Deficiency of COX-1 gene expression in mice reduces the infarction volume in a 24-hour permanent focal cerebral ischemia model

Inflammatory reactions have an important role in the ischemic pathophysiology. Cyclooxygenase (COX) is the key enzyme in converting arachidonic acid to prostanoids that, in turn, are the major contributors to the intrinsic inflammatory response. In this study, we used COX-1-null mice to investigate the role of COX-1 gene expression in a permanent focal cerebral ischemia model. Adult littermates (wild type +/+, heterozygous +/-, and homozygous -/-), were used. Genetic status was determined using a PCR analysis. The mice were anesthetized with chloral hydrate (350 mg/kg, IP). Right femoral artery was cannulated for monitoring of arterial blood pressure and heart rate. Rectal temperature was kept normal throughout anesthesia. Cerebral blood flow was monitored by Laser-Doppler Flowmetry. Permanent focal cerebral ischemia was achieved by passing a monofilament suture via the right external and internal carotid arteries to occlude the right middle cerebral artery (MCA) at its origin. Mice were killed by decapitation at 24 hours after MCA occlusion. The brains were cut into 2-mm coronal slices for staining with 2% tetrazolium chloride to reveal the infarct. The infarction volume was quantified using computer-assisted image analysis. Relative infarction volumes were, in mean ?SEM, 42.67 ?3.72% (n=6) in COX-1 +/+ mice, 25.97 ?8.48% (n=6) in COX-1 +/- mice, and 28.13 ?6.22% (n=6) in COX-1 -/- mice, respectively. There was no significant difference in physiological parameters and cerebral perfusion among the groups. Our results demonstrated that lack of COX-1 gene expression might reduce infarction volume in a 24-hour permanent MCA occlusion model in mice.published_or_final_versio

A 3D Face Modelling Approach for Pose-Invariant Face Recognition in a Human-Robot Environment

Face analysis techniques have become a crucial component of human-machine interaction in the fields of assistive and humanoid robotics. However, the variations in head-pose that arise naturally in these environments are still a great challenge. In this paper, we present a real-time capable 3D face modelling framework for 2D in-the-wild images that is applicable for robotics. The fitting of the 3D Morphable Model is based exclusively on automatically detected landmarks. After fitting, the face can be corrected in pose and transformed back to a frontal 2D representation that is more suitable for face recognition. We conduct face recognition experiments with non-frontal images from the MUCT database and uncontrolled, in the wild images from the PaSC database, the most challenging face recognition database to date, showing an improved performance. Finally, we present our SCITOS G5 robot system, which incorporates our framework as a means of image pre-processing for face analysis

Mutagenesis of Snu114 domain IV identifies a developmental role in meiotic splicing

<p>Snu114, a component of the U5 snRNP, plays a key role in activation of the spliceosome. It controls the action of Brr2, an RNA-stimulated ATPase/RNA helicase that disrupts U4/U6 snRNA base-pairing prior to formation of the spliceosome’s catalytic centre. Snu114 has a highly conserved domain structure that resembles that of the GTPase EF-2/EF-G in the ribosome. It has been suggested that the regulatory function of Snu114 in activation of the spliceosome is mediated by its C-terminal region, however, there has been only limited characterisation of the interactions of the C-terminal domains. We show a direct interaction between protein phosphatase PP1 and Snu114 domain ‘IVa’ and identify sequence ‘YGVQYK’ as a PP1 binding motif. Interestingly, this motif is also required for Cwc21 binding. We provide evidence for mutually exclusive interaction of Cwc21 and PP1 with Snu114 and show that the affinity of Cwc21 and PP1 for Snu114 is influenced by the different nucleotide-bound states of Snu114. Moreover, we identify a novel mutation in domain IVa that, while not affecting vegetative growth of yeast cells, causes a defect in splicing transcripts of the meiotic genes, <i>SPO22, AMA1</i> and <i>MER2</i>, thereby inhibiting an early stage of meiosis.</p

Numerical investigation of viscous effects on the gap resonance between side-by-side barges

This paper presents a numerical study of the gap resonance between two side-by-side barges by using a multiphase Navier-Stokes equations model. In order to verify the multiphase flow model, it is firstly applied to simulate a two-dimensional gap resonance problem for two fixed boxes under various wave conditions. A comparison of the free surface elevations obtained on successively refined grids confirms the mesh convergence of numerical solutions. The calculated wave elevation response amplitude operators (RAOs) in the gap compare well with the experimental measurements. The multiphase flow model is further extended to calculate a three-dimensional gap resonance problem for two adjacent rectangular barges. The computed free surface RAOs in the gap also agree well with the experimental results. A close examination of the flow velocity and vorticity in the gap region at the piston resonant mode reveals that large amount of vortices are generated by the sharp corners of the two barges and shed downwards, which provide an effective mechanism to dissipate the flow kinematic energy and to reduce the wave elevation in the gap. On the contrary, rounded corners are not able to induce the same level amount of vortices to dampen the gap resonance. The effects of incident wave steepness on the viscous damping associated with the twin-barge system are highlighted

On the Relation Between Jupiter's Aurora and the Dawnside Current Sheet

Jupiter's auroral emission is a spectacular phenomenon that provides insight into energy release processes related to the coupling of its magnetosphere and ionosphere. This energy release is influenced by solar wind conditions. Using joint observations from Juno and the Hubble Space Telescope (HST), we statistically investigate the relationship between auroral power and current sheet variations under different solar wind conditions. In this study, we reveal that during global main auroral brightening events that are closely connected to solar wind compressions, the dawn side current sheet is substantially thinner than during times when a quiet auroral morphology is present. Furthermore, the total current intensity in the current sheet is found to increase under solar wind compression conditions compared to the quiet period. These findings provide important observational evidence for how magnetospheric dynamics driven by solar wind behavior affect auroral activity, deepening our understanding of the coupling between Jupiter's magnetosphere and ionosphere

Effects of cyclooxygenase-1 and -2 gene disruption on Helicobacter pylori-induced gastric inflammation

Background. Cyclooxygenases (COXs) play important roles in inflammation and carcinogenesis. The present study aimed to determine the effects of COX-1 and COX-2 gene disruption on Helicobacter pylori-induced gastric inflammation. Methods. Wild-type (WT), COX-1 and COX-2 heterozygous (COX-1 +/- and COX-2 +/-), and homozygous COX-deficient (COX-1 -/- and COX-2 -/-) mice were inoculated with H. pylori strain TN2 and killed after 24 weeks of infection. Uninfected WT and COX-deficient mice were used as controls. Levels of gastric mucosal inflammation, epithelial cell proliferation and apoptosis, and cytokine expression were determined. Results. COX deficiency facilitated H. pylori-induced gastritis. In the presence of H. pylori infection, apoptosis was increased in both WT and COX-deficient mice, whereas cell proliferation was increased in WT and COX-1-deficient, but not in COX-2-deficient, mice. Tumor necrosis factor (TNF)-α and interleukin-10 mRNA expression was elevated in H. pylori-infected mice, but only TNF-α mRNA expression was further increased by COX deficiency. Prostaglandin E 2 levels were increased in infected WT and COX-2-deficient mice but were at very low levels in infected COX-1-deficient mice. Leukotriene (LT) B 4 and LTC 4 levels were increased to a similar extent in infected WT and COX-deficient mice. Conclusions. COX deficiency enhances H. pylori-induced gastritis, probably via TNF-α expression. COX-2, but not COX-1, deficiency suppresses H. pylori-induced cell proliferation. © 2006 by the Infectious Diseases Society of America. All rights reserved.published_or_final_versio

Heterogeneity of Paucigranulocytic Asthma: A Prospective Cohort Study with Hierarchical Cluster Analysis.

BACKGROUND: Asthma, a heterogeneous disease, can be divided into 4 inflammatory phenotypes using induced sputum cell counts-eosinophilic asthma (EA), neutrophilic asthma (NA), mixed granulocytic asthma, and paucigranulocytic asthma (PGA). Although research has focused on EA and NA, there is little known about PGA. OBJECTIVE: To study the heterogeneity of PGA and identify possible PGA clusters to guide clinical treatment. METHODS: Patients with PGA were grouped by hierarchical cluster analysis and enrolled into a prospective cohort study to validate the clusters, relative to future risk of asthma exacerbations in a real-world setting. Clusters were validated by tree analysis in a separate population. Finally, we explored PGA stability. RESULTS: Cluster analysis of 145 patients with PGA identified 3 clusters: cluster 1 (n = 110, 75.9%) was "mild PGA," cluster 2 (n = 20, 13.8%) was "PGA with psychological dysfunction and rhinoconjunctivitis and other allergic diseases," and cluster 3 (n = 15, 10.3%) was "smoking-associated PGA." Cluster 3 had significantly increased risk of severe exacerbation (relative risk [RR] = 6.43, P = .01), emergency visit (RR = 8.61, P = .03), and hospitalization (RR = 12.94, P < .01). Results of the cluster analysis were successfully validated in an independent PGA population classified using decision tree analysis. Although PGA can transform into or develop from other phenotypes, 70% were stable over time. CONCLUSIONS: Among 3 identified PGA clusters, cluster 3 had a higher risk of severe exacerbation. PGA heterogeneity indicates the requirement of novel targeted interventions

Aggregated impact of allowance allocation and power dispatching on emission reduction

Climate change has become one of the most important issues for the sustainable development of social well-being. China has made great efforts in reducing CO2 emissions and promoting clean energy. Pilot Emission Trading Systems (ETSs) have been launched in two provinces and five cities in China, and a national level ETS will be implemented in the third quarter of 2017, with preparations for China’s national ETS now well under way. In the meantime, a new round of China’s electric power system reform has entered the implementation stage. Policy variables from both electricity and emission markets will impose potential risks on the operation of generation companies (GenCos). Under this situation, by selecting key variables in each domain, this paper analyzes the combined effects of different allowance allocation methods and power dispatching models on power system emission. Key parameters are set based on a provincial power system in China, and the case studies are conducted based on dynamic simulation platform for macro-energy systems (DSMES) software developed by the authors. The selected power dispatching models include planned dispatch, energy saving power generation dispatch and economic dispatch. The selected initial allowance allocation methods in the emission market include the grandfathering method based on historical emissions and the benchmarking method based on actual output. Based on the simulation results and discussions, several policy implications are highlighted to help to design an effective emission market in China