Transcriptional activation of CBFβ by CDK11p110 is necessary to promote osteosarcoma cell proliferation.

BACKGROUND:Aberrant expression of cyclin-dependent protein kinases (CDK) is a hallmark of cancer. CDK11 plays a crucial role in cancer cell growth and proliferation. However, the molecular mechanisms of CDK11 and CDK11 transcriptionally regulated genes are largely unknown. METHODS:In this study, we performed a global transcriptional analysis using gene array technology to investigate the transcriptional role of CDK11 in osteosarcoma. The promoter luciferase assay, chromatin immunoprecipitation assay, and Gel Shift assay were used to identify direct transcriptional targets of CDK11. Clinical relevance and function of core-binding factor subunit beta (CBFβ) were further accessed in osteosarcoma. RESULTS:We identified a transcriptional role of protein-DNA interaction for CDK11p110, but not CDK11p58, in the regulation of CBFβ expression in osteosarcoma cells. The CBFβ promoter luciferase assay, chromatin immunoprecipitation assay, and Gel Shift assay confirmed that CBFβ is a direct transcriptional target of CDK11. High expression of CBFβ is associated with poor outcome in osteosarcoma patients. Expression of CBFβ contributes to the proliferation and metastatic behavior of osteosarcoma cells. CONCLUSIONS:These data establish CBFβ as a mediator of CDK11p110 dependent oncogenesis and suggest that targeting the CDK11- CBFβ pathway may be a promising therapeutic strategy for osteosarcoma treatment

Research on Product Conceptual Design Based on Integrated of TRIZ and HOQ

Abstract. The conceptual design determines the success of the final product quality and competition of market. The determination of design parameters and the effective method to resolve parameters contradiction are the key to success. In this paper, the concept of HOQ products designed to determine the parameters, then using the TRIZ contradiction matrix and inventive principles of design parameters to solve the problem of contradictions. Facts have proved that the effective method is to obtain the product concept design parameters and to resolve contradictions line parameters

The Analysis of Innovation Policies for New Energy Vehicle Technology

Based on Yu ling Liu’s innovation policy analysis framework, this paper sorted the introduction of innovation policies for new energy vehicles in various periods in China and comparatively analyzes the domestic and foreign policy, which led to the discovery of “three similarities and three differences”.  Also, it raised the idea of strategic adjustment for new energy vehicle technology innovation, providing a reference for decision-making to further promote the new energy vehicle technology progress and industrialization

The Antecedents Of Process Innovation And Smes Growth: Empirical Evidence From Shoe Manufacturing Sector In The Ashanti Region Of Ghana

This article contributes to the study of process innovation as a growth strategy for SMEs, enriching and complementing the widely held view that innovation in general impacts positively on SMEs productivity and growth. In furtherance, this study looks at the process innovation from three main angles as proposed by the Oslo Manuel, which are new process, improved process, and new and improved distribution. Firm level data were purposively collected from shoes manufacturing companies located in the Ashanti Region of Ghana, where Structural Equation Model were employed in coming out with the path estimates in determining the relationship between the variables. The results indicated that, adoption of new and improved distribution strategy impact positively on the growth of the SMEs, by way of reducing the cost of operations and increases customers’ satisfaction. This was followed by the adoption of improved process strategy, where the productivity saw a massive improvement in the areas of quality and quantity, but there was no significance in the reduction of cost of production, whereas the adoption of new process strategy saw an increase in production, but its associated high cost of production negated the growth, to impact negatively on the growth of the SMEs. Base on the results from the study, our results suggest that adoption of process innovation have significant effect on the performance of the shoes manufacturing, the positive impact is not a wholesale, therefore entrepreneurs should not adopt all process innovation blanketly

Study of New Wall Materials Design Based on TRIZ Integrated Innovation Method

New wall materials alleviate the problems of high energy consumption and heavy pollution in the production process through the forms of new natural raw materials, energy conservation, land conservation, waste utilization, etc.. In this paper, design of new building wall materials is achieved through the integrated innovation method of Theory of Inventive Problem Solving (TRIZ), Technology Acceptance Model (TAM), and Quality Function Deployment (QFD). Technical contradictions and physical contradictions in various stages of product design and production are resolved from the perspectives of user survey, R & D design, manufacturing, marketing. According to the different advantages of TRIZ, TAM and QFD in various stages of product, new wall material products of Guizhou Long Life Forestry Group are used as an example, with the integrated innovation method, the company’s new wall materials products are designed, and green, environmental, economical series wall materials products are designed and marketed in China.Key words: TRIZ; TAM; QFD; New building materials; Integrated innovation metho

Correction to: Transcriptional activation of CBFβ by CDK11p110 is necessary to promote osteosarcoma cell proliferation.

Following publication of the original article [1], it was reported that Figs. 4 and 5 were not updated during the production process

The influence of adjunctive traditional Chinese medicine therapy on survival in primary liver cancer: a real-world study based on electronic medical records

Background: Traditional Chinese medicine (TCM) effectively improves the survival rate and quality of life of primary liver cancer patients, but high-level evidence is lacking.Patients and methods: Patients were selected from 5 tertiary hospitals in Henan Province, China. Two thousand sixty-seven patients with primary liver cancer were included in the study. The electronic medical records (EMRs) of the patients were collected. Patients who received adjunctive TCM treatment and underwent treatment cumulative time for more than 1 month were classified as the TCM intervention cohort. Patients who did not receive adjunctive TCM treatment or underwent treatment cumulative time for less than 1 month were classified as the non-TCM intervention cohort. The main outcome indicators were the survival rate and overall survival time. The propensity score inverse probability weighting method was used to balance the differences between the groups.Results: The primary cohort comprised 2,067 patients, including 462 patients who received adjunctive TCM treatment and 1,605 patients who did not receive adjunctive TCM treatment. The results of the Kaplan‒Meier survival curve indicated that the survival rate and median survival time of the exposure group before and after propensity score weighting were greater than those of the control group (p < 0.0001). Univariate Cox regression analysis after propensity score weighting showed that adjunctive TCM treatment was an independent protective factor for survival [regression coefficient = −0.215, hazard ratio (HR) = 0.8066, 95% confidence interval (CI) (0.6609–0.9844)].Conclusion: Adjuvant treatment with TCM has a protective effect on the prognosis of patients with primary liver cancer; it can reduce the mortality and prolong the survival time