Production of Martian fiber by in-situ resource utilization strategy

Many countries and commercial organizations have shown great interest in constructing Martian base. In-situ resource utilization (ISRU) provides a cost-effective way to achieve this ambitious goal. In this paper, we proposed to use Martian soil simulant to produce fiber to satisfy material requirement for the construction of Martian base. The composition, melting behavior and fiber forming process of soil simulant was studied, and continuous fiber with a maximum strength of 1320 MPa was obtained on a spinning facility. The findings of this study demonstrate the feasibility of ISRU to prepare Martian fiber from the soil on the Mars, offering a new way to get key materials for the construction of Martian base

Candidate regulators of Early Leaf Development in Maize Perturb Hormone Signalling and Secondary Cell Wall Formation When Constitutively Expressed in Rice

All grass leaves are strap-shaped with a series of parallel veins running from base to tip, but the distance between each pair of veins, and the cell-types that develop between them, differs depending on whether the plant performs C or C photosynthesis. As part of a multinational effort to introduce C traits into rice to boost crop yield, candidate regulators of C leaf anatomy were previously identified through an analysis of maize leaf transcriptomes. Here we tested the potential of 60 of those candidate genes to alter leaf anatomy in rice. In each case, transgenic rice lines were generated in which the maize gene was constitutively expressed. Lines grouped into three phenotypic classes: (1) indistinguishable from wild-type; (2) aberrant shoot and/or root growth indicating possible perturbations to hormone homeostasis; and (3) altered secondary cell wall formation. One of the genes in class 3 defines a novel monocot-specific family. None of the genes were individually sufficient to induce C -like vein patterning or cell-type differentiation in rice. A better understanding of gene function in C plants is now needed to inform more sophisticated engineering attempts to alter leaf anatomy in C plants

H5N1 avian influenza re-emergence of Lake Qinghai: phylogenetic and antigenic analyses of the newly isolated viruses and roles of migratory birds in virus circulation

Highly pathogenic avian influenza H5N1 virus has swept west across the globe and caused serious debates on the roles of migratory birds in virus circulation since the first large-scale outbreak in migratory birds of Lake Qinghai, 2005. In May 2006, another outbreak struck Lake Qinghai and six novel strains were isolated. To elucidate these QH06 viruses, the six isolates were subjected to whole-genome sequencing. Phylogenetic analyses show that QH06 viruses are derived from the lineages of Lake Qinghai, 2005. Five of the six novel isolates are adjacent to the strain A/Cygnus olor/Croatia/1/05, and the last one is related to the strain A/duck/Novosibirsk/02/05, an isolate of the flyway. Antigenic analyses suggest that QH06 and QH05 viruses are similar to each other. These findings implicate that QH06 viruses of Lake Qinghai may travel back via migratory birds, though not ruling out the possibility of local circulation of viruses of Lake Qinghai

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Effectiveness, immunogenicity and safety of one vs. two-dose varicella vaccination：a meta-analysis

<p><b>Background</b>: Despite high 1-dose vaccination coverage, breakthrough varicella infections still occur. Therefore, 2-dose vaccination is recommended to enhance the immune response to the virus. However, the reported incremental vaccine effectiveness (VE) of 2-dose vaccination varies widely among studies.</p> <p><b>Methods</b>: To determine the overall effectiveness, immunogenicity and safety of one vs. two-dose varicella vaccination, we searched five databases for articles published during 1995–2017.</p> <p><b>Results</b>: The incremental VE/efficacy of 2-dose vaccination was 79% (95% confidence interval [CI]: 56%−90%) in randomized controlled trials，63% (95% CI: 36%−79%) in cohort studies and 81% (95% CI: 65%−90%) in case-control studies. Regarding immunogenicity, the pooled standardized mean differences (SMD) of geometric mean titer (GMT) and the pooled relative risk (RR) of seroconversion for 2-dose vs. 1-dose vaccine were 562.44 (95% CI: 471.78–653.10) and 1.03 (95% CI: 1.02–1.04), respectively. Children who received second dose vaccination were at increased risk of grade 3 redness (RR: 4.93, 95% CI: 1.89 − 12.87) and swelling of any intensity (RR: 1.34, 95% CI: 1.08 − 1.66).</p> <p><b>Conclusions</b>: Two-dose varicella vaccination resulted in higher levels of immunogenicity and provided superior protection than 1-dose vaccination. Safety profiles showed 2-dose vaccination was well tolerated but the incidence of grade 3 redness and of any intensity was significantly higher.</p

Genome-scale identification of cell-wall related genes in <it>Arabidopsis</it> based on co-expression network analysis

<p>Abstract</p> <p>Background</p> <p>Identification of the novel genes relevant to plant cell-wall (PCW) synthesis represents a highly important and challenging problem. Although substantial efforts have been invested into studying this problem, the vast majority of the PCW related genes remain unknown.</p> <p>Results</p> <p>Here we present a computational study focused on identification of the novel PCW genes in <it>Arabidopsis</it> based on the co-expression analyses of transcriptomic data collected under 351 conditions, using a bi-clustering technique. Our analysis identified 217 highly co-expressed gene clusters (modules) under some experimental conditions, each containing at least one gene annotated as PCW related according to the Purdue Cell Wall Gene Families database. These co-expression modules cover 349 known/annotated PCW genes and 2,438 new candidates. For each candidate gene, we annotated the specific PCW synthesis stages in which it is involved and predicted the detailed function. In addition, for the co-expressed genes in each module, we predicted and analyzed their <it>cis</it> regulatory motifs in the promoters using our motif discovery pipeline, providing strong evidence that the genes in each co-expression module are transcriptionally co-regulated. From the all co-expression modules, we infer that 108 modules are related to four major PCW synthesis components, using three complementary methods.</p> <p>Conclusions</p> <p>We believe our approach and data presented here will be useful for further identification and characterization of PCW genes. All the predicted PCW genes, co-expression modules, motifs and their annotations are available at a web-based database: <url>http://csbl.bmb.uga.edu/publications/materials/shanwang/CWRPdb/index.html</url>.</p

Highly Efficient Treatment of Oily Sludge by a Novel High-Speed Stirring Method at Room Temperature

Oily sludge is one of the main hazardous wastes which seriously endangers the ecological environment and human health. In this paper, in order to effectively treat oily sludge, a novel high-speed stirring (HSS) method was proposed to clean oily sludge, and the main parameters affecting the residual oil rate of oily sludge were studied experimentally. Firstly, the cleaning time and stirring speed were optimized in the one-stage HSS cleaning, and then the optimal cleaning time of two-stage HSS cleaning was determined by the response surface method. The results suggested that the oil can be efficiently separated by high-speed stirring at room temperature, and that the two-stage cleaning with a circular-hole outlet (Rotor-C) followed by a vertical hole-outlet (Rotor-V) presented the best effect. The optimal stirring speed was 6000 r/min, and the optimal cleaning times of the two-stage cleaning were 7 min and 8 min, respectively. After cleaning, the residual oil rate of the treated oily sludge was 1.65%, and the removal rate of the petroleum hydrocarbons was 84.3%

Effects of berberine on blood glucose in patients with type 2 diabetes mellitus: A systematic literature review and a meta-analysis

We conducted a systematic review and meta-analysis to evaluate the effect of Berberine on glucose in patients with type 2 diabetes mellitus and identify potential factors may modifying the hypoglycemic effect. We searched PubMed, Embase, the Cochrane Library, China National Knowledge Infrastructure, and Wanfang Database to identify randomized controlled trials that investigated the effect of Berberine. We calculated weighted mean differences (WMD) and 95% confidence interval (CI) for fasting plasma glucose (FPG), postprandial plasma glucose (PPG) and glycated haemoglobin (HbA1c) levels. Twenty-eight studies were identified for analysis, with a total of 2,313 type 2 diabetes mellitus (T2DM) patients. The pool data showed that Berberine treatment was associated with a better reduction on FPG (WMD = –0.54 mmol/L, 95% CI: –0.77 to –0.30), PPG (WMD = –0.94 mmol/L, 95% CI: –1.27 to –0.61), and HbA1c (WMD = –0.54 mmol/L, 95% CI: –0.93 to –‍0.15) than control groups. Subgroup-analyses indicated that effects of Berberine on blood glucose became unremarkable as the treatment lasted more than 90 days, the daily dosage more than 2 g/d and patients aged more than 60 years. The efficiency of Berberine combined with hypoglycaemics is better than either Berberine or hypoglycaemic alone. The dosage and treatment duration of Berberine and patients’ age may modify the effect