Identification and characterization of class 1 integrons among Pseudomonas aeruginosa isolates from patients in Zhenjiang, China

SummaryObjectivesThe role of integrons in the spread of antibiotic resistance has been well established. The aim of this study was to investigate the resistance profiles of Pseudomonas aeruginosa isolated from patients in Zhenjiang to 13 antibiotics, and to identify the structure and dissemination of class 1 integrons.MethodsThe Kirby–Bauer disk diffusion assay was used to determine the rate of P. aeruginosa resistance. Class 1 integrons from multidrug-resistant isolates were amplified by PCR, and their PCR products were sequenced. We also analyzed the integron structures containing the same gene cassettes by restriction fragment length polymorphism (RFLP). Isolates were genotyped by pulsed-field gel electrophoresis (PFGE).ResultsThe resistance rates were between 29.6% and 90.1%. The prevalence of class 1 integrons was 38.0%. These integrons included five gene cassettes (aadB, aac6-II, blaPSE-1, dfrA17, and aadA5). The dfrA17 and aadA5 gene cassettes were found most often.ConclusionsClass 1 integrons were found to be widespread in P. aeruginosa isolated from clinical samples in the Zhenjiang area of China. The antibiotic resistance rates in class 1 integron-positive strains of P. aeruginosa were noticeably higher than those in class 1 integron-negative strains. PFGE showed that particular clones were circulating among patients

Enhanced HMGB1 Expression May Contribute to Th17 Cells Activation in Rheumatoid Arthritis

Rheumatoid arthritis(RA) is a common autoimmune disease associated with Th17 cells, but what about the effect of high-mobility group box chromosomal protein 1 (HMGB1) and the relationship between Th17-associated factors and HMGB1 in RA remains unknown. In the present study, we investigated the mRNA levels of HMGB1, RORγt, and IL-17 in peripheral blood mononuclear cells (PBMCs) from patients with rheumatoid arthritis by quantitative real-time PCR (RT-qPCR), and the concentrations of HMGB1, IL-17, and IL-23 in plasma were detected by ELISA. And then, the effect of HMGB1 on Th17 cells differentiation was analyzed in vitro. Our clinical studies showed that the mRNAs of HMGB1, RORγt, and IL-17 in patients were higher than that in health control (P < 0.05), especially in active RA patients (P < 0.05). The plasma HMGB1, IL-17, and IL-23 in RA patients were also higher than that in health control (P < 0.05); there was a positive correlation between the expression levels of HMGB1 and the amount of CRP, ERS, and RF in plasma. In vitro, the IL-17-produced CD4+T cells were increased with 100 ng/mL rHMGB1 for 12h, which indicated that the increased HMGB1 might contribute to Th17 cells activation in RA patients

Downregulation of Hlx

T-bet plays an important role in immunoregulation; it induces the differentiation of Th1 together with the homeobox transcription factor gene Hlx. Recent studies show that T-bet and Th1-associated factors are critical in regulating tumor development. However, the contributions of Hlx in the occurrence and development of cancer remain unknown. In this study, the Hlx, T-bet, Runx3, and IFN-γ were measured in PBMC from patients with gastric cancer and the correlation between Hlx and T-bet or IFN-γ was assessed. The expression levels of Hlx, T-bet, and IFN-γwere significantly decreased, and there was a positive correlation between Hlx and T-bet or IFN-γ. In addition, the Runx3 expression was also downregulated with the lower T-bet mRNA level. These results suggested that the decreased Hlx expression was closely associated with T-bet and Runx3 downregulations and may contribute to the development of gastric cancer

Genome-Wide Analysis of Protein-Protein Interactions and Involvement of Viral Proteins in SARS-CoV Replication

Analyses of viral protein-protein interactions are an important step to understand viral protein functions and their underlying molecular mechanisms. In this study, we adopted a mammalian two-hybrid system to screen the genome-wide intraviral protein-protein interactions of SARS coronavirus (SARS-CoV) and therefrom revealed a number of novel interactions which could be partly confirmed by in vitro biochemical assays. Three pairs of the interactions identified were detected in both directions: non-structural protein (nsp) 10 and nsp14, nsp10 and nsp16, and nsp7 and nsp8. The interactions between the multifunctional nsp10 and nsp14 or nsp16, which are the unique proteins found in the members of Nidovirales with large RNA genomes including coronaviruses and toroviruses, may have important implication for the mechanisms of replication/transcription complex assembly and functions of these viruses. Using a SARS-CoV replicon expressing a luciferase reporter under the control of a transcription regulating sequence, it has been shown that several viral proteins (N, X and SUD domains of nsp3, and nsp12) provided in trans stimulated the replicon reporter activity, indicating that these proteins may regulate coronavirus replication and transcription. Collectively, our findings provide a basis and platform for further characterization of the functions and mechanisms of coronavirus proteins

Global burden of 288 causes of death and life expectancy decomposition in 204 countries and territories and 811 subnational locations, 1990–2021: a systematic analysis for the Global Burden of Disease Study 2021

BACKGROUND Regular, detailed reporting on population health by underlying cause of death is fundamental for public health decision making. Cause-specific estimates of mortality and the subsequent effects on life expectancy worldwide are valuable metrics to gauge progress in reducing mortality rates. These estimates are particularly important following large-scale mortality spikes, such as the COVID-19 pandemic. When systematically analysed, mortality rates and life expectancy allow comparisons of the consequences of causes of death globally and over time, providing a nuanced understanding of the effect of these causes on global populations. METHODS The Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2021 cause-of-death analysis estimated mortality and years of life lost (YLLs) from 288 causes of death by age-sex-location-year in 204 countries and territories and 811 subnational locations for each year from 1990 until 2021. The analysis used 56 604 data sources, including data from vital registration and verbal autopsy as well as surveys, censuses, surveillance systems, and cancer registries, among others. As with previous GBD rounds, cause-specific death rates for most causes were estimated using the Cause of Death Ensemble model-a modelling tool developed for GBD to assess the out-of-sample predictive validity of different statistical models and covariate permutations and combine those results to produce cause-specific mortality estimates-with alternative strategies adapted to model causes with insufficient data, substantial changes in reporting over the study period, or unusual epidemiology. YLLs were computed as the product of the number of deaths for each cause-age-sex-location-year and the standard life expectancy at each age. As part of the modelling process, uncertainty intervals (UIs) were generated using the 2·5th and 97·5th percentiles from a 1000-draw distribution for each metric. We decomposed life expectancy by cause of death, location, and year to show cause-specific effects on life expectancy from 1990 to 2021. We also used the coefficient of variation and the fraction of population affected by 90% of deaths to highlight concentrations of mortality. Findings are reported in counts and age-standardised rates. Methodological improvements for cause-of-death estimates in GBD 2021 include the expansion of under-5-years age group to include four new age groups, enhanced methods to account for stochastic variation of sparse data, and the inclusion of COVID-19 and other pandemic-related mortality-which includes excess mortality associated with the pandemic, excluding COVID-19, lower respiratory infections, measles, malaria, and pertussis. For this analysis, 199 new country-years of vital registration cause-of-death data, 5 country-years of surveillance data, 21 country-years of verbal autopsy data, and 94 country-years of other data types were added to those used in previous GBD rounds. FINDINGS The leading causes of age-standardised deaths globally were the same in 2019 as they were in 1990; in descending order, these were, ischaemic heart disease, stroke, chronic obstructive pulmonary disease, and lower respiratory infections. In 2021, however, COVID-19 replaced stroke as the second-leading age-standardised cause of death, with 94·0 deaths (95% UI 89·2-100·0) per 100 000 population. The COVID-19 pandemic shifted the rankings of the leading five causes, lowering stroke to the third-leading and chronic obstructive pulmonary disease to the fourth-leading position. In 2021, the highest age-standardised death rates from COVID-19 occurred in sub-Saharan Africa (271·0 deaths [250·1-290·7] per 100 000 population) and Latin America and the Caribbean (195·4 deaths [182·1-211·4] per 100 000 population). The lowest age-standardised death rates from COVID-19 were in the high-income super-region (48·1 deaths [47·4-48·8] per 100 000 population) and southeast Asia, east Asia, and Oceania (23·2 deaths [16·3-37·2] per 100 000 population). Globally, life expectancy steadily improved between 1990 and 2019 for 18 of the 22 investigated causes. Decomposition of global and regional life expectancy showed the positive effect that reductions in deaths from enteric infections, lower respiratory infections, stroke, and neonatal deaths, among others have contributed to improved survival over the study period. However, a net reduction of 1·6 years occurred in global life expectancy between 2019 and 2021, primarily due to increased death rates from COVID-19 and other pandemic-related mortality. Life expectancy was highly variable between super-regions over the study period, with southeast Asia, east Asia, and Oceania gaining 8·3 years (6·7-9·9) overall, while having the smallest reduction in life expectancy due to COVID-19 (0·4 years). The largest reduction in life expectancy due to COVID-19 occurred in Latin America and the Caribbean (3·6 years). Additionally, 53 of the 288 causes of death were highly concentrated in locations with less than 50% of the global population as of 2021, and these causes of death became progressively more concentrated since 1990, when only 44 causes showed this pattern. The concentration phenomenon is discussed heuristically with respect to enteric and lower respiratory infections, malaria, HIV/AIDS, neonatal disorders, tuberculosis, and measles. INTERPRETATION Long-standing gains in life expectancy and reductions in many of the leading causes of death have been disrupted by the COVID-19 pandemic, the adverse effects of which were spread unevenly among populations. Despite the pandemic, there has been continued progress in combatting several notable causes of death, leading to improved global life expectancy over the study period. Each of the seven GBD super-regions showed an overall improvement from 1990 and 2021, obscuring the negative effect in the years of the pandemic. Additionally, our findings regarding regional variation in causes of death driving increases in life expectancy hold clear policy utility. Analyses of shifting mortality trends reveal that several causes, once widespread globally, are now increasingly concentrated geographically. These changes in mortality concentration, alongside further investigation of changing risks, interventions, and relevant policy, present an important opportunity to deepen our understanding of mortality-reduction strategies. Examining patterns in mortality concentration might reveal areas where successful public health interventions have been implemented. Translating these successes to locations where certain causes of death remain entrenched can inform policies that work to improve life expectancy for people everywhere. FUNDING Bill & Melinda Gates Foundation

Comparing incidental vocabulary learning from reading-only and reading-while-listening

This project includes raw and cleaned datasets and R scripts used in the data analyses for the article "Comparing incidental vocabulary learning from reading-only and reading-while-listening" under review in a journal. Below is the abstract: This study compares incidental vocabulary learning in reading-while-listening and reading-only conditions. Using both offline and online outcome measures, I assessed explicit form and meaning knowledge and the lexicalization of new words. I also explored how L2 listening and reading proficiency moderated learning gains in the two treatment conditions. L2 learners first read or read-while-listened to four short stories in English for meaning, with embedded target vocabulary items. They then completed surprise vocabulary posttests in the order of a form priming lexical decision task, a form recognition test, a form-meaning connection test and a semantic priming lexical decision task. Results showed that while the reading-while-listening group outperformed the reading-only group in recognizing the form and meaning of the target vocabulary, neither group fully lexicalized the new words, which was crucial for fluent lexical retrieval. L2 listening and reading proficiency affected learning from reading-while-listening and reading-only differentially: the reading-while-listening group was negatively affected by L2 reading proficiency when controlling for listening proficiency while performance of the reading-only group was not predicted by L2 proficiency. Implications for the use of bimodal input in incidental vocabulary learning are discussed

FACTORS PROMOTING AND HINDERING INFORMATIZATION OF NURSING INSTITUTIONS FOR THE AGED: A TOE THEORETIC PERSPECTIVE

This paper uses the theoretic framework of TOE(technology-organization-environment), identifies the factors promoting and hindering informatization of nursing institutions for the aged. This case study of six nursing institutions for the aged finds that different ownership institutions have some different factors promoting and hindering the process of informatization. In general, compared with public nursing institutions for the aged, private nursing institutions have more both promoting and hindering factors in technological and organizational aspect, but less hindering factors in the environmental aspect; and, risk management is an important promoting factor in the application of information systems for these institutions, it is fewer reported in other industries’ study. These findings extend the application field of the TOE theory; extend the informatization study in nursing institutions for the aged. In practical,we suggest that different ownership’s nursing institutions for the aged should take good advantage of promoting factors considering their own resources, overcome the hindering factors to ensure the successful implementation of the informatization

Distribution and Attribution of Terrestrial Snow Cover Phenology Changes over the Northern Hemisphere during 2001–2020

Snow cover phenology has exhibited dramatic changes in the past decades. However, the distribution and attribution of the hemispheric scale snow cover phenology anomalies remain unclear. Using satellite-retrieved snow cover products, ground observations, and reanalysis climate variables, this study explored the distribution and attribution of snow onset date, snow end date, and snow duration days over the Northern Hemisphere from 2001 to 2020. The latitudinal and altitudinal distributions of the 20-year averaged snow onset date, snow end date, and snow duration days are well represented by satellite-retrieved snow cover phenology matrixes. The validation results by using 850 ground snow stations demonstrated that satellite-retrieved snow cover phenology matrixes capture the spatial variability of the snow onset date, snow end date, and snow duration days at the 95% significance level during the overlapping period of 2001–2017. Moreover, a delayed snow onset date and an earlier snow end date (1.12 days decade−1, p &lt; 0.05) are detected over the Northern Hemisphere during 2001–2020 based on the satellite-retrieved snow cover phenology matrixes. In addition, the attribution analysis indicated that snow end date dominates snow cover phenology changes and that an increased melting season temperature is the key driving factor of snow end date anomalies over the NH during 2001–2020. These results are helpful in understanding recent snow cover change and can contribute to climate projection studies

Utilization of a C-band polarimetric radar for severe rainfall event analysis in complex terrain over eastern China

Polarimetric radar measurements and products perform as the cornerstones of modern severe weather warning and nowcast systems. Two radar quantitative precipitation estimation (QPE) frameworks, one based on a radar-gauge feedback mechanism and the other based on standard rain drop size distribution (DSD)-derived rainfall retrieval relationships, are both evaluated and investigated through an extreme severe convective rainfall event that occurred on 23 June 2015 in the mountainous region over eastern China, using the first routinely operational C-band polarimetric radar in China. Complex rainstorm characteristics, as indicated by polarimetric radar observables, are also presented to account for the severe rainfall field center located in the gap between gauge stations. Our results show that (i) the improvements of the gauge-feedback-derived radar QPE estimator can be attributed to the attenuation correction technique and dynamically adjusted Z-R relationships, but it greatly relies on the gauge measurement accuracy. (ii) A DSD-derived radar QPE estimator based on the specific differential phase (KDP) performs best among all rainfall estimators, and the interaction between the mesocyclone and the windward slope of the mountainous terrain can account for its apparent overestimation. (iii) The rainstorm is mainly dominated by small-sized and moderate-sized raindrops, with the mean volume diameter being less than 2 mm, but its KDP column (KDP &gt; 3°·km-1) has a liquid water content that is higher than 2.4815 g·m-3, and a high raindrop concentration (Nw) with log10(Nw) exceeding 5.1 mm-1m-3. In addition, small hailstones falling and melting are also found in this event, which further aggregates Nw upon the severe rainfall center in the gap between gauge stations.Atmospheric Remote Sensin