Genetic analysis of farmed and wild stocks of large yellow croaker Larimichthys crocea by using microsatellite markers

The large yellow croaker (Pseudosciaena crocea) is one of the most economically important mariculture fish species in China. In this study, the genetic diversity and relationship among a wild stock, four farmed stocks and a selectively bred strain of large yellow croaker were assessed by 14 microsatellite markers. A total of 108 different alleles were detected over all loci. The average number of allele per locus ranged from 5.57 to 7.93, with an average of 6.75; the observed and expected heterozygosity ranged from 0.572 to 0.665 and from 0.649 to 0.751, with an average of 0.621 and 0.694, respectively; the Shannon’s diversity index ranged from 1.34 to 1.64, with an average of 1.48. The selectively bred strain had the lowest genetic diversity; all farmed stocks showed a slight reduction of genetic variability contrasted with wild stock. All stocks suffered severe bottleneck. The pair-wise FST, the phylogenetic tree, the factor correspondence analysis and the model based clustering analysis revealed that, the Ningbo stock, which was from Zhejiang province, was different from the remaining stocks from Fujian province. This study suggested that (1) the farmed stocks were at relatively low level of genetic diversity compared with the wild stock; (2) samples from Ningbo investigated in this study have a distinct divergence with those from Fujian province; (3) there had emerged significant differentiation among farmed stocks.Key words: Pseudosciaena crocea, large yellow croaker, genetic structure, microsatellite markers

Distributed strain sensor networks for in-construction monitoring and safety evaluation of a high-rise building

2012-2013 > Academic research: refereed > Publication in refereed journalVersion of RecordOthersP0000033, G-U845Publishe

Long-term deformation monitoring of metro-tunnel airshaft excavation during construction stage

Version of RecordPublishe

Studies on the interactions between potassium oxalato-oxodiperoxovanadate and histidine by NMR and MS

Multi-nuclear NMR and ESI-MS have been applied to study the interactions between oxalato-oxodiperoxovanadate and histidine in neutral solution. Coordination between the complex and histidine was monitored by V-51 NMR. A pair of new isomers produced via vanadium atom binding separately to N1 and N3 of the imidazole ring of histidine was characterized by several spectroscopic methods. Experimental results show that the structure activity relationship of peroxovanadium complexes bearing organic ligands may be related to the specific recognition between peroxovanadium and histidine residue of tyrosine phosphatase

Spectroscopic characterization and properties of some bioactive peroxovanadium complexes in aqueous solution

Four bioactive peroxovanadium (pV) complexes-bpV(ox), bpV(bipy), bpV(phen). and bpV(pic), ([VO(O-2)(2)L](n-), where ligand L = oxalic acid dianion (ox), bipyridine(bipy), 1,10-phenanthroline(phen), and pyridine-2-carboxylic acid (pic), were synthesized,and characterized by V-51 NMR, H-1 NMR, C-13 NMR, ESI-MS, IR and elemental analysis. All H-1 and C-13 peaks were,assigned by 2D H-1-H-1 peaks were assigned by 2D H-1-H-1 COSY, HMQC and HMBC. Their stereochemical structures in solution were discussed according to the NMR signals of organic ligands. The descending stability order of complexes in aqueous solution determined by V-51 NMR is bpV(phen), bpV(bipy) bpV (pic) and pV(ox). The predominant decomposition patterns of these complexes were proposed on the basis of electrospray ionization MS (ESI-MS) and V-51 NMR. This work will facilitate the studies of interactions between pV complexes and target biomolecules in solution so as:to clarify structure-function relationship of these:bioactive complexes

Studies on the interactions between bioactive peroxovanadium complexes bearing organic ligands and histidine

In order to explore the structure-activity relationship and molecular mechanism of the specific recognition between peroxovanadium. (pV) complexes bearing organic ligands and the target enzymes of tyrosine phosphatase, several NMR techniques and ESI-MS were used to study the interactions of four pV complexes {pV(ox), pV(bipy), pV(phen) and pV(pic), where pV=[VO(O-2)(2)L](n-), in which L=oxalic acid dianion (ox), bipyridine (bipy), 1, 10-phenanthroline (phen), and pyridine-2-carboxylic acid (pic)} towards histidine, Strong coordination interactions between imidazole of histidine and vanadium of pV(ox) or pV(pic) were observed in neutral solution, while there are not obvious interactions between histidine and pV(bipy) or pV (phen). All C-13 and H-1 NMR signals of 1:1 stoichiometric mixture of pV (ox) and histidine were assigned. Spectroscopic studies demonstrated that new complexes in the mixture of pV (ox) and histidine are a pair of isomers in which the vanadium in pV(ox) binding to the 3-N and 1-N of the imidazole ring. Moreover, the results of effective factors on the interaction system indicated that the new isomers were stable under the condition of physiological pH and the structure-activity relationship of these pV complexes may be relevant to their specific recognition towards histidine residues in tyrosine phosphatase

Nanoscale phase separation of antiferromagnetic order and superconductivity in K0.75_{0.75}Fe1.75_{1.75}Se2_2

We report an in-plane optical spectroscopy study on the iron-selenide superconductor K$_{0.75}$Fe$_{1.75}$Se$_2$. The measurement revealed the development of a sharp reflectance edge below T$_c$ at frequency much smaller than the superconducting energy gap on a relatively incoherent electronic background, a phenomenon which was not seen in any other Fe-based superconductors so far investigated. Furthermore, the feature could be noticeably suppressed and shifted to lower frequency by a moderate magnetic field. Our analysis indicates that this edge structure arises from the development of a Josephson-coupling plasmon in the superconducting condensate. Together with the transmission electron microscopy analysis, our study yields compelling evidence for the presence of nanoscale phase separation between superconductivity and magnetism. The results also enable us to understand various seemingly controversial experimental data probed from different techniques.Comment: 6 figures, published versio

Observation of a ppb mass threshoud enhancement in \psi^\prime\to\pi^+\pi^-J/\psi(J/\psi\to\gamma p\bar{p}) decay

The decay channel $\psi^\prime\to\pi^+\pi^-J/\psi(J/\psi\to\gamma p\bar{p})$ is studied using a sample of $1.06\times 10^8$ $\psi^\prime$ events collected by the BESIII experiment at BEPCII. A strong enhancement at threshold is observed in the $p\bar{p}$ invariant mass spectrum. The enhancement can be fit with an $S$-wave Breit-Wigner resonance function with a resulting peak mass of $M=1861^{+6}_{-13} {\rm (stat)}^{+7}_{-26} {\rm (syst)} {\rm MeV/}c^2$ and a narrow width that is $\Gamma<38 {\rm MeV/}c^2$ at the 90% confidence level. These results are consistent with published BESII results. These mass and width values do not match with those of any known meson resonance.Comment: 5 pages, 3 figures, submitted to Chinese Physics

Functional KV10.1 Channels Localize to the Inner Nuclear Membrane

Ectopically expressed human KV10.1 channels are relevant players in tumor biology. However, their function as ion channels at the plasma membrane does not totally explain their crucial role in tumors. Both in native and heterologous systems, it has been observed that a majority of KV10.1 channels remain at intracellular locations. In this study we investigated the localization and possible roles of perinuclear KV10.1. We show that KV10.1 is expressed at the inner nuclear membrane in both human and rat models; it co-purifies with established inner nuclear membrane markers, shows resistance to detergent extraction and restricted mobility, all of them typical features of proteins at the inner nuclear membrane. KV10.1 channels at the inner nuclear membrane are not all transported directly from the ER but rather have been exposed to the extracellular milieu. Patch clamp experiments on nuclei devoid of external nuclear membrane reveal the existence of channel activity compatible with KV10.1. We hypothesize that KV10.1 channels at the nuclear envelope might participate in the homeostasis of nuclear K+, or indirectly interact with heterochromatin, both factors known to affect gene expression

Lack of relationship between TIMP-1 tumour cell immunoreactivity, treatment efficacy and prognosis in patients with advanced epithelial ovarian cancer

<p>Abstract</p> <p>Background</p> <p>Tissue inhibitor of metalloproteinase 1 (TIMP-1) is a natural inhibitor of the matrix metalloproteinases (MMPs) which are proteolytic enzymes involved in degradation of extracellular matrix thereby favoring tumour cell invasion and metastasis. TIMP-1 activity in tumour tissue may therefore play an essential role in the progression of a malignant tumour.</p> <p>The primary aim of the present study was to evaluate TIMP-1 protein immunoreactivity in tissue from primary ovarian cancer patients and associate these findings with the course of the disease including response to treatment in the individual patient.</p> <p>Methods</p> <p>TIMP-1 was assessed by immunohistochemistry (in tissue micro arrays) in a total of 163 ovarian cancer specimens obtained from primary debulking surgery during 1991-1994 as part of a randomized clinical protocol.</p> <p>Results</p> <p>Positive TIMP-1 immunoreactivity was found in 12.3% of the tumours. The median survival time for the 143 patients with TIMP-1 negative tumours was 23.7 months [19.0-29.4] 95% CI, while the median survival time for the 20 patients with TIMP-1 positive tumours was 15.9 months [12.3-27.4] 95% CI. Although a difference of 7.8 months in median overall survival in favor of the TIMP-1 tumour negative patients was found, this difference did not reach statistical significance (<it>p </it>= 0.28, Kaplan-Meier, log-rank test). Moreover, TIMP-1 immunoreactivity was not associated with CA125 response (p = 0.53) or response at second look surgery (p = 0.72).</p> <p>Conclusion</p> <p>TIMP-1 immunoreactivity in tumour tissue from patients with primary epithelial ovarian cancer did not correlate with patient survival or response to combination platinum/cyclophosphamide therapy.</p