Fabrication of three-dimensional microdisk resonators in calcium fluoride by femtosecond laser micromachining

We report on fabrication of on-chip calcium fluoride (CaF2) microdisk resonators using water-assisted femtosecond laser micromachining. Focused ion beam (FIB) milling is used to create ultra-smooth sidewalls. The quality (Q)-factors of the fabricated microresonators are measured to be 4.2x10^4 at wavelengths near 1550 nm. The Q factor is mainly limited by the scattering from the bottom surface of the disk whose roughness remains high due to the femtosecond laser micromachining process. This technique facilitates formation of on-chip microresonators on various kinds of bulk crystalline materials, which can benefit a wide range of applications such as nonlinear optics, quantum optics, and chip-level integration of photonic devices.Comment: 7 pages, 3 figure

Dissolved carbon in a large variety of lakes across five limnetic regions in China

Dissolved carbon in lakes play a vital role in the global carbon cycling. The concentration and dynamics of lake dissolved carbon can be influenced by both the surrounding landscape and a combination of physical, chemical and biological processes within the lakes themselves. From 2009 to 2016, we conducted a large-scale assessment of dissolved organic carbon (DOC) and dissolved inorganic carbon (DIC) in 249 lakes across a diverse range of climatic, geopedologic, topographical and hydrological conditions in five Chinese limnetic regions: the East Limnetic Region (ELR), the Northeast Limnetic Region (NLR), the Inner Mongolia-Xinjiang Limnetic Region (MXR), the Yungui Limnetic Region (YGR), and the Tibet-Qinghai Limnetic Region (TQR). We found that the density of the organic matter in the soil in the surrounding landscape plays an important role in the DOC and DIC in lake water, as was evidenced by the high DOC and DIC levels in the NLR, where the soil is respectively organically rich. Conditions in the arid and semi-arid environments (i.e. TQR and MXR) have created a number of brackish/saline lakes and here we found that, DOC and DIC levels (median: 21.79 and 93.72 mg/L, respectively) are significantly higher than those in the freshwater lakes (median: 5.80 and 29.38 mg/L). It also appears to be the case that the trophic state of freshwater lakes influences the spatial variation of DOC. This can be seen in the relationships between DOC and trophic state index (TSI) in agriculturally-dominated regions such as the ELR (R2 = 0.59, p < 0.01), NLR (R2 = 0.65, p < 0.001), and YGR (R2 = 0.78, p < 0.001). Additionally, a close relationship between DOC and DIC can be found in lake waters with different trophic states (eutrophic: slp = 0.63, R2= 0.69; mesotrophic: slp = 1.03, R2 = 0.65; oligotrophic: slp = 1.00, R2 = 0.64). This indicates that human activities influence the quantity and quality of dissolved carbon in inland water across China. This study is able to provide insights regarding the potential effects of climate change and changes in land-use upon the amount of dissolved carbon in lake water

GSK-3alpha directly regulates beta-adrenergic signaling and the response of the heart to hemodynamic stress in mice.

The glycogen synthase kinase-3 (GSK-3) family of serine/threonine kinases consists of 2 highly related isoforms, alpha and beta. Although GSK-3beta has an important role in cardiac development, much remains unknown about the function of either GSK-3 isoform in the postnatal heart. Herein, we present what we believe to be the first studies defining the role of GSK-3alpha in the mouse heart using gene targeting. Gsk3a(-/-) mice over 2 months of age developed progressive cardiomyocyte and cardiac hypertrophy and contractile dysfunction. Following thoracic aortic constriction in young mice, we observed enhanced hypertrophy that rapidly transitioned to ventricular dilatation and contractile dysfunction. Surprisingly, markedly impaired beta-adrenergic responsiveness was found at both the organ and cellular level. This phenotype was reproduced by acute treatment of WT cardiomyocytes with a small molecule GSK-3 inhibitor, confirming that the response was not due to a chronic adaptation to LV dysfunction. Thus, GSK-3alpha appears to be the central regulator of a striking range of essential processes, including acute and direct positive regulation of beta-adrenergic responsiveness. In the absence of GSK-3alpha, the heart cannot respond effectively to hemodynamic stress and rapidly fails. Our findings identify what we believe to be a new paradigm of regulation of beta-adrenergic signaling and raise concerns given the rapid expansion of drug development targeting GSK-3

Characterization of CDOM in saline and freshwater lakes across China using spectroscopic analysis

Colored dissolved organic matter (CDOM) is a major component of DOM in waters, and plays a vital role in carbon cycling in inland waters. In this study, the light absorption and three-dimensional excitation-emission matrix spectra (EEMs) of CDOM of 936 water samples collected in 2014–2017 from 234 lakes in five regions across China were examined to determine relationships between lake water sources (fresh versus saline) and their fluorescence/absorption characteristics. Results indicated significant differences regarding DOC concentration and aCDOM(254) between freshwater (6.68 mg C L−1, 19.55 m-1) and saline lakes (27.4 mg C L−1, 41.17 m-1). While humic-like (F5) and fulvic-like (F3) compounds contributed to CDOM fluorescence in all lake waters significantly, their contribution to total fluorescence intensity (FT) differed between saline and freshwater lakes. Significant negative relationships were also observed between lake altitude with either F5 (R2 = 0.63, N = 306) or FT (R2 = 0.64, N = 306), suggesting that the abundance of humic-like materials in CDOM tends to decrease with increased in lakes altitude. In high-altitude lakes, strong solar irradiance and UV exposure may have induced photo-oxidation reactions resulting in decreased abundance of humic-like substances and the formation of low molecular weight compounds. These findings have important implications regarding our understanding of C dynamics in lacustrine systems and the contribution of these ecosystems to the global C cycle

Development and validation of a PMA-qPCR method for accurate quantification of viable Lacticaseibacillus paracasei in probiotics

The effectiveness of probiotic products hinges on the viability and precise quantification of probiotic strains. This study addresses this crucial requirement by developing and validating a precise propidium monoazide combination with quantitative polymerase chain reaction (PMA-qPCR) method for quantifying viable Lacticaseibacillus paracasei in probiotic formulations. Initially, species-specific primers were meticulously designed based on core genes from the whole-genome sequence (WGS) of L. paracasei, and they underwent rigorous validation against 462 WGSs, 25 target strains, and 37 non-target strains across various taxonomic levels, ensuring extensive inclusivity and exclusivity. Subsequently, optimal PMA treatment conditions were established using 25 different L. paracasei strains to effectively inhibit dead cell DNA amplification while preserving viable cells. The developed method exhibited a robust linear relationship (R2 = 0.994) between cycle threshold (Cq) values and viable cell numbers ranging from 103 to 108 CFU/mL, with an impressive amplification efficiency of 104.48% and a quantification limit of 7.30 × 103 CFU/mL. Accuracy assessments revealed biases within ±0.5 Log10 units, while Bland–Altman analysis demonstrated a mean bias of 0.058 Log10, with 95% confidence limits of −0.366 to 0.482 Log10. Furthermore, statistical analysis (p = 0.76) indicated no significant differences between theoretical and measured values. This validated PMA-qPCR method serves as a robust and accurate tool for quantifying viable L. paracasei in various sample matrices, including pure cultures, probiotics as food ingredients, and composite probiotic products, thereby enhancing probiotic product quality assurance and contributing to consumer safety and regulatory compliance

Variations in the light absorption coefficients of phytoplankton, non-algal particles and dissolved organic matter in reservoirs across China

Reservoirs were critical sources of drinking water for many large cities around the world, but progress in the development of large-scale monitoring protocols to obtain timely information about water quality had been hampered by the complex nature of inland waters and the various optical conditions exhibited by these aquatic ecosystems. In this study, we systematically investigated the absorption coefficient of different optically-active constituents (OACs) in 120 reservoirs of different trophic states across five eco-regions in China. The relationships were found between phytoplankton absorption coefficient at 675 nm (aph (675)) and Chlorophyll a (Chla) concentration in different regions (R2:0.60-0.82). The non-algal particle (NAP) absorption coefficient (aNAP) showed an increasing trend for reservoirs with trophic states. Significant correlation (p < 0.05) was observed between chromophoric dissolved organic matter (CDOM) absorption and water chemical parameters. The influencing factors for contributing the relative proportion of OACs absorption including the hydrological factors and water quality factors were analyzed. The non-water absorption budget from our data showed the variations of the dominant absorption types which underscored the need to develop and parameterize region-specific bio-optical models for large-scale assessment in water reservoirs

Identification and quantification of viable Lacticaseibacillus rhamnosus in probiotics using validated PMA-qPCR method

The identification and quantification of viable bacteria at the species/strain level in compound probiotic products is challenging now. Molecular biology methods, e.g., propidium monoazide (PMA) combination with qPCR, have gained prominence for targeted viable cell counts. This study endeavors to establish a robust PMA-qPCR method for viable Lacticaseibacillus rhamnosus detection and systematically validated key metrics encompassing relative trueness, accuracy, limit of quantification, linear, and range. The inclusivity and exclusivity notably underscored high specificity of the primers for L. rhamnosus, which allowed accurate identification of the target bacteria. Furthermore, the conditions employed for PMA treatment were fully verified by 24 different L. rhamnosus including type strain, commercial strains, etc., confirming its effective discrimination between live and dead bacteria. A standard curve constructed by type strain could apply to commercial strains to convert qPCR Cq values to viable cell numbers. The established PMA-qPCR method was applied to 46 samples including pure cultures, probiotics as food ingredients, and compound probiotic products. Noteworthy is the congruity observed between measured and theoretical values within a 95% confidence interval of the upper and lower limits of agreement, demonstrating the relative trueness of this method. Moreover, accurate results were obtained when viable L. rhamnosus ranging from 103 to 108 CFU/mL. The comprehensive appraisal of PMA-qPCR performances provides potential industrial applications of this new technology in quality control and supervision of probiotic products

Complete Chloroplast Genome Sequence of a Major Invasive Species, Crofton Weed (Ageratina adenophora)

Crofton weed (Ageratina adenophora) is one of the most hazardous invasive plant species, which causes serious economic losses and environmental damages worldwide. However, the sequence resource and genome information of A. adenophora are rather limited, making phylogenetic identification and evolutionary studies very difficult. Here, we report the complete sequence of the A. adenophora chloroplast (cp) genome based on Illumina sequencing.The A. adenophora cp genome is 150, 689 bp in length including a small single-copy (SSC) region of 18, 358 bp and a large single-copy (LSC) region of 84, 815 bp separated by a pair of inverted repeats (IRs) of 23, 755 bp. The genome contains 130 unique genes and 18 duplicated in the IR regions, with the gene content and organization similar to other Asteraceae cp genomes. Comparative analysis identified five DNA regions (ndhD-ccsA, psbI-trnS, ndhF-ycf1, ndhI-ndhG and atpA-trnR) containing parsimony-informative characters higher than 2%, which may be potential informative markers for barcoding and phylogenetic analysis. Repeat structure, codon usage and contraction of the IR were also investigated to reveal the pattern of evolution. Phylogenetic analysis demonstrated a sister relationship between A. adenophora and Guizotia abyssinica and supported a monophyly of the Asterales.We have assembled and analyzed the chloroplast genome of A. adenophora in this study, which was the first sequenced plastome in the Eupatorieae tribe. The complete chloroplast genome information is useful for plant phylogenetic and evolutionary studies within this invasive species and also within the Asteraceae family

MIHNet: Combining N-gram, Sequential and Global Information for Text Classification

Abstract Pre-training language model has achieved amazing results in many NLP tasks. In Particular, BERT (Bidirectional Encoder Representations from Transformers) create a new era in NLP tasks. Despite the success, these model perform well at Global Information but weak on n-gram and Sequential information. In this paper, we conduct exhaustive experiments of classical text classification models upon BERT in text classification task and provide a general guide for BERT+ models. Finally, we propose a new text classification model called MIHNet (Multi-dimension Information Integration using Highway network), which integrates Global, n-gram and Sequential information together and get a better performance. Notably, our model obtains new state-of-the-art results on eight widely-studied text classification datasets.</jats:p