Study on bearing mechanical and thermal characteristic evolvement rules affected by higher ambient temperature

In this paper, the bearing mechanical characteristics and thermal characteristics as it is running under constant speed (2400 r/min) and constant temperature (100 ℃) adopting bearing mechanical-thermal coupling model built on the basis of quasi-statics and elastohydrodynamic lubrication theory are discussed. Among which, the contact deformation between ball and inner/outer ring has same evolution law, besides, the same as the amplitude. Whose curve shape changes in the form of “circular-oblique D”, its amplitude rearches minimum value at 10thd3h between the location of 120° and 270°. The curve shapes belonging to contact angular between ball and inner/outer ring are similar to “circular”. There is opposite trend of them within the range of 90-300°. The amplitude of contact angular between ball and outer ring is the minimum at 10thd3h and 11thd1h. At the same time, the amplitude of that between ball and inner ring is the maximum. The evolution rules of contact stiffness between ball and inner/outer ring are generally consistent, which are similar to “crab”. But the amplitude of that between ball and outer ring is dominant. The maximum value of them occurs at 10thd3 and 11thd1h. The evolvement tendency of node temperatures maintains stable. Thereinto, the temperature of inner ring equals to that of contact location between ball and inner ring, which is the maximum value. It is obvious that ME, MD, MS, MCB, MCR and MOil devote themselves to heat production, the contribution rates of ME, MS, MCB and MCR are 100 %, the contribution rate of MD ranges from 52.6 % to 65.2 %. However, the amplitudes of friction moments have the opposite trend compared to “heat contribution factor”. The expansion amount owing to heat production is dominant in displacement variation and the effect of clearance on displacement can be ignored, whose evolution rules are contrary. The evolution rules of oil film thickness and oil film stiffness between ball and inner/outer ring are alike. Among which, the oil film thickness between ball and outer ring is dominant, its maximum value emerges at 10thd3h. The curve shapes of oil film stiffness between ball and outer/inner ring are oblique “D”, their amplitudes reach the maximum at 11thd1h-11thd3h

Single-cell RNA sequencing reveals in vivo osteoimmunology interactions between the immune and skeletal systems

BackgroundWhile osteoimmunology interactions between the immune and skeletal systems are known to play an important role in osteoblast development, differentiation and bone metabolism related disease like osteoporosis, such interactions in either bone microenvironment or peripheral circulation in vivo at the single-cell resolution have not yet been characterized.MethodsWe explored the osteoimmunology communications between immune cells and osteoblastic lineage cells (OBCs) by performing CellphoneDB and CellChat analyses with single-cell RNA sequencing (scRNA-seq) data from human femoral head. We also explored the osteoimmunology effects of immune cells in peripheral circulation on skeletal phenotypes. We used a scRNA-seq dataset of peripheral blood monocytes (PBMs) to perform deconvolution analysis. Then weighted gene co-expression network analysis (WGCNA) was used to identify monocyte subtype-specific subnetworks. We next used cell-specific network (CSN) and the least absolute shrinkage and selection operator (LASSO) to analyze the correlation of a gene subnetwork identified by WGCNA with bone mineral density (BMD).ResultsWe constructed immune cell and OBC communication networks and further identified L-R genes, such as JAG1 and NOTCH1/2, with ossification related functions. We also found a Mono4 related subnetwork that may relate to BMD variation in both older males and postmenopausal female subjects.ConclusionsThis is the first study to identify numerous ligand-receptor pairs that likely mediate signals between immune cells and osteoblastic lineage cells. This establishes a foundation to reveal advanced and in-depth osteoimmunology interactions to better understand the relationship between local bone microenvironment and immune cells in peripheral blood and the impact on bone phenotypes

Enzyme Stability and Activity in Non-Aqueous Reaction Systems: A Mini Review

Enormous interest in biocatalysis in non-aqueous phase has recently been triggered due to the merits of good enantioselectivity, reverse thermodynamic equilibrium, and no water-dependent side reactions. It has been demonstrated that enzyme has high activity and stability in non-aqueous media, and the variation of enzyme activity is attributed to its conformational modifications. This review comprehensively addresses the stability and activity of the intact enzymes in various non-aqueous systems, such as organic solvents, ionic liquids, sub-/super-critical fluids and their combined mixtures. It has been revealed that critical factors such as Log P, functional groups and the molecular structures of the solvents define the microenvironment surrounding the enzyme molecule and affect enzyme tertiary and secondary structure, influencing enzyme catalytic properties. Therefore, it is of high importance for biocatalysis in non-aqueous media to elucidate the links between the microenvironment surrounding enzyme surface and its stability and activity. In fact, a better understanding of the correlation between different non-aqueous environments and enzyme structure, stability and activity can contribute to identifying the most suitable reaction medium for a given biotransformation

Mode-II interlaminar fracture toughness of GFRP/Al laminates improved by surface modified VGCF interleaves

Mode-II interlaminar fracture toughness of GFRP/Al (aluminum) laminates is improved by employing acid treatment to an Al plate and inserting surface modified vapor grown carbon fiber (S-VGCF) between the Al plate and a GFRP layer. End notched flexure (ENF) tests are carried out to investigate the effect of S-VGCF addition on the Mode-II interlaminar fracture toughness. The experimental results demonstrate that the critical load and Mode-II fracture toughness of the specimen with acid treatment of Al plate and 10 g/m2 addition of surface modified VGCF are enhanced by 135.51% and 425.16%, respectively, compared to those of corresponding pristine specimen. Due to the presence of the oxygen containing functional groups, the specimens with addition of S-VGCF possess much higher Mode-II fracture toughness compared to the specimens with addition of pristine VGCF. Laser scanning and scanning electron microscopies are employed to observe the fracture surface of specimens to reveal the improvement mechanisms. Moreover, numerical simulations are performed based on the cohesive zone model to verify the experimental results. The interfacial shear strength between the Al plate and the GFRP layer are also predicted by the numerical simulations