Utilization of Honey Apis dorsata as Antiosteoporosis on Requirements of Bone Calcium Ash Density on Ovariohysterectomized White Rat (Ratus norvegicus)

The purpose of this study was to determine the effect of honey bee Apis dorsata as anti-osteoporosis in calcium ash density (CAD) of bone in osteoporotic-induced rats. The target of this study was to know bone calcium levels after being given honey bees Apis dorsata. In this study, 35 female white rats (Ratus norvegicus) mature was used with weight 200gr. Divided into 5 groups, 2 control groups and 3 treatment groups. The negative control group (SH) was not induced by osteoporosis and was given the only aquadest of 1.5 ml/day. Whereas the positive control group was induced by osteoporosis (OH)  and was given only aquadest 1.5 ml/day. T1, T2 and T3 treatment groups were given bee honey with various doses including 1g / kg ad 1.5 ml aquadest, 2g / kg BB ad 1.5 ml aquadest and 3g / kg BB ad 1.5 ml aquadest. Then after 12 weeks, white rats were sacrificed for lumbar vertebrae. Furthermore, the sixth lumbar spine os vertebrae will be examined for calcium bone ash content. The data were obtained was analyzed using statistical analysis of variance (ANOVA) for the results of the calcium ash content data Keywords: Honey; Osteoporosis; CA

MANFAAT PEMBERIAN MADU LEBAH (Apis dorsata) UNTUK MENCEGAH OSTEOPOROSIS BERDASARKAN PERLUASAN LAKUNA TULANG VERTEBRAE LUMBALIS TIKUS PUTIH (Rattus norvegicus) SETELAH OVARIOHISTEREKTOMI

The aim of this research was to determine the potential of honey bee (Apis dorsata) in preventing osteoporosis based on the expansion of lacuna vertebrae of white rat (Rattus norvegicus) after ovariohysterectomy. The samples were 20 female rats adapted for 7 days in cluster cage and divided randomly into 5 groups (SO, OH, M1, M2, M3). SO was a sham operated groups which non ovariohysterectomized rats that received aquadest. OH is an ovariohysterectomized groups which is the rats were ovariohysterectomized and received aquadest. M1 (1 g/kgBW), M2 (2 g/kgBW) and M3 (4 g/kgBW) were ovarohysterectomized rats and received honey solutions of Apis dorsata. Honey be diluted in 1 ml aquadest and given orally for 84 days. On the 85th day rats were euthanized and vertebrae lumbar was taken with necroption technique. Lacuna area and scoring percentage of expansion were measured using optilab microscope. The result show there was significant difference (p<0.05) on Lakuna area and scoring percentage between OH group and all of the groups. The result of the highest scoring percentage are on M2 group and the lowest are on OH group. The result of the lowest lakuna area are on M2 group and the highest are on OH group

Analisis Scanning Electron Microscope (SEM) Mikroarsitektur Daerah Metafisis Os Femur Tikus Putih (Rattus novergicus) Ovariohisterektomi dengan Pemberian Madu Lebah (Apis dorsata) Hutan Sumbawa

The aims of this study to determine the effect of honey bees (Apis dorsata) to prevent the decrease of femur density in  ovariohysterctomized rats as animal model of osteoporosis by Scanning Electron Microscope (SEM). This study used 20 adult female white rats (Rattus norvegicus) with body weight 300 gr. Divided into 5 groups, negative control group (P0-), not in ovariohysterectomy,  only given aquadest 1.5 ml/day ; Positive control group (P0 +), in ovariohysterectomy,  only given aquadest 1.5 ml/day; Treatment group 1 (P1), in ovariohysterectomy and given honey 1 gram / kg BB + 1.5 ml aquadest; Treatment group 2 (P2), in ovariohysterectomy and given honey 2 grams / kg BB + 1.5 ml aquadest; Treatment group 3 (P3), in ovariohysterectomy and given honey 4 grams / kg BB + 1.5 ml aquadest. After 12 weeks, all rats were sacrified for removal of the femur and performed by SEM. Analysis of SEM showed that mice administered honey with a dose of 4 grams / kg BW (P3) showed bone density approaching negative control treatment (P0-), ie, ovariohysterectomized rats. P3 showed the most less bone porosity than other treatment groups. The largest and most uniformly distributed porosity was shown by the positive control group (P0 +). The best results with the smallest quantity and size of the porosity of femur is in P3 group. In conclusion, honey (Apis dorsata) can prevent the decrease in bone density of the femur by Scanning Electron Microscope (SEM)