Study of fetomaternal outcome in cases of placenta previa at tertiary care hospital

Background: Placenta previa is defined as placenta implanted partially or completely in the lower uterine segment. It contributes upto 30% of the cases the antepartum hemorrhage. This catastrophic complication not only poses a risk to the fetus but also endangers the mother’s life. The objective of the study was to determine the incidence and risk factors, obstetric management, maternal mortality and morbidity and perinatal outcome in women presenting with placenta previa.Methods: It is the prospective study of 50 cases carried out to study the maternal and perinatal outcome in cases of placenta previa in tertiary care hospital. This study included antenatal patient diagnosed as placenta previa by ultrasound >28 weeks to full term pregnancy. This data was compiled and analyzed for maternal and neonatal outcome.Results: In the present study, the incidence of placenta previa is 0.8% among which 42% of cases having age group of 25-29 years and 72% cases are multigravida. In this study 60% cases have major degree of placenta previa and 66% cases have previous history of caesarean section. Out of total cases 96% cases delivered by caesarean section and 4% had Normal delivery. NICU admission in the study is 28 babies i.e. 56%. There is no maternal mortality seen in the present study.Conclusions: Risk factors that increase the cases of placenta previa are multiparity, previous caesarean section, previous abortion. Placenta previa is major risk factor for adverse maternal and perinatal outcome. Good antenatal care, availability of emergency obstetrics services with senior obstetricians, blood bank facility, ICU care and NICU services can improve maternal and neonatal outcome in high risk cases

Immunomagnetic microbeads for screening with flow cytometry and identification with nano-liquid chromatography mass spectrometry of ochratoxins in wheat and cereal

Multi-analyte binding assays for rapid screening of food contaminants require mass spectrometric identification of compound(s) in suspect samples. An optimal combination is obtained when the same bioreagents are used in both methods; moreover, miniaturisation is important because of the high costs of bioreagents. A concept is demonstrated using superparamagnetic microbeads coated with monoclonal antibodies (Mabs) in a novel direct inhibition flow cytometric immunoassay (FCIA) plus immunoaffinity isolation prior to identification by nano-liquid chromatography–quadrupole time-of-flight-mass spectrometry (nano-LC-Q-ToF-MS). As a model system, the mycotoxin ochratoxin A (OTA) and cross-reacting mycotoxin analogues were analysed in wheat and cereal samples, after a simple extraction, using the FCIA with anti-OTA Mabs. The limit of detection for OTA was 0.15 ng/g, which is far below the lowest maximum level of 3 ng/g established by the European Union. In the immunomagnetic isolation method, a 350-times-higher amount of beads was used to trap ochratoxins from sample extracts. Following a wash step, bound ochratoxins were dissociated from the Mabs using a small volume of acidified acetonitrile/water (2/8 v/v) prior to separation plus identification with nano-LC-Q-ToF-MS. In screened suspect naturally contaminated samples, OTA and its non-chlorinated analogue ochratoxin B were successfully identified by full scan accurate mass spectrometry as a proof of concept for identification of unknown but cross-reacting emerging mycotoxins. Due to the miniaturisation and bioaffinity isolation, this concept might be applicable for the use of other and more expensive bioreagents such as transport proteins and receptors for screening and identification of known and unknown (or masked) emerging food contaminants

Advancement in the field of magnetic fluids for drug discovery, medicine and biotechnology

358-362Techniques based on magnetic separation have become increasingly popular among the biological community. Magnetic separation technique offers advantage in terms of subjecting, analyte to very little mechanical stress compared to other methods, they are rapid, often highly scalable, low cost and avoid use of hazardous toxic reagents. Techniques employing magnetism are more amenable to automation and miniaturization. This paper reviews the advancement made in the field of medicine, biotechnology and drug discovery using magnetic particles technology

Immobilization of enzymes on magnetic particles

Item not available in this repository.Magnetic particles have been increasingly used as carriers for binding proteins, enzymes, and drugs. Such immobilization procedures for proteins, enzymes, antibodies, and other biologically active compounds have a major impact in different areas of biomedicine and biotechnology. The immobilized biomolecules can be used directly for a bioassay or as affinity ligands to capture or modify target molecules or cells. This chapter details immobilization procedures for proteins and enzymes onto various magnetically responsive carriers such as naked magnetic particles, carboxyl-modified microspheres, and aminomodified microspheres using direct binding procedure in the presence of coupling agents such as carbodiimide. The physical and chemical properties of freshly prepared magnetic particles were determined by magnetic measurements (VSM magnetometer), transmission electron microscopy (TEM), and atomic force microscopy (AFM). The extent of immobilization and enzyme activities were spectrophotometrically measured in order to find the retained activity after immobilization onto magnetic particles. The binding of proteins and enzymes was also confirmed by TEM microscopy.https://doi.org/10.1007/978-1-59745-053-9_19pubpu

EERI Virtual Earthquake Reconnaissance Team (VERT): Phase 1 Response to M5.7 Magna Earthquake

The Earthquake Engineering Research Institute (EERI) Virtual Earthquake Reconnaissance Team (VERT) is a subcommittee of the Learning from Earthquakes Committee. VERT performs virtual reconnaissance after an earthquake to provide timely multi-disciplinary information to the reconnaissance community to assist in decisions being made after an event. This document is the Phase 1 report for the Magna Earthquake