900 research outputs found
Properties of Central Caustics in Planetary Microlensing
To maximize the number of planet detections, current microlensing follow-up
observations are focusing on high-magnification events which have a higher
chance of being perturbed by central caustics. In this paper, we investigate
the properties of central caustics and the perturbations induced by them. We
derive analytic expressions of the location, size, and shape of the central
caustic as a function of the star-planet separation, , and the planet/star
mass ratio, , under the planetary perturbative approximation and compare the
results with those based on numerical computations. While it has been known
that the size of the planetary caustic is \propto \sqrt{q}, we find from this
work that the dependence of the size of the central caustic on is linear,
i.e., \propto q, implying that the central caustic shrinks much more rapidly
with the decrease of compared to the planetary caustic. The central-caustic
size depends also on the star-planet separation. If the size of the caustic is
defined as the separation between the two cusps on the star-planet axis
(horizontal width), we find that the dependence of the central-caustic size on
the separation is \propto (s+1/s). While the size of the central caustic
depends both on and q, its shape defined as the vertical/horizontal width
ratio, R_c, is solely dependent on the planetary separation and we derive an
analytic relation between R_c and s. Due to the smaller size of the central
caustic combined with much more rapid decrease of its size with the decrease of
q, the effect of finite source size on the perturbation induced by the central
caustic is much more severe than the effect on the perturbation induced by the
planetary caustic. Abridged.Comment: 5 pages, 4 figures, ApJ accepte
Mobile resistome of human gut and pathogen drives anthropogenic bloom of antibiotic resistance
BACKGROUND:The impact of human activities on the environmental resistome has been documented in many studies, but there remains the controversial question of whether the increased antibiotic resistance observed in anthropogenically impacted environments is just a result of contamination by resistant fecal microbes or is mediated by indigenous environmental organisms. Here, to determine exactly how anthropogenic influences shape the environmental resistome, we resolved the microbiome, resistome, and mobilome of the planktonic microbial communities along a single river, the Han, which spans a gradient of human activities. RESULTS:The bloom of antibiotic resistance genes (ARGs) was evident in the downstream regions and distinct successional dynamics of the river resistome occurred across the spatial continuum. We identified a number of widespread ARG sequences shared between the river, human gut, and pathogenic bacteria. These human-related ARGs were largely associated with mobile genetic elements rather than particular gut taxa and mainly responsible for anthropogenically driven bloom of the downstream river resistome. Furthermore, both sequence- and phenotype-based analyses revealed environmental relatives of clinically important proteobacteria as major carriers of these ARGs. CONCLUSIONS:Our results demonstrate a more nuanced view of the impact of anthropogenic activities on the river resistome: fecal contamination is present and allows the transmission of ARGs to the environmental resistome, but these mobile genes rather than resistant fecal bacteria proliferate in environmental relatives of their original hosts. Video abstract
IGFBP-3 Inhibits Cytokine-Induced Insulin Resistance and Early Manifestations of Atherosclerosis
Metabolic syndrome is associated with visceral obesity, insulin resistance and an increased risk of cardiovascular diseases. Visceral fat tissue primarily consists of adipocytes that secrete cytokines leading to a state of systemic inflammation in obese conditions. One of the IGF-independent functions of IGFBP-3 is its role as an anti-inflammatory molecule. Our study in obese adolescents show a decrease in total IGFBP-3 levels and increase in proteolyzed IGFBP-3 in circulation when compared to their normal counterparts and establishes a positive correlation between IGFBP-3 proteolysis and adiposity parameters as well as insulin resistance. In human adipocytes, we show that IGFBP-3 inhibits TNF-α-induced NF-ÎșB activity in an IGF-independent manner, thereby restoring the deregulated insulin signaling and negating TNF-α-induced inhibition of glucose uptake. IGFBP-3 further inhibits TNF-α, CRP and high glucose-induced NF-ÎșB activity in human aortic endothelial cells (HAECs) and subsequently suppresses monocyte adhesion to HAEC through the IGFBP-3 receptor. In conclusion, these findings suggest that reduced levels of IGFBP-3 in circulation and reduced expression of IGFBP-3 in macrophages in obesity may result in suppression of its anti-inflammatory functions and therefore IGFBP-3 may present itself as a therapeutic for obesity-induced insulin resistance and for events occurring in the early stages of atherosclerosis
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Effect of Electrode Composition and Microstructure on Impendancemetric nitric oxide sensors based on YSZ Electrolyte
The role of metal (Au, Pt, and Ag) electrodes in yttria-stabilized zirconia (YSZ) electrolyte-based impedancemetric nitric oxide (NO) sensors is investigated using impedance spectroscopy and equivalent circuit analysis. Focus on the metal/porous YSZ interface is based on previous studies using a symmetric cell (metal/YSZporous/YSZdense/YSZporous/metal) and attempts to further elucidate the important processes responsible for sensing. The current test cell consists of a rectangular slab of porous YSZ with two metal-wire loop electrodes (metal/YSZporous/metal), both exposed to the same atmosphere. Of the electrode materials, only Au was sensitive to changes in NO concentration. The impedance behavior of porous Au electrodes in a slightly different configuration was compared with dense Au electrodes and was also insensitive to NO. Although the exact mechanism is not determined, the composition and microstructure of the metal electrode seem to alter the rate-limiting step of the interfering O2 reaction. Impedance behavior of the O2 reaction that is limited by processes occurring away from the triple-phase boundary may be crucial for impedancemetric NO sensing
Raman Scattered He II Line in the Planetary Nebula M2-9 and the Symbiotic Stars RR Telescopii and He 2-106
In this Letter, we report the detection of an emission feature around 6545
\AA in the spectra of the bipolar planetary nebula M2-9 and the symbiotic stars
RR Telescopii and He 2-106 and propose to identify it as the He II Raman
scattered feature. This feature was predicted by Nussbaumer, Schmid & Vogel
(1989), who suggested that it is formed through Raman scattering by atomic
hydrogen of He II photons with slightly shorter wavelength than
that of Ly. The scattering cross section for
this process implies the existence of a neutral hydrogen component with a
column density around the He II emission
regions in these objects, which are believed to be associated with the mass
loss process in the late stage of stellar evolution. Brief discussions on the
astrophysical implications of Raman scattering in these objects are presented.Comment: 8 pages, 2 figures, accepted for publication in the Astrophysical
Journal Letter
X-ray Astronomy in the Laboratory with a Miniature Compact Object Produced by Laser-Driven Implosion
Laboratory spectroscopy of non-thermal equilibrium plasmas photoionized by
intense radiation is a key to understanding compact objects, such as black
holes, based on astronomical observations. This paper describes an experiment
to study photoionizing plasmas in laboratory under well-defined and genuine
conditions. Photoionized plasma is here generated using a 0.5-keV Planckian
x-ray source created by means of a laser-driven implosion. The measured x-ray
spectrum from the photoionized silicon plasma resembles those observed from the
binary stars Cygnus X-3 and Vela X-1 with the Chandra x-ray satellite. This
demonstrates that an extreme radiation field was produced in the laboratory,
however, the theoretical interpretation of the laboratory spectrum
significantly contradicts the generally accepted explanations in x-ray
astronomy. This model experiment offers a novel test bed for validation and
verification of computational codes used in x-ray astronomy.Comment: 5 pages, 4 figures are included. This is the original submitted
version of the manuscript to be published in Nature Physic
GALEX Ultraviolet Photometry of Globular Clusters in M31
We present ultraviolet photometry for globular clusters (GCs) in M31 from 15
square deg of imaging using the Galaxy Evolution Explorer (GALEX). We detect
200 and 94 GCs with certainty in the near-ultraviolet (NUV; 1750 - 2750
Angstroms) and far-ultraviolet (FUV; 1350 - 1750 Angstroms) bandpasses,
respectively. Our rate of detection is about 50% in the NUV and 23% in the FUV,
to an approximate limiting V magnitude of 19. Out of six clusters with
[Fe/H]>-1 seen in the NUV, none is detected in the FUV bandpass. Furthermore,
we find no candidate metal-rich clusters with significant FUV flux, because of
the contribution of blue horizontal-branch (HB) stars, such as NGC 6388 and NGC
6441, which are metal-rich Galactic GCs with hot HB stars. We show that our
GALEX photometry follows the general color trends established in previous UV
studies of GCs in M31 and the Galaxy. Comparing our data with Galactic GCs in
the UV and with population synthesis models, we suggest that the age range of
M31 and Galactic halo GCs are similar.Comment: This paper will be published as part of the Galaxy Evolution Explorer
(GALEX) Astrophysical Journal Letters Special Issue. Links to the full set of
papers will be available at http://www.galex.caltech.edu/PUBLICATIONS/ after
November 22, 200
The nuclear immune receptor RPS4 is required for RRS1SLH1-dependent constitutive defense activation in Arabidopsis thaliana
Plant nucleotide-binding leucine-rich repeat (NB-LRR) disease resistance (R) proteins recognize specific ââavirulentââ pathogen effectors and activate immune responses. NB-LRR proteins structurally and functionally resemble mammalian Nod-like receptors (NLRs). How NB-LRR and NLR proteins activate defense is poorly understood. The divergently transcribed Arabidopsis R genes, RPS4 (resistance to Pseudomonas syringae 4) and RRS1 (resistance to Ralstonia solanacearum 1), function together to confer recognition of Pseudomonas AvrRps4 and Ralstonia PopP2. RRS1 is the only known recessive NBLRR R gene and encodes a WRKY DNA binding domain, prompting suggestions that it acts downstream of RPS4 for transcriptional activation of defense genes. We define here the early RRS1-dependent transcriptional changes upon delivery of PopP2 via Pseudomonas type III secretion. The Arabidopsis slh1 (sensitive to low humidity 1) mutant encodes an RRS1 allele (RRS1SLH1) with a single amino acid (leucine) insertion in the WRKY DNA-binding domain. Its poor growth due to constitutive defense activation is rescued at higher temperature. Transcription profiling data indicate that RRS1SLH1-mediated defense activation overlaps substantially with AvrRps4- and PopP2-regulated responses. To better understand the genetic basis of RPS4/RRS1-dependent immunity, we performed a genetic screen to identify suppressor of slh1 immunity (sushi) mutants. We show that many sushi mutants carry mutations in RPS4, suggesting that RPS4 acts downstream or in a complex with RRS1. Interestingly, several mutations were identified in a domain C-terminal to the RPS4 LRR domain. Using an Agrobacterium-mediated transient assay system, we demonstrate that the P-loop motif of RPS4 but not of RRS1SLH1 is required for RRS1SLH1 function. We also recapitulate the dominant suppression of RRS1SLH1 defense activation by wild type RRS1 and show this suppression requires an intact RRS1 P-loop. These analyses of RRS1SLH1 shed new light on mechanisms by which NB-LRR protein pairs activate defense signaling, or are held inactive in the absence of a pathogen effector
Xylitol production is increased by expression of codon-optimized Neurospora crassa xylose reductase gene in Candida tropicalis
Xylose reductase (XR) is the first enzyme in d-xylose metabolism, catalyzing the reduction of d-xylose to xylitol. Formation of XR in the yeast Candida tropicalis is significantly repressed in cells grown on medium that contains glucose as carbon and energy source, because of the repressive effect of glucose. This is one reason why glucose is not a suitable co-substrate for cell growth in industrial xylitol production. XR from the ascomycete Neurospora crassa (NcXR) has high catalytic efficiency; however, NcXR is not expressed in C. tropicalis because of difference in codon usage between the two species. In this study, NcXR codons were changed to those preferred in C. tropicalis. This codon-optimized NcXR gene (termed NXRG) was placed under control of a constitutive glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter derived from C. tropicalis, and integrated into the genome of xylitol dehydrogenase gene (XYL2)-disrupted C. tropicalis. High expression level of NXRG was confirmed by determining XR activity in cells grown on glucose medium. The resulting recombinant strain, LNG2, showed high XR activity (2.86 U (mg of protein)â1), whereas parent strain BSXDH-3 showed no activity. In xylitol fermentation using glucose as a co-substrate with xylose, LNG2 showed xylitol production rate 1.44 g Lâ1 hâ1 and xylitol yield of 96% at 44 h, which were 73 and 62%, respectively, higher than corresponding values for BSXDH-3 (rate 0.83 g Lâ1 hâ1; yield 59%)
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