Focal mechanisms and aftershock locations of the Songpan earthquakes of August 1976 in Sichuan, China

The precursory swarm, three mainshocks (M = 7.2,6.7, 7.2), and aftershocks of the Songpan earthquakes have been reanalyzed using both local and teleseismic data. The three mainshocks of this sequence occurred on the Huya fault over a 7-day period. Relocations of the aftershocks using local arrival times show that three fault strands were activated during this sequence. Each mainshock occurred on a separate strand, each one south of the strand activated in the previous mainshock, and the aftershock zones of each mainshock appear to abut rather than overlap. Fault plane solutions determined by matching teleseismic P waveforms at World-Wide Standard Seismograph Network stations with synthetic seismograms are consistent with the observed aftershock zones. The first and third mainshocks (M_0 = 1.3 ×10^(19) and 8.4 × 10^(18) N m, respectively) showed almost identical senses of motion, a combination of reverse and left-lateral strike-slip motion, on parallel strands, striking N15°W, that were separated by a large rightstepping en echelon offset. The second mainshock (M_0 = 4.0 × 10^(18) N m), occurred in this offset on a fault at a steep angle (∼125°) to the other two strands and showed almost pure reverse motion. Differences in the orientations of the slip vectors of the three mainshocks show that the first mainshock increased the normal and shear stresses on the fault segment that moved in the second mainshock and that the second mainshock decreased the normal stress on the fault segment activated by the third mainshock. These changes in normal stresses may have given rise to the longer time between the first and second events (5 days) as compared with the time between the second and third events (30 hours). A precursory swarm that preceded the Songpan sequence by 3 years occurred in a volume that surrounded the northernmost part of the planar aftershock zone. The time between the start of the swarm and the mainshocks and the magnitude of the largest event in the swarm are similar to those seen for precursory swarms in Soviet Central Asia

A chromosome-level draft genome of the grain aphid Sitobion miscanthi.

peer reviewed[en] BACKGROUND: Sitobion miscanthi is an ideal model for studying host plant specificity, parthenogenesis-based phenotypic plasticity, and interactions between insects and other species of various trophic levels, such as viruses, bacteria, plants, and natural enemies. However, the genome information for this species has not yet to be sequenced and published. Here, we analyzed the entire genome of a parthenogenetic female aphid colony using Pacific Biosciences long-read sequencing and Hi-C data to generate chromosome-length scaffolds and a highly contiguous genome assembly. RESULTS: The final draft genome assembly from 33.88 Gb of raw data was ∼397.90 Mb in size, with a 2.05 Mb contig N50. Nine chromosomes were further assembled based on Hi-C data to a 377.19 Mb final size with a 36.26 Mb scaffold N50. The identified repeat sequences accounted for 26.41% of the genome, and 16,006 protein-coding genes were annotated. According to the phylogenetic analysis, S. miscanthi is closely related to Acyrthosiphon pisum, with S. miscanthi diverging from their common ancestor ∼25.0-44.9 million years ago. CONCLUSIONS: We generated a high-quality draft of the S. miscanthi genome. This genome assembly should help promote research on the lifestyle and feeding specificity of aphids and their interactions with each other and species at other trophic levels. It can serve as a resource for accelerating genome-assisted improvements in insecticide-resistant management and environmentally safe aphid management.National Key R & D Plan of Chin

Functional analysis of odorant-binding proteins for the parasitic host location to implicate convergent evolution between the grain aphid and its parasitoid Aphidius gifuensis.

peer reviewed(E)-β-farnesene (EBF) is a typical and ecologically important infochemical in tri-trophic level interactions among plant-aphid-natural enemies. However, the molecular mechanisms by which parasitoids recognize and utilize EBF are unclear. In this study, we functionally characterized 8 AgifOBPs in Aphidifus gifuensis, one dominant endo-parasitoid of wheat aphid as well as peach aphid in China. Among which, AgifOBP6 was the only OBP upregulated by various doses of EBF, and it showed a strong binding affinity to EBF in vitro. The lack of homology between AgifOBP6 and EBF-binding proteins from aphids or from other aphid natural enemies supported that this was a convergent evolution among insects from different orders driven by EBF. Molecular docking of AgifOBP6 with EBF revealed key interacting residues and hydrophobic forces as the main forces. AgifOBP6 is widely expressed among various antennal sensilla. Furthermore, two bioassays indicated that trace EBF may promote the biological control efficiency of A. gifuensis, especially on winged aphids. In summary, this study reveals an OBP (AgifOBP6) that may play a leading role in aphid alarm pheromone detection by parasitoids and offers a new perspective on aphid biological control by using EBF. These results will improve our understanding of tri-trophic level interactions among plant-aphid-natural enemies.National Key Research and Development Program of ChinaAgricultural Science and Technology Innovation Progra

Free sulfurous acid (FSA) inhibition of biological thiosulfate reduction (BTR) in the sulfur cycle-driven wastewater treatment process

A sulfur cycle-based bioprocess for co-treatment of wet flue gas desulfurization (WFGD) wastes with freshwater sewage has been developed. In this process the removal of organic carbon is mainly associated with biological sulfate or sulfite reduction. Thiosulfate is a major intermediate during biological sulfate/sulfite reduction, and its reduction to sulfide is the rate-limiting step. In this study, the impacts of saline sulfite (the ionized form: HSO + SO ) and free sulfurous acid (FSA, the unionized form: HSO) sourced from WGFD wastes on the biological thiosulfate reduction (BTR) activities were thoroughly investigated. The BTR activity and sulfate/sulfite-reducing bacteria (SRB) populations in the thiosulfate-reducing up-flow anaerobic sludge bed (UASB) reactor decreased when the FSA was added to the UASB influent. Batch experiment results confirmed that FSA, instead of saline sulfite, was the true inhibitor of BTR. And BTR activities dropped by 50% as the FSA concentrations were increased from 8.0 × 10to 2.0 × 10mg HSO-S/L. From an engineering perspective, the findings of this study provide some hints on how to ensure effective thiosulfate accumulation in biological sulfate/sulfite reduction for the subsequent denitrification/denitritation. Such manipulation would result in higher nitrogen removal rates in this co-treatment process of WFGD wastes with municipal sewage

Kairomonal Effect of Aphid Alarm Pheromones and Analogs on the Parasitoid Diaeretiella rapae

Aphid alarm pheromones, as important semiochemicals, not only mediate behavioral response of aphids, but can also act as kairomones to attract their natural enemies. The sesquiterpene (E)-β-farnesene (EβF), the major alarm pheromone component of most aphid species, has been shown to have a kairomonal effect on the predators of aphids, but other alarm pheromone components, especially the monoterpenes and analogs, are rarely investigated. Here, two EβF analogs were successfully synthesized via the nucleophilic substitution reaction, and we then examined the kairomonal effects of four alarm pheromone components and two EβF analogs on the aphid parasitoid, Diaeretiella rapae. In olfactory bioassays, D. rapae females generally showed no significant behavioral response to these alarm pheromone components and analogs under low concentrations (0.1 μg/μL). Nevertheless, their olfactory response to these compounds gradually enhanced with increasing concentrations. Among the four pheromone components, EβF showed the highest attractive activity, but the parasitoid preferred blends over single compounds. Moreover, the response time decreased as the concentration increased. We confirmed the kairomonal effect of monoterpene alarm pheromone components and their blends, in addition to EβF, on the natural enemies of aphids. This is the first report that the blend of alarm pheromone components and their analogs has a stronger kairomonal effect than do the single components on the natural enemies of aphids. This study contributes to our understanding of the mechanisms involved in the regulation of parasitoid behaviors by kairomones and provides a promising opportunity for designing kairomones for the aphid parasitoid to mediate aphid populations in the field

Grey Accumulation Generation Relational Analysis Model for Nonequidistance Unequal-Length Sequences and Its Application

As research is required on nonequidistance unequal-length sequences, so grey accumulation generation relational analysis model based on grey exponential law (AGRA) for nonequidistance unequal-length (NDUL) sequences is put forward in this paper. The original data is accumulated generation firstly and the generation sequences are simulated. Then the generation rate is established as the ratio of the tangent slope and the mean of the simulation function. Furthermore, the dynamic similarity of change trend of the original time sequences is characterized by the proximity of generation rate sequences. Meanwhile, properties of AGRA model for nonequidistance unequal-length sequences are discussed. The new relational analysis model is available for equal interval sequences, nonequidistance sequences, sequences which have relationship before transformation and sequences which have relationship after accumulation; therefore, the AGRA model has expanded the scope of application of grey relational analysis. Lastly, factors which affect the amount of passenger cars in China are sorted using AGRA model for NDUL sequences. This application is presented to illustrate the effectiveness and practicality of the proposed model

Study on the influence of electrode materials on energy storage power station in lithium battery

Lithium batteries are promising techniques for renewable energy storage attributing to their excellent cycle performance, relatively low cost, and guaranteed safety performance. The performance of the LiFePO4 (LFP) battery directly determines the stability and safety of energy storage power station operation, and the properties of the internal electrode materials are the core and key to determine the quality of the battery. In this work, two kinds of commercial LFP batteries were studied by analyzing the electrical properties and material properties of the electrodes. After performing the rate and cycle performance tests of the battery cell, differential scanning calorimetry, scanning electron microscope, x-ray diffraction, and inductively coupled plasma were used to explore the performance of electrode materials to establish the correlation between the battery performance and the electrochemical reaction law of the internal materials and further to judge the influence of the key material properties on the battery performance. This work opens up an avenue for technical supervision of energy storage power stations

Pathogenic Implication of a Fibrinogen-Binding Protein of Staphylococcus epidermidis in a Rat Model of Intravascular-Catheter-Associated Infection▿

The involvement of Fbe, a fibrinogen-binding protein of Staphylococcus epidermidis, in the pathogenesis of catheter-associated infection was investigated. An fbe (gene encoding Fbe protein) mutant was constructed by allelic replacement, wherein an erythromycin resistance gene replaced a portion of the A region of fbe. Meanwhile, a rat central venous catheter (CVC) infection model was established to assess the importance of Fbe in the pathogenesis of CVC-associated infection due to S. epidermidis. Fbe-positive S. epidermidis strain HB was significantly more likely to cause a CVC-associated infection resulting in bacteremia and metastatic disease than its isogenic Fbe-deficient mutant (100% versus 20%, P < 0.01). These results confirm the importance of adherence associated with Fbe in the pathogenesis of CVC-associated infection caused by S. epidermidis