Evaluation of the in vivo role of vaccinia virus complement control protein (VCP) following renal ischemia

Includes bibliographical references (leaves 133-147)In transplantation, vascularized organs often suffer the consequences of ischemic damage as well as reperfusion injury following the reestablishment of blood flow. The induced ischemialreperfusion (I/R) damage is usually associated with the accumulation of injurious complement components. The vaccinia virus complement control protein (VCP) has the ability to simultaneously inhibit the classical and the alternative complement pathways by binding to the early components C3b and C4b. The complement component C3 is known to be the central route to all of the known complement activation pathways. As a result, it is involved in a number of complement-mediated ailments including renal ischemia/reperfusion injury. The objectives of this study were to initially evaluate the in vitro roles of the natural VCP and the humanized recombinant VCP (hrVCP), and then to establish their in vivo roles in a renal I/R injury model

A case of miliary tuberculosis in a Holestein Fresian dairy cow, Mekelle, Tigray, Ethiopia

We report a case of miliary tuberculosis (TB) found in one of the commercial dairy farms in Mekelle, Tigray, Ethiopia. The case history of the cow indicated weight loss, emaciation and chronic cough. Antibiotic treatment was not successful and there was also repeated death of cows in the farm. Postmortem (PM) examination showed tuberculous lesions in the lung, liver, mediastinal lymphnodes, mesenteric lymphnodes, pleural cavity, reproductive tract, lymphnodes of the head and bone marrow suggesting miliary form of TB. This was confirmed by bacteriological examination and histopathology. The farm where the present case was reported is one of the dairy farms in Mekelle city that supplies milk to the community. As the pasteurization facility is weak in the area, it is suspected that this farm may serve as one of the major transmitters of TB in humans. The lack of control policy in the country worsens the occurrence and spread of TB in animals. In this case, the owner of the Farm was advised to conduct tuberculin skin testing and segregate TB positive cows. Moreover, the owner was advised that it is advantageous to slaughter the reactors under the supervision of the local veterinarian so as to reduce spread of bovine TB in the farm.Keywords: Bovine tuberculosis; Dairy cattle; Exotic; Mekelle; Miliary T

Inter simple sequence repeat (ISSR) analysis of Ethiopian white lupine (Lupinus albus L.)

White lupine (Lupinus albus L.) collected from two zones (West Gojjam and Awi) of Amhara region and one zone (Metekel) of Benishangul - Gumuz regional state of Ethiopia were studied using inter simple sequence repeat (ISSR) markers in an attempt to assess the genetic diversity. Four ISSR primers of which three were dinucleotide repeats and one, a penta nucleotide repeat amplified a total of 39 clear and reproducible bands. Both unweighted pair- group method with arithmetic average (UPGMA) phenograms and a neighbor joining (NJ) trees were constructed for the individuals and populations using Jaccard’s similarity coefficient. The dendrogram clearly indicated four distinct groups/populations based on the area of origin. The principal coordinates (PCO) analysis also recovered UPGMA and neighbor joining tree groups, although Amhara region white lupine were intermixed with each other. The genetic diversity among white lupine population considered in the present study indicated that Merawi was the highest (0.223) followed by Addis Kidam, Sekela and Wembera with genetic diversity of 0.198, 0.189 and 0.167, respectively. Generally, Amhara region white lupine (0.203) population shows higher genetic diversity than white lupine population of B-Gumuz region (0.167). Analysis of molecular variance (AMOVA) in both grouping and without grouping revealed larger genetic diversity within the populations (74.6%) than among populations (25.4%). Shannon’s diversity index also confirmed the existence of higher genetic diversity in Amhara region lupine populations than in Benishangul-Gumuz. Furthermore AMOVA demonstrated highly significant (P = 0.00) genetic differences among populations within groups, among groups and within populations. Of the total variation, 64.64% was attributable to within populations, 27.23% to among groups and the least, 8.13% to among populations within groups. Generally, on the basis of samples of 39 bands in the four populations, ISSR was able to reveal moderate to high levels of genetic diversity within and among Ethiopian white lupine population.Keywords: Amhara, Benishangul - Gumuz, Ethiopia, genetic diversity, ISSR, white lupine.Abbreviation: ISSR, Inter simple sequence repeats; UPGMA, unweighted pair- group method with arithmetic average; NJ, neighbor joining; PCO, principal coordinates; AMOVA, analysis of molecular variance; RAPD, random amplified polymorphic DNA; AFLP, amplified fragment length polymorphism

Analisis dan Perancangan Sistem Informasi Perpustakaan SMA Negeri Xyz

Library information system analysis and design for public senior high school XYZ in South Tangerang aims to identify needs of data and information about library operational transaction in the school. The result of requirement analysis was used to build a computerized system supporting the run of library business process. The analysis and design focused on solving the problems, such difficulties in finding book location, book availability, and else. Rational Unified Process was used for the information system development methodology and the result of requirement analysis was documented by using unified modeling language; user interface was also designed. This information system was expected to ease the library in doing its activity by serving the information related to book searching, book availability, book tracking. Besides, it would also fasten the process of book borrowing, book returning, and new book registration

Sistem Informasi Administrasi Rawat Jalan Pada Klinik Mulia Asih Tangerang

Role of Information Technology in the world of business and business is needed in processing the data into a very useful information as well as the Mulia Asih Clinic is located in Tangerang. Clinic is one of the organizations working in the health field. Aiming to serve and provide a public health service that strives to provide the best service to the patient. In the course of its business, the Mulia Asih Clinic is undergoing a business process that does not have the approriate system, ranging from patient registration process, the inspection process, the process of making a statement or report generation. It is less effective so as to allow the data duplicate , or loss of data. The results achieved are able to assist in the processing of data to produce more accurate information and improve the efficiency of time that will be produced at any time when needed , no longer needed the storage of documents , because documents have been stored in the database, the difficulty in making the report manually , can be facilitated and accelerated by the presence of these systems , in the design of this information , physicians can easily obtain data on the patient\u27s medical history patient statu

Suitability of Groundwater Quality for Irrigation with Reference to Hand Dug Wells, Hantebet Catchment, Tigray, Northern Ethiopia

The purpose of the project has been to assess the suitability of groundwater quality for irrigation purpose in the Hantebet catchment (24.4 km2), Tigray region, northern Ethiopia. The total numbers of hand dug wells in the area are 154. Out of these, 110 are functional and the remaining dried out. Stratified and random sampling techniques were utilized to select representative samples of groundwater. Accordingly, twenty groundwater samples were collected from twenty hand dug wells for chemical analysis. Twenty soil samples were also collected from the command area of the hand dug wells from where the groundwater samples were collected. Both groundwater and soil samples were analyzed for Ca2+, Mg2+, K+, Na+, CO3 2- , HCO3 -, Cl-, SO4 2-, and NO3 - besides pH and electrical conductivity (EC). Further, the Sodium Adsorption Ratio (SAR) for the both the groundwater and soil samples and Exchangeable Sodium Percentage (ESP) for the soil samples were also computed. Out of the analyzed 20 groundwater samples, 8 show EC values below 0.7 and the remaining between 0.71 and 1.12 dS/m, and pH values from 6.55 to 7.26. Chloride ion concentrations in groundwater range from 0.435 to1.393 (meq/l); bicarbonate from 5.124 to 9.660 (meq/l); and nitrate (NO3 - N) values below 5 (mg/l) except in one sample that has 5.87 mg/l. In soil samples, EC values range from 1.36 to 4.65 dS/m (at 25°C) (mean 2.487), and pH values range from 6.77 to 7.79 with a mean value 7.20. SAR values are well below 3 in groundwater, except in one sample and in soil it ranges from 0.111-1.571. ESP values in the soil vary from 2.016 to 4.863. The results indicate that the groundwater in general is suitable for irrigation purpose. In the case of soils about 80% of the soil samples indicate no hazard but 20% are saline. The soils are free of sodicity hazards. However, i) to achieve a full yield potential; ii) to sustain it for long period of time; iii) to avoid the possibility of increase in salinity, and iv) to avoid the possibility of occurrence of sodicity and toxicity hazardous in future, proper irrigation scheme is required in the form of crop selection, fertilizer usage and suitable alternative management

Analisis dan Pengembangan Sistem Manajemen Pengetahuan Berbasis Perangkat Lunak Open Source (Studi Kasus)

Penelitian ini akan memaparkan proses identifikasi dan kategorisasi pengetahuan yang di miliki oleh PT XYZ sebagai objek penelitian, dimana Perusahaan ini membutuhkan wadah yang mendukung sharing knowledge untuk pendokumentasian dan pendistribusian pengetahuan yang ada pada Perusahaan tersebut, serta menerapkan sistem open source untuk mengelola pengetahuan secara mandiri dan terpusat. Metode penelitian yang dilakukan adalah dengan Inukshuk KM Model. Metode pengumpulan data yang digunakan adalah wawancara dan observasi. Hasil yang dicapai dari penelitian ini adalah tersedianya wadah untuk menyimpan dan mengelola pengetahuan yang mudah diakses oleh pengguna. Dengan pengimplementasian sistem manajemen pengetahuan ini diharapkan dapat meningkatan kualitas sharing knowledge antar karyawan, serta meningkatan kualitas knowledge content yang terdapat didalam Perusahaan

2-[2-(2-Pyrid­yl)eth­yl]isoindolinium perchlorate

In the title salt, C15H17N2 +·ClO4 −, the isoindoline N atom is protonated and an intra­molecular N—H⋯N hydrogen bond occurs. In the crystal, N—H⋯O and numerous weak C—H⋯O inter­actions occur between the cation and anion. The O atoms of the perchlorate anion are disordered over four sets of sites with occupancies of 0.438 (4), 0.270 (9), 0.155 (8) and 0.138 (5)