Comparative Analysis Of Energy Expenditure Assessments From The Graded Exercise Test Vs. Galaxy Watch And Apple Watch In Korean College Students During A 30-minute Workout: A Pilot Study

OBJECTIVES In the modern era, there is heightened interest in understanding energy expenditure during exercise. Consequently, wearable devices such as the Galaxy Watch and Apple Watch have emerged as pivotal tools for daily health monitoring, given their convenience and increasing popularity. This study aimed to compare the calculated energy expenditure derived from the graded exercise test with readings from Galaxy and Apple Watches during a 30-min exercise session among Korean university students. Through this, we anticipate offering both motivation and clear insights into energy expenditure, thereby potentially aiding in weight management strategies for contemporary individuals. METHODS This study involved 27 college students from Korea National University of Transportation in Chungcheongbuk-do, Korea. We utilized COSMED's exercise load respiratory gas analysis system (Quark-CPET, COSMED, Rome, Italy), along with the Galaxy Watch (Galaxy Watch 5, Samsung, Seoul, Korea) and the Apple Watch (Apple watch series 5, Apple, Cupertino, USA) for measurements. Energy expenditure was monitored in real-time every 5 min throughout the 30-min exercise session. For statistical evaluations, we employed a one-way analysis of variance. Subsequent post-tests utilized the Tukey post-hoc test and Pearson correlation, with a significance level set at p0.05). Conversely, a notable difference was observed when comparing energy expenditure data from the graded exercise test to that of the Apple Watch for time intervals of 10, 15, 20, 25, and 30 min (p>0.05), although the 5-min interval did not exhibit a significant difference (p>0.05). Furthermore, a robust positive correlation was evident between the energy expenditure values derived from the graded exercise test and those from both the Galaxy Watch (r=0.952, p<0.001) and the Apple Watch (r=0.917, p<0.001). CONCLUSIONS Both devices demonstrated high reliability in calculating energy expenditure. Notably, the Galaxy Watch exhibited a more precise calculation compared to the Apple Watch, with a relative reliability margin of 3.5% higher. For individuals, especially those struggling with obesity, precise wearable devices that accurately reflect energy consumption can significantly boost motivation for exercise. Consequently, this study lays a foundation for future advancements in energy expenditure measurement tools, emphasizing enhanced convenience, reliability, and mobility

Probiotics partially attenuate the severity of acute kidney injury through an immunomodulatory effect

Background A healthy microbiome helps maintain the gut barrier and mucosal immune tolerance. Previously, we demonstrated that acute kidney injury (AKI) provoked dysbiosis, gut inflammation, and increased permeability. Here, we investigated the renoprotective effects of the probiotic Bifidobacterium bifidum BGN4 and the underlying mechanisms thereof. Methods C57BL/6 mice were subjected to bilateral renal ischemia-reperfusion injury (IRI) or sham operation. In the probiotic-treated group, BGN4 was administered by gavage once daily, starting 2 weeks before injury. Results Administration of BGN4 significantly increased gut microbiome diversity and prevented expansion of the Enterobacteriaceae and Bacteroidetes that were the hallmarks of AKI-induced dysbiosis. Further, BGN4 administration also significantly reduced other IRI-induced changes in the colon microenvironment, including effects on permeability, apoptosis of colon epithelial cells, and neutrophil and proinflammatory macrophage infiltration. Mononuclear cells co-cultured with BGN4 expressed significantly increased proportions of CD103+/CD11c+ and CD4+ CD25+ Treg cells, suggesting a direct immunomodulatory effect. BGN4 induced Treg expansion in colon, mesenteric lymph nodes (MNL), and kidney. BGN4 also reduced CX3CR1intermediateLy6Chigh monocyte infiltration and interleukin (IL)-17A suppression in the small intestine, which may have attenuated AKI severity, kidney IL-6 messenger RNA expression, and AKI-induced liver injury. Conclusion Prior supplementation with BGN4 significantly attenuated the severity of IRI and secondary liver injury. This renoprotective effect was associated with increased Foxp3 and reduced IL-17A expression in the colon, MNL, and kidney, suggesting that BGN4-induced immunomodulation might contribute to its renoprotective effects. Probiotics may therefore be a promising strategy to reduce AKI severity and/or remote organ injury

Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor

Prime editors, novel genome-editing tools consisting of a CRISPR-Cas9 nickase and an engineered reverse transcriptase, can induce targeted mutagenesis. Nevertheless, much effort is required to optimize and improve the efficiency of prime-editing. Herein, we introduce two strategies to improve the editing efficiency using proximal dead sgRNA and chromatin-modulating peptides. We used enhanced prime-editing to generate Igf2 mutant mice with editing frequencies of up to 47% and observed germline transmission, no off-target effects, and a dwarf phenotype. This improved prime-editing method can be efficiently applied to cell research and to generate mouse models.This study was supported by the Chung Yang, Cha Young Sun, & Jang Hi Joo Memorial Fund. This study was also supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) of Korea (Korea Mouse Phenotyping Project, NRF-2013M3A9D5072550, NRF-2020M3A9D5A01082439, NRF2019R1A2C2087198, and NRF- 2019M3A9H1103792)

Comparison of CPR quality and rescuer fatigue between standard 30:2 CPR and chest compression-only CPR: a randomized crossover manikin trial

Objective We aimed to compare rescuer fatigue and cardiopulmonary resuscitation (CPR) quality between standard 30:2 CPR (ST-CPR) and chest compression only CPR (CO-CPR) performed for 8 minutes on a realistic manikin by following the 2010 CPR guidelines. Methods All 36 volunteers (laypersons; 18 men and 18 women) were randomized to ST-CPR or CO-CPR at first, and then each CPR technique was performed for 8 minutes with a 3-hour rest interval. We measured the mean blood pressure (MBP) of the volunteers before and after performing each CPR technique, and continuously monitored the heart rate (HR) of the volunteers during each CPR technique using the MRx monitor. CPR quality measures included the depth of chest compression (CC) and the number of adequate CCs per minute. Results The adequate CC rate significantly differed between the 2 groups after 2 minutes, with it being higher in the ST-CPR group than in the CO-CPR group. Additionally, the adequate CC rate significantly differed between the 2 groups during 8 minutes for male volunteers (p =0.012). The number of adequate CCs was higher in the ST-CPR group than in the CO-CPR group after 3 minutes (p =0.001). The change in MBP before and after performing CPR did not differ between the 2 groups. However, the change in HR during 8 minutes of CPR was higher in the CO-CPR group than in the ST-CPR group (p =0.007). Conclusions The rate and number of adequate CCs were significantly lower with the CO-CPR than with the ST-CPR after 2 and 6 minutes, respectively, and performer fatigue was higher with the CO-CPR than with the ST-CPR during 8 minutes of CPR.OAIID:RECH_ACHV_DSTSH_NO:220142014020983001RECH_ACHV_FG:RR00200001ADJUST_YN:EMP_ID:A080158CITE_RATE:2.025FILENAME:comparison of cpr quality and rescuer fatigue between st-cpr and cc-only cpr_a randomized crossover manikin trial_scand j trauma resusc emerg med_2014.pdfDEPT_NM:의학과EMAIL:[email protected]_YN:YFILEURL:https://srnd.snu.ac.kr/eXrepEIR/fws/file/d98e73fe-13a9-4bea-b265-cb96af10284f/linkCONFIRM:

Relationships of Mental Disorders and Weight Status in the Korean Adult Population

The purpose of this study was to evaluate the associations between weight status and mental disorders, including depressive disorder, anxiety disorder and alcohol use disorder. A total of nationally representative 6,510 subjects aged 18-64 yr was interviewed in face-to-face household survey. Response rate was 81.7%. Mental disorders were diagnosed using the Korean version of the Composite International Diagnostic Interview (K-CIDI). The subjects reported their heights and weights. After adjusting for age and gender, the lifetime diagnosis of depressive disorder had a significant association with only the underweight group (odds ratio [OR], 1.68, 95% confidence interval [CI], 1.19-2.38). The association between underweight and depressive disorder was the strongest for subjects with a high education level (OR, 1.75, 95% CI, 1.2-2.56), subjects with a married/cohabiting status (OR, 1.94, 95% CI, 1.17-3.22) and smokers (OR, 2.58, 95% CI, 1.33-4.98). There was no significant association between obesity and depressive disorder in Korea. But there was a significant association between the underweight group and depressive disorder. The relationship between obesity and mental disorder in a Korean population was different from that in a Western population. These results suggest that the differences of traditional cultures and races might have an important effect on the associations between the weight status and mental disorders

Targeted Mutagenesis in Mice Using a Base Editor

Base editors, such as cytosine and adenine base editors, are composed of nickase Cas9 (nCas9) and deaminase and serve as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based enzymatic tools for specific nucleotide substitutions. They are mainly the most effective genome editing tools for introducing point mutations, such as C-to-T and A-to-G conversions. The enhanced base editor, a C-to-G base editor (CGBE), can perform other nucleotide substitutions, such as C-to-G conversions. Here, we introduce a method for generating mouse models with point mutations using a base editing system.N