THE PREVALENCE OF UNHEALTHY SNACKING BEHAVIOUR AND ITS ASSOCIATION WITH INDIVIDUAL AND ENVIRONMENTAL FACTORS AMONG COLLEGE STUDENTS IN KUCHING, SARAWAK

Unhealthy snacking behaviour among young people is a growing public health concern because of its negative consequences on health. This study was to determine the prevalence of unhealthy snacking behaviour and its determinants among college students in Kuching. In this cross-sectional study, 12 colleges in Kuching were stratified into private and government colleges. Three private and three government colleges were selected through a stratified random sampling method. A total of 422 respondents (male 49.5%, female 50.5%) were recruited in this study. A self-administered questionnaire was used to gather data regarding unhealthy snacking behaviour and its determinants (gender, food preferences, type of school and courses, taste-and-sensory perception, nutritional knowledge, perceived self-efficacy, and cost/price sensitivity, perceived parental control, easy accessibility, and media advertisements influence) among respondents. The survey was conducted online via e-mail/Whatsapp. Multiple binary logistic regression analysis was used to analyse the data. A quarter of the respondents (24.6%) practised unhealthy snacking behaviour. More than half of the respondents (58.3%) consumed unhealthy snacks one to three times per week. A minority of the respondents (17.1%) claimed that they did not consume any unhealthy snacks for the past month. The final model explained 15.7% of the total variance. Taste-and-sensory perception (Adjusted OR = 2.20, 95%CI [1.55, 3.13], p < 0.001) and nutritional knowledge (Adjusted OR = 1.48, 95%CI [1.12, 1.97), p < 0.05) were positively associated with unhealthy snacking behaviour among respondents. The present study suggested the stakeholders concentrate on nutritional knowledge and taste-and-sensory perception among college students in future intervention developmen

A new prognostic histopathologic classification of nasopharyngeal carcinoma

Background: The current World Health Organization (WHO) classification of nasopharyngeal carcinoma (NPC) conveys little prognostic information. This study aimed to propose an NPC histopathologic classification that can potentially be used to predict prognosis and treatment response. Methods: We initially developed a histopathologic classification based on the morphologic traits and cell differentiation of tumors of 2716 NPC patients who were identified at Sun Yat-sen University Cancer Center (SYSUCC) (training cohort). Then, the proposed classification was applied to 1702 patients (retrospective validation cohort) from hospitals outside SYSUCC and 1613 patients (prospective validation cohort) from SYSUCC. The efficacy of radiochemotherapy and radiotherapy modalities was compared between the proposed subtypes. We used Cox proportional hazards models to estimate hazard ratios (HRs) with 95% confidence intervals (CI) for overall survival (OS). Results: The 5-year OS rates for all NPC patients who were diagnosed with epithelial carcinoma (EC; 3708 patients), mixed sarcomatoid-epithelial carcinoma (MSEC; 1247 patients), sarcomatoid carcinoma (SC; 823 patients), and squamous cell carcinoma (SCC; 253 patients) were 79.4%, 70.5%, 59.6%, and 42.6%, respectively (P < 0.001). In multivariate models, patients with MSEC had a shorter OS than patients with EC (HR = 1.44, 95% CI = 1.27–1.62), SC (HR = 2.00, 95% CI = 1.76–2.28), or SCC (HR = 4.23, 95% CI = 3.34–5.38). Radiochemotherapy significantly improved survival compared with radiotherapy alone for patients with EC (HR = 0.67, 95% CI = 0.56–0.80), MSEC (HR = 0.58, 95% CI = 0.49–0.75), and possibly for those with SCC (HR = 0.63; 95% CI = 0.40–0.98), but not for patients with SC (HR = 0.97, 95% CI = 0.74–1.28). Conclusions: The proposed classification offers more information for the prediction of NPC prognosis compared with the WHO classification and might be a valuable tool to guide treatment decisions for subtypes that are associated with a poor prognosis

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals &lt;1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

The prevalence of unhealthy snacking behaviour and its association with individual and environmental factors among college students in Kuching, Sarawak

Introduction: Unhealthy snacking behaviour among young people is a growing public health concern because of its negative consequences on health. The present study was to determine the prevalence of unhealthy snacking behaviour and its determinants among college students in Kuching. Methodology: In this crosssectional study, 12 colleges in Kuching were stratified into private and government colleges. Three private and three government colleges were selected through a stratified random sampling method. A total of 422 respondents (male 49.5%, female 50.5%) were recruited in this study. A self-administered questionnaire was used to gather data regarding unhealthy snacking behaviour and its determinants (gender, food preferences, type of school and courses, taste-and-sensory perception, nutritional knowledge, perceived self-efficacy, and cost/price sensitivity, perceived parental control, easy accessibility, and media advertisements influence) among respondents. The survey was conducted online via e-mail and Whatsapp. Multiple binary logistic regression analysis was used to analyse the data. Result: Nearly a quarter of the respondents (24.6%) practised unhealthy snacking behaviour. More than half of the respondents (58.3%) consumed unhealthy snacks one to three times per week. Only a minority of the respondents (17.1%) claimed that they did not consume any unhealthy snacks for the past month. The final model explained 15.7% of the total variance. Taste-and-sensory perception (Adjusted OR = 2.20, 95% Cl = 1.55 - 3.13, p < 0.001) and nutritional knowledge (Adjusted OR = 1.48, 95% Cl =1.12 - 1.97, p < 0.05) were positively associated with unhealthy snacking behaviour among respondents. Conclusion: The present study suggested the stakeholders for future intervention development by concentrate on nutritional knowledge and tasteand-sensory perception among college students. Moreover, governments may also intervene in unhealthy snack companies by refining certain food policies and strengthen the enforcement of food laws

Chemistry and biochemistry of Terpenoids from Curcumaand related species

Several curcuminoids have been identified from rhizome of the common spice Curcuma longa (Zingaberaceae) and related plant species. Curcuminoids are known to display several pharmacological properties summed up in numerous papers and reviews. In addition to curcuminoids, more than 250 mono-, sesqui- di-, and triterpenoids have been identified from curcuma species. These lipophilic compounds have better absorption than curcuminoids and also exhibit a wide spectrum of pharmacological properties. Little attention has been paid to these lipophilic compounds, which may be as physiologically active, if not more, as curcuminoids. This review focuses on Curcuma terpenoids and their physiological properties