The project was carried out between March of 2007 and November of 2009.Five laboratories of research center (Clinical pathology, Plankton, Molecular genetics, Pollutants, Analysis Instrument were selected for accreditation. The main stages for establishment of the system consisted of: 1-Conducting a gap analysis to compare the present state of the laboratories with ISO/IEC 17025 Training 2 General requirements for the competence of testing and calibration laboratories Validation of methods Estimation of uncertainty Internal audits 3- Performing of technical and management requirements 4-Submit of quality manual to Iran Accreditation System (IAS) in order to accredit In January of 2010 the laboratories were accredited by Iran Accreditation System (IAS). The main results were including: 1-Increase the accuracy of measurement 2-Improvement of the Repeatability and Reproducibility of the test methods 3-Traceability and standardization of test methods 4- Calibration of measurement instruments 6- Updating of test methods 7-Standardization of physical condition of the laboratories 8- Getting the certification from Iran Accreditation System (IAS)

Behroozi, Sh.

Binaei, M.

Doostar, M.

Eslami, F.

Esmaeili, Fariba

Farabi, V.

Firoozkandian, S.H.

Ganjiyan, A.

Ghara, K.

Ghiaci, M.

Gholamipoor, S.

Golaghaei, M.

Habibi, F.

Kayhansani, A.R.

Khodaparast, N.

Lalooei, F.

Makhloogh, A.

Najafpoor, Sh.

Nasrollahzadeh Saravi, H.

Nasrollhtabar, A.

Nayerani, M.

Noroozian, M.

Poorgholam, R.

Roshantabari, M.

Rostamian, M.

Saeidii, A.A.

Salmani, A.

Taghavi, M.J.

Vahedi, F.

Varedi, A.

Yoonesipoor, H.

Zahedi, A.

Aquatic Commons

   
                                                
           
                       
            
   
                        
Gap Analysis
   
MINISTRY OF JIHAD - E - AGRICULTURE 
AGRICULTURAL RESEARCH, EDUCATION & EXTENTION ORGANIZATION 
IRANIAN FISHERIES RESEARCH ORGANIZATION- Caspian Sea Ecology Research 
Center       
Title:   
Designing and Establishment  
of ISO/IEC 17025 in Laboratories 
 of Caspian Sea Ecological  Research Center      
Executor :  
Fariba Esmaeili      
                                                                                                                                                                                                                           
Registration Number 
2010.1596 
                      
Ministry of Jihad  e  Agriculture  
AGRICULTURAL RESEARCH, EDUCATION & EXTENTION ORGANIZATION 
IRANIAN  FISHERIES  RESEARCH  ORGANIZATION   Caspian Sea Ecology Research Center 
Title : Designing and Establishment of ISO/IEC 17025 in Laboratories of Caspian Sea 
Ecological  Research Center 
Apprpved Number: 2-76-12-8603-86055   
Author:  Fariba Esmaeili 
Executor : Fariba Esmaeili   
Collaborator:Salmani,F.Lalooei,R.Poorgholam,S.Gholamipoor,M.Noroozian,A.Varedi,M.Rosha
ntabari,M.Binaei,M.J.Taghavi,H.Nasrollahzadehsaravi,A.Nasrollhtabar,N.Khodaparast,M.Golagh
aei,M.T.Rostamian,A.R.Kayhansani,A.Zahedi,F.Habibi,SH.Firoozkandian,F.Vahedi,H.Yoonesip
oor,A.Makhloogh,M.Doostar,F.Eslami,SH.Behroozi,A.A.Saeidi,A.Ganjiyan,M.Nayerani,M.Ghia
ci,V.Farabi,SH.Najafpoor,K.Ghara. 
Advisor(s):A.A.Motallebi,N.Poorang,M.R.Tooraji  
Location of execution :  Mazandaran province 
Date of Beginning : 2007 
Period of execution : 2Years & 6Months 
Publisher : Iranian Fisheries Research Organization 
Circulation : 15 
Date of publishing : 2010 
All Right Reserved . No Part of this Publication May be Reproduced or Transmitted 
without indicating the Original Reference 
ISO/IEC
 
ISO/IEC(Accreditation)(Calibration)
 ISO/IECISO/IEC Guide
EN
A2LA
GA-A, DA tech, DAch, DAP
UKAS M10
  UKAS 2000; Stephanie, B. 1999) (UKAS 1997;
(ABS)
ASQ-ANSIACLASSLA2A1999, ANSI 
(A2LA)
ISO/IEC 17025:2005
A2LA
IAS
NVLAPASCLD
NATATELARC
TELARC/NATAUKASFINAS
DANAK
PALCAN
(NABL)
ILAC
(ISO/IEC Guide58-which became 
ISO/IEC 17011)
ILAC)2002 17G-ILAC( 
EAAPLACSADCAIAAC
ILAC
 (ISO 1993 ;  Cigler, J. L. and R. W. Vanda1994 ; ANSI 1999 ; UKAS 1997 ; Stephanie, B. 1999;   UKAS 
2000   ;  ILAC-G17 2002)
 
  (ISO 1993 ;  Cigler, J. L. and R. W. Vanda1994 ;  UKAS 1997  ; ; Stephanie, B. 1999;    ANSI 1999 ; 
UKAS 2000   ;  ILAC-G17 2002) 

 (Gap Analysis)
 (Uncertainty) 
(Validation) 
 ISO 1990 ISO 2006
 Sampling plan
 ISO 1990 ISO 2006
IAS

 (Audit trial)
L-F-401-01
 
L-F-401-02
l-P-413-01
L-J-502-01
L-L-401-01
 ISO/IEC 17025:2005
L-L-403-01
L-P-403-
01
L-P-403-01
L-L-403-01
L-P-403-01
L-P-403-01
L-P-403-01
L-P-404-01
L-P-404-01
L-P-405-01
L-P-406-01
L-P-506-01
L-P-406-01
L-P-506-01
 L-P-505-01
L-P-404-01
L-P-404-01
L-P-411-01
L-P-412-01
L-P-409-01
L-P-411-01
  
 L-P-
411-01
 L-P-411-01
 L-P-412-01
L-P-413-01
ISO/IEC 17025L-P-414-01
L-P-
411-01
L-P-413-01
L-P-414-01
L-P-415-01
 
 L-P-504-01
L-J-502
L-P-502-01
L-P-502-
01
L-W-503-01
L-P-409-01
 L-F-503-01
L-P-403-01
L-P-404-01
ISO/IEC/17025
L-P-404-01
(Modified)(Amplified)
L-P-504-01
1993ISO 
L-P-504-01
L-P-413-01
 L-P-504-02
L-P-413-01
L-L-505-01
L-P-505-01
L-F-505-01
L-P-
505-01
L-P-505-01
L-P-409-01
L-P-505-01
L-P-505-01
L-P-505-01
(SI)
 L-P-505-01
L-P-506-01
L-L-506-01
L-P-409-01
L-P-506-01
L-P-506-01
L-P-507-01
L-P-404-01
L-P-508-01
L-P-407-01
L-P-508-01
L-L-508-01
 L-P-509-01
L-L-509-01
L-P-
412-01
L-P-510-01
ISO/IEC 17025: 2005
ISO/IEC 17025
 
 L-P-415-01-00
  
  Office
  AAS,HPLC,GC 
AAS,HPLC,GC
 
Office
 ISO /IEC 17025 
 2003     Office
ISO /IEC 17025 
 office 
 PCR 
  
Office
 2003     Office
 ISO/IEC/ 17025
L-QM-00
(L-P-502-01)
(L-P-404-01)
(L-W-404-01)
(L-P-415-01)
(L-P-409-01)
(L-P-403-01)
(L-P-411-01)
(L-P-412-01)
(L-P-405-01)
(L-P-510-01)
(L-P-508-01)
(L-P-406-01)
(L-P-414-01)
(L-P-507-01)
(L-P-408-01)
(L-P-505-01)
(L-P-506-01)
(L-P-504-01)
(L-P-504-01)
(L-P-509-01)
(L-Q-P)
(L-J-502)
(L-F-401-01)
(L-F-503-01)
(L-W-503-01)
(L-W-505-11)
(L-W-505-01)
(L-F-403-01)
(L-F-403-02) 
(L-F-404-03)
(L-F-406-01)
(L-F-406-02)
(L-F-406-03)
(L-F-408-01)
(L-F-409-01)
(L-F-411-01)
(L-F-414-01)
(L-F-414-04
(L-F-414-03)
NCRNCR (L-F-414-04)
(L-F-415-01)
(L-F-502-01)
(L-F-502-02)
(L-F-502-03)
 (L-F-503-01)
(L-F-504-01)
(L-F-505-01)
(L-F-505-02)
(L-F-406-04)
(L-F-406-05)
(L-F-406-04)
(L-F-403-01)
(L-F-403-02)
(L-F-405-01)
(L-F-406-01)
(L-F-406-02)
(L-F-413-01)
(L-L-505-01)
(L-L-506-01)
MSDS(L-F-406-03)
PCR(Corbet research)(L-W-505-01) 
L-W-505-02)- (Auto-Q)
L-W-505-03
L-W-505-04
L-W-505-05
L-W-505-06
L-W-505-08
L-W-505-07
L-W-505-09
L-W-505-10
L-W-505-12
L-W-505-13
L-W-505-15
L-W-505-16
L-W-505-17
L-W-505-42
L-W-505-43
L-W-505-07
L-W-505-10
L-W-505-12
L-W-505-13
L-W-505-15
L-W-505-17
(L-W-505-23
L-W-505-24
 L-W-505-25
L-W-505-27
L-W-505-30
L-W-505-31
L-W-505-33
L-W-505-34
(L-W-505-35
(L-W-505-36
(L-W-505-37)
(L-W-505-38
(L-W-505-39
(L-W-505-42
L-W-505-44
 (Cold plate)( L-W-505-45
UV(L-W-505-46)
(L-W-504-02-00
L-W-504-03-00
L-W-504-04-00
(L-W-504-05-00
L-W-504-06-00
L-W-504-07-00
HPLC(L-W-505-18)
(L-W-505-49
(L-W-505-20
pH(L-W-505-21)
(L-W-505-22)
(L-W-505-30
(L-W-505-23
 (L-W-505-24
(L-W-505-25
(L-W-505-26
(L-W-505-27
(L-W-505-32
(L-W-505-41)
L-W-505-07
(L-W-505-17
pH(L-W-505-21
(L-W-505-28)
(L-W-505-29)(
 (L-W-505-31
1- Eaton,A.D. Clesceri,L.S.,Rice,E.W. abd Greenberg,A.E., 2005. 
Standard Method.America Public Health Association,Washington,Dc 
2001-3710 
2-Wetzel, R. G. and Likens, G. E. 2000. Limnological Analyses. Springer-
Verlag. P: 424.  
3. Carmelo R. Tomas 1997. Identifying marine phytoplankton. Publication 
Harcourt Brace company. P: 558. 
4- Academy of science in USSR, The algae of Caspian sea. 1968. 
Publication Leningrad company.  P:290  
5-proshkina-Lavrenko.  A. E. 1951.The Identifying of freshwater 
phytoplankton. Moscow. P: 620. 
6-Kasimov, A. 2004. Ecology of the Caspian Sea plankton. Adiloglu, Baku. 
P: 540.  
7-Hartley, B., H.G.Barber, J.R.Carter, and P.Sims .1996.An Atlas of 
British Diatoms. Biopress Limited, Bristol, UK. P: 600. 
8- John D. Wehr, Robert G. Sheath.2003.Freshwater Algae of North America: 
Ecology and Classification. Academic Press.USA. P:918  
1- Eaton,A.D. Clesceri,L.S.,Rice,E.W. abd Greenberg,A.E., 2005. 
Standard Method.America Public Health Association,Washington,Dc 
2001-3710 
2- Birshtina,A.,Vinogradova,L.,Kondakova,n.n.,MKyn,M.C.,Actakhopy ,
T.V.,romanova,n.n.1968.Atlas of Ivertebrate of Caspian sea.mosko.(In 
Russian). P: 415 
3- Kutikova, l.A. 1970. Rotatoria. Leningrad Science.(In Russian). P: 744. 
4- Manolova, E. F. 1964. Cladocera. Mosco.(In Russian).  P:326. 
5- Wetzel, R. G. and Likens, G. E. 2000. Limnological Analyses. Springer-
Verlag. P:424.  
6- Harris, R., Wiebe, P., Lenz, J., Skjoldal, H. R. and Huntly, M. 
2000.ICES Zooplankton Methodology Manual. Academic press. P: 686. 
7- Boltovskoy, D. 1999. South Atlantic Zooplankton. Backhuys publisher. 
P:868. 
8- Parry, L. 1992. Protozoan plankton ecology. Chapman&Hal.  P:231. 
9-Tsalolikhin, S. J. 1997. Key to freshwater invertebrates of Russia. P:627.  
 APHA,2005.Standard Methods for Examination of 
water.20th edition,Method ,Washington D.c.,p3-1 to 
3-107. 
MOOPAM 1999 .Manual of Oceanographic 
Observation and Pollutant Analyses 
Methods .Regional Organization for Protection of the 
Marine Environment (ROPME),kuwait.pp:IV-VI 
MOOPAM 1999 .Manual of Oceanographic 
Observation and Pollutant Analyses 
Methods .Regional Organization for Protection of the 
Marine Environment (ROPME),kuwait.pp: VI-1 to 
49 
EPA:1993,pesticides module method# :508 
G2, G1, B2, B1 
*Fe,Pb,Cu,Cd,CO,Cr,Zn,Ni,Hg 
** PAHs 
***Delta- BHC, Heptacholor, Heptacholor Epoxide, Alfa  BHC, Beta & Gama  BHC, Aldrine, Alfa 
Endosulfan, DDE, DDD, Dieldrin, Endrine, Endrine Aldehyde, DDT, Endosulfan Sulfate, Beta-
Endosulfan  
(Recovery)
APHA,2005.Standard Methods for Examination of water.20th 
edition,Method ,Washington D.c.,p 3-1 to 3-24. 
MOOPAM 1999 .Manual of Oceanographic Observation and 
Pollutant Analyses Methods .Regional Organization for 
Protection of the Marine Environment (ROPME),kuwait.pp: 
V-28 to V-30
MOOPAM 1999 .Manual of Oceanographic Observation and 
Pollutant Analyses Methods .Regional Organization for 
Protection of the Marine Environment (ROPME),kuwait.pp:
VI-7 , III 12 to 16 
EPA:1993,pesticides module method# :508- 1 to 508-29 
APHA,2005.Standard Methods for Examination of water.20th 
edition,Method ,Washington D.c.,p5-47 to 5-53. 
*Fe,Pb,Cu,Cd,CO,Cr,Zn,Ni,Hg
** PAHs 
***Delta- BHC, Heptacholor, Heptacholor Epoxide, Alfa  BHC, Beta & Gama  BHC, Aldrine, Alfa 
Endosulfan, DDE, DDD, Dieldrin, Endrine, Endrine Aldehyde, DDT, Endosulfan Sulfate, Beta-
Endosulfan 
 -Ronald j. Roberts, (2001),Fish Pathology, Third  edition  
W.B.Saunders  
- theory and Practice of Histological techniques (2002) john D. 
Bancroft & marilyn Gamble . Churchill Livingston  
-Buller, N.B. (2004) bacteria from fish and other aquatic animals: a practical   
identification manual  
R.B.C
W.B.C
Hb
PCVHct
ESR
CT
MCV
MCH
MCHC
-Basic techniques in Fish Haematology 
(www.aqualex .org/elearing/fish_haematology/english/index.html 
SGot,SGPt,CPK-
LDH,ALP,Amy, 
P3-,Fe2+,Ca2+ 
Igm
C3-C4 
total protein, 
Albumin
  
 
Arlene, J. , Rodney, A. Bray and David, 1. Gibson .(2006) Keys to the Trematoda  volume 
2 
- Frantisek, M. (1998) Nematodes of freshwater fishes of the neotropical region.  
- Khalil,L.F. and Jones, A. and Bray, R. A. (1994) Keys to the Cestode parasites of 
vertebrates. 
- Lom,J. Dykova, I. (1992) Protozoan parasites of fishes.
Hoekstra R.A., Frisvad E.S., Tenborg. O.F. ,( 2000), Introduction to Food and airborne 
fungi, Sixth edition, Centraalbureau voor schimmelcultures , Utrecht 
Larone, D.H., (2002), Medically important fungi, Forth edition, ASM press, USA 
Khulbe,R.D., 2001, The manual of aquatic Fungi- Daya publishing house , India. 
 Ronald .J.Roberts,(2001) ,Fish pathology, Third  edition  ,W.B.Saunders,USA  . 
 DNA Sambrok and Russell, 2006. The condensed 
protocols from Molecular Cloning :A 
laboratory Manual: Cold Spring Harbor 
Laboratory Press: 
PCR DNASambrok and Russell, 2006. The condensed 
protocols from Molecular Cloning :A 
laboratory Manual: Cold Spring Harbor 
Laboratory Press: 
PCR
RFLPRAPDSambrok and Russell, 2006. The condensed 
protocols from Molecular Cloning :A 
laboratory Manual: Cold Spring Harbor 
Laboratory Press:Spring Harbor Laboratory 
Press:Laboratory Press:  
RAPD
ISO/IEC 17025
 pH

 IAS
 IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
IAS 
 IAS 
IAS 
IAS 
IAS 

  
 5.  ANSI (1999). American National Standard for Calibration  Calibration Laboratories and Measuring and 
Test Equipment General Requirements. American National Standards Institute (ANSI), ANSI/NCSL Z540-
1, New York.  
6. Cigler, J. L. and R. W. Vanda (1994). National Voluntary Laboratory Accreditation Program 
Procedures and General Requirements. NVLAP Handbook 150, U.S. Department of Commerce, Technology 
Administration, National Institute of Standards and Technology (NIST). Gaithersburg, Maryland.Elison, S. L. 
R., M. Rosslein and A. Williams (2000). Quantifying uncertainty in analytical Measurement. 
EURACHEM/CITAC Guide.  
7. ILAC-G17 (2002). Introducing the Concept of Uncertainty of Measurement in Testing in Association 
with the Application of the Standard ISO/IEC 17025. 
8. ISO (1993). Guide to the Expression of uncertainty in measurement. International Standards 
Organization (ISO), Geneva, Switzerland.  
9. ISO (1990). General Requirements for the Competence of Calibration and Testing Laboratories. 
ISO/IEC Guide 25, Third edition, International Standards Organization (ISO), Geneva, Switzerland. 
10. ISO (2006). General Requirement for the Competence of testing and Calibration Laboratories.  
International Organization for Standardization/ International Electrotechnical Commission ISO/IEC 
17025:2005.  
11. Stephanie, B. (1999). A Beginner's Guide to Uncertainty of Measurement. Measurement Good Practice 
Guide No. 11, National Physical Laboratory, Teddington, UK. 
12. UKAS (1997). The Expression of Uncertainty and Confidence in Measurement. United Kingdom 
Accreditation Service (UKAS), M3003. 
13. UKAS (2000). The Expression of Uncertainty in Testing. United Kingdom Accreditation Service 
(UKAS) , LAB 12.          
 Abstract 
The project was carried out between March of 2007 and November of 2009.Five laboratories of research center 
(Clinical pathology, Plankton , Molecular genetic, Pollutants ,  Analysis  Instrument were selected for 
accreditation. 
The main stages for establishment of the system consisted of:   
1-Conducting a gap analysis to compare the present state of the laboratories with ISO/IEC 17025 
Training 2 
General requirements for the competence of testing and calibration laboratories
 
Validation of methods 
Estimation of uncertainty  
Internal audits 
3- Performing of technical and management requirements  
4-Submit of quality manual to Iran Accreditation System (IAS) in order to accredit 
 In January of 2010 the laboratories were accredited by Iran Accreditation System (IAS). 
The main results were including: 
1-Increase the accuracy of measurement  
2-Improvement of the Repeatability and Reproducibility of the test methods  
3-Traceability and standardization of test methods  
4- Calibration of measurement instruments 
6- Updating of test methods   
7-Standardization of physical condition of the laboratories  
8- Getting the certification from Iran Accreditation System (IAS). 
Key words: 
Standardization, ISO/IEC 17025, laboratory, accreditation,calibration, ecological institute of Caspian sea    
This document was created with Win2PDF available at http://www.daneprairie.com.
The unregistered version of Win2PDF is for evaluation or non-commercial use only.


Designing and establishment of ISO/IEC 17025 in 3aboratories of Caspian Sea ecological research center

http://aquaticcommons.org/25216/1/2010.1596.pdf

Designing and establishment of ISO/IEC 17025 in 3aboratories of Caspian Sea ecological research center

Abstract

Similar works

Full text

Available Versions

Aquatic Commons