Evaluation of the frequency of the IL-28 polymorphism (rs8099917) in patients with chronic hepatitis C using zip nucleic acid probes, Kerman, Southeast of Iran

Polymorphisms in the region of the interleukin IL-28 gene on chromosome 19 have been related with clearance of hepatitis C virus (HCV), a major human pathogen responsible for chronic hepatitis, cirrhosis and hepatocellular carcinoma. About 3 of the world's population is infected with HCV. The long-term response to therapy is influenced by many host and viral factors, and recent evidence has indicated that some host genetic polymorphisms related to IL-28 are the most powerful predictors of virological response in patients with HCV. This study assessed frequency of the IL-28 polymorphism (rs8099917) in 50 patients (39 men and 11 women ) with chronic hepatitis C using ZNA probe real time PCR new method . All patients were tested for genotype of HCV and the HCV viral load. In parallel, the levels of SGOT, SGPT and ALK enzymes were assessed. Treatment using Peg-interferon alpha with ribavirin was conducted for patients and subsequently samples were collected to detect any change in viral load or liver enzyme rates. The overall frequency of the TT allele is 74, TG allele 20 and GG allele 6 and the percent of patients who had T allele was 84. Clear reduction in viral load and liver enzymes was reported in patients with the T allele. Especially for genotype 1 which is relatively resistant to treatment, these alleles may have a role in this decline. In conclusion, we showed that IL-28 polymorphism rs8099917 strongly predicts virological response in HCV infection and that real-time PCR with Zip nucleic acid probes is a sensitive, specific and rapid detection method for detection of SNPs which will be essential for monitoring patients undergoing antiviral therapy

The incidence of prostate cancer in Iran: Results of a population-based cancer registry

Background: Little is known about the epidemiology of prostate cancer in Iranian men. We carried out an active prostate cancer surveillance program in five provinces of Iran. Methods: Data used in this study were obtained from population-based cancer registries between 1996 and 2000. Results: The age-standardized incidence rate of prostate carcinoma in the five provinces was 5.1 per 100,000 person-years. No significant difference was seen in the age-standardized incidence rate of prostate cancer within the provinces studied. The mean±SD age of patients with prostate cancer was 67±13.5 years. Conclusion: The incidence of prostate cancer in Iran is very low as compared to the Western countries. This can partly be explained by lack of nationwide screening program, younger age structure and quality of cancer registration system in Iran

Distribution and molecular identification of some causative agents of streptococcosis isolated from farmed rainbow trout (Oncorhynchus mykiss, Walbaum) in Iran

Over the past few years, the syndrome of streptococcosis has been associated with outbreaks in rainbow trout (Oncorhynchus mykiss, Walbaum) and caused significant economic losses in the aquaculture industry in Iran. The main purpose of this work was molecular identification of some causative agents of streptococcosis in rainbow trout. A total of 520 samples were collected from the head kidney of diseased fish (weight, 50_200g) in 72 farms of 8 provinces in Iran, during 2008 to 2009. Bacterial isolates representing morphology and biochemical profiles of Streptococcus spp. were further confirmed by polymerase chain reaction (PCR). DNA extraction was carried out from a single colony by using the extraction promega kit following the conditions described by the supplier. The PCR assay was developed based on the 16S rRNA and glucose kinase genes of Streptococcus spp. for the rapid and specific detection and identification of this pathogen from different sources. Approximately 40% of specimens were infected to Streptococcus spp. Consequently, five pathogenic species have been identified, including S. iniae in Fars province, S. faecium in Mazandaran province, S. agalactiae in Gilan and Mazandaran provinces, S. dysgalatiae in Lorestan, Kohgiluyeh and Boyerahmad, Gilan and Kermanshah provinces and S. uberis, which was common in all provinces (except Mazandaran and Lorestan). The dominant species (based on important species index) were S. uberis, S. dysgalactiae and S. agalactiae, respectively

Molecular identification of some causative agents of streptococcosis isolated in farmed rainbow trout (Oncorhynchus mykiss, walbaum) in Iran

Streptococcosis is an acute infectious disease that causes mortality in marine and freshwater aquacultures. One of the most important hosts is susceptible to the disease of rainbow trout and during the past decade its industrial production has been growing in Iran. According to FAO, Iran is among the 10 countries producing fish in the world. The most important bacteria causing Strptococcosis includes Strptococcus, Vagococcus and Enterococcus genera. But in all cases, the clinical sings are the same. More than a decade has passed since the first report of this disease in Iran and unfortunately, this disease has become the most important problems of trout production in Iran. Therefor, 72 farms were selected in 8 provinces including Mazandaran, Gilan, Lorestan, CharmahalBakhtiyari, Fars, Kermanshah, Tehran and Kokiloye-Boyer Ahmad and a total 520 moribund and apparently healthy rainbow trout (weight 50 – 200gr) were collected during summer 2008 to 2009. Fish kidney, spleen and liver samples were culture aseptically and finally 206 isolates were identified as gram positive cocci. Using conventional biochemical test, S.uberis, S.agalactiae, S.dysgalactiae, S.faecium and S.inia. Additionally, 172 from 206 isolates were confirmed as S.uberis، S.agalactiae، S.dysgalactiae، S.faecium and S.inia using a PCR assay ased on the results, the most prevalent is belong to S.uberis (isolated from 5 of 7 province), S.dysgalactiae (isolated from 4 of 7 province), S.agalactiae (isolated from 2 of 7 province) and S.faecium and S.inia (from 1 of 7 province) respectively. It is necessary to mention, S.inia was identified just to Fars and any gram positive cocci was isolated from Tehran

Production of bacterial probiotic from trout (Oncorhynchus mykiss) for improvement of immune system and challenge to streptococcosis

This study was conducted for the first time and the effects of probiotic bacteria isolated from the gut of Rainbow trout (Oncorhynchus mykiss). In the first step, after the purification of the isolated bacteria, probiotic properties in vitro was assessed with injection to healthy fish and challenge with three species of pathogenic bacteria in culture media. The second phase of the bacteria isolated in 5 treatments (log 7, 8 and 9 of the lactic bacteria (LAB), Vibrio sp and Pseudomonas sp) and a control treatment on growth and survival, hematological parameters (RBC and WBC cells), immunology and physiology parameters tested during the 60 days of the above parameters after 30 and 60 days and finally at the end, the final assessment was Streptococcosis resistance in fish. The results showed that the isolated bacteria were able to enhance the growth parameters (weight, feed conversion, feed fat, protein efficiency ratio and specific growth rate) and survival. The results showed that log 8 LAB was significant difference with other treatments and control. When using log 8 LAB and Vibrio, the MCV, MCH and MCHC were decreased with no significant difference. The log 8 has the greatest effect on the amount of liver enzyme (AST), IgM and complement component C3, and had significant difference with other treatments. The challenge examination to Streptococcus iniae showed the highest survival in treatments with log 8 (96.66%), and Vibrio (93.33%) and then subjected to other treatments and control are also the least survival (25.38%). The conclusion of that study is the first probiotic properties of bacteria isolated from trout to changes in quality indicators are in particular the LAB bacteria and the second log 8 of LAB had significant positive of changes development and safety, and the fish are resistanted against Streptococcosis

Designing and establishment of ISO/IEC 17025 in 3aboratories of Caspian Sea ecological research center

The project was carried out between March of 2007 and November of 2009.Five laboratories of research center (Clinical pathology, Plankton, Molecular genetics, Pollutants, Analysis Instrument were selected for accreditation. The main stages for establishment of the system consisted of: 1-Conducting a gap analysis to compare the present state of the laboratories with ISO/IEC 17025 Training 2 General requirements for the competence of testing and calibration laboratories Validation of methods Estimation of uncertainty Internal audits 3- Performing of technical and management requirements 4-Submit of quality manual to Iran Accreditation System (IAS) in order to accredit In January of 2010 the laboratories were accredited by Iran Accreditation System (IAS). The main results were including: 1-Increase the accuracy of measurement 2-Improvement of the Repeatability and Reproducibility of the test methods 3-Traceability and standardization of test methods 4- Calibration of measurement instruments 6- Updating of test methods 7-Standardization of physical condition of the laboratories 8- Getting the certification from Iran Accreditation System (IAS)

Persian herbal medicine in functional dyspepsia: A systematic review

Introduction: According to Rome IV, functional dyspepsia is diagnosed with the pres-ence of dyspepsia in the absence of organic or metabolic causes. FD caused by several factors, such as impaired gastric accommodation and hypersensitivity to gastric distention. Several studies have reported the effectiveness of herbal medicine on FD. This article, thus, reviews Persian herbal medicine in FD. Methods: Electronic databases, including Pubmed, Scopus, Cochrane, Embase, Web of science and Ovid, were searched so as to find clinical articles related to dyspepsia and herbal medicine by July 2019. Our search strategies were traditional medicine, complementary and alternative medi-cine, herb, plant, and dyspepsia. We excluded all articles except Persian clinical trials. Results: We found 34 clinical trials with 15 herbs and 4 compound herbal remedies like Asparagus racemosus, Brassica oleracea, Cynara scolymus, Ocimum basilicum, Mentha longifolia, Men-tha pulegium, Mentha piperata, Pimpinella anisum, Nigella sativa, Mastic gum, Curcuma longa, Pistatio Atlantica, Glycyrrhiza glabra, Solanum tuberosum and Zingiber officinale and compound remedies of Rosa damascene & Crocus sativus, Trachyspermum copticom & Apium graveolence, Carum carvi & Mentha pipperata, Gingiber officinalis & Cynara scolymus are effective in functional dyspepsia. Conclusion: Many people use herbal and traditional remedies for the treatment of disorders such as gastrointestinal disorders, especially in Asian countries. Several studies reported the efficacy of herbal medicine in functional dyspepsia. Although their mechanisms are not fully understood, it seems they can modulate GI motility and improve symptoms of FD. © 2021 Bentham Science Publishers

Demographic and Clinical Features of Ulcerative Colitis Patients in Kerman City during 2005-2007

Abstract: Background & Aims: Ulcerative colitis is one of the inflammatory bowel diseases with unknown etiology. Genetic and environmental factors are thought to be effective in this disease. According to the limited studies in our country, general assessment of demographic features and clinical symptoms seems to be essential. Methods: In this prospective and cross-sectional study, 85 cases of ulcerative colitis referred to the 3 gastroenterologist offices in Kerman city were evaluated during 2005-2007. The diagnosis was confirmed based on clinical features, colonoscopy, pathology and ruling out of other causes. Data were collected through direct interview and analyzed by SPSS 15 software. Results: Mean age at the time of diagnosis was 33.31_13.12. Duration of symptoms onset until diagnosis was 8 months. Male to female ratio was 0.8/1 and the most important clinical manifestations were rectorhagia and bloody diarrhea (92.9%), abdominal pain (20%), mucoid diarrhea (13.1%), weight loss (11.2%) and fever (4.4%). The pattern of disease was chronic intermittent in 83.5%, chronic continuous in 13% and acute self-limited in 3.5%. Anatomically disease was limited to the rectum (8.3%), recto sigmoid (23.5%), recto sigmoid and descending colon (28.5%), transverse colon (21.2%) and pan colitis. (18.8%). Extra intestinal manifestations were seen in 22.35% of cases of whom 12.96% had oral involvement, 5.85% had hepatic involvement and 3.53% had musculoskeletal involvement. Conclusion: Recurrent inflammatory bowel disease is one of the important reasons of referral to gastroenterologists. Ulcerative coilitis in Kerman is similar to other countries of world epidemiologically, demographically and clinically but its seems that severe clinical courses, extra intestinal manifestations and second old age peak to be less comparing to other studies. Keywords: Ulcerative colitis, Signs and symptom

A Case Report of Gastrointestinal Bleeding Due to Gastric Lipoma

Abstract: Benign Gastric tumors are rare and generally account for less than 10% of all stomach tumors. Gastric lipoma is a rare tumor that constitutes approximately 3% of all benign tumors of the stomach and mainly is seen as a submucosal mass. Most gastric lipoma are asymptomatic and are found accidentally. Occasionally they can cause symptoms such as gastrointestinal bleeding, obstruction, abdominal pain and intussuception. CT scan and endoscopy are helpful in diagnosis. The main modality of treatment is surgery. Diagnosis is confirmed by histology. In this article, a patient with weakness, fatigue, and melena complaints is presented. On gastric endoscopy a yellowish submucosal mass was seen in antrum. Abdominal CT scan disclosed a mass with fat density resembling lipoma. The patient underwent surgery and diagnosis of lipoma was confirmed by histology. Although gastric lipoma is rare, it should be considered in the assessment and differential diagnosis of hemorrhagic submucosal masses in the stomach. Keywords: Lipoma, Gastrointestinal bleedin