The role of bed-penetrating Kelvin–Helmholtz vortices on local and instantaneous bedload sediment transport

We present results from open-channel, Euler–Lagrange (EL) simulations of turbulent flow over an erodible particle bed at a shear Reynolds number of Reτ=180 . Upon space and time averaging, our simulations correctly reproduce the Wong & Parker (ASCE J. Hydraul. Engng, vol. 132, issue 11, 2006, pp. 1159–1168) bedload transport relation (WP). However, local and instantaneous sediment flux shows orders of magnitude scatter around the WP prediction. Visualization of the vortical structures using swirling strength shows the existence of bed-penetrating Kelvin–Helmholtz (KH) vortex packets, which coupled with particle inertia are primarily responsible for the large scatter. The results also show that Euler–Euler (EE) simulations, where the individual sediment grains are not distinguished, are still able to reliably capture the turbulent shear stress variation, however, they do not capture the wide distribution of sediment flux indicative of saltating transport. The KH vortices induce non-zero streamwise and bed-normal velocities at the upper surface of the bed, which must be considered in EE simulations

Front dynamics of elliptical gravity currents on a uniform slope

In the present investigation, we report data from direct numerical simulations of elliptical, finite release, Boussinesq gravity currents propagating down a uniform slope. The study comprises a series of simulations of elliptical gravity currents on a range of slope angles. The shape parameters are varied to study the effects of the initial cross-sectional aspect ratio (Λ0) and mean height to lock radius ratio (Γ) on the dynamics of the gravity current. It is found that the long-time development of the current spatial mass distribution is influenced by its initial shape at smaller slope angles (θ=5∘ and 10∘) whereas the long-time motion of the gravity current is relatively insensitive to its initial shape but is sensitive to the slope angle. The switching of axes are observed for all the noncircular releases studied in the present work. Multiple acceleration phases are observed for the current center of mass in the case of the current with a small or moderate initial cross-sectional aspect ratio (Λ0=0.1, 0.2, 0.5, 1, and 2) whereas one single acceleration phase exists for the current with a large initial cross-sectional aspect ratio (Λ0=5 and 10). The Froude numbers (Fr) for the currents released with the same slope angle but different initial shapes are observed to attain a similar constant value after the second acceleration phase. The mean Froude number (¯¯¯Fr) is seen to increase with increasing slope angles. The mean height to lock radius ratio is found to affect only the early development of the current with little influence on the long-time evolution

Investigation of theoretical scaling laws using large eddy simulations for airborne spreading of viral contagion from sneezing and coughing

Using a set of large eddy point-particle simulations, we explore the fluid dynamics of an ejected puff resulting from a cough/sneeze. The ejection contains over 61 000 potentially virus-laden droplets at an injection Reynolds number of about 46 000, comparable to an actual cough/sneeze. We observe that global puff properties, such as centroid, puff volume, momentum, and buoyancy vary little across realizations. Other properties, such as maximum extent, shape, and edge velocity of the puff, may exhibit substantial variation. In many realizations, a portion of the puff splits off and advances along a random direction, while keeping airborne droplet nuclei afloat. This peeled-off portion provides a mechanism for virus-laden droplets to travel over large distances in a short amount of time. We also observe that the vast majority of droplets remain suspended within the puff after all liquid has evaporated. The main objectives of the study are to (i) evaluate assumptions of Balachandar\u27s et al. theory [Int. J. Multiphase Flow 132, 103439 (2020)], which include buoyancy effects, shape of the puff, and droplet evaporation rate, (ii) obtain values of closure parameters, which include location and time of the virtual origin, and puff entrainment and drag coefficients, and (iii) evaluate the accuracy of the theory in predicting the shape, size, and location of the puff, as well as droplet number density long after ejection. The theory adequately predicts global puff properties including size, velocity, and distance traveled, the largest size of droplets that exit the puff due to settling, and the droplet size distribution within the puff long after ejection

Direct Numerical Simulation of Transverse Ripples: 2. Self-Similarity, Bedform Coarsening, and Effect of Neighboring Structures

Coupled bed-flow direct numerical simulations investigating the early stages of pattern formation and bedform (ripple) interactions were examined in a previous paper (Part 1), making use of the resolved flow field. In this paper (Part 2), we compare our results to published experimental data and provide an extensive quantitative analysis of the bed using spectral analysis and two-point correlations. The effect of the mobile rippled bed on the flow structure and turbulence is investigated locally (at specific streamwise locations) and over the entire computational domain. We show that developing ripples attain a self-similar profile in both the shape and the corresponding bed shear stress. We demonstrate the importance of neighboring structures, especially upstream neighbors, on bedform dynamics in terms of the growth, decay, and speed of ripples. Finally, we examine the defect-free interactions in the later stages of bed evolution, which primarily lead to wave coarsening. Key Points Isolated ripples maintain a self-similar shape and bed shear stress profiles Bedform-bedform interactions can significantly modify bedform celerity Spectra of bed height variation suggest a Reynolds number dependenc

Direct Numerical Simulation of Transverse Ripples: 1. Pattern Initiation and Bedform Interactions

We present results of coupled direct numerical simulations between flow and a deformable bed in a horizontally periodic, turbulent open channel at a shear Reynolds number of Reτ = 180. The feedback between the temporally and spatially evolving bed and the flow is enforced via the immersed boundary method. Using the near-bed flow field, we provide evidence on the role of locally intense near-bed vortical structures during the early stages of bed formation, from the emergence of quasi-streamwise streaks to the formation of incipient bedform crestlines. Additionally, we take a new look at a number of defect-related bedform interactions, including lateral linking, defect and bedform repulsion, merging, and defect creation, and show that the underlying mechanisms, in these flow-aligned interactions, are very similar to each other. Consequently, the interactions are labeled differently depending on the geometry of interacting structures and the outcome of the interaction. In the companion paper, we compare our results to published experimental data and provide an extensive quantitative analysis of the bed, where we demonstrate the importance of neighboring structures, especially upstream neighbors, on bedform dynamics (growth/decay and speed) and wave coarsening. Video files of bed evolution are available in the supporting information. Key Points Mesoscale resolved simulations show the different mechanisms for bedform-bedform interactions to be very similar to each other Similar to laminar flows over dunes and ripples, a positive phase shift is observed between bed shear stress and topology even in mesoscale-resolved turbulent flow field Simulations match Coleman and Melville (1996) theory on bedform initiation from a flat be

Raman studies of Ge-promoted stress modulation in 3C-SiC grown on Si(111)

We present a study of the stress state in cubic silicon carbide (3C-SiC) thin films (120 and 300 nm) grown by solid-source molecular-beam epitaxy (SSMBE) on Si(111) substrates modified by the deposition of germanium prior to the carbonization of Si. μ -Raman measurements were used to determine the residual stress existing in the 3C-SiC layers. The stress is found to decrease linearly with increasing Ge quantity but with different strength depending on the 3C-SiC thickness deposited after the introduction of Ge. Based on secondary ions mass spectroscopy (SIMS) and transmission electron microscopy (TEM) analyses it is suggested that the Ge introduced prior to the carbonization step remains in the near-interface region and reduces the Si outdiffusion, which further reduces the stress state of the 3C-SiC layers

Ge-modified Si(100) substrates for the growth of 3C-SiC (100)

An alternative route to improve the epitaxial growth of 3C-SiC (100) on Si(100) was developed. It consists in covering the silicon wafers with germanium prior to the carbonization step of the silicon substrate. Transmission electron microscopy and μ -Raman investigations revealed an improvement in the residual strain and crystalline quality of the grown 3C-SiC layers comparable to or better than in the case of 3C-SiC grown on silicon on insulator substrates. These beneficial effects were reached by using a Ge coverage in the range of 0.5-1 monolayer

Microcollimator for Micrometer-Wide Stripe Irradiation of Cells Using 20–30 keV X Rays

The exposure of subnuclear compartments of cells to ionizing radiation is currently not trivial. We describe here a collimator for micrometer-wide stripe irradiation designed to work with conventional high-voltage X-ray tubes and cells cultured on standard glass cover slips. The microcollimator was fabricated by high-precision silicon micromachining and consists of X-ray absorbing chips with grooves of highly controlled depths, between 0.5-10 mum, along their surfaces. These grooves form X-ray collimating slits when the chips are stacked against each other. The use of this device for radiation biology was examined by irradiating human cells with X rays having energies between 20-30 keV. After irradiation, p53 binding protein 1 (53BP1), a nuclear protein that is recruited at sites of DNA double-strand breaks, clustered in lines corresponding to the irradiated stripes

Turmeric and Its Major Compound Curcumin on Health: Bioactive Effects and Safety Profiles for Food, Pharmaceutical, Biotechnological and Medicinal Applications

Curcumin, a yellow polyphenolic pigment from the Curcuma longa L. (turmeric) rhizome, has been used for centuries for culinary and food coloring purposes, and as an ingredient for various medicinal preparations, widely used in Ayurveda and Chinese medicine. In recent decades, their biological activities have been extensively studied. Thus, this review aims to offer an in-depth discussion of curcumin applications for food and biotechnological industries, and on health promotion and disease prevention, with particular emphasis on its antioxidant, anti-inflammatory, neuroprotective, anticancer, hepatoprotective, and cardioprotective effects. Bioavailability, bioefficacy and safety features, side effects, and quality parameters of curcumin are also addressed. Finally, curcumin’s multidimensional applications, food attractiveness optimization, agro-industrial procedures to offset its instability and low bioavailability, health concerns, and upcoming strategies for clinical application are also covered

S-Glutathionylation at Cys328 and Cys542 Impairs STAT3 Phosphorylation.

STAT3 is a latent transcription factor that promotes cell survival and proliferation and is often constitutively active in cancers. Although many reports provide evidence that STAT3 is a direct target of oxidative stress, its redox regulation is poorly understood. Under oxidative conditions STAT3 activity can be modulated by S-glutathionylation, a reversible redox modification of cysteine residues. This suggests the possible cross-talk between phosphorylation and glutathionylation and points out that STAT3 is susceptible to redox regulation. Recently, we reported that decreasing the GSH content in different cell lines induces inhibition of STAT3 activity through the reversible oxidation of thiol groups. In the present work, we demonstrate that GSH/diamide treatment induces S-glutathionylation of STAT3 in the recombinant purified form. This effect was completely reversed by treatment with the reducing agent dithiothreitol, indicating that S-glutathionylation of STAT3 was related to formation of protein-mixed disulfides. Moreover, addition of the bulky negatively charged GSH moiety impairs JAK2-mediated STAT3 phosphorylation, very likely interfering with tyrosine accessibility and thus affecting protein structure and function. Mass mapping analysis identifies two glutathionylated cysteine residues, Cys328 and Cys542, within the DNA-binding domain and the linker domain, respectively. Site direct mutagenesis and in vitro kinase assay confirm the importance of both cysteine residues in the complex redox regulatory mechanism of STAT3. Cells expressing mutant were resistant in this regard. The data presented herein confirmed the occurrence of a redox-dependent regulation of STAT3, identified the more redox-sensitive cysteines within STAT3 structure, and may have important implications for development of new drugs