165 research outputs found
Leptin and resistin levels in serum of patients with hematologic malignancies: correlation with clinical characteristic
Aim:To evaluate leptin and resistin levels in patients with various hematologic malignancies. Methods: We included 21 patients with lymphoma, 14 with multiple myeloma (MM), 14 with acute leukemia, 13 with chronic lymphocytic leukemia (CLL), and 25 healthy control subjects into our study. The subjectsβ body mass indexes (BMI) were calculated; hematological and acute phase response parameters, serum lipid were determined; serum leptin and resistin levels were determined by ELISA. Results: Serum leptin level was significantly increased in CLL and MM groups when compared to the control group (p < 0.01). Resistin level was significantly higher in lymphoma patients than in CLL, acute leukemia and control groups (p < 0.01). In the control group, leptin level was negatively correlated with hemoglobin level (r = β0.44, p = 0.047); and in all patients with hematologic malignancies, leptin level was correlated with BMI (r = 0.32, p = 0.02). Leptin in lymphoma subjects correlated with hemoglobin level (r = 0.64, p = 0.005), resistin level correlated with the platelet count in patients with hematologic malignancies (r = 0.26, p = 0.044). In addition, leptin level had negative correlations with international prognostic score (IPS) in Hodgkin lymphoma (r = β0.9, p = 0.002) and with international prognostic index (IPI) in non-Hodgkin lymphoma (r = β0.77, p = 0.03). In CLL patients, leptin level had a correlation with the poor prognostic marker β CD38 level (r = 0.68, p = 0.03). Conclusion: We found higher leptin levels in MM and CLL patients, and higher resistin levels in lymphoma patients: this fact demonstrates that changes in adipose tissue and metabolism occur in these disease states.Π¦Π΅Π»Ρ: ΠΎΠΏΡΠ΅Π΄Π΅Π»ΠΈΡΡ ΡΡΠΎΠ²Π½ΠΈ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ Π»Π΅ΠΏΡΠΈΠ½Π° ΠΈ ΡΠ΅Π·ΠΈΡΡΠΈΠ½Π° Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ Π±ΠΎΠ»ΡΠ½ΡΡ
Ρ ΡΠ°Π·Π»ΠΈΡΠ½ΡΠΌΠΈ ΠΎΠ½ΠΊΠΎΠ³Π΅ΠΌΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ
Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡΠΌΠΈ. ΠΠ΅ΡΠΎΠ΄Ρ: ΠΎΠ±ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ 21 Π±ΠΎΠ»ΡΠ½ΠΎΠΉ Π»ΠΈΠΌΡΠΎΠΌΠΎΠΉ, 14 β ΠΌΠ½ΠΎΠΆΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΠΌΠΈΠ΅Π»ΠΎΠΌΠΎΠΉ (ΠΠ), 14 β ΠΎΡΡΡΠΎΠΉ Π»Π΅ΠΉΠΊΠ΅ΠΌΠΈΠ΅ΠΉ,
13 β Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π»ΠΈΠΌΡΠΎΡΠΈΡΠ°ΡΠ½ΠΎΠΉ Π»Π΅ΠΉΠΊΠ΅ΠΌΠΈΠ΅ΠΉ (Π₯ΠΠ), ΠΈ 25 Π·Π΄ΠΎΡΠΎΠ²ΡΡ
Π΄ΠΎΠ½ΠΎΡΠΎΠ². Π£ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Ρ ΡΠ°ΠΊΠΈΠ΅ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠΈ:
ΠΈΠ½Π΄Π΅ΠΊΡ ΠΌΠ°ΡΡΡ ΡΠ΅Π»Π° (ΠΠΠ’), Π³Π΅ΠΌΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΡ, ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ Π»ΠΈΠΏΠΈΠ΄ΠΎΠ² Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ. Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ Π»Π΅ΠΏΡΠΈΠ½Π° ΠΈ ΡΠ΅Π·ΠΈΡΡΠΈΠ½Π°
Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΡΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ: ΡΡΠΎΠ²Π΅Π½Ρ Π»Π΅ΠΏΡΠΈΠ½Π° Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ Π±ΡΠ»
Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎ Π²ΡΡΠ΅ Ρ Π±ΠΎΠ»ΡΠ½ΡΡ
Ρ Π₯ΠΠ ΠΈ ΠΠ, ΡΠ΅ΠΌ ΡΠ°ΠΊΠΎΠ²ΠΎΠΉ Ρ ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΡ (Ρ < 0,01). Π£ΡΠΎΠ²Π΅Π½Ρ ΡΠ΅Π·ΠΈΡΡΠΈΠ½Π° Π±ΡΠ» Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎ
Π²ΡΡΠ΅ Π² Π³ΡΡΠΏΠΏΠ΅ Π±ΠΎΠ»ΡΠ½ΡΡ
Ρ Π»ΠΈΠΌΡΠΎΠΌΠ°ΠΌΠΈ ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ Π₯ΠΠ, ΠΎΡΡΡΠΎΠΉ Π»Π΅ΠΉΠΊΠ΅ΠΌΠΈΠ΅ΠΉ ΠΈ ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΠ°ΠΌΠΈ (Ρ < 0,01). Π ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ
Π³ΡΡΠΏΠΏΠ΅ ΡΡΠΎΠ²Π΅Π½Ρ Π»Π΅ΠΏΡΠΈΠ½Π° ΠΎΡΡΠΈΡΠ°ΡΠ΅Π»ΡΠ½ΠΎ ΠΊΠΎΡΡΠ΅Π»ΠΈΡΠΎΠ²Π°Π» Ρ ΡΡΠΎΠ²Π½Π΅ΠΌ Π³Π΅ΠΌΠΎΠ³Π»ΠΎΠ±ΠΈΠ½Π° (r = β0,44, Ρ = 0,047), Π° Π²ΠΎ Π²ΡΠ΅Ρ
Π³ΡΡΠΏΠΏΠ°Ρ
Π±ΠΎΠ»ΡΠ½ΡΡ
ΡΡΠΎΠ²Π΅Π½Ρ Π»Π΅ΠΏΡΠΈΠ½Π° ΠΊΠΎΡΡΠ΅Π»ΠΈΡΠΎΠ²Π°Π» Ρ ΠΠΠ’ (r = 0,32, Ρ = 0,02). Π£ΡΠΎΠ²Π΅Π½Ρ Π»Π΅ΠΏΡΠΈΠ½Π° ΠΏΡΠΈ Π»ΠΈΠΌΡΠΎΠΌΠ°Ρ
ΠΊΠΎΡΡΠ΅Π»ΠΈΡΠΎΠ²Π°Π» Ρ ΡΡΠΎΠ²Π½Π΅ΠΌ Π³Π΅ΠΌΠΎΠ³Π»ΠΎΠ±ΠΈΠ½Π°
(r = 0,64, Ρ = 0,005), ΡΡΠΎΠ²Π΅Π½Ρ ΡΠ΅Π·ΠΈΡΡΠΈΠ½Π° ΠΊΠΎΡΡΠ΅Π»ΠΈΡΠΎΠ²Π°Π» Ρ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎΠΌ ΡΡΠΎΠΌΠ±ΠΎΡΠΈΡΠΎΠ² Ρ Π±ΠΎΠ»ΡΠ½ΡΡ
Π²ΡΠ΅Ρ
Π³ΡΡΠΏΠΏ (r = 0,26, Ρ = 0,044). ΠΡΠΈ
Π»ΠΈΠΌΡΠΎΠΌΠ΅ Π₯ΠΎΠ΄ΠΆΠΊΠΈΠ½Π° Π²ΡΡΠ²Π»Π΅Π½Π° ΠΎΡΡΠΈΡΠ°ΡΠ΅Π»ΡΠ½Π°Ρ ΠΊΠΎΡΡΠ΅Π»ΡΡΠΈΡ ΠΌΠ΅ΠΆΠ΄Ρ ΡΡΠΎΠ²Π½Π΅ΠΌ Π»Π΅ΠΏΡΠΈΠ½Π° ΠΈ Π²Π΅Π»ΠΈΡΠΈΠ½ΠΎΠΉ ΠΌΠ΅ΠΆΠ΄ΡΠ½Π°ΡΠΎΠ΄Π½ΠΎΠΉ ΠΏΡΠΎΠ³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΎΠΉ
ΡΠΊΠ°Π»Ρ (r = -0,9, Ρ = 0,002), ΠΏΡΠΈ Π½Π΅Ρ
ΠΎΠ΄ΠΆΠΊΠΈΠ½ΡΠΊΠΎΠΉ Π»ΠΈΠΌΡΠΎΠΌΠ΅ β Π²Π΅Π»ΠΈΡΠΈΠ½ΠΎΠΉ ΠΌΠ΅ΠΆΠ΄ΡΠ½Π°ΡΠΎΠ΄Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΠ³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈΠ½Π΄Π΅ΠΊΡΠ° (r = β0,77,
Ρ = 0,03), Ρ Π±ΠΎΠ»ΡΠ½ΡΡ
Π₯ΠΠ β Ρ ΡΡΠΎΠ²Π½Π΅ΠΌ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ CD38 (r = 0,68, Ρ = 0,03). ΠΡΠ²ΠΎΠ΄Ρ: Ρ Π±ΠΎΠ»ΡΠ½ΡΡ
ΠΠ ΠΈ Π₯ΠΠ Π²ΡΡΠ²Π»Π΅Π½ Π²ΡΡΠΎΠΊΠΈΠΉ
ΡΡΠΎΠ²Π΅Π½Ρ Π»Π΅ΠΏΡΠΈΠ½Π°, Π° Ρ Π»ΠΈΠΌΡΠΎΠΌΠ°ΠΌΠΈ β Π²ΡΡΠΎΠΊΠΈΠΉ ΡΡΠΎΠ²Π΅Π½Ρ ΡΠ΅Π·ΠΈΡΡΠΈΠ½Π°: ΡΡΠΎΡ ΡΠ°ΠΊΡ ΡΠΊΠ°Π·ΡΠ²Π°Π΅Ρ Π½Π° ΡΠΎ, ΡΡΠΎ Ρ Π±ΠΎΠ»ΡΠ½ΡΡ
ΡΠΊΠ°Π·Π°Π½Π½ΡΠΌΠΈ ΠΎΠ½ΠΊΠΎΠ³Π΅ΠΌΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ
Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡΠΌΠΈ ΠΌΠΎΠ³ΡΡ Π²ΠΎΠ·Π½ΠΈΠΊΠ°ΡΡ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ Π² ΡΡΡΡΠΊΡΡΡΠ΅ ΠΆΠΈΡΠΎΠ²ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ ΠΈ ΠΎΠ±ΠΌΠ΅Π½Π΅ Π²Π΅ΡΠ΅ΡΡΠ²
Functional biology of the Steel syndrome founder allele and evidence for clan genomics derivation of COL27A1 pathogenic alleles worldwide
Β© 2020, The Author(s). Previously we reported the identification of a homozygous COL27A1 (c.2089G\u3eC; p.Gly697Arg) missense variant and proposed it as a founder allele in Puerto Rico segregating with Steel syndrome (STLS, MIM #615155); a rare osteochondrodysplasia characterized by short stature, congenital bilateral hip dysplasia, carpal coalitions, and scoliosis. We now report segregation of this variant in five probands from the initial clinical report defining the syndrome and an additional family of Puerto Rican descent with multiple affected adult individuals. We modeled the orthologous variant in murine Col27a1 and found it recapitulates some of the major Steel syndrome associated skeletal features including reduced body length, scoliosis, and a more rounded skull shape. Characterization of the in vivo murine model shows abnormal collagen deposition in the extracellular matrix and disorganization of the proliferative zone of the growth plate. We report additional COL27A1 pathogenic variant alleles identified in unrelated consanguineous Turkish kindreds suggesting Clan Genomics and identity-by-descent homozygosity contributing to disease in this population. The hypothesis that carrier states for this autosomal recessive osteochondrodysplasia may contribute to common complex traits is further explored in a large clinical population cohort. Our findings auNorthwell Healthnt our understanding of COL27A1 biology and its role in skeletal development; and expand the functional allelic architecture in this gene underlying both rare and common disease phenotypes
Taming the pandemic? The importance of homemade plant-based foods and beverages as community responses to COVID-19
Household responses to COVID-19 in different corners of the world represent the primary health care that communities have relied on for preventing and mitigating symptoms. During a very complex and confusing time, in which public health services in multiple countries have been completely overwhelmed, and in some cases even collapsed, these first-line household responses have been quintessential for building physical, mental, and social resilience, and for improving individual and community health. This editorial discusses the outcomes of a rapid-response preliminary survey during the first phase of the pandemic among social and community contacts in five metropolises heavily affected by the COVID-19 health crisis (Wuhan, Milan, Madrid, New York, and Rio de Janeiro), and in twelve rural areas or countries initially less affected by the pandemic (Appalachia, Jamaica, Bolivia, Romania, Belarus, Lithuania, Poland, Georgia, Turkey, Pakistan, Cambodia, and South Africa). We summarized our perspectives as 17 case studies, observing that people have relied primarily on teas and spices (βfood-medicinesβ) and that there exist clear international plant favorites, popularized by various new media. Urban diasporas and rural households seem to have repurposed homemade plant-based remedies that they use in normal times for treating the flu and other respiratory symptoms or that they simply consider healthy foods. The most remarkable shift in many areas has been the increased consumption of ginger and garlic, followed by onion, turmeric, and lemon. Our preliminary inventory of food medicines serves as a baseline for future systematic ethnobotanical studies and aims to inspire in-depth research on how use patterns of plant-based foods and beverages, both βtraditionalβ and βnewβ, are changing during and after the COVID-19 pandemic. Our reflections in this editorial call attention to the importance of ethnobiology, ethnomedicine, and ethnogastronomy research into domestic health care strategies for improving community health
Anti-proliferative effect of Rosmarinus officinalis L. extract on human melanoma A375 cells
Rosemary (Rosmarinus officinalis L.) has been used since ancient times in traditional medicine, while nowadays various rosemary formulations are increasingly exploited by alternative medicine to cure or prevent a wide range of health disorders. Rosemary's bioproperties have prompted scientific investigation, which allowed us to ascertain antioxidant, anti-inflammatory, cytostatic, and cytotoxic activities of crude extracts or of pure components. Although there is a growing body of experimental work, information about rosemary's anticancer properties, such as chemoprotective or anti-proliferative effects on cancer cells, is very poor, especially concerning the mechanism of action. Melanoma is a skin tumor whose diffusion is rapidly increasing in the world and whose malignancy is reinforced by its high resistance to cytotoxic agents; hence the availability of new cytotoxic drugs would be very helpful to improve melanoma prognosis. Here we report on the effect of a rosemary hydroalcoholic extract on the viability of the human melanoma A375 cell line. Main components of rosemary extract were identified by liquid chromatography coupled to tandem mass spectrometry (LC/ESI-MS/MS) and the effect of the crude extract or of pure components on the proliferation of cancer cells was tested by MTT and Trypan blue assays. The effect on cell cycle was investigated by using flow cytometry, and the alteration of the cellular redox state was evaluated by intracellular ROS levels and protein carbonylation analysis. Furthermore, in order to get information about the molecular mechanisms of cytotoxicity, a comparative proteomic investigation was performed
Porosity and barrier properties of polyethylene meltblown nonwovens
There are several areas in which textiles have been highly desirable due to their specific and high performance for many industries and sectors including in civil engineering, automobile, medicine, filtration, and electronics. Polymer used has a significant effect on the properties of meltblown nonwovens. Polypropylene being the most commonly used polymer, very limited data have been reported on the polyethylene meltblown nonwovens which are desired due to their unique processing and performance advantages. In this study, we have investigated the effects of process variables, die temperature, air pressure, and die-to-collector distance on some characteristics of polyethylene meltblown nonwovens such as pore size, air permeability, hydrostatic head, and SEM analysis. It was observed that the variables have significant effects on the produced nonwovens, and many of the properties are comparable to those obtained with that of polypropylene meltblown webs. Β© 2016 The Textile Institute
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