Direct photometric detection of inorganic anions by capillary zone electrophoresis using stacking effect of sulfate ion on sample ions

Stacking effect of sulfate ion on the analysis of inorganic anions by capillary zone electrophoresis was examined during the sample injection period. A used silica capillary was dynamically coated with tetradecyltrimethylammonium bromide (TDTMA(+)Br(-)) to control the electroosmotic flow. Analyte anions were directly detected by photometry at 214 nm. Five kinds of anions, namely bromide, nitrite, nitrate, molybdate, and tungstate, were detected. Anion separation was developed using 4×10(-3) M sodium sulfate in the carrier solution. Peak heights for anions increased along with additional Na(2)SO(4). The stacking effect was more effective for the anions with high mobility than those with low mobility. Calibration graphs for nitrate and nitrite showed good linearity in the concentration range of 10(-6) to 10(-5) M

Calpain chronicle—an enzyme family under multidisciplinary characterization

Calpain is an intracellular Ca2+-dependent cysteine protease (EC 3.4.22.17; Clan CA, family C02) discovered in 1964. It was also called CANP (Ca2+-activated neutral protease) as well as CASF, CDP, KAF, etc. until 1990. Calpains are found in almost all eukaryotes and a few bacteria, but not in archaebacteria. Calpains have a limited proteolytic activity, and function to transform or modulate their substrates’ structures and activities; they are therefore called, “modulator proteases.” In the human genome, 15 genes—CAPN1, CAPN2, etc.—encode a calpain-like protease domain. Their products are calpain homologs with divergent structures and various combinations of functional domains, including Ca2+-binding and microtubule-interaction domains. Genetic studies have linked calpain deficiencies to a variety of defects in many different organisms, including lethality, muscular dystrophies, gastropathy, and diabetes. This review of the study of calpains focuses especially on recent findings about their structure–function relationships. These discoveries have been greatly aided by the development of 3D structural studies and genetic models

An eccentric calpain, CAPN3/p94/calpain-3

AbstractCalpains are Ca2+-regulated proteolytic enzymes that are involved in a variety of biological phenomena. Calpains process substrates by limited proteolysis to modulate various protein functions in the cell, and are thus called “modulator proteases.” CAPN3, previously called p94 or calpain-3, has unique features that are not found in any of the other 14 human calpains, or even in other proteases.For instance, CAPN3 undergoes extremely rapid and exhaustive autodegradation. CAPN3 is also the first (and so far, the only) intracellular enzyme found to depend on Na+ for its activation. CAPN3 has both proteolytic and non-proteolytic functions. It has the interesting distinction of being the only protease, other than a few virus proteases, with the ability to regain protease function after its autolytic dissociation; this occurs through a process known as intermolecular complementation (iMOC). Gene mutations causing CAPN3 defects are responsible for limb-girdle muscular dystrophy type 2A (LGMD2A).Unusual characteristics of CAPN3 have fascinated researchers, but have also hampered conventional biochemical analysis. In this review, we describe significant findings about CAPN3 from its discovery to the present, and suggest promising avenues for future CAPN3 research

El marketing digital y su influencia en el posicionamiento en Cankunapa Restaurante SAC – 2021

La investigación tuvo como objetivo determinar la influencia del marketing digital en el posicionamiento en Cankunapa restaurante S.A.C., 2021; para ello, se siguió una metodología de tipo cuantitativo, cuyo diseño es no experimental correlacional, la técnica que se utilizó es la encuesta y el instrumento es el cuestionario, el mismo que se aplicó a una población de 90 clientes pertenecientes a la empresa objeto de estudio. Dentro de los resultados más importantes se encontró que el marketing digital prevalece en un nivel bajo con un 37%, ya que la empresa no gestiona correctamente las redes sociales, puesto que sólo se basa en la publicidad tradicional a través de la distribución de volantes, tarjetas y folletos; también se encontró que el posicionamiento alcanzó un nivel bajo representado por el 34%, dado que la empresa no tiene un buen posicionamiento en el mercado donde desarrolla sus actividades; asimismo, se identificó que los principales factores del marketing digital que influyen en el posicionamiento es el factor flujo, la funcionalidad y el factor Feedback. Finalmente, se concluyó que el marketing digital influye significativamente en el posicionamiento, evidenciando un coeficiente de 0,674 y un nivel de significación de 0,000, lo que indica que el posicionamiento de la empresa se fortalece en la medida en que se ejerzan acciones basadas en el marketing digital.TesisGestión empresarial y emprendimient

Characterization of hemin-binding protein 35 (HBP35) in Porphyromonas gingivalis: its cellular distribution, thioredoxin activity and role in heme utilization

<p>Abstract</p> <p>Background</p> <p>The periodontal pathogen <it>Porphyromonas gingivalis </it>is an obligate anaerobe that requires heme for growth. To understand its heme acquisition mechanism, we focused on a hemin-binding protein (HBP35 protein), possessing one thioredoxin-like motif and a conserved C-terminal domain, which are proposed to be involved in redox regulation and cell surface attachment, respectively.</p> <p>Results</p> <p>We observed that the <it>hbp35 </it>gene was transcribed as a 1.1-kb mRNA with subsequent translation resulting in three proteins with molecular masses of 40, 29 and 27 kDa in the cytoplasm, and one modified form of the 40-kDa protein on the cell surface. A recombinant 40-kDa HBP35 exhibited thioredoxin activity <it>in vitro </it>and mutation of the two putative active site cysteine residues abolished this activity. Both recombinant 40- and 27-kDa proteins had the ability to bind hemin, and growth of an <it>hbp35 </it>deletion mutant was substantially retarded under hemin-depleted conditions compared with growth of the wild type under the same conditions.</p> <p>Conclusion</p> <p><it>P. gingivalis </it>HBP35 exhibits thioredoxin and hemin-binding activities and is essential for growth in hemin-depleted conditions suggesting that the protein plays a significant role in hemin acquisition.</p

Identification of an O-antigen chain length regulator, WzzP, in Porphyromonas gingivalis

The periodontal pathogen Porphyromonas gingivalis has two different lipopolysaccharides (LPSs) designated O-LPS and A-LPS, which are a conventional O-antigen polysaccharide and an anionic polysaccharide that are both linked to lipid A-cores, respectively. However, the precise mechanisms of LPS biosynthesis remain to be determined. In this study, we isolated a pigment-less mutant by transposon mutagenesis and identified that the transposon was inserted into the coding sequence PGN_2005, which encodes a hypothetical protein of P. gingivalis ATCC 33277. We found that (i) LPSs purified from the PGN_2005 mutant were shorter than those of the wild type; (ii) the PGN_2005 protein was located in the inner membrane fraction; and (iii) the PGN_2005 gene conferred Wzz activity upon an Escherichia coli wzz mutant. These results indicate that the PGN_2005 protein, which was designated WzzP, is a functional homolog of the Wzz protein in P. gingivalis. Comparison of amino acid sequences among WzzP and conventional Wzz proteins indicated that WzzP had an additional fragment at the C-terminal region. In addition, we determined that the PGN_1896 and PGN_1233 proteins and the PGN_1033 protein appear to be WbaP homolog proteins and a Wzx homolog protein involved in LPS biosynthesis, respectively

Pexophagy suppresses ROS-induced damage in leaf cells under high-intensity light

Although light is essential for photosynthesis, it has the potential to elevate intracellular levels of reactive oxygen species (ROS). Since high ROS levels are cytotoxic, plants must alleviate such damage. However, the cellular mechanism underlying ROS-induced leaf damage alleviation in peroxisomes was not fully explored. Here, we show that autophagy plays a pivotal role in the selective removal of ROS-generating peroxisomes, which protects plants from oxidative damage during photosynthesis. We present evidence that autophagy-deficient mutants show light intensity-dependent leaf damage and excess aggregation of ROS-accumulating peroxisomes. The peroxisome aggregates are specifically engulfed by pre-autophagosomal structures and vacuolar membranes in both leaf cells and isolated vacuoles, but they are not degraded in mutants. ATG18a-GFP and GFP-2×FYVE, which bind to phosphatidylinositol 3-phosphate, preferentially target the peroxisomal membranes and pre-autophagosomal structures near peroxisomes in ROS-accumulating cells under high-intensity light. Our findings provide deeper insights into the plant stress response caused by light irradiation

Experimental study on boron distribution and transport at plasma-facing components during impurity powder dropping in the Large Helical Device

Toward real-time wall conditioning, impurity powder dropping experiments with boron powder were performed in the 22nd experimental campaign of the Large Helical Device. To examine the deposition and desorption process of boron, we focus on boron hydride (BH) molecules which presumably populate near plasma-facing components. We performed spatially-resolved spectroscopic measurements of emission by boron ions and BH molecules. From the measurement, we found that BH and B+ were concentrated on the divertor viewing chord, which suggest boron deposition in the divertor region. By comparing Hγ emissions with and without boron injection, neutral hydrogen shows uniform reduction in the SOL region, whereas less reduction of neutral hydrogen is confirmed in the divertor region. Although emissions from BH and B+ increased linearly, emissions by B0 and B4+ became constant after the middle of the discharge. Continuous reduction of carbon density in the core plasma was confirmed even after B0 and B4+ became constant. The results may show reduction of hydrogen recycling and facilitation of impurity gettering by boron in the divertor region and thus effective real-time wall conditioning

看護学部学生の国際的活動に関する意識調査

　本調査の目的は，本学看護学部学生の国際的活動に関する意識や期待，活動の実態を明らかにし，学部における国際的活動の方向性を検討することである．1年生から3年生264名を対象に無記名の自記式質問紙調査を行い回収率は18.9％であった．68％の学生が外国に関心があると回答したが国際交流への関心は54%と若干低かった．国際交流や異文化経験は将来看護職になる上で役立つと考えている学生は90%であり，医療や看護の視点で国際交流や異文化を学ぶことへ前向きであることが明らかになった．国際交流に関心がない理由は，興味がない，語学が苦手，外国が怖いなどがあげられた．本学の海外研修プログラムへの参加を希望しない学生は58%であり，理由は語学，経済，時間的余裕のなさ，関心のなさであった．一方、海外の病院や看護大学訪問の希望があり，海外の医療や看護に関心があることがわかった．これらから，語学，期間，費用，内容が研修参加に関する要因であり，今後の企画に考慮する必要がある．また，学内で文化の多様性を学ぶ機会を設けること，教育や研究における教員と海外研究者との交流を促進し，学生に還元していくことが重要である

糖尿病患者の在宅ケア向上をめざしたTCDS育成の試み

The Tokushima City Medical Association has cultivated the talented persons of nursing care profession by both the education of diabetes and the instruction of medical treatments to secure the quality of home care for increasing diabetic patients. They are certified to be the Tokushima City Certified Diabetes Supporter（TCDS）. In Tokushima Prefecture, the rate of aging and the certification rate of care need are ranked high in Japan, and the medical measures should be provided for the aged diabetic patients utilizing team nursing care as well as team medical care, because many of these patients are obliged to receive home medical care owing to the introduction of community-based integrated care systems. Tokushima Prefecture kept the worst of age-adjusted diabetes mortality and also the worst of crude diabetes mortality in recent years. Therefore, the program for the TCDS was arranged by the staffs composed of board certified fellows of the Japan Diabetes Society, certified diabetes physicians of Tokushima, and Tokushima local certified diabetes educators （Tokushima LCDEs）. The program includes the lectures of diabetes and medical treatments, the practical training, and the group work by the World Café system collaborated with the medical staffs across many different fields, using the dramatic skit presented by the medical doctors and the LCDE staffs after narrating the scenario for the blood glucose control of diabetic patients to be treated. The persons who have completed the training course are certified as the TCDS by The Tokushima City Medical Association. The workshop is held twice a year and the certification is renewed every three years without examination. In conclusion, it is suggested that the development of TCDS leads to the improvement of the ability of nursing care staffs to support diabetic treatments and the advancement of the quality of home medical care for the aged diabetic patients