Reducing greenhouse energy consumption using novelty rooftop: a simulation

Recently, more than 80% of total energy of commercial greenhouse in the northern hemisphere is used just for heating. Mostly, the energy loss happens up to 40% caused by the poor U-value of the façades. Therefore, by lowering the U-value would decrease the energy consumption significantly. This simulation is conducted using EnergyPlus software to calculate the heat loss, heating demand and daylighting of a greenhouse with different envelope materials especially novelty rooftop. The orientation of buildings and its effect to electricity generated by semi-transparent PV double glazing are also discussed. In addition, the effect of the novel rooftop to daylighting inside the greenhouse is also investigated. The simulation shows that use materials with low U-value and novel rooftop could decrease the source energy consumption by 65% which is remarkable compared to commercial greenhouse. Besides, the best orientation for the PV module of the greenhouse in Nottingham, UK is facing west-south-east. While the indoor daylighting factor declined up to 65%. Therefore, using PVs with high efficiency would diminish the electricity losses and could be used for lighting energy alternative and others

Epitope Characterization of an Aromatase Monoclonal Antibody Suitable for the Assessment of Intratumoral Aromatase Activity

Immunohistochemistry is one of the most suitable methods for the detection of intratumoral aromatase in order to identify patients who may respond to aromatase inhibitor therapy in hormone-dependent breast cancer. Previous studies showed statistically significant correlation between results of immnuohistochemistry and biochemical analysis in carcinoma components stained by aromatase monoclonal antibody 677. In this study, determination of the antigenic peptides recognized by aromatase antibodies through epitope mapping, combined with the new knowledge on aromatase-reductase interaction, provide insights for understanding various immunostaining patterns using different aromatase antibodies. Our studies on aromatase-reductase interaction also provided critical information on how aromatase and reductase interact with each other on the endoplasmic reticulum membrane, and identified key residues, including K108 of aromatase, that are involved in the interaction with reductase. Through epitope mapping and taking into consideration the interference with aromatase immunohistochemical staining by NADPH-cytochrome P450 reductase, we demonstrated that monoclonal antibody 677 is a suitable antibody for an assessment of intratumoral aromatase activity in breast cancer patients for making clinical management decisions. These results also provide valuable information to identify new aromatase antibodies for immunohistochemical diagnosis of hormone-dependent breast cancer in future

In Search of a Trade Mark: Search Practices and Bureaucratic Poetics

Trade marks have been understood as quintessential ‘bureaucratic properties’. This article suggests that the making of trade marks has been historically influenced by bureaucratic practices of search and classification, which in turn were affected by the possibilities and limits of spatial organisation and technological means of access and storage. It shows how the organisation of access and retrieval did not only condition the possibility of conceiving new trade marks, but also served to delineate their intangible proprietary boundaries. Thereby they framed the very meaning of a trade mark. By advancing a historical analysis that is sensitive to shifts, both in actual materiality and in the administrative routines of trade mark law, the article highlights the legal form of trade mark as inherently social and materially shaped. We propose a historical understanding of trade mark law that regards legal practice and bureaucratic routines as being co-constitutive of the very legal object itself

Bone marrow niche trafficking of miR-126 controls the self-renewal of leukemia stem cells in chronic myelogenous leukemia

Leukemia stem cells (LSCs) in individuals with chronic myelogenous leukemia (CML) (hereafter referred to as CML LSCs) are responsible for initiating and maintaining clonal hematopoiesis. These cells persist in the bone marrow (BM) despite effective inhibition of BCR–ABL kinase activity by tyrosine kinase inhibitors (TKIs). Here we show that although the microRNA (miRNA) miR-126 supported the quiescence, self-renewal and engraftment capacity of CML LSCs, miR-126 levels were lower in CML LSCs than in long-term hematopoietic stem cells (LT-HSCs) from healthy individuals. Downregulation of miR-126 levels in CML LSCs was due to phosphorylation of Sprouty-related EVH1-domain-containing 1 (SPRED1) by BCR–ABL, which led to inhibition of the RAN–exportin-5–RCC1 complex that mediates miRNA maturation. Endothelial cells (ECs) in the BM supply miR-126 to CML LSCs to support quiescence and leukemia growth, as shown using mouse models of CML in which Mir126a (encoding miR-126) was conditionally knocked out in ECs and/or LSCs. Inhibition of BCR–ABL by TKI treatment caused an undesired increase in endogenous miR-126 levels, which enhanced LSC quiescence and persistence. Mir126a knockout in LSCs and/or ECs, or treatment with a miR-126 inhibitor that targets miR-126 expression in both LSCs and ECs, enhanced the in vivo anti-leukemic effects of TKI treatment and strongly diminished LSC leukemia-initiating capacity, providing a new strategy for the elimination of LSCs in individuals with CML

Lieutenant-Colonel John Haughton, commandant of the 36th Sikhs; a hero of Tirah, a memoir,

Mode of access: Internet

England and Russia face to face in Asia;

Mode of access: Internet

Journal of a visit to the Loyalty, New Hebrides, and Banks' Islands : in the Melanesian Mission schooner, the "Southern Cross" ; with an account of the wreck of that vessel /

Also available in an electronic version via the Internet at: http://nla.gov.au/nla.aus-vn761567

Study of nanostructured ultra-refractory Tantalum-Hafnium-Carbide electrodes with wide electrochemical stability window

Transition metal carbides have gathered increasing attention in energy and electrochemistry applications, mainly due to their high structural and physicochemical properties. Their high refractory properties have made them an ideal candidate coating technology and more recently their electronic similarity to the platinum group has expanded their use to energy and catalysis. Here, we demonstrate that the nanostructuring and stoichiometry control of the highest melting point material to this date (Ta-Hf-C) results in outstanding electrochemical stability. Our results show one of the largest windows of stability of a single component electrode in a broad range pH. These experiments provide a new perspective on the electrochemical, thermoelectric and mechanical behavior of Ta-Hf-C nanocomposites, towards a broad range of applications in energy production, catalysis and analytical chemistry.E.C. acknowledges the partial financial support of the National Science Center (NCN) of Poland under the OPUS grant (UMO-2019/35/B/ST5/00248). K.Z. acknowledges the partial financial support from the National Science Centre of Poland by the SONATA Project No. UMO-2016/23/D/ST3/02121. K.S. acknowledges the partial financial support of NCN under the SONATA-BIS grant (2017/26/E/ST5/00416). B.G. and V.B acknowledge the partial financial support of the foundation for polish science (FNP) under the FIRST TEAM program (POIR.04.04.00-00-5D1B/18). Y.K. acknowledges the partial financial support by Basic Science Research Program through the National Research Foundation (NRF) funded by the Ministry of Education (NRF-2017R1A6A1A06015181) of Republic of Korea. J.S.R. and B. D.: acknowledge financial support from the Spanish Ministry of Economy, Industry, and Competitiveness through the “Severo Ochoa” Program for Centers of Excellence in R&D (SEV-2015-0496), and MAT2017-90024-P (TANGENTS)-EI/FEDER.With funding from the Spanishgovernment through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000917-S).Peer reviewe

The Mechanism by Which MYCN Amplification Confers an Enhanced Sensitivity to a PCNA-Derived Cell Permeable Peptide in Neuroblastoma Cells

Dysregulated expression of MYC family genes is a hallmark of many malignancies. Unfortunately, these proteins are not amenable to blockade by small molecules or protein-based therapeutic agents. Therefore, we must find alternative approaches to target MYC-driven cancers. Amplification of MYCN, a MYC family member, predicts high-risk neuroblastoma (NB) disease. We have shown that R9-caPep blocks the interaction of PCNA with its binding partners and selectively kills human NB cells, especially those with MYCN amplification, and we now show the mechanism. We found elevated levels of DNA replication stress in MYCN-amplified NB cells. R9-caPep exacerbated DNA replication stress in MYCN-amplified NB cells and NB cells with an augmented level of MYC by interfering with DNA replication fork extension, leading to Chk1 dependence and susceptibility to Chk1 inhibition. We describe how these effects may be exploited for treating NB