A three-dimensional turbulent compressible flow model for ejector and fluted mixers

A three dimensional finite element computer code was developed to analyze ejector and axisymmetric fluted mixer systems whose flow fields are not significantly influenced by streamwise diffusion effects. A two equation turbulence model was used to make comparisons between theory and data for various flow fields which are components of the ejector system, i.e., (1) turbulent boundary layer in a duct; (2) rectangular nozzle (free jet); (3) axisymmetric nozzle (free jet); (4) hypermixing nozzle (free jet); and (5) plane wall jet. Likewise, comparisons of the code with analytical results and/or other numerical solutions were made for components of the axisymmetric fluted mixer system. These included: (1) developing pipe flow; (2) developing flow in an annular pipe; (3) developing flow in an axisymmetric pipe with conical center body and no fluting and (4) developing fluted pipe flow. Finally, two demonstration cases are presented which show the code's ability to analyze both the ejector and axisymmetric fluted mixers

Modeling of three-dimensional mixing and reacting ducted flows

A computer code, based upon a finite element solution algorithm, was developed to solve the governing equations for three-dimensional, reacting boundary region, and constant area ducted flow fields. Effective diffusion coefficients are employed to allow analyses of turbulent, transitional or laminar flows. The code was used to investigate mixing and reacting hydrogen jets injected from multiple orifices, transverse and parallel to a supersonic air stream. Computational results provide a three-dimensional description of velocity, temperature, and species-concentration fields downstream of injection. Experimental data for eight cases covering different injection conditions and geometries were modeled using mixing length theory (MLT). These results were used as a baseline for examining the relative merits of other mixing models. Calculations were made using a two-equation turbulence model (k+d) and comparisons were made between experiment and mixing length theory predictions. The k+d model shows only a slight improvement in predictive capability over MLT. Results of an examination of the effect of tensorial transport coefficients on mass and momentum field distribution are also presented. Solutions demonstrating the ability of the code to model ducted flows and parallel strut injection are presented and discussed

Toward detailed prominence seismology - I. Computing accurate 2.5D magnetohydrodynamic equilibria

Context. Prominence seismology exploits our knowledge of the linear eigenoscillations for representative magnetohydro- dynamic models of filaments. To date, highly idealized models for prominences have been used, especially with respect to the overall magnetic configurations. Aims. We initiate a more systematic survey of filament wave modes, where we consider full multi-dimensional models with twisted magnetic fields representative of the surrounding magnetic flux rope. This requires the ability to compute accurate 2.5 dimensional magnetohydrodynamic equilibria that balance Lorentz forces, gravity, and pressure gradients, while containing density enhancements (static or in motion). Methods. The governing extended Grad-Shafranov equation is discussed, along with an analytic prediction for circular flux ropes for the Shafranov shift of the central magnetic axis due to gravity. Numerical equilibria are computed with a finite element-based code, demonstrating fourth order accuracy on an explicitly known, non-trivial test case. Results. The code is then used to construct more realistic prominence equilibria, for all three possible choices of a free flux-function. We quantify the influence of gravity, and generate cool condensations in hot cavities, as well as multi- layered prominences. Conclusions. The internal flux rope equilibria computed here have the prerequisite numerical accuracy to allow a yet more advanced analysis of the complete spectrum of linear magnetohydrodynamic perturbations, as will be demonstrated in the companion paper.Comment: Accepted by Astronomy & Astrophysics, 15 pages, 15 figure

Magnetohydrodynamic equilibria of a cylindrical plasma with poloidal mass flow and arbitrary cross section shape

The equilibrium of a cylindrical plasma with purely poloidal mass flow and cross section of arbitrary shape is investigated within the framework of the ideal MHD theory. For the system under consideration it is shown that only incompressible flows are possible and, conscequently, the general two dimensional flow equilibrium equations reduce to a single second-order quasilinear partial differential equation for the poloidal magnetic flux function $\psi$, in which four profile functionals of $\psi$ appear. Apart from a singularity occuring when the modulus of Mach number associated with the Alfv\'en velocity for the poloidal magnetic field is unity, this equation is always elliptic and permits the construction of several classes of analytic solutions. Specific exact equlibria for a plasma confined within a perfectly conducting circular cylindrical boundary and having i) a flat current density and ii) a peaked current density are obtained and studied.Comment: Accepted to Plasma Physics & Controlled Fusion, 14 pages, revte

Viability and Burden of Leishmania in Extralesional Sites during Human Dermal Leishmaniasis

Understanding of the dynamics and distribution of Leishmania in the human host is fundamental to the targeting of control measures and their evaluation. Amplification of parasite gene sequences in clinical samples from cutaneous leishmaniasis patients has provided evidence of Leishmania in blood, other tissues and sites distinct from the lesion and of persistence of infection after clinical resolution of disease. However, there is uncertainty about the interpretation of the presence of Leishmania DNA as indicative of viable parasites. Because RNA is short-lived and labile, its presence provides an indicator of viability. We amplified Leishmania 7SLRNA, a molecule involved in intracellular protein translocation, to establish viability and estimate parasite load in blood monocytes, tonsil swab samples, and tissue fluid from healthy skin of patients with dermal leishmaniasis. Results showed that during active dermal leishmaniasis, viable Leishmania are present in blood monocytes, tonsils and normal skin in quantities similar to that in lesions, demonstrating widespread dissemination of infection and subclinical involvement of tissues beyond the lesion site. Leishmania 7SLRNA will be useful in deciphering the role of human infection in transmission

Increased Virulence of an Epidemic Strain of Mycobacterium massiliense in Mice

Chronic pulmonary disease and skin/soft tissue infections due to non-tuberculous mycobacteria (NTM) of the Mycobacterium chelonae-abscessus-massiliense group is an emerging health problem worldwide. Moreover, the cure rate for the infections this group causes is low despite aggressive treatment. Post-surgical outbreaks that reached epidemic proportions in Brazil recently were caused by M. massiliense isolates resistant to high-level disinfection with glutaraldehyde (GTA). Understanding the differences in the virulence and host immune responses induced by NTM differing in their sensitivity to disinfectants, and therefore their relative threat of causing outbreaks in hospitals, is an important issue.We compared the replication and survival inside macrophages of a GTA-susceptible reference Mycobacterium massiliense clinical isolate CIP 108297 and an epidemic strain from Brazil, CRM-0019, and characterized the immune responses of IFNγ knockout mice exposed to a high dose aerosol with these two isolates. CRM-0019 replicated more efficiently than CIP 108297 inside mouse bone marrow macrophages. Moreover, the animals infected with CRM-0019 showed a progressive lung infection characterized by a delayed influx of CD4+ and CD8+ T cells, culminating in extensive lung consolidation and demonstrated increased numbers of pulmonary CD4+ Foxp3+ regulatory T cells compared to those infected with the reference strain. Immunosuppressive activity of regulatory T cells may contribute to the progression and worsening of NTM disease by preventing the induction of specific protective immune responses.These results provide the first direct evidence of the increased virulence in macrophages and mice and pathogenicity in vivo of the Brazilian epidemic isolate and the first observation that NTM infections can be associated with variable levels of regulatory T cells which may impact on their virulence and ability to persist in the host