Mitochondrial energy metabolism in \kur{Trypanosoma brucei}

The thesis summarizes data gathered on various components of respiratory chain of Trypanosoma brucei. Namely, NADH:ubiquinone oxidoreductase (complex I), alternative NADH:ubiquinone oxidoreductase (NDH2) and mitochondrial glycerol-3-phosphate dehydrogenase are discussed themselves and in broader context of energy metabolism. Also, a work done using RNA interference library is described

Modes of resolution of a debtor's insolvency

The methods of solving the debtor' s insolvency using the "sanitation" principle It has been 5 years since the Czech Insolvency Act was enacted. Therefore is it a good opportunity to summarize the pros and cons of the current situation in this branch of law. The Insolvency Act introduced the concept of two approaches to dealing with the debtor' s insolvency which are new in the Czech legal system: the restructuring and the discharge. Both of them share the "sanitation" principle which enables the debtor to continue their existence after having adopted certain measures. The thesis consists of five major chapters. First section provides the basic explanation of the concept of insolvency and the reasons for the special treatment for the bankruptcy of those who have more creditors. There is also a brief description of the insolvency' s procedure. The second chapter deals with the restructuring, which is the method of solving the insolvency of big companies (entrepreneurs). The third chapter focuses on discharge which is intended for those who are not engaged in business activities (non-entrepreneurs) and which enables them to get rid of their debts. The discharge can be performed in two modes: either by the liquidation of the assets or through the performance of the payment calendar. The chapter four and..

A paradigm shift: The mitoproteomes of procyclic and bloodstream <i>Trypanosoma brucei</i> are comparably complex

A paradigm shift: The mitoproteomes of procyclic and bloodstream <i>Trypanosoma brucei</i> are comparably comple

Pie charts showing distribution of mass spectrometry–identified mitochondrial proteins in PCF (left) and BSF (right) trypanosomes in terms of molecular functions.

<p>A total number of 1,195 and 956 proteins were assigned to PCF and BSF mitoproteome, respectively. Different colors show different metabolic pathways and categories. See also <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006679#ppat.1006679.s001" target="_blank">S1 Table</a>. IFA-mito in PCF (left) and in BSF cell (right). BSF, bloodstream form; Hsp70, heat shock protein 70; IFA-mito, immunofluorescence analysis of a mitochondrial Hsp70; mitoproteome, mitochondrial proteome; PCF, procyclic form.</p

Schematic representation of carbon source metabolism in the bloodstream form of <i>T</i>. <i>brucei</i>.

<p>Red arrows represent enzymatic steps that were experimentally shown to be active in BSF. Green arrows represent enzymatic steps that might be active in BSF because the enzymes (in green) were identified in BSF proteomic data. Glucose-derived metabolites (acetate, pyruvate, succinate, alanine, aspartate) are on a blue background. NADH molecules are on a pink background. Dashed arrows indicate enzymatic steps for which no experimental proof exists. The glycosomal and mitochondrial compartments are indicated. 2-OGDH, 2-oxoglutarate dehydrogenase; AAC, ADP/ATP carrier; AAT, amino acid transporter; ACH, acetyl-CoA thioesterase; AKCT, 2-amino-3-ketobutyrate coenzyme A ligase; Ala TR, alanine transaminase; AOX, alternative oxidase; ASCT, acetate:succinate CoA-transferase; Asp TR, aspartate transaminase; BSF, bloodstream form; cI, complex I (NADH:ubiquinone oxidoreductase); cII, complex II (succinate dehydrogenase); cIII, complex III (cytochrome bc1 complex); cIV, complex IV (cytochrome c oxidase); cV, complex V (F_oF₁ ATPase); cyt, cytosolic; DHAP, dihydroxyacetone phosphate; FH, fumarate hydratase (i.e., fumarase); FR, fumarate reductase; G3P, glyceraldehyde 3-phosphate; GluDH, glutamate dehydrogenase; gly, glycosomal; Gly3P, glycerol 3-phosphate; Gly-3-PDH, glycerol-3-phosphate dehydrogenase; m, mitochondrial; MDH, malate dehydrogenase; PDH, pyruvate dehydrogenase; PEP, phosphoenolpyruvate; PEPCK, phosphoenolpyruvate carboxykinase; PiC, phosphate carrier; ProDH, proline dehydrogenase.</p