CRISPR/Cas9-mediated mutagenesis of CAROTENOID CLEAVAGE DIOXYGENASE 8 in tomato provides resistance against the parasitic weed Phelipanche aegyptiaca.

Broomrapes (Phelipanche aegyptiaca and Orobanche spp.) are obligate plant parasites that cause extreme damage to crop plants. The parasite seeds have strict requirements for germination, involving preconditioning and exposure to specific chemicals strigolactones [SLs] exuded by the host roots. SLs are plant hormones derived from plant carotenoids via a pathway involving the Carotenoid Cleavage Dioxygenase 8 (CCD8). Having no effective means to control parasitic weeds in most crops, and with CRISPR/Cas9 being an effective gene-editing tool, here we demonstrate that CRISPR/Cas9-mediated mutagenesis of the CCD8 gene can be used to develop host resistance to the parasitic weed P. aegyptiaca. Cas9/single guide (sg) RNA constructs were targeted to the second exon of CCD8 in tomato (Solanum lycopersicum L.) plants. Several CCD8Cas9 mutated tomato lines with variable insertions or deletions in CCD8 were obtained with no identified off-targets. Genotype analysis of T1 plants showed that the introduced CCD8 mutations are inherited. Compared to control tomato plants, the CCD8Cas9 mutant had morphological changes that included dwarfing, excessive shoot branching and adventitious root formation. In addition, SL-deficient CCD8Cas9 mutants showed a significant reduction in parasite infestation compared to non-mutated tomato plants. In the CCD8Cas9 mutated lines, orobanchol (SL) content was significantly reduced but total carotenoids level and expression of genes related to carotenoid biosynthesis were increased, as compared to control plants. Taking into account, the impact of plant parasitic weeds on agriculture and difficulty to constitute efficient control methods, the current study offers insights into the development of a new, efficient method that could be combined with various collections of resistant tomato rootstocks

In vivo Observation of Tree Drought Response with Low-Field NMR and Neutron Imaging

Using a simple low-field NMR system, we monitored water content in a livingtree in a greenhouse over two months. By continuously running thesystem, we observed changes in tree water content on a scale of halfan hour. The data showed a diurnal change in water content consistentboth with previous NMR and biological observations. Neutron imaging experiments showthat our NMR signal is primarily due to water being rapidly transported through the plant, and not to other sources of hydrogen, such as water in cytoplasm, or water in cell walls. After accountingfor the role of temperature in the observed NMR signal, we demonstratea change in the diurnal signal behavior due to simulated drought conditionsfor the tree. These results illustrate the utility of our system toperform noninvasive measurements of tree water content outside of a temperature controlled environment

An assessment of quality of home-based HIV counseling and testing performed by lay counselors in a rural sub-district of KwaZulu-Natal, South Africa

HIV counseling and testing (HCT) has been prioritized as one of the prevention strategies for HIV/AIDS, and promoted as an essential tool in scaling up and improving access to treatment, care and support especially in community settings. Home-based HCT (HBHCT) is a model that has consistently been found to be highly acceptable and has improved HCT coverage and uptake in low- and middle-income countries since 2002. It involves trained lay counselors going door-todoor offering pre-test counseling and providing HCT services to consenting eligible household members. Currently, there are few studies reporting on the quality of HBHCT services offered by lay counselors especially in Sub-Saharan Africa, including South Africa. This is a quantitative descriptive sub-study of a community randomized trial (Good Start HBHCT trial) which describes the quality of HBHCT provided by lay counselors. Quality of HBHCT was measured as scores comparing observed practice to prescribed protocols using direct observation. Data were collected through periodic observations of HCT sessions and exit interviews with clients. Counselor quality scores for pre-test counseling and post-test counseling sessions were created to determine the level of quality. For the client exit interviews a continuous score was created to assess how satisfied the clients were with the counseling session. A total of 196 (3%) observational assessments and 406 (6%) client exit interviews were completed. Overall, median scores for quality of counseling and testing were high for both HIV-negative and HIV-positive clients. For exit interviews all 406 (100%) clients had overall satisfaction with the counseling and testing services they received, however 11% were concerned about the counselor keeping their discussion confidential. Of all 406 clients, 393 (96.8%) intended to recommend the service to other people. In ensuring good quality HCT services, ongoing quality assessments are important to monitor quality of HCT after training

Big Data: Managing the Future\u27s Agriculture and Natural Resource Systems

Big Data: Managing the Future\u27s Agriculture and Natural Resource Systems Big data is the incredible flow of information that surrounds each of us, every day. Big data tools identify patterns and habits, not only in research, but in manufacturing, logistics–even ordering items online

Center Pivot Irrigation Handbook (EC3017)

Center pivot irrigation has been the most rapidly expanding form of irrigation in the Central Great Plains and across the United States. The amount of land irrigated with sprinkler, gravity and drip or trickle systems was determined for all states in the USA in the Farm and Ranch Irrigation Survey1 by the USDA in 2012. Results of the survey for the Great Plains States shows that approximately 85% of the land in Nebraska is irrigated with center pivots and that very little land was irrigated with drip systems in 2012 (Figure 1). The percentage of land irrigated with sprinklers in neighboring states was similar to the percentage in Nebraska. Results for Colorado, New Mexico and Wyoming include irrigation from the western slopes of the Rocky Mountains where large surface water projects provide water to farms for gravity irrigation. The percentage of land irrigated with sprinklers is similar to that for Nebraska on the eastern plains of Colorado, New Mexico and Wyoming. Virtually all sprinkler-irrigated land in the Great Plains is by center pivots. With widespread use of pivots, it is important to provide techniques to evaluate if pivots are operating as designed and to develop methods to identify issues in producer fields. In this document, we describe the design procedure for center pivots to illustrate how pivots should operate and discuss issues in center pivot performance that we are observing in producer fields. We also present procedures for selecting sprinkler packages and a checklist of things that can help ensure that the system is operating efficiently

Prolonged Exposure With a World War II Veteran: 60 Years of Guilt and Feelings of Inadequacy

There is extensive empirical support for the use of exposure therapy in treating posttraumatic stress disorder (PTSD); however, there is little documented research on the use of these treatments with older adults. Questions exist regarding the appropriateness of exposure therapy with this population, given that the treatment is intense and often involves autonomic nervous system arousal. The current case study details a course of prolonged exposure (PE) therapy in an 88-year-old, World War II veteran, diagnosed with PTSD. The patient was found to respond remarkably well to the treatment and his posttraumatic stress symptoms decreased to subclinical levels in seven sessions. Concerns regarding the appropriateness of using PE to treat older adults are addressed. </jats:p

Future of Water for Food: Science and Technology Panel

The panel explored key issues and challenges in the science and technology of water management to ensure an adequate food supply for the world. Panelists brought many years of experience and perspectives from different areas of expertise. The panelists gave brief overviews of their subject areas and then responded to questions from the audience

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