Study on Spatial Distribution of Soil Available Microelement in Qujing Tobacco Farming Area, China

AbstractDescriptive analysis characteristics and spatial variation characteristics of soil available microelements were studied based on SPSS and GIS Soil available microelements spatial distribution maps were created with ordinary Kriging method. The results indicate that, 7 available microelements in tobacco soil obey lognormal distribution, all the available microelements were intermediate variability; Anisotropic structure of available microelements of tobacco soil varies evidently, spatial variability of available B was mainly caused by random factors, and others’ spatial variability were caused by structural factors and random factors; Spatial distribution maps show that, available B was widely deficient in tobacco soil of Qujing farming area, ‘lower level’ and ‘low level’ taken 7.74% and 68.20%, respectively available Zn distribution was moderate, only 1.32% of the area lack of Zn, available Cu, available Fe and available Mn were extremely high in the whole extension, available Mo was deficient in part of the region with 28.38%, water soluble Cl was higher than critical value(30mgkg−1)in the most of Qujing farming area, which taken 38.86%

Ellipse Encoding for Arbitrary-Oriented SAR Ship Detection Based on Dynamic Key Points

In recent years, there has been growing interest in developing oriented bounding box (OBB)-based deep learning approaches to detect arbitrary-oriented ship targets in synthetic aperture radar (SAR) images. However, most existing OBB-based detection methods suffer from boundary discontinuity problems for bounding box angle prediction and key point regression challenges. In this article, we present a novel OBB-based detection algorithm that utilizes ellipse encoding to effectively exploit the geometric and scattering properties of ship targets. Specifically, the ship contour is fit by an OBB inscribed ellipse that is encoded as a set of distances between dynamic key points on the bow and target center. By combining the bow angle interval and the decoding process, the negative impact of the boundary discontinuity problem is avoided. In addition, we propose an elliptical Gaussian distribution heatmap and a pooling strategy termed double-peak max-pooling (DPM) to deal with the challenge of separating densely distributed ships in inshore scenes. The former can enhance the heatmap’s ship-side score gap between neighboring ship targets, while the latter can solve the problem of target center responses being suppressed after max-pooling. Simulation experiments conducted on the benchmark Rotating SAR Ship Detection Dataset (RSSDD) and Rotated Ship Detection Dataset in SAR Images (RSDD-SAR) demonstrate the superior performance of our method for ship target detection compared to several state-of-the-art OBB-based algorithms. Ablation experiments show that elliptical Gaussian distribution heatmap and DPM can further improve the inshore detection performance

Ellipse Encoding for Arbitrary-Oriented SAR Ship Detection Based on Dynamic Key Points

In recent years, there has been growing interest in developing oriented bounding box (OBB)-based deep learning approaches to detect arbitrary-oriented ship targets in synthetic aperture radar (SAR) images. However, most existing OBB-based detection methods suffer from boundary discontinuity problems for bounding box angle prediction and key point regression challenges. In this article, we present a novel OBB-based detection algorithm that utilizes ellipse encoding to effectively exploit the geometric and scattering properties of ship targets. Specifically, the ship contour is fit by an OBB inscribed ellipse that is encoded as a set of distances between dynamic key points on the bow and target center. By combining the bow angle interval and the decoding process, the negative impact of the boundary discontinuity problem is avoided. In addition, we propose an elliptical Gaussian distribution heatmap and a pooling strategy termed double-peak max-pooling (DPM) to deal with the challenge of separating densely distributed ships in inshore scenes. The former can enhance the heatmap’s ship-side score gap between neighboring ship targets, while the latter can solve the problem of target center responses being suppressed after max-pooling. Simulation experiments conducted on the benchmark Rotating SAR Ship Detection Dataset (RSSDD) and Rotated Ship Detection Dataset in SAR Images (RSDD-SAR) demonstrate the superior performance of our method for ship target detection compared to several state-of-the-art OBB-based algorithms. Ablation experiments show that elliptical Gaussian distribution heatmap and DPM can further improve the inshore detection performance

Extracellular RNA in melanoma: Advances, challenges, and opportunities

Melanoma, a malignant mass lesion that originates in melanocytes and has a high rate of malignancy, metastasis, and mortality, is defined by these characteristics. Malignant melanoma is a kind of highly malignant tumor that produces melanin and has a high mortality rate. Its incidence accounts for 1%–3% of all malignant tumors and shows an obvious upward trend. The discovery of biomolecules for the diagnosis and treatment of malignant melanoma has important application value. So far, the exact molecular mechanism of melanoma development relevant signal pathway still remains unclear. According to previous studies, extracellular RNAs (exRNAs) have been implicated in tumorigenesis and spread of melanoma. They can influence the proliferation, invasion and metastasis of melanoma by controlling the expression of target genes and can also influence tumor progression by participating in signal transduction mechanisms. Therefore, understanding the relationship between exRNA and malignant melanoma and targeting therapy is of positive significance for its prevention and treatment. In this review, we did an analysis of extracellular vesicles of melanoma which focused on the role of exRNAs (lncRNAs, miRNAs, and mRNAs) and identifies several potential therapeutic targets. In addition, we discuss the typical signaling pathways involved in exRNAs, advances in exRNA detection and how they affect the tumor immune microenvironment in melanoma

Toward more accurate variant calling for “personal genomes”

To date, researchers and clinicians use widely different methods for detecting and reporting human genetic variation. As the size of academic and private databases grow and as the use of the existing genomic techniques expand, researchers and clinicians stand to greatly benefit from the standardization of data generating approaches and analysis methodologies. To successfully implement genomic analyses in the clinic, it will be critically important to optimize the existing pipelines for attaining a higher sensitivity and specificity for more accurate and consistent variant calling

Low concordance of multiple variant-calling pipelines: practical implications for exome and genome sequencing

BACKGROUND: To facilitate the clinical implementation of genomic medicine by next-generation sequencing, it will be critically important to obtain accurate and consistent variant calls on personal genomes. Multiple software tools for variant calling are available, but it is unclear how comparable these tools are or what their relative merits in real-world scenarios might be. METHODS: We sequenced 15 exomes from four families using commercial kits (Illumina HiSeq 2000 platform and Agilent SureSelect version 2 capture kit), with approximately 120X mean coverage. We analyzed the raw data using near-default parameters with five different alignment and variant-calling pipelines (SOAP, BWA-GATK, BWA-SNVer, GNUMAP, and BWA-SAMtools). We additionally sequenced a single whole genome using the sequencing and analysis pipeline from Complete Genomics (CG), with 95% of the exome region being covered by 20 or more reads per base. Finally, we validated 919 single-nucleotide variations (SNVs) and 841 insertions and deletions (indels), including similar fractions of GATK-only, SOAP-only, and shared calls, on the MiSeq platform by amplicon sequencing with approximately 5000X mean coverage. RESULTS: SNV concordance between five Illumina pipelines across all 15 exomes was 57.4%, while 0.5 to 5.1% of variants were called as unique to each pipeline. Indel concordance was only 26.8% between three indel-calling pipelines, even after left-normalizing and intervalizing genomic coordinates by 20 base pairs. There were 11% of CG variants falling within targeted regions in exome sequencing that were not called by any of the Illumina-based exome analysis pipelines. Based on targeted amplicon sequencing on the MiSeq platform, 97.1%, 60.2%, and 99.1% of the GATK-only, SOAP-only and shared SNVs could be validated, but only 54.0%, 44.6%, and 78.1% of the GATK-only, SOAP-only and shared indels could be validated. Additionally, our analysis of two families (one with four individuals and the other with seven), demonstrated additional accuracy gained in variant discovery by having access to genetic data from a multi-generational family. CONCLUSIONS: Our results suggest that more caution should be exercised in genomic medicine settings when analyzing individual genomes, including interpreting positive and negative findings with scrutiny, especially for indels. We advocate for renewed collection and sequencing of multi-generational families to increase the overall accuracy of whole genomes

Renal clearable catalytic gold nanoclusters for in vivo disease monitoring

Ultra-small gold nanoclusters (AuNCs) have emerged as agile probes for in vivo imaging, as they exhibit exceptional tumour accumulation and efficient renal clearance properties. However, their intrinsic catalytic activity, which can enable increased detection sensitivity, has yet to be explored for in vivo sensing. By exploiting the peroxidase-mimicking activity of AuNCs and the precise nanometer size filtration of the kidney, we designed multifunctional protease nanosensors that respond to disease microenvironments to produce a direct colorimetric urinary readout of disease state in less than 1 h. We monitored the catalytic activity of AuNCs in collected urine of a mouse model of colorectal cancer where tumour-bearing mice showed a 13-fold increase in colorimetric signal compared to healthy mice. Nanosensors were eliminated completely through hepatic and renal excretion within 4 weeks after injection with no evidence of toxicity. We envision that this modular approach will enable rapid detection of a diverse range of diseases by exploiting their specific enzymatic signatures

Real-time Monitoring for the Next Core-Collapse Supernova in JUNO

Core-collapse supernova (CCSN) is one of the most energetic astrophysical events in the Universe. The early and prompt detection of neutrinos before (pre-SN) and during the SN burst is a unique opportunity to realize the multi-messenger observation of the CCSN events. In this work, we describe the monitoring concept and present the sensitivity of the system to the pre-SN and SN neutrinos at the Jiangmen Underground Neutrino Observatory (JUNO), which is a 20 kton liquid scintillator detector under construction in South China. The real-time monitoring system is designed with both the prompt monitors on the electronic board and online monitors at the data acquisition stage, in order to ensure both the alert speed and alert coverage of progenitor stars. By assuming a false alert rate of 1 per year, this monitoring system can be sensitive to the pre-SN neutrinos up to the distance of about 1.6 (0.9) kpc and SN neutrinos up to about 370 (360) kpc for a progenitor mass of 30$M_{\odot}$ for the case of normal (inverted) mass ordering. The pointing ability of the CCSN is evaluated by using the accumulated event anisotropy of the inverse beta decay interactions from pre-SN or SN neutrinos, which, along with the early alert, can play important roles for the followup multi-messenger observations of the next Galactic or nearby extragalactic CCSN.Comment: 24 pages, 9 figure

Study on the Current Situation of Urban Integration of Aboveground Space and Underground Space: Under the Background of China’s Land Spatial Planning

Urban underground public space has a synergistic effect with its surrounding urban aboveground functions or facilities, which reflects the complementarity between underground functions and urban functions. The research analyses the degree of integration through the case study of urban aboveground space and underground space in China. The research method of this paper will give different evaluation criteria to public transport and public space and the influencing factors of space integration. The indicators of public transport include the number of subway lines and underground parking spaces. The evaluation standard of public space is the area of underground space and the number of floors of underground space. The subway entrance and exits are selected as the evaluation index for the aboveground and underground transition space. Through the specific analysis of 7 selected cases, it provides arguments for the research. The average rent in the case is taken as the dependent variable. Through the regression model, the influencing factors of the integration of aboveground and underground space are determined. The purpose of the study is to explore the influencing factors of the integration of aboveground space and underground space, and how to optimize the integration of aboveground and underground spac

Review of Visualization Technique and Its Application of Road Aggregates Based on Morphological Features

The sustainable performance of asphalt pavement depends on the quality and mix design of road aggregates. Identifying aggregate morphology and size is a prerequisite step for material design and numerical modeling of asphalt mixtures. The paper aims to review the morphometric measurement, characteristic parameters and visualization technique of road aggregates. Types, calculation methods and advantages of aggregate morphological characteristics are highlighted. The applications of aggregate morphological features on the volumetric design, compaction processes, mechanical properties and size effect of asphalt mixtures are summarized. Although digital image processing technology has been studied for years, aggregates in the complex accumulation are still difficult to measure accurately. In the current research, the morphological parameters of aggregates remain diverse without a standard protocol. Compared to theoretical models, numerical models have more difficulties establishing irregular morphology features in the simulated specimens but provide a volume parameter closer to the real value. The future investigation of road performance under dynamic loading should account for the microscopic evolution of shape, orientation and distribution of aggregates over time