926 research outputs found
Chlorophyll dephytylase 1 and chlorophyll synthase: a chlorophyll salvage pathway for the turnover of photosystems I and II
Chlorophyll (Chl) is made up of the tetrapyrrole chlorophyllide and phytol, a diterpenoid alcohol. The photosynthetic protein complexes utilize Chl for light harvesting to produce biochemical energy for plant development. However, excess light and adverse environmental conditions facilitate generation of reactive oxygen species, which damage photosystems I and II (PSI and PSII) and induce their turnover. During this process, Chl is released, and is thought to be recycled via dephytylation and rephytylation. We previously demonstrated that Chl recycling in Arabidopsis under heat stress is mediated by the enzymes chlorophyll dephytylase 1 (CLD1) and chlorophyll synthase (CHLG) using chlg and cld1 mutants. Here, we show that the mutants with high CLD1/CHLG ratio, by different combinations of chlgâ1 (a knockâdown mutant) and the hyperactive cld1â1 alleles, develop necrotic leaves when grown under longâ and shortâday, but not continuous light conditions, owing to the accumulation of chlorophyllide in the dark. Combination of chlgâ1 with cld1â4 (a knockâout mutant) leads to reduced chlorophyllide accumulation and necrosis. The operation of CLD1 and CHLG as a Chl salvage pathway was also explored in the context of Chl recycling during the turnover of Chlâbinding proteins of the two photosystems. CLD1 was found to interact with CHLG and the lightâharvesting complexâlike proteins OHP1 and LIL3, implying that auxiliary factors are required for this process.Deutsche Forschungsgemeinschaft
http://dx.doi.org/10.13039/501100001659Ministry of Science and Technology, Taiwan
http://dx.doi.org/10.13039/501100004663Peer Reviewe
Muckenhoupt-type weights and the intrinsic structure in Bessel Setting
Fix and . Consider the Bessel operator
(introduced by Muckenhoupt--Stein)
on
with and the
Lebesgue measure on . In this paper, we study the
Muckenhoupt-type weights which reveal the intrinsic structure in this Bessel
setting along the line of Muckenhoupt--Stein and Andersen--Kerman. Besides,
exploiting more properties of the weights introduced by
Andersen--Kerman, we introduce a new class such
that the Hardy--Littlewood maximal function is bounded on the weighted
space if and only if is in . Moreover, along the
line of Coifman--Rochberg--Weiss, we investigate the commutator
with to be the
Bessel Riesz transform. We show that for , the commutator
is bounded on weighted if and only if is in the
BMO space associated with .Comment: 30 page
Graphlet and Orbit Computation on Heterogeneous Graphs
Many applications, ranging from natural to social sciences, rely on graphlet
analysis for the intuitive and meaningful characterization of networks
employing micro-level structures as building blocks. However, it has not been
thoroughly explored in heterogeneous graphs, which comprise various types of
nodes and edges. Finding graphlets and orbits for heterogeneous graphs is
difficult because of the heterogeneity and abundance of semantic information.
We consider heterogeneous graphs, which can be treated as colored graphs. By
applying the canonical label technique, we determine the graph isomorphism
problem with multiple states on nodes and edges. With minimal parameters, we
build all non-isomorphic graphs and associated orbits. We provide a Python
package that can be used to generate orbits for colored directed graphs and
determine the frequency of orbit occurrence. Finally, we provide four examples
to illustrate the use of the Python package.Comment: 13 pages, 7 figure
Refractory gastric variceal bleeding secondary to splenic vein occlusion associated with abdominal lymphadenopathy
SummarySplenic vein occlusion caused by abdominal lymphadenopathy is rare. We herein present the case of a 80-year-old man with refractory isolated gastric variceal bleeding in the absence of pancreatic or liver disease. Left-sided portal hypertension was confirmed by angiography, and para-aortic lymphadenopathy compressing the splenic vein was identified by serial abdominal computed tomography. Endoscopic sclerosing therapy failed to treat the recurring gastric variceal hemorrhage. Therefore, splenectomy was suggested and the patient was successfully treated. The patient had been variceal bleeding free for 12 months since the surgery. In patients with isolated gastric varices but without advanced liver disease, a variety of diagnostic techniques should be attempted to elucidate the nature of portal hypertension, and left-sided portal hypertension should be suspected. For those cases in which endoscopic treatment failed to treat refractory gastric variceal bleeding, splenectomy can be an effective option
ECG Signal Super-resolution by Considering Reconstruction and Cardiac Arrhythmias Classification Loss
With recent advances in deep learning algorithms, computer-assisted
healthcare services have rapidly grown, especially for those that combine with
mobile devices. Such a combination enables wearable and portable services for
continuous measurements and facilitates real-time disease alarm based on
physiological signals, e.g., cardiac arrhythmias (CAs) from electrocardiography
(ECG). However, long-term and continuous monitoring confronts challenges
arising from limitations of batteries, and the transmission bandwidth of
devices. Therefore, identifying an effective way to improve ECG data
transmission and storage efficiency has become an emerging topic. In this
study, we proposed a deep-learning-based ECG signal super-resolution framework
(termed ESRNet) to recover compressed ECG signals by considering the joint
effect of signal reconstruction and CA classification accuracies. In our
experiments, we downsampled the ECG signals from the CPSC 2018 dataset and
subsequently evaluated the super-resolution performance by both reconstruction
errors and classification accuracies. Experimental results showed that the
proposed ESRNet framework can well reconstruct ECG signals from the 10-times
compressed ones. Moreover, approximately half of the CA recognition accuracies
were maintained within the ECG signals recovered by the ESRNet. The promising
results confirm that the proposed ESRNet framework can be suitably used as a
front-end process to reconstruct compressed ECG signals in real-world CA
recognition scenarios
Pulsed Wave Doppler Ultrasound Is Useful to Assess Vasomotor Response in Patients with Multiple System Atrophy and Well Correlated with Tilt Table Study
The study aim was to assess sympathetic vasomotor response (SVR) by using pulsed wave Doppler (PWD) ultrasound in patients with multiple system atrophy (MSA) and correlate with the tilt table study. We recruited 18 male patients and 10 healthy men as controls. The SVR of the radial artery was evaluated by PWD, using inspiratory cough as a provocative maneuver. The response to head-up tilt was studied by a tilt table with simultaneous heart rate and blood pressure recording. The hemodynamic variables were compared between groups, and were examined by correlation analysis. Regarding SVR, MSA patients exhibited a prolonged latency and less heart rate acceleration following inspiratory cough. Compared with the tilt table test, the elevation of heart rate upon SVR was positively correlated to the increase of heart rate after head-up tilt. The correlation analysis indicated that the magnitude of blood pressure drop from supine to upright was positively associated with the SVR latency but negatively correlated with the heart rate changes upon SVR. The present study demonstrated that blunted heart rate response might explain MSA's vulnerability to postural challenge. PWD may be used to predict cardiovascular response to orthostatic stress upon head-up tilt in MSA patients
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Functional Organization of a Neural Network for Aversive Olfactory Learning in Caenorhabditis elegans
Many animals use their olfactory systems to learn to avoid dangers, but how neural circuits encode naive and learned olfactory preferences, and switch between those preferences, is poorly understood. Here, we map an olfactory network, from sensory input to motor output, which regulates the learned olfactory aversion of Caenorhabditis elegans for the smell of pathogenic bacteria. Naive animals prefer smells of pathogens but animals trained with pathogens lose this attraction. We find that two different neural circuits subserve these preferences, with one required for the naive preference and the other specifically for the learned preference. Calcium imaging and behavioral analysis reveal that the naive preference reflects the direct transduction of the activity of olfactory sensory neurons into motor response, whereas the learned preference involves modulations to signal transduction to downstream neurons to alter motor response. Thus, two different neural circuits regulate a behavioral switch between naive and learned olfactory preferences.Organismic and Evolutionary BiologyPhysic
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Measurements of Natural Carbonate Rare Earth Elements in Femtogram Quantities by Inductive Coupled Plasma Sector Field Mass Spectrometry
A rapid and precise standard-bracketing method has been developed for measuring femtogram quantity rare earth element (REE) levels in natural carbonate samples by inductively coupled plasma sector field mass spectrometry that does not require chemical separation steps. A desolvation nebulization system was used to effectively reduce polyatomic interference and enhance sensitivity. REE/Ca ratios are calculated directly from the intensities of the ion beams of 46Ca, 139La, 140Ce, 141Pr, 146Nd, 147Sm, 153Eu, 160Gd, 159Tb, 163Dy, 165Ho, 166Er, 169Tm, 172Yb, and 175Lu using external matrix-matched synthetic standards to correct for instrumental ratio drifting and mass discrimination. A routine measurement time of 3 min is typical for one sample containing 20-40 ppm Ca. Replicate measurements made on natural coral and foraminiferal samples with REE/Ca ratios of 2-242 nmol/mol show that external precisions of 1.9-6.5% (2 RSD) can be achieved with only 10-1000 fg of REEs in 10-20 ÎŒg of carbonate. We show that different sources for monthly resolved coral ultratrace REE variability can be distinguished using this method. For natural slow growth-rate carbonate materials, such as sclerosponges, tufa, and speleothems, the high sample throughput, high precision, and high temporal resolution REE records that can be produced with this procedure have the potential to provide valuable time-series records to advance our understanding of paleoclimatic and paleoenvironmental dynamics on different time scales
Investigation of vancomycin- resistant Enterococci (VRE) colonization in a intensive care of medical center in southern Taiwan
Alanine aminotransferase to aspartate aminotransferase ratio and hepatitis B virus on metabolic syndrome: a community-based study
BackgroundThe serum aminotransferase elevation in metabolic syndrome (MetS) reflected hepatosteatosis, but there is a conflict with the coexistence of viral hepatitis, especially for the hepatitis B virus (HBV). Thus, this study aimed to investigate the relationship between the alanine aminotransferase (ALT)/aspartate aminotransferase (AST) ratio, MetS, and HBV infection in a rural Taiwanese population.MethodsWe conducted a cross-sectional analysis in southern Taiwan between March and December 2019. Multivariable logistic regression analyses adjusted for demographics, education, dietary behaviors, irregular exercise, substance use, and viral markers were performed to investigate the association between the ALT/AST ratio and MetS.ResultsAltogether, 2,416 participants (891 men and 1,525 women; mean age, 64.1 ± 14.9 years) were enrolled. Of the participants, 22.7% (n = 519) were seropositive for viral hepatitis. In the multivariable analysis, age [odds ratio (OR) 1.02, 95% CI 1.01â1.03, p < 0.001], ALT/AST ratio >1 (OR 2.63, 95% CI 2.15â3.21, p < 0.001), education (OR 0.96, 95% CI 0.94â0.98, p < 0.001), and HBV seropositivity (OR 0.70, 95% CI 0.52â0.95, p = 0.021) were associated with the risk of MetS. The area under the curve of the ALT/AST ratio was 0.62 (95% CI 0.60â0.64, p < 0.001), and the cutoff value was >0.852 for the Youden index.ConclusionAn ALT/AST ratio >1 could be a simple index for MetS prediction during community checkups. In contrast to age and betel nut chewing, HBV seropositivity and higher education might be inversely associated with MetS. Aggressive health promotion for MetS prevention has emerged as essential in participants without HBV and with lower education levels. Further large-scale, longitudinal studies are needed to unlink these correlations
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