Pharmacognostic analysis of Tudri Surkh (Cheiranthus cheiri)

Background: Tudri Surkh (Cheiranthus cheiri) is an important medicinal plant species used in Unani system of medicine. Despite having immense medicinal importance, little information is available on the standardization parameters of the species. For this reason, present work was carried out to establish pharmacognostical standards and generate comprehensive report on the quality control and standardization parameters of Tudri Surkh (Cheiranthus cheiri).Methods: Seeds of the plant were examined using microscopy and macroscopy, physicochemical parameters, extractive values, and fluorescence analysis.Results: The macroscopic, microscopy, and physicochemical parameters of seeds of Tudri Surkh (Cheiranthus cheiri) revealed various diagnostic characteristics in the species.Conclusions: This study provides complete pharmacognostic profile of Tudri Surkh (Cheiranthus cheiri) and hence will be useful for correct identification and authentication of the species for future studies

Combining multicolor FISH with fluorescence lifetime imaging for chromosomal identification and chromosomal sub structure investigation

Understanding the structure of chromatin in chromosomes during normal and diseased state of cells is still one of the key challenges in structural biology. Using DAPI staining alone together with Fluorescence lifetime imaging (FLIM), the environment of chromatin in chromosomes can be explored. Fluorescence lifetime can be used to probe the environment of a fluorophore such as energy transfer, pH and viscosity. Multicolor FISH (M-FISH) is a technique that allows individual chromosome identification, classification as well as assessment of the entire genome. Here we describe a combined approach using DAPI as a DNA environment sensor together with FLIM and M-FISH to understand the nanometer structure of all 46 chromosomes in the nucleus covering the entire human genome at the single cell level. Upon DAPI binding to DNA minor groove followed by fluorescence lifetime measurement and imaging by multiphoton excitation, structural differences in the chromosomes can be studied and observed. This manuscript provides a blow by blow account of the protocol required to perform M-FISH-FLIM of whole chromosomes

Assessing the Impact of Service Quality on Consumers Satisfaction: a Comparative Study of Commercial Vs Islamic Banks in Pakistan

This study aims to show the path that Islamic banking follows of Sharia' h while on the other hand commercial banks obeys commercial regulations and rules for the purpose of banking. The main objective of the Islamic banking is to offer interest free services and products which obeys the path of Sharia' h and Islamic banking system makes transaction on the foundation of profit and loss, while on the other hand commercial banks are business oriented banks. The sampling method used in this research was convenience sampling with a sample size of 200 which were the customers of the banks and the research design of the questionnaire used was exploratory The questionnaire was distributed in 4 banks 2 commercial and 2 Islamic banks respectively. Dependent variables are ‘customer expectation and perception' while on the other hand independent variable that are used this research are ‘reliability, assurance, tangibles, empathy and responsiveness. The results which are derived from this research shows a negative or less impact of two service quality dimensions on both banks which are Assurance and Empathy. The highest perceived service dimension is “Reliability,” that refers to the reliability of their products and promise to provide the best service quality to their customers. While the lowest perceived service was observed towards “Empathy,” the other two dimension with highest value are tangibility and responsiveness which means that customers' of both commercial and Islamic banks like tangible service and the quick responsiveness towards providing them the best service

Effect of fiber loading on mechanical and morphological properties of cocoa pod husk fibres reinforced thermoplastic polyurethane composites

In this study, cocoa (Theobroma cacao) pod husk (CPH) fiber reinforced thermoplastic polyurethane (TPU) was prepared by melt compounding method using Haake Polydrive R600 internal mixer. The composites were prepared with different fiber loading: 20%, 30% and 40% (by weight), with the optimum processing parameters: 190 °C, 11 min, and 40 rpm for temperature, time and speed, respectively. Five samples were cut from the composite sheet. Mean value was taken for each composite according to ASTM standards. Effect of fiber loading on mechanical (i.e. tensile, flexural properties and impact strength) and morphological properties was studied. TPU/CPH composites showed increase in tensile strength and modulus with increase in fiber loading, while tensile strain was decreasing with increase in fiber loading. The composite also showed increase in flexural strength and modulus with increase in fiber content. Impact strength was deteriorated with increase in fiber loading. Morphology observations using Scanning Electron Microscope (SEM) showed fiber/matrix good adhesion

Contribution of advanced fluorescence nano microscopy towards revealing mitotic chromosome structure

The organization of chromatin into higher-order structures and its condensation process represent one of the key challenges in structural biology. This is important for elucidating several disease states. To address this long-standing problem, development of advanced imaging methods has played an essential role in providing understanding into mitotic chromosome structure and compaction. Amongst these are two fast evolving fluorescence imaging technologies, specifically fluorescence lifetime imaging (FLIM) and super-resolution microscopy (SRM). FLIM in particular has been lacking in the application of chromosome research while SRM has been successfully applied although not widely. Both these techniques are capable of providing fluorescence imaging with nanometer information. SRM or nanoscopy is capable of generating images of DNA with less than 50 nm resolution while FLIM when coupled with energy transfer may provide less than 20 nm information. Here, we discuss the advantages and limitations of both methods followed by their contribution to mitotic chromosome studies. Furthermore, we highlight the future prospects of how advancements in new technologies can contribute in the field of chromosome science

N-acetyl-B-D-glucosaminidase and inflammatory response after cardiopulmonary bypass

OBJECTIVE: To determine the changes in activity of plasma N-acetyl-beta-D-glucosaminidase, a marker for inflammation as well as renal, pulmonary and cardiac damage and proinflammatory cytokines in patients undergoing coronary artery bypass grafting and find out the relationship between their plasma levels with clinical outcome of patients. STUDY DESIGN: Cross-sectional study. PLACE AND DURATION OF STUDY: The Aga Khan University, Karachi, from January to June 2003. PATIENTS AND METHODS: N-acetyl-beta-D-glucosaminidase (NAG) activity and concentrations of tumor necrosis factor-alpha of (TNFalpha), interleukin 6 (IL-6), interleukin 8 (IL8) and granulocyte-macrophage colony stimulating factor (GM-CSF) were monitored in plasma samples of 12 angina patients undergoing coronary artery bypass grafting (CABG), before, immediately after and 5 days post-surgical procedure. Serum glucose concentrations were also monitored in those patients. Patient\u27s clinical condition was monitored during this time period. RESULTS: No significant increase was observed in plasma NAG activity (a marker of inflammation) or in plasma levels of TNFalpha, IL-6, IL-8 and GM-CSF immediately after surgery, indicating that cardiopulmonary bypass itself does not produce any significant amount of inflammation immediately after CABG. However, 5 days post surgery, there was a significant increase in plasma NAG activity (p=0.001), TNFalpha (p=0.047) and GM-CSF (p=0.045). There was no relationship between plasma NAG activity and clinical outcome because various parameters of renal, cardiac and pulmonary functions, though slightly affected, remained within the normal limits. CONCLUSION: Increased levels of NAG and TNFalpha did not affect clinical outcome. However, data suggest that NAG can be a potential marker for inflammation and end organ damage following CABG. An increase in GM-CSF on day 5 following CABG indicates enhanced body\u27s defense mechanism against infection

Application of Electroporation Technique in Biofuel Processing

Biofuels production is mostly oriented with fermentation process, which requires fermentable sugar as nutrient for microbial growth. Lignocellulosic biomass (LCB) represents the most attractive, low-cost feedstock for biofuel production, it is now arousing great interest. The cellulose that is embedded in the lignin matrix has an insoluble, highly-crystalline structure, so it is difficult to hydrolyze into fermentable sugar or cell protein. On the other hand, microbial lipid has been studying as substitute of plant oils or animal fat to produce biodiesel. It is still a great challenge to extract maximum lipid from microbial cells (yeast, fungi, algae) investing minimum energy. Electroporation (EP) of LCB results a significant increase in cell conductivity and permeability caused due to the application of an external electric field. EP is required to alter the size and structure of the biomass, to reduce the cellulose crystallinity, and increase their porosity as well as chemical composition, so that the hydrolysis of the carbohydrate fraction to monomeric sugars can be achieved rapidly and with greater yields. Furthermore, EP has a great potential to disrupt the microbial cell walls within few seconds to bring out the intracellular materials (lipid) to the solution. Therefore, this study aims to describe the challenges and prospect of application of EP technique in biofuels processing

Using Tumor-Like Spheroids to Study the Effect of Anti-Cancer Drugs <em>in vitro</em>

Cell culture techniques have evolved in the last decades and allow now testing anti-cancer drugs using tumor-like spheroids. We describe here issues and trouble-shooting solutions when generating spheroids from three human melanoma cell lines (A375, WM115 and WM266). A375 cells generated irregular shape spheroids that were difficult to study due to their fragility. Spheroids generated from all cell lines initially reduced their diameter and increased compacity before increasing in size overtime. Cells present at the periphery of the spheroids showed higher metabolic activity than cells present in the core of the spheroids. When grown as spheroids, a smaller fraction of the A375 and WM115 cells was sensitive to the chemotherapeutic agent temozolomide as compared to cells grown on flat surface. However, this difference was not observed with WM266 cells. Although the presence of spheroids resulted in a smaller fraction of WM155 cells sensitive to the anti-cancer agent vemurafenib, the opposite was observed with A375 cells. Among the cells, WM266 cells were the most resistant to vemurafenib. In conclusion, our study suggests that cell lines behave differently in terms of spheroid formation, and that the effect of the 3D cellular architecture on drug effect is cell type and drug dependent

Data-driven generation of 4D velocity profiles in the aneurysmal ascending aorta

Numerical simulations of blood flow are a valuable tool to investigate the pathophysiology of ascending thoracic aortic aneurysms (ATAA). To accurately reproduce hemodynamics, computational fluid dynamics (CFD) models must employ realistic inflow boundary conditions (BCs). However, the limited availability of in vivo velocity measurements still makes researchers resort to idealized BCs. In this study we generated and thoroughly characterized a large dataset of synthetic 4D aortic velocity profiles suitable to be used as BCs for CFD simulations. 4D flow MRI scans of 30 subjects with ATAA were processed to extract cross-sectional planes along the ascending aorta, ensuring spatial alignment among all planes and interpolating all velocity fields to a reference configuration. Velocity profiles of the clinical cohort were extensively characterized by computing flow morphology descriptors of both spatial and temporal features. By exploiting principal component analysis (PCA), a statistical shape model (SSM) of 4D aortic velocity profiles was built and a dataset of 437 synthetic cases with realistic properties was generated. Comparison between clinical and synthetic datasets showed that the synthetic data presented similar characteristics as the clinical population in terms of key morphological parameters. The average velocity profile qualitatively resembled a parabolic-shaped profile, but was quantitatively characterized by more complex flow patterns which an idealized profile would not replicate. Statistically significant correlations were found between PCA principal modes of variation and flow descriptors. We built a data-driven generative model of 4D aortic velocity profiles, suitable to be used in computational studies of blood flow. The proposed software system also allows to map any of the generated velocity profiles to the inlet plane of any virtual subject given its coordinate set.Comment: 21 pages, 5 figures, 2 tables To be submitted to "Computer methods and programs in biomedicine" Scripts: https://github.com/saitta-s/flow4D Synthetic velocity profiles: //doi.org/10.5281/zenodo.725198

The effects of changing land use and flood hazard on poverty in coastal Bangladesh

The construction of polders in the coastal region of Bangladesh has significantly modified the patterns of flooding, as well as leading to significant land use/land cover (hereinafter, LULC) changes. The impact of LULC change and flooding on poverty is complex and poorly understood. This study presents a spatiotemporal appraisal of poverty in relation to LULC change and pluvial flood risk in the south western embanked area of Bangladesh. A combination of logistic regression (LR), cellular automata (CA), and Markov Chain models were utilised to predict future LULC based on historical data. Flood risk assessment was performed at present and for future LULC scenarios. A spatial regression model was developed, incorporating multiple parameters to estimate the wealth index (WI) for present-day and future scenarios. In the study area, agricultural lands reduced from 34 % in 2005 to 8% in 2010, while aquaculture land cover increased from 17 % to 39 % during the same time. The rate of LULC change was relatively low between 2010 and 2019. Based on the recent trend, LULC was predicted for the year 2030. Flood risk was positively correlated with LULC and the expected annual damage (EAD) was estimated at $903 million in 2005, which is likely to increase to$2096 million by 2030, considering changes in LULC scenarios. The analysis further showed that the EAD and LULC change were negatively associated with the WI. Despite consistent national GDP growth in Bangladesh in recent years, the rate of increase of WI is likely to be low in the future because flood risk and patterns of LULC change have a negative effect on WI