Anxiety and Its Impact on Memory

From influences on our memories of common words to even eyewitness testimonies, anxiety can shape our view of the world (Amir et al., 1996). Our research attempted to show a relationship between anxiety and its effects as an impact on memory as is supported by the Theory of Attentional Control (Eysenck & Calvo, 1992). A video from Simons’ and Chabris’ (1999) study on Inattentional Blindness was used to assess whether anxiety is adaptive or maladaptive to functions of our memory. Our study did not find significance regarding the impact of anxiety on memory. However, the relevance and interest of studies on memory and arousal continues to escalate through the years

Gender and Perception of Music Genre in College Students

Sixty-nine college students listed five songs that they enjoy, then classified each song into one of 15 music genre options. Each of the listed songs were assessed for vocalist gender, which was compared to their music genre classifications and the listeners’ gender. Male vocalists were dominant in every genre tested, outnumbering female vocalists 3.7 to 1. Pop was the notably more equitable exception with a ratio of 1.15:1. However, female vocalists were constricted to the pop genre, as 49% of the listed songs with female vocalists were considered to be pop. Additionally, the study found male and female vocalists have demographically different audiences. It also found some gendered music genres and significant gender differences in the listeners\u27 music genre perception

Forearm design for a myoelectric prosthetic hand

Due to the rapid growth of children and the complexity of myoelectric technology, children are often not given the same opportunities to use myoelectric prosthetics as adults. The Muscle Activated Prosthesis (MAP) team is working to create an affordable, transradial myoelectric prosthesis for a twelve-year-old girl. The basic mechanism by which this device operates is as follows: a muscle contraction emits an electrical signal that will be detected and processed through a microcontroller. Then the onboard software determines whether the hand opens or closes based on the level of muscle intensity. If the software determines to close or open the hand, a signal from the microcontroller is sent to linear actuators that control the tendon system running through the fingers. Currently the team has a working prototype that we plan to give to our client in the fall of 2020 to test.https://mosaic.messiah.edu/engr2020/1016/thumbnail.jp

Chlorophyll enchancement and mixing associated with meanders of the shelf break front in the Mid-Atlantic Bight

Meanders of the shelf break front in the Mid-Atlantic Bight (MAB) during April and May of 1997 were associated with chlorophyll enhancement along a hydrographic and a topographic feature. The hydrographic feature was the surface outcrop of the front, which ranged from ~10 to >100 km seaward of the shelf break owing to the meanders. The topographic feature was the shelf break (100-m isobath). Chlorophyll enhancement was observed by a satellite instrument, the ocean color and temperature sensor, and by a fluorometer in situ. It developed in near-surface waters typically nutrient depleted during late spring, thus local nutrient enrichment of near-surface waters was probable. Observations of sufficient resolution to define processes were available only for the region of shelf break chlorophyll enhancement. Along two meander troughs( shoreward extremities near the shelf break), we observed shoaling of cold shelf water. Shelf water shoaled >20 m along frontal-isopycnals, and phytoplankton absorption maxima coincided directly with the shoaled water. Thus local nutrient enrichment by along-isopycnal upwelling was the supported mechanism of chlorophyll enhancement at the shelf break. The basis for along-isopycnal upwelling was seaward flow of shelf water forced by meander circulation near the shelf break. Strong cross-isobath flow and mixing developed as these meanders propagated along the shelf break front of the MAB at a relatively constant rate of ~9 km day¯¹

Restructuring of the Gut Microbiome by Intermittent Fasting Prevents Retinopathy and Prolongs Survival in db/db Mice

Intermittent fasting (IF) protects against the development of metabolic diseases and cancer, but whether it can prevent diabetic microvascular complications is not known. In db/db mice, we examined the impact of long-term IF on diabetic retinopathy (DR). Despite no change in glycated hemoglobin, db/db mice on the IF regimen displayed significantly longer survival and a reduction in DR end points, including acellular capillaries and leukocyte infiltration. We hypothesized that IF-mediated changes in the gut microbiota would produce beneficial metabolites and prevent the development of DR. Microbiome analysis revealed increased levels of Firmicutes and decreased Bacteroidetes and Verrucomicrobia. Compared with db/db mice on ad libitum feeding, changes in the microbiome of the db/db mice on IF were associated with increases in gut mucin, goblet cell number, villi length, and reductions in plasma peptidoglycan. Consistent with the known modulatory effects of Firmicutes on bile acid (BA) metabolism, measurement of BAs demonstrated a significant increase of tauroursodeoxycholate (TUDCA), a neuroprotective BA, in db/db on IF but not in db/db on AL feeding. TGR5, the TUDCA receptor, was found in the retinal primary ganglion cells. Expression of TGR5 did not change with IF or diabetes. However, IF reduced retinal TNF-α mRNA, which is a downstream target of TGR5 activation. Pharmacological activation of TGR5 using INT-767 prevented DR in a second diabetic mouse model. These findings support the concept that IF prevents DR by restructuring the microbiota toward species producing TUDCA and subsequent retinal protection by TGR5 activation

The Exocyst Protein Sec10 Interacts with Polycystin-2 and Knockdown Causes PKD-Phenotypes

Autosomal dominant polycystic kidney disease (ADPKD) is characterized by formation of renal cysts that destroy the kidney. Mutations in PKD1 and PKD2, encoding polycystins-1 and -2, cause ADPKD. Polycystins are thought to function in primary cilia, but it is not well understood how these and other proteins are targeted to cilia. Here, we provide the first genetic and biochemical link between polycystins and the exocyst, a highly-conserved eight-protein membrane trafficking complex. We show that knockdown of exocyst component Sec10 yields cellular phenotypes associated with ADPKD, including loss of flow-generated calcium increases, hyperproliferation, and abnormal activation of MAPK. Sec10 knockdown in zebrafish phenocopies many aspects of polycystin-2 knockdown—including curly tail up, left-right patterning defects, glomerular expansion, and MAPK activation—suggesting that the exocyst is required for pkd2 function in vivo. We observe a synergistic genetic interaction between zebrafish sec10 and pkd2 for many of these cilia-related phenotypes. Importantly, we demonstrate a biochemical interaction between Sec10 and the ciliary proteins polycystin-2, IFT88, and IFT20 and co-localization of the exocyst and polycystin-2 at the primary cilium. Our work supports a model in which the exocyst is required for the ciliary localization of polycystin-2, thus allowing for polycystin-2 function in cellular processes

Molecular Phylogeny and Biogeography of the Native Rodents of Madagascar (Muridae: Nesomyinae): A Test of the Single-Origin Hypothesis

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72349/1/j.1096-0031.1999.tb00267.x.pd

Socio-economic differences and health seeking behaviour for the diagnosis and treatment of malaria: a case study of four local government areas operating the Bamako initiative programme in south-east Nigeria

BACKGROUND: Malaria is one of the leading causes of mortality and morbidity in Nigeria. It is not known how user fees introduced under the Bamako Initiative (BI) system affect healthcare seeking among different socio-economic groups in Nigeria for diagnosis and treatment of malaria. Reliable information is needed to initiate new policy thrusts to protect the poor from the adverse effect of user fees. METHODS: Structured questionnaires were used to collect information from 1594 female household primary care givers or household head on their socio-economic and demographic status and use of malaria diagnosis and treatment services. Principal components analysis was used to create a socio-economic status index which was decomposed into quartiles and chi-square for trends was used to calculate for any statistical difference. RESULTS: The study showed that self diagnosis was the commonest form of diagnosis by the respondents. This was followed by diagnosis through laboratory tests, community health workers, family members and traditional healers. The initial choice of care for malaria was a visit to the patent medicine dealers for most respondents. This was followed by visit to the government hospitals, the BI health centres, traditional medicine healers, private clinics, community health workers and does nothing at home. Furthermore, the private health facilities were the initial choice of treatment for the majority with a decline among those choosing them as a second source of care and an increase in the utilization of public health facilities as a second choice of care. Self diagnosis was practiced more by the poorer households while the least poor used the patent medicine dealers and community health workers less often for diagnosis of malaria. The least poor groups had a higher probability of seeking treatment at the BI health centres (creating equity problem in BI), hospitals, and private clinics and in using laboratory procedures. The least poor also used the patent medicine dealers and community health workers less often for the treatment of malaria. The richer households complained more about poor staff attitude and lack of drugs as their reasons for not attending the BI health centres. The factors that encourage people to use services in BI health centres were availability of good services, proximity of the centres to the homes and polite health workers. CONCLUSIONS: Factors deterring people from using BI centres should be eliminated. The use of laboratory services for the diagnosis of malaria by the poor should be encouraged through appropriate information, education and communication which at the long run will be more cost effective and cost saving for them while devising means of reducing the equity gap created. This could be done by granting a properly worked out and implemented fee exemptions to the poor or completely abolishing user fees for the diagnosis and treatment of malaria in BI health centres

Adhesion Failures Determine the Pattern of Choroidal Neovascularization in the Eye: A Computer Simulation Study

Choroidal neovascularization (CNV) of the macular area of the retina is the major cause of severe vision loss in adults. In CNV, after choriocapillaries initially penetrate Bruch's membrane (BrM), invading vessels may regress or expand (CNV initiation). Next, during Early and Late CNV, the expanding vasculature usually spreads in one of three distinct patterns: in a layer between BrM and the retinal pigment epithelium (sub-RPE or Type 1 CNV), in a layer between the RPE and the photoreceptors (sub-retinal or Type 2 CNV) or in both loci simultaneously (combined pattern or Type 3 CNV). While most studies hypothesize that CNV primarily results from growth-factor effects or holes in BrM, our three-dimensional simulations of multi-cell model of the normal and pathological maculae recapitulate the three growth patterns, under the hypothesis that CNV results from combinations of impairment of: 1) RPE-RPE epithelial junctional adhesion, 2) Adhesion of the RPE basement membrane complex to BrM (RPE-BrM adhesion), and 3) Adhesion of the RPE to the photoreceptor outer segments (RPE-POS adhesion). Our key findings are that when an endothelial tip cell penetrates BrM: 1) RPE with normal epithelial junctions, basal attachment to BrM and apical attachment to POS resists CNV. 2) Small holes in BrM do not, by themselves, initiate CNV. 3) RPE with normal epithelial junctions and normal apical RPE-POS adhesion, but weak adhesion to BrM (e.g. due to lipid accumulation in BrM) results in Early sub-RPE CNV. 4) Normal adhesion of RBaM to BrM, but reduced apical RPE-POS or epithelial RPE-RPE adhesion (e.g. due to inflammation) results in Early sub-retinal CNV. 5) Simultaneous reduction in RPE-RPE epithelial binding and RPE-BrM adhesion results in either sub-RPE or sub-retinal CNV which often progresses to combined pattern CNV. These findings suggest that defects in adhesion dominate CNV initiation and progression