of patients with atypical TEN.Токсический эпидермальный некролиз (ТЭН) – критическое жизнеугрожающее состояние, развивающееся в виде тотального отслоения эпидермиса и характеризующееся тяжелыми реакциями со стороны всех систем организма. Приведено описание двух клинических случаев со сходной клинико-лабораторной симптоматикой, в одном из которых диагноз ТЭН впоследствии был опровергнут.Цель: анализ методов клинической и лабораторной дифференциальной диагностики состояний, сопровождающихся массивной отслойкой эпидермиса, у пациентов, пребывающих в ОРИТ.Результаты. При иммуноморфологическом исследовании биоптата кожи, полученного от пациентки с торпидным течением ТЭН, обнаружена четкая линейная фиксация IgG в межклеточной связывающей субстанции (МСС) базального, шиповатого и зернистого слоев эпидермиса, а также фиксация С3-компонента комплемента в МСС базального слоя эпидермиса.Заключение. Совокупность анамнестических сведений и результатов патоморфологического исследования методом прямой иммунофлюоресценции имеет критически важное значение для установления диагноза и выбора лечебной тактики у пациентов с атипичным течением ТЭН

A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)

Meeting abstrac

Clinical features of post-COVID-19 period. Results of the international register “Dynamic analysis of comorbidities in SARS-CoV-2 survivors (AKTIV SARS-CoV-2)”. Data from 6-month follow-up

Aim. To study the clinical course specifics of coronavirus disease 2019 (COVID-19) and comorbid conditions in COVID-19 survivors 3, 6, 12 months after recovery in the Eurasian region according to the AKTIV register. Material and methods.The AKTIV register was created at the initiative of the Eurasian Association of Therapists. The AKTIV register is divided into 2 parts: AKTIV 1 and AKTIV 2. The AKTIV 1 register currently includes 6300 patients, while in AKTIV 2 — 2770. Patients diagnosed with COVID-19 receiving in- and outpatient treatment have been anonymously included on the registry. The following 7 countries participated in the register: Russian Federation, Republic of Armenia, Republic of Belarus, Republic of Kazakhstan, Kyrgyz Republic, Republic of Moldova, Republic of Uzbekistan. This closed multicenter register with two nonoverlapping branches (in- and outpatient branch) provides 6 visits: 3 in-person visits during the acute period and 3 telephone calls after 3, 6, 12 months. Subject recruitment lasted from June 29, 2020 to October 29, 2020. Register will end on October 29, 2022. A total of 9 fragmentary analyzes of the registry data are planned. This fragment of the study presents the results of the post-hospitalization period in COVID-19 survivors after 3 and 6 months. Results. According to the AKTIV register, patients after COVID-19 are characterized by long-term persistent symptoms and frequent seeking for unscheduled medical care, including rehospitalizations. The most common causes of unplanned medical care are uncontrolled hypertension (HTN) and chronic coronary artery disease (CAD) and/or decompensated type 2 diabetes (T2D). During 3- and 6-month follow-up after hospitalization, 5,6% and 6,4% of patients were diagnosed with other diseases, which were more often presented by HTN, T2D, and CAD. The mortality rate of patients in the post-hospitalization period was 1,9% in the first 3 months and 0,2% for 4-6 months. The highest mortality rate was observed in the first 3 months in the group of patients with class II-IV heart failure, as well as in patients with cardiovascular diseases and cancer. In the pattern of death causes in the post-hospitalization period, following cardiovascular causes prevailed (31,8%): acute coronary syndrome, stroke, acute heart failure. Conclusion. According to the AKTIV register, the health status of patients after COVID-19 in a serious challenge for healthcare system, which requires planning adequate health system capacity to provide care to patients with COVID-19 in both acute and post-hospitalization period

Технологічні аспекти сушіння коренеплодів пастернаку

Purpose. The purpose of this study was to improve the technology of obtaining dried parsnip for further use in the food industry.Methods. Drying parsnip was carried out by the convection method using clean air (moisture content 10 g/kg) at a heat carrier temperature of 60 °С and an airflow rate of 2.5 m/s. The structural changes occurred in dried parsnip root under the effect of temperature were studied using the differential scanning calorimeter QDSC-20 Thermo Fisher SCIENTIFIC.Results. The article presents research results on the technology of obtaining parsnip root powder from parsnip roots. The technology includes cleaning and crushing roots, drying the mass by the convection method, packing and storage of the powder. The process of convection drying of crushed parsnip roots was explored and the optimum parameters of processing were determined. Structural changes occurring in the roots during drying were studied.Conclusions. The optimal parameters of convective drying of crushed parsnip roots are the following: the particle size 4×2×1 mm, the temperature of 60 °С and the airflow rate of 2.5 m/s. The main temperature points of relieving hygroscopic and bound water (60–168 °С), partial melting of sugars (176–188.5 °С), complete melting of sugars and ash formation (205–225 °С) were determined. The most significant accumulation of decomposition products occurs at a temperature of 192 °C. A process chart for the production of powder from parsnips ensuring the yield of dried parsnip from 23 to 24% was developed.Цель. Усовершенствование технологии получения пастернака сушеного для дальнейшего использования в пищевой промышленности.Методы. Сушку пастернака проводили путём использования чистого воздуха (d = 10 г/кг сухого воздуха) конвективным способом при температуре теплоносителя 60 °С и скорости его движения 2,5 м/с. Структурные изменения, которые происходили в веществах корнеплода пастернака сушеного под действием температуры, изучали с использованием дифференциального сканирующего калориметра QDSC-20 Termo Fisher SCIENTIFIC.Результаты. Представлено результаты исследований по технологии получения порошка с корнеплодов пастернака, которая включает: очистку и нарезку корнеплодов, их сушку конвективным способом, упаковки и хранения порошка. Исследован процесс конвективной сушки измельченных корнеплодов пастернака и определен оптимальный режим для промышленного производства. Изучены структурные изменения, происходящие в корнеплодах во время сушки.Выводы. Установлено, что оптимальным режимом конвективной сушки измельченных корнеплодов пастернака являются: размер частиц 4×2×1 мм, температура 60 °С и скорости движения теплоносителя 2,5 м/с. Определены основные температурные точки, в которых удаляется гигроскопическая и связанная вода (60–168 °С), частичного плавления сахаров (176–188,5 °С), полное плавления сахара и образования золы (205–225 °С). Показано, что при температуре 192 °С происходит наибольшее накопление продуктов распада соединений. Разработана технологическая схема получения порошка из корнеплодов пастернака и определено, что выход пастернака сушеного составляет 23–24 %.Мета. Удосконалити технологію отримання пастернаку сушеного для подальшого використання в харчовій промисловості.Методи. Сушіння пастернаку здійснювали чистим повітрям (d = 10 г/кг сухого повітря) конвективним способом за температури теплоносія 60 °С і швидкості його руху 2,5 м/с. Структурні зміни, які відбувались у речовинах коренеплоду пастернаку сушеному під дією температури, вивчали із застосуванням диференційного сканувального калориметра QDSC-20 Termo Fisher SCIENTIFIC.Результати. Представлено результати досліджень щодо технології отримання порошку з коренеплодів пастернаку, яка включає очищення та подрібнення коренеплодів, їхнє сушіння конвективним способом, пакування та зберігання порошку. Досліджено процес конвективного сушіння подрібнених коренеплодів пастернаку та визначено оптимальний режим для промислового виробництва. Вивчено структурні зміни, що відбуваються в коренеплодах під час сушіння.Висновки. Установлено, що оптимальним режимом конвективного сушіння подрібнених коренеплодів пастернаку є: розмір часток 4×2×1 мм, температура 60 °С і швидкість руху теплоносія 2,5 м/с. Визначено основні температурні точки, за яких видаляється гігроскопічна і зв’язана вода (60–168 °С), часткового плавлення цукрів (176–188,5 °С), повне плавлення цукрів та утворення золи (205–225 °С). Показано, що за температури 192 °С відбувається найбільше накопичення продуктів розкладу сполук. Розроблено технологічну схему отримання порошку з коренеплодів пастернаку та визначено, що вихід пастернаку сушеного становить 23–24 %

Intensifying drying process with creation of functional plant compositions

Introduction. The process of drying agricultural raw products is associated with loss of bioactive substances by the products exposed to heat, light, oxygen, or рН medium. It is reasonable to enhance the table beet processing technology in order to achieve maximum betanin conservation at lower energy consumption. Materials and methods. Table beets, rhubarbs, lemons, and tomatoes were dried at temperature of 50 to 100 ºС, air speed of 1.5 to 3.5 m/s, heat carrier water content of 7 to 15 g/kg, and layer thickness of 2 to 20 mm. The betanіn content was determined via absorption spectra, using the optical density value at 540 nm wavelength. A differential microcalorimeter was used for measuring evaporation heat consumption. Results and discussion.The effect of raw product pre-drying preparation was studied. With no preliminary preparation, the loss of betanin after drying reaches 66 %. The preliminary preparation technology we have developed includes boiling whole root crops with optimal selection of acid medium and allows us to reduce the betanin loss down to 6 %. Regretfully, the process requires large energy consumption. Low energy consumption pre-drying preparation method was developed for antioxidant raw products with thermal processing replaced by blending. The betanin loss, in this case, does not exceed 5 %. Optimal drying temperature of betanin-containing raw stock, after its preliminary processing, is 60 °С. It allows to keep up to 95 % of betanіn. Specific heat consumption for water evaporation out of the developed table beet based antioxidant plant compositions, with addition of rhubarb and lemon, is less by 4 to 5 % as compared to the initial components. Conclusions. Dependence of betanin loss in plant raw stock on the material temperature and composition components, in the course of their pre-drying preparation, was found. It was also found that water evaporation heat, for some antioxidant plant compositions developed, is less as compared to the initial raw stock components. It is reasonable to use the results for development of industrial thermal processing technologies used for functional food powder production