On the relation between the mass of Compact Massive Objects and their host galaxies

Supermassive black holes and/or very dense stellar clusters are found in the central regions of galaxies. Nuclear star clusters are present mainly in faint galaxies while upermassive black holes are common in galaxies with masses $\geq 10^{10}$ M$_\odot$. In the intermediate galactic mass range both types of central massive objects (CMOs) are found. Here we present our collection of a huge set of nuclear star cluster and massive black hole data that enlarges significantly already existing data bases useful to investigate for correlations of their absolute magnitudes, velocity dispersions and masses with structural parameters of their host galaxies. In particular, we directed our attention to some differences between the correlations of nuclear star clusters and massive black holes as subsets of CMOs with hosting galaxies. In this context, the mass-velocity dispersion relation plays a relevant role because it seems the one that shows a clearer difference between the supermassive black holes and nuclear star clusters. The $M_{MBH}-{\sigma}$ has a slope of $5.19\pm 0.28$ while $M_{NSC}-{\sigma}$ has the much smaller slope of $1.84\pm 0.64$. The slopes of the CMO mass- host galaxy B magnitude of the two types of CMOs are indistinguishable within the errors while that of the NSC mass-host galaxy mass relation is significantly smaller than for supermassive black holes. Another important result is the clear depauperation of the NSC population in bright galaxy hosts, which reflects also in a clear flattening of the NSC mass vs host galaxy mass at high host masses.Comment: 12 pages, 22 figures, 2 tables, accepted for publication in MNRA

BING, a novel antimicrobial peptide isolated from Japanese medaka plasma, targets bacterial envelope stress response by suppressing cpxR expression

Antimicrobial peptides (AMPs) have emerged as a promising alternative to small molecule antibiotics. Although AMPs have previously been isolated in many organisms, efforts on the systematic identification of AMPs in fish have been lagging. Here, we collected peptides from the plasma of medaka (Oryzias latipes) fish. By using mass spectrometry, 6399 unique sequences were identified from the isolated peptides, among which 430 peptides were bioinformatically predicted to be potential AMPs. One of them, a thermostable 13-residue peptide named BING, shows a broad-spectrum toxicity against pathogenic bacteria including drug-resistant strains, at concentrations that presented relatively low toxicity to mammalian cell lines and medaka. Proteomic analysis indicated that BING treatment induced a deregulation of periplasmic peptidyl-prolyl isomerases in gram-negative bacteria. We observed that BING reduced the RNA level of cpxR, an upstream regulator of envelope stress responses. cpxR is known to play a crucial role in the development of antimicrobial resistance, including the regulation of genes involved in drug efflux. BING downregulated the expression of efflux pump components mexB, mexY and oprM in P. aeruginosa and significantly synergised the toxicity of antibiotics towards these bacteria. In addition, exposure to sublethal doses of BING delayed the development of antibiotic resistance. To our knowledge, BING is the first AMP shown to suppress cpxR expression in Gram-negative bacteria. This discovery highlights the cpxR pathway as a potential antimicrobial target

Current Opinions on Optimal Management of Basilar Artery Occlusion: After the BEST of BASICS Survey

Background The best management of basilar artery occlusion (BAO) remains uncertain. The BASICS (Basilar Artery International Cooperation Study) and the BEST (Basilar Artery Occlusion Endovascular Intervention Versus Standard Medical Treatment) trials reported neutral results. We sought to understand physicians’ approaches to BAOs and whether further BAO randomized controlled trials were warranted. Methods We conducted an online international survey from January to March 2022 to stroke neurologists and neurointerventionalists. Survey questions were designed to examine clinical and imaging parameters under which clinicians would offer (or rescind) a patient with BAO to endovascular therapy (EVT) or best medical management versus enrollment into a randomized clinical trial. Results Of >3002 invited participants, 1245 responded (41.4% response rate) from 73 countries, including 54.7% stroke neurologists and 43.6% neurointerventionalists. More than 95% of respondents would offer EVT to patients with BAO, albeit in various clinical circumstances. There were 70.0% of respondents who indicated that the BASICS and BEST trials did not change their practice. Only 22.1% of respondents would perform EVT according to anterior circulation occlusion criteria. The selection of patients for BAO EVT by clinical severity, timing, and imaging modality differed according to geography, specialty, and country income level. Over 80% of respondents agreed that further randomized clinical trials for BAO were warranted. Moreover, 45.6% of respondents indicated they would find it acceptable to enroll all trial‐eligible patients into the medical arm of a BAO trial, whereas 26.3% would not enroll. Conclusion Most stroke physicians continue to believe in the efficacy of EVT in selected patients with BAO in spite of BEST and BASICS. There is no consensus on which selection criteria to use, and few clinicians would use anterior circulation occlusion criteria for BAOs. Further randomized clinical trials for BAO are warranted

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

A multimodal cell census and atlas of the mammalian primary motor cortex

ABSTRACT We report the generation of a multimodal cell census and atlas of the mammalian primary motor cortex (MOp or M1) as the initial product of the BRAIN Initiative Cell Census Network (BICCN). This was achieved by coordinated large-scale analyses of single-cell transcriptomes, chromatin accessibility, DNA methylomes, spatially resolved single-cell transcriptomes, morphological and electrophysiological properties, and cellular resolution input-output mapping, integrated through cross-modal computational analysis. Together, our results advance the collective knowledge and understanding of brain cell type organization: First, our study reveals a unified molecular genetic landscape of cortical cell types that congruently integrates their transcriptome, open chromatin and DNA methylation maps. Second, cross-species analysis achieves a unified taxonomy of transcriptomic types and their hierarchical organization that are conserved from mouse to marmoset and human. Third, cross-modal analysis provides compelling evidence for the epigenomic, transcriptomic, and gene regulatory basis of neuronal phenotypes such as their physiological and anatomical properties, demonstrating the biological validity and genomic underpinning of neuron types and subtypes. Fourth, in situ single-cell transcriptomics provides a spatially-resolved cell type atlas of the motor cortex. Fifth, integrated transcriptomic, epigenomic and anatomical analyses reveal the correspondence between neural circuits and transcriptomic cell types. We further present an extensive genetic toolset for targeting and fate mapping glutamatergic projection neuron types toward linking their developmental trajectory to their circuit function. Together, our results establish a unified and mechanistic framework of neuronal cell type organization that integrates multi-layered molecular genetic and spatial information with multi-faceted phenotypic properties

Simulating inter-city population flows based on graph neural networks

AbstractInter-city population mobility, a critical phenomenon in the modern urbanisation process, is closely related to urban industrial structure and socioeconomic development. This paper aims to investigate the dynamics of population flows and their intricate ties to industrial structure, so we employ the graph neural networks (GNNs) method to simulate inter-city population flows in China, which efficiently integrates demographic and socioeconomic data with Tencent migration big data while accounts for geographical relationships between cities. The results show that the model’s predictive accuracy using the CPC index was high for road and rail traffic and moderate for air transportation. A comparison with real-world data verified the model’s effectiveness in predicting the urban hierarchy and regional aggregation of flows. Using GNNExplainer, the results indicated that population size positively influenced population flow, while developed manufacturing reduced population mobility for road and rail traffic but increased it for air transportation. By conducting scenario simulations in Northeast China, we found that enhancing the region’s industry and consumer service industry could mitigate negative population outflows. The conclusions drawn from this study offer valuable perspectives to policymakers and urban planners, enabling them to make well-informed and judicious choices concerning urban planning, transportation, and resource allocation