5 research outputs found
Amide Proton Transfer-Weighted Imaging Detects Hippocampal Proteostasis Disturbance Induced by Sleep Deprivation at 7.0 T MRI
Sleep deprivation leads to hippocampal injury. Proteostasis
disturbance
is an important mechanism linking sleep deprivation and hippocampal
injury. However, identifying noninvasive imaging biomarkers for hippocampal
proteostasis disturbance remains challenging. Amide proton transfer-weighted
(APTw) imaging is a chemical exchange saturation transfer technique
based on the amide protons in proteins and peptides. We aimed to explore
the ability of APTw imaging in detecting sleep deprivation-induced
hippocampal proteostasis disturbance and its biological significance,
as well as its biological basis. In vitro, the feasibility of APTw
imaging in detecting changes of the protein state was evaluated, demonstrating
that APTw imaging can detect alterations in the protein concentration,
conformation, and aggregation state. In vivo, the hippocampal APTw
signal declined with increased sleep deprivation time and was significantly
lower in sleep-deprived rats than that in normal rats. This signal
was positively correlated with the number of surviving neurons counted
in Nissl staining and negatively correlated with the expression of
glucose-regulated protein 78 evaluated in immunohistochemistry. Differentially
expressed proteins in proteostasis network pathways were identified
in the hippocampi of normal rats and sleep-deprived rats via mass
spectrometry proteomics analysis, providing the biological basis for
the change of the hippocampal APTw signal in sleep-deprived rats.
These findings demonstrate that APTw imaging can detect hippocampal
proteostasis disturbance induced by sleep deprivation and reflect
the extent of neuronal injury and endoplasmic reticulum stress