Unsupervised detection and localization of structural textures using projection profiles

Cataloged from PDF version of article.The main goal of existing approaches for structural texture analysis has been the identification of repeating texture primitives and their placement patterns in images containing a single type of texture. We describe a novel unsupervised method for simultaneous detection and localization of multiple structural texture areas along with estimates of their orientations and scales in real images. First, multi-scale isotropic filters are used to enhance the potential texton locations. Then, regularity of the textons is quantified in terms of the periodicity of projection profiles of filter responses within sliding windows at multiple orientations. Next, a regularity index is computed for each pixel as the maximum regularity score together with its orientation and scale. Finally, thresholding of this regularity index produces accurate localization of structural textures in images containing different kinds of textures as well as non-textured areas. Experiments using three different data sets show the effectiveness of the proposed method in complex scenes.(C)2010 Elsevier Ltd. All rights reserved

Automatic detection and segmentation of orchards using very high-resolution imagery

Cataloged from PDF version of article.Spectral information alone is often not sufficient to distinguish certain terrain classes such as permanent crops like orchards, vineyards, and olive groves from other types of vegetation. However, instances of these classes possess distinctive spatial structures that can be observable in detail in very high spatial resolution images. This paper proposes a novel unsupervised algorithm for the detection and segmentation of orchards. The detection step uses a texture model that is based on the idea that textures are made up of primitives (trees) appearing in a near-regular repetitive arrangement (planting patterns). The algorithm starts with the enhancement of potential tree locations by using multi-granularity isotropic filters. Then, the regularity of the planting patterns is quantified using projection profiles of the filter responses at multiple orientations. The result is a regularity score at each pixel for each granularity and orientation. Finally, the segmentation step iteratively merges neighboring pixels and regions belonging to similar planting patterns according to the similarities of their regularity scores and obtains the boundaries of individual orchards along with estimates of their granularities and orientations. Extensive experiments using Ikonos and QuickBird imagery as well as images taken from Google Earth show that the proposed algorithm provides good localization of the target objects even when no sharp boundaries exist in the image data. © 2012 IEEE

Early diagnosis of pancreatic cancer: neutrophil gelatinase-associated lipocalin as a marker of pancreatic intraepithelial neoplasia

Pancreatic cancer is a highly lethal malignancy with a dismal 5-year survival of less than 5%. The scarcity of early biomarkers has considerably hindered our ability to launch preventive measures for this malignancy in a timely manner. Neutrophil gelatinase-associated lipocalin (NGAL), a 24-kDa glycoprotein, was reported to be upregulated nearly 27-fold in pancreatic cancer cells compared to normal ductal cells in a microarray analysis. Given the need for biomarkers in the early diagnosis of pancreatic cancer, we investigated the expression of NGAL in tissues with the objective of examining if NGAL immunostaining could be used to identify foci of pancreatic intraepithelial neoplasia, premalignant lesions preceding invasive cancer. To examine a possible correlation between NGAL expression and the degree of differentiation, we also analysed NGAL levels in pancreatic cancer cell lines with varying grades of differentiation. Although NGAL expression was strongly upregulated in pancreatic cancer, and moderately in pancreatitis, only a weak expression could be detected in the healthy pancreas. The average composite score for adenocarcinoma (4.26±2.44) was significantly higher than that for the normal pancreas (1.0) or pancreatitis (1.0) (P<0.0001). Further, although both well- and moderately differentiated pancreatic cancer were positive for NGAL, poorly differentiated adenocarcinoma was uniformly negative. Importantly, NGAL expression was detected as early as the PanIN-1 stage, suggesting that it could be a marker of the earliest premalignant changes in the pancreas. Further, we examined NGAL levels in serum samples. Serum NGAL levels were above the cutoff for healthy individuals in 94% of pancreatic cancer and 62.5% each of acute and chronic pancreatitis samples. However, the difference between NGAL levels in pancreatitis and pancreatic cancer was not significant. A ROC curve analysis revealed that ELISA for NGAL is fairly accurate in distinguishing pancreatic cancer from non-cancer cases (area under curve=0.75). In conclusion, NGAL is highly expressed in early dysplastic lesions in the pancreas, suggesting a possible role as an early diagnostic marker for pancreatic cancer. Further, serum NGAL measurement could be investigated as a possible biomarker in pancreatitis and pancreatic adenocarcinoma

Promoter DNA Methylation of Oncostatin M receptor-β as a Novel Diagnostic and Therapeutic Marker in Colon Cancer

In addition to genetic changes, the occurrence of epigenetic alterations is associated with accumulation of both genetic and epigenetic events that promote the development and progression of human cancer. Previously, we reported a set of candidate genes that comprise part of the emerging “cancer methylome”. In the present study, we first tested 23 candidate genes for promoter methylation in a small number of primary colon tumor tissues and controls. Based on these results, we then examined the methylation frequency of Oncostatin M receptor-β (OSMR) in a larger number of tissue and stool DNA samples collected from colon cancer patients and controls. We found that OSMR was frequently methylated in primary colon cancer tissues (80%, 80/100), but not in normal tissues (4%, 4/100). Methylation of OSMR was also detected in stool DNA from colorectal cancer patients (38%, 26/69) (cut-off in TaqMan-MSP, 4). Detection of other methylated markers in stool DNA improved sensitivity with little effect on specificity. Promoter methylation mediated silencing of OSMR in cell lines, and CRC cells with low OSMR expression were resistant to growth inhibition by Oncostatin M. Our data provide a biologic rationale for silencing of OSMR in colon cancer progression and highlight a new therapeutic target in this disease. Moreover, detection and quantification of OSMR promoter methylation in fecal DNA is a highly specific diagnostic biomarker for CRC

Role of DNA methylation in head and neck cancer

Head and neck cancer (HNC) is a heterogenous and complex entity including diverse anatomical sites and a variety of tumor types displaying unique characteristics and different etilogies. Both environmental and genetic factors play a role in the development of the disease, but the underlying mechanism is still far from clear. Previous studies suggest that alterations in the genes acting in cellular signal pathways may contribute to head and neck carcinogenesis. In cancer, DNA methylation patterns display specific aberrations even in the early and precancerous stages and may confer susceptibility to further genetic or epigenetic changes. Silencing of the genes by hypermethylation or induction of oncogenes by promoter hypomethylation are frequent mechanisms in different types of cancer and achieve increasing diagnostic and therapeutic importance since the changes are reversible. Therefore, methylation analysis may provide promising clinical applications, including the development of new biomarkers and prediction of the therapeutic response or prognosis. In this review, we aimed to analyze the available information indicating a role for the epigenetic changes in HNC

Development of a method for the implementation of CE tools in SME networks

Die Fähigkeit zur unternehmensübergreifenden Kooperation wird zukünftig verstärkt an Bedeutung gewinnen, da die Marktdynamik die Reaktionsfähigkeit einzelner Unternehmen übersteigen wird. Unternehmen werden sich verstärkt auf Kernkompetenzen konzentrieren und über Kooperationen die nachgefragten kompletten Problemlösungen realisieren. Als letzte Konsequenz dieser Entwicklung sind virtuelle Unternehmen zu sehen, in denen sich Unternehmen für nur einen Auftrag zusammenschließen und nach der Leistungserbringung auseinander gehen. Aus dieser sicht können die einzelne Firmen als Mitglieder eines Kooperationsnetzwerkes betrachtet werden, die geografisch verteilt sind, dennoch neue Werkzeuge und Methoden brauchen um ein lückenlose Zusammenarbeit leisten zu können

Surgical rehabilitation following ocular chemical injury

PubMedID: 23713679Purpose: The purpose of this study is to evaluate the management of limbal stem cell deficiency (LSCD) secondary to chemical ocular burns. Materials and methods: The charts of 48 eyes of 40 patients with grade 2 or higher chemical injury were evaluated retrospectively. Subjects with follow-up longer than 1 year were included. Medical treatment, surgical correction of abnormalities of ocular adnexial structures, limbal stem cell transplantation from patient's fellow eye, from living relatives or from cadaveric donor, amniotic membrane transplantation, conjunctival epitheliectomy, chelation with ethylenediaminetetraacetic acid and penetrating keratoplasty were the treatment modalities. Outcome measures were ocular surface stability and corrected distance visual acuity (CDVA). Failure was defined as the appearance of persistent epithelial defect (nonhealing epithelial defect for more than 2 weeks) with progressive corneal conjunctivalization/vascularization and thinning, and also progression of conjunctivalization to the central 6mm of the cornea in eyes with subsequent keratoplasty. Results: The mean age of 31 male and 9 female patients were 32.32±12.6 years. LSCD was bilateral in 8 cases. The mean follow-up was 77.2±35.1 months. The presentations were in acute phase in 37.5%, in subacute phase in 32.5% and in chronic phase in 30% of the patients. Only 13 of 48 (27.1%) eyes obtained sufficient ocular surface stability through medical treatment; however, only 5 of these eyes achieved CDVA of less than 0.7 logMAR. Limbal stem cell transplantation was performed in 26 eyes as conjunctival limbal autograft, living-related conjunctival limbal allograft and keratolimbal allograft or as a combination of these transplantations. At the last visit, 30 eyes (62.5%) had an intact and stable ocular surface. Clear cornea was achieved in 11 (78.6%) of 14 eyes with grade 2 injury, in 9 (60%) of 15 eyes with grade 3 injury, in 5 (50%) of 10 eyes with grade 4 injury, in 1 (16.6%) of 6 eyes with grade 5 injury and in 1 (33.3%) of 3 eyes with grade 6 injury. The CDVA that was 1.66±0.99 logMAR initially improved to 0.87±0.85 logMAR at the last visit (p<0.001). Conclusion: While patients with low-grade chemical injury seem to benefit quite well from the medical treatment, amniotic membrane transplantation, limbal graft transplantation and subsequent keratoplasty; patients with severe injuries seem to be more prone to failure after all of the available treatment modalities. © 2014 Informa Healthcare USA, Inc