Nefropatía congénita en Schnauzer miniatura

Un Schnauzer miniatura, macho, de 13 meses de edad, presenta un cuadro de vómitos crónicos con historia clínica de gastritis. El examen físico y las pruebas complementarias permiten diagnosticar un cuadro compatible con la nefropatía familiar del Schnauzer miniatura. Se instauró dieta y tratamiento médico hasta que el animal empeoró a los dos meses y medio

Indicadores para la selección de talentos en el Atletismo, área de velocidad (vallas), categoría 13-14 años (Original).

Witch the results of this research, the selection system for sprinter talents velocity in Athletics is perfected, specifically in the hurdlers of the 13and 14 years old category in particular, its stages 2 and 3 corresponding to the entry into the school sports initiation school. Adaptations are proposed from the identification of more specific indicators in terms of their relationship with the particularities of the competitive of this discipline, which are locate by the nature in the technical and physical motor dimensions. Methods and research techniques of the theoretical, empirical and statistical levels were used that allowed to base the object and field of the investigation, determine the regularities from practice and theory, as well as process the data obtained. The need for these indicators is based on the diagnosis where is revealed, among other, that there are no dimensions or indicators by which by the speed race in the hundlers of the 13-14 years old category can be evaluated, it is in the interest of the national commission that document be written that can say how to determine a possible talent in the different disciplines that are related to the etalons of the 13-14 years old category, this category does not have a table where indicators appear to measure the performance of the athletes. The results achieved reveal the feasibility of improving the technical and physical motor indicator of speed races in short distance hurdlers of the 13-14 years old category.Con los resultados de esta investigación se perfecciona el sistema de selección de talentos velocistas en el Atletismo, específicamente para las carreras con vallas, categoría 13-14 años. Se elaboran indicadores específicos a partir de las exigencias de la disciplina y las particularidades de la actividad competitiva, que se ubican por su naturaleza en las dimensiones técnicas y físico-motrices. Se utilizaron métodos y técnicas de investigación de los niveles teóricos, empíricos y estadísticos que permitieron fundamentar el objeto y campo de la investigación, determinar las regularidades desde la práctica y la teoría; así como procesar los datos obtenidos. La necesidad de la investigación se sustenta a partir del diagnóstico, donde se revela la carencia de indicaciones específicas para favorecer la selección de futuro vallista de distancias cortas, categoría 13-14 años. Además es de interés para la comisión nacional de Atletismo, que se profundice en la elaboración de instrumentos, herramientas y formas para determinar un posible talento en la disciplina motivo de estudio, pues no cuenta con las normas para medir el rendimiento de los vallistas, así como otras herramientas, que le permitan evaluar la calidad de la ejecución técnica en estas edades. Los resultados alcanzados en la valoración teórica de la propuesta develan la funcionabilidad para su aplicación durante el proceso de selección de talento del velocista-vallista en la categoría 13-14 años

Sea Bass Primary Cultures versus RTgill-W1 Cell Line: Influence of Cell Model on the Sensitivity to Nanoparticles

Determination of acute toxicity to vertebrates in aquatic environments is mainly performed following OECD test guideline 203, requiring the use of a large number of fish and with mortality as endpoint. This test is also used to determine toxicity of nanomaterials in aquatic environments. Since a replacement method for animal testing in nanotoxicity studies is desirable, the feasibility of fish primary cultures or cell lines as a model for nanotoxicity screenings is investigated here. Dicentrarchus labrax primary cultures and RTgill-W1 cell line were exposed to several concentrations (0.1 to 200 ug/mL) of different nanoparticles (TiO2, polystyrene and silver), and cytotoxicity, metabolic activity and reactive oxygen species formation were investigated after 24 and 48 h of exposure. Protein corona as amount of protein bound, as well as the influence of surface modification (-COOH, -NH2), exposure media (Leibovitz’s L15 or seawater), weathering and cell type were the experimental variables included to test their influence on the results of the assays. Data from all scenarios was split based on the significance each experimental variable had in the result of the cytotoxicity tests, in an exploratory approach that allows for better understanding of the determining factors affecting toxicity. Data shows that more variables significantly influenced the outcome of toxicity tests when the primary cultures were exposed to the different nanoparticles. Toxicity tests performed in RTgill-W1 were influenced only by exposure time and nanoparticle concentration. The whole data set was integrated in a biological response index to show the overall impact of nanoparticle exposures.This study was supported by a postdoctoral grant to AJ-R (Basque Government; POSDOC program 2017–2019), Basque Country, Spain; and Federal Ministry of Education and Research, BMBF (NanoUmwelt, grant agreement Nº 030150B), Germany

Barrett’s esophagus registries

The following on Barrett's esophagus registries contains commentaries on the data sets to be included, organizational issues, and the demographic, lifestyle, and diagnostic differences between the United States and Europe. The importance of collaborative studies is also discussed

Análisis comparativo de subtítulos profesionales y fansubs en un capítulo de la serie “Riverdale”

La presente investigación tuvo como objetivo principal analizar los subtítulos profesionales y los fansubs, del primer capítulo de la primera temporada de la serie Riverdale. Para esto, se creó una ficha de análisis como instrumento, con la cual, se observó y analizó la muestra seleccionada. Este trabajo se basó en investigaciones de diferentes autores que se relacionaban con las variables, subtitulación profesional y fansub. Esta investigación tiene un enfoque cuantitativo, es un estudio descriptivo comparativo. Por lo tanto, se analizaron 200 subtítulos profesionales y 200 fansubs, ambos extraídos del primer capítulo de la primera temporada de la serie Riverdale. Se analizaron de acuerdo a 3 dimensiones: formato del subtítulo, convenciones ortotipográficas y la velocidad de lectura, teniendo en cuenta indicadores como: tipo de letra Arial, número de caracteres, posición en la pantalla, segmentación, ortografía, puntuación y caracteres por segundo (CPS). Se encontró que, en relación al formato del subtítulo, el fansub tuvo más errores que el subtítulo profesional especialmente en lo que refiere a la segmentación mientras que el subtítulo profesional solo tiene errores en el número de caracteres. Asimismo, en cuanto a las convenciones ortotipográficas, en el fansub se encontró errores en la puntuación y la ortografía. Además, el fansub se excedió en cuanto al número de caracteres permitidos para la velocidad de lectura, mientras que Netflix solo tuvo dos errores en este indicador

Prevalence of clinical forms of Chagas disease: a systematic review and meta-analysis – data from the RAISE study

Chagas disease; PrevalenceEnfermedad de Chagas; PrevalenciaMalaltia de Chagas; PrevalençaBackground There is a lack of up-to-date estimates about the prevalence of Chagas disease (ChD) clinical presentations and, therefore, we aimed to assess the prevalence of clinical forms of ChD among seropositive adults, pooling available data. Methods A systematic review was conducted in Medline, Embase, Biblioteca Virtual em Saúde and Cochrane databases looking for studies published from 1990 to August 2023, which investigated the prevalence of ChD clinical forms among seropositive adults, including: (i) indeterminate phase, (ii) chronic Chagas cardiomyopathy (CCM), (iii) digestive and (iv) mixed (CCM + digestive) forms. Pooled estimates and 95% confidence intervals (CI) were calculated using random-effects models. Studies quality and risk of bias was assessed with the Leboeuf-Yde and Lauritsen tool. Heterogeneity was assessed with the I2 statistic. The study was registered in the PROSPERO database (CRD42022354237). Findings 1246 articles were selected for screening and 73 studies were included in the final analysis (17,132 patients, 44% men). Most studies were conducted with outpatients (n = 50), followed by population-based studies (n = 15). The pooled prevalence of the ChD clinical forms was: indeterminate 42.6% (95% CI: 36.9–48.6), CCM 42.7% (95% CI: 37.3–48.3), digestive 17.7% (95% CI: 14.9–20.9), and mixed 10.2% (95% CI: 7.9–13.2). In population-based studies, prevalence was lower for CCM (31.2%, 95% CI: 24.4–38.9) and higher for indeterminate (47.2%, 95% CI: 39.0–55.5) form. In meta-regression, age was inversely associated with the prevalence of indeterminate (β = −0.05, P < 0.001) form, and directly associated with CCM (β = 0.06, P < 0.001) and digestive (β = 0.02, P < 0.001) forms. Heterogeneity was overall high. Interpretation Compared to previous publications, our pooled estimates show a higher prevalence of CCM among ChD seropositive patients, but similar rates of the digestive form.This study was funded by Novartis Pharma AG as part of a research collaboration with the World Heart Federation, project number CLCZ696D2010R. The World Heart Federation funded the Federal University of Minas Gerais to lead the study. Dr Pinho Ribeiro is supported in part by CNPq (310790/2021-2 and 465518/2014-1). Dr. Ramos Nascimento is supported in part by CNPq (Bolsa de produtividade em pesquisa, 310749/2022-0), by the Edwards Lifesciences Foundation (Improving the Prevention and Detection of Heart Valve Disease Across the Lifespan, 2023) and by FAPEMIG (grant APQ-000627-20)

Prevalence of Chagas disease among Latin American immigrants in non-endemic countries: an updated systematic review and meta-analysis

Chagas disease; Latin american migrants; PrevalenceEnfermedad de Chagas; Migrantes latinoamericanos; PrevalenciaMalaltia de Chagas; Migrants llatinoamericans; PrevalènciaBackground Chagas disease (CD), endemic in 21 Latin American countries, has gradually spread beyond its traditional borders due to migratory movements and emerging as a global health concern. We conducted a systematic review and meta-analysis of available data to establish updated prevalence estimates of CD in Latin American migrants residing in non-endemic countries. Methods A systematic search was conducted in MEDLINE/PubMed, Embase, Cochrane Library, Scopus, Web of Science, and LILACS via Virtual Health Library (Biblioteca Virtual em Saúde - BVS), including references published until November 1st, 2023. Pooled prevalence estimates and 95% confidence intervals (CI) were calculated using random effect models. Heterogeneity was assessed by the chi-square test and the I2 statistic. Subgroup analyses were performed to explore potential sources of heterogeneity among studies. The study was registered in the PROSPERO database (CRD42022354237). Findings From a total of 1474 articles screened, 51 studies were included. Studies were conducted in eight non-endemic countries (most in Spain), between 2006 and 2023, and involving 82,369 screened individuals. The estimated pooled prevalence of CD in Latin American migrants living in non-endemic countries was 3.5% (95% CI: 2.5–4.7; I2: 97.7%), considering studies in which screening was indicated simply because the person was Latin American. Per subgroups, the pooled CD prevalence was 11.0% (95% CI: 7.7–15.5) in non-targeted screening (unselected population in reference centers) (27 studies); in blood donors (4 studies), the pooled prevalence was 0.8% (95% CI: 0.2–3.4); among people living with HIV Latin American immigrants (4 studies) 2.4% (95% CI: 1.4–4.3) and for Latin American pregnant and postpartum women (14 studies) 3.7% (95 CI: 2.4–5.6). The pooled proportion of congenital transmission was 4.4% (95% CI: 3.3–5.8). Regarding the participants’ country of origin, 7964 were from Bolivia, of which 1715 (21,5%) were diagnosed with CD, and 21,304 were from other Latin American countries of which 154 (0,72%) were affected. Interpretation CD poses a significant burden of disease in Latin American immigrants in non-endemic countries, suggesting that CD is no longer a problem limited to the American continent and must be considered as a global health challenge.This study was funded by the World Heart Federation, through a research collaboration with Novartis Pharma AG

Assessment of iron oxide nanoparticle ecotoxicity on regeneration and homeostasis in the replacement model system Schmidtea mediterranea

Iron oxide nanoparticles (IONs) are used in a number of applications, from food to cosmetics, from medical applications to magnetic storage. In spite of the 550 tons produced each year in Europe alone, no effective dose limit recommendations are established and the overall risks connected to IONs are still debated. The incorporation of IONs in daily life raises a concern about their effects on the environment, on living organisms, and on human health. In this study, we used freshwater planarians to assess the nanoecotoxicity of IONs. Planarians are free-living invertebrates known for their astonishing regenerative ability. Because of their sensitivity to toxicants, they are often used to determine the effects of toxic, genotoxic and carcinogenic environmental compounds with an approach in line with the 3Rs (Reduce, Refine, Replace) principle. Planarians were exposed to IONs at concentrations up to 1 mg/mL and their effects were evaluated at the behavioral, morphofunctional and molecular levels, with a special emphasis on the regeneration process. Our results indicate that IONs did not affect the stem cell population dynamics, nor did they induce substantial changes in either homeostatic or regenerating planarians. As positive controls, gold nanoparticles coated with the pro-apoptotic anti-cancer drug hexadecylmethylammonium bromide, silver nanoparticles and highly concentrated polystyrene nanoparticles were used. These all elicited toxic effects. Therefore, we conclude that IONs at environmental concentrations are safe for planarians, and that the planarian is a powerful model system that can replace vertebrate animal models in nanoecotoxicology research and for nanoecotoxicology studies