Sonication assisted Agrobacterium-mediated transformation of chalcone synthase (CHS) gene to Spring Dendrobium cultivar ‘Sanya’

In this study, a protocol was developed to obtain stable lines of the Spring Dendrobium cultivar ‘Sanya’ via sonication assisted Agrobacterium-mediated transformation (SAAT) of protocorm-like bodies (PLBs). Agrobacterium tumefaciens strain LBA4404 was used with the binary vector AG205 containing the chalcone synthase (CHS) gene for flower color change and the neomycin phosphotransferase II gene (NPTIIgene) as a selectable marker for kanamycin resistance. PLBs were treated for 10 min using ultrasound (40 KHz) and subsequently immersed in the Agrobacterium suspension for 60 min. The addition of 100 M acetosyringone (AS) during the pre-culture and co-culture achieved the highest efficiency of gene transformation (0.5%). Following co-cultivation, PLBs were cultured on selective medium containing 200 mgL-1 kanamycin and 250 mgL-1 cefotaxime. Proliferating PLBs were repeatedly selected for kanamycin resistance and stable transformed lines were generated. Incorporation and expression of transgenes were confirmed by PCR analysis, southern blot analysis and reverse transcription-quantitative real-time PCR (RT-PCR). A total of five southern blot positive lines were obtained from co-cultivated PLBs.Key words: Spring Dendrobium cultivar ‘Sanya’, chalcone synthase (CHS) gene, transformation, sonication assisted Agrobacterium-mediated transformation (SAAT), protocorm-like bodies (PLBs)

Detection of homozygosity in near isogenic Lines of non-susceptible to Zhenjiang strain of densonucleosis virus in silkworm

The near isogenic lines (NILs) of non-susceptible to DNV-Z were bred through backcrossing successively using L10 as donor parent and Js as recurrent parent. The homozygosity of the NILs was detected using the SSR markers from the SSR linkage map. The results showed that the ratio of the linkage groups from the recurrent parent in the NILs increased rapidly along with the increase of the backcrossing generations. After 6 generations of backcrossing, it was selfed and was fed with DNV-Z. The linkage groups of 9 of the 5 BC6 pairs, whose 1/4 offspring were non-susceptible to DNV-Z, were all replaced by the recurrent parent except the linkage group that held nsd-Z, and only part of one linkage group from the other one individual had not been replaced. According to the markers linked to nsd-Z, Fl0316 had not been replaced in all of the 10 individuals, but crossover happened in 2 individuals between Fl0568 and nsd-Z

Structure and stability of quasi-two-dimensional boson-fermion mixtures with vortex-antivortex superposed states

We investigate the equilibrium properties of a quasi-two-dimensional degenerate boson-fermion mixture (DBFM) with a bosonic vortex-antivortex superposed state (VAVSS) using a quantum-hydrodynamic model. We show that, depending on the choice of parameters, the DBFM with a VAVSS can exhibit rich phase structures. For repulsive boson-fermion (BF) interaction, the Bose-Einstein condensate (BEC) may constitute a petal-shaped "core" inside the honeycomb-like fermionic component, or a ring-shaped joint "shell" around the onion-like fermionic cloud, or multiple segregated "islands" embedded in the disc-shaped Fermi gas. For attractive BF interaction just below the threshold for collapse, an almost complete mixing between the bosonic and fermionic components is formed, where the fermionic component tends to mimic a bosonic VAVSS. The influence of an anharmonic trap on the density distributions of the DBFM with a bosonic VAVSS is discussed. In addition, a stability region for different cases of DBFM (without vortex, with a bosonic vortex, and with a bosonic VAVSS) with specific parameters is given.Comment: 8 pages,5 figure

Advances in targeted therapy for acute myeloid leukemia.

Acute myeloid leukemia (AML) is a clonal malignancy characterized by genetic heterogeneity due to recurrent gene mutations. Treatment with cytotoxic chemotherapy has been the standard of care for more than half of a century. Although much progress has been made toward improving treatment related mortality rate in the past few decades, long term overall survival has stagnated. Exciting developments of gene mutation-targeted therapeutic agents are now changing the landscape in AML treatment. New agents offer more clinical options for patients and also confer a more promising outcome. Since Midostaurin, a FLT3 inhibitor, was first approved by US FDA in 2017 as the first gene mutation-targeted therapeutic agent, an array of new gene mutation-targeted agents are now available for AML treatment. In this review, we will summarize the recent advances in gene mutation-targeted therapies for patients with AML

Meta-analysis of prophylactic corticosteroid use in post-ERCP pancreatitis

<p>Abstract</p> <p>Background</p> <p>Acute pancreatitis is a common complication of endoscopic retrograde cholangiopancreatography and benefit of pharmacological treatment is unclear. Although prophylactic use of corticosteroid for reduction of pancreatic injury after ERCP has been evaluated, discrepancy about beneficial effect of corticosteroid on pancreatic injury still exists. The aim of current study is to evaluate effectiveness and safety of corticosteroid in prophylaxis of post-endoscopic retrograde cholangiopancreatography pancreatitis (PEP).</p> <p>Methods</p> <p>We employed the method recommended by the Cochrane Collaboration to perform a meta-analysis of seven randomized controlled trials (RCTs) of corticosteroid in prevention of post-ERCP pancreatitis (PEP) around the world.</p> <p>Results</p> <p>Most of the seven RCTs were of high quality. When the RCTs were analyzed, odds ratios (OR) for corticosteroid were 1.13 [95% CI (0.89~1.44), p = 0.32] for PEP, 1.61 [95% CI (0.74~3.52), p = 0.23] for severe PEP, 0.92 [95% CI (0.57~1.48), p = 0.73] for post-ERCP hyperamylasemia respectively. The results indicated that there were no beneficial effects of corticosteroid on acute pancreatitis and hyperamylasemia. No evidence of publication bias was found.</p> <p>Conclusion</p> <p>Corticosteroids cannot prevent pancreatic injury after ERCP. Therefore, their use in the prophylaxis of PEP is not recommended.</p

Upregulation of miR-196b Confers a Poor Prognosis in Glioblastoma Patients via Inducing a Proliferative Phenotype

PURPOSE: To explore the expression pattern, prognostic value and functional role of miR-196b in glioblastoma (GBM) patients using large cohorts. EXPERIMENTAL DESIGN: MiR-196b expression was measured using the Human v2.0 miRNA Expression BeadChip (Illumina) in 198 frozen glioma tissues. The expression levels of miR-196b were also validated in an independent cohort containing 128 formalin-fixed paraffin-embedded (FFPE) glioma samples using qRT-PCR. The presence of other molecular prognostic indicators was assessed centrally in the glioma samples. Whole genome gene profiling was performed to investigate the underlying biological behavior. MiR-196b functional analyses were performed in U87 and U251 cell lines. RESULTS: The expression levels of miR-196b were inversely correlated with overall survival in GBM patients. Gene set enrichment analysis (GSEA) showed that the gene sets relating to cell cycle were significantly enriched in the cases with miR-196b overexpression. Functional analyses in U87 and U251 cells revealed that miR-196b was involved in cell proliferation. CONCLUSIONS: MiR-196b is overexpressed and confers a poor prognosis via promoting cellular proliferation in GBM patients

Atg7-Mediated Autophagy Is Involved in the Neural Crest Cell Generation in Chick Embryo

Autophagy plays a very important role in numerous physiological and pathological events. However, it still remains unclear whether Atg7-induced autophagy is involved in the regulation of neural crest cell production. In this study, we found the co-location of Atg7 and Pax7+ neural crest cells in early chick embryo development. Upregulation of Atg7 with unilateral transfection of full-length Atg7 increased Pax7+ and HNK-1+ cephalic and trunk neural crest cell numbers compared to either Control-GFP transfection or opposite neural tubes, suggesting that Atg7 over-expression in neural tubes could enhance the production of neural crest cells. BMP4 in situ hybridization and p-Smad1/5/8 immunofluorescent staining demonstrated that upregulation of Atg7 in neural tubes suppressed the BMP4/Smad signaling, which is considered to promote the delamination of neural crest cells. Interestingly, upregulation of Atg7 in neural tubes could significantly accelerate cell progression into the S phase, implying that Atg7 modulates cell cycle progression. However, β-catenin expression was not significantly altered. Finally, we demonstrated that upregulation of the Atg7 gene could activate autophagy as did Atg8. We have also observed that similar phenotypes, such as more HNK-1+ neural crest cells in the unilateral Atg8 transfection side of neural tubes, and the transfection with full-length Atg8-GFP certainly promote the numbers of BrdU+ neural crest cells in comparison to the GFP control. Taken together, we reveal that Atg7-induced autophagy is involved in regulating the production of neural crest cells in early chick embryos through the modification of the cell cycle