ADENOSINE TRIPHOSPHATASE ACTIVITY OF THE VENOUS WALL IN CAT

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Marine fishes in the Black Sea: recent conservation status

A revised checklist of the fish fauna of the Black Sea is reported. This paper is the first attempt to present an actual Check List of the fishes in the Black Sea according to the data available in the Black Sea countries, as well as their current conservation status, enlisted in IUCN. The total number of the Black Sea fish species is 189. Concerning the conservation status only two species (1.06 %) are extinct (Acipenser nudiventris and A. sturio), 3.70 % are critically endangered, 16.40 % are vulnerable, 1.06% are endangered, for 10.58 % there is a lack of data, 26.46% has been classified in the category “Least concern”, 2.65 % are “Near threatened” and 38. 10% are “Not evaluated”

Mitochondrial DNA Suggests a Western Eurasian origin for Ancient (Proto-) Bulgarians

Ancient (proto-) Bulgarians have long been thought to as a Turkic population. However, evidence found in the past three decades show that this is not the case. Until now, this evidence does not include ancient mitochondrial DNA (mtDNA) analysis. In order to fill this void, we have collected human remains from the VIII-X century AD located in three necropolises in Bulgaria: Nojarevo (Silistra region) and Monastery of Mostich (Shumen region), both in Northeast Bulgaria and Tuhovishte (Satovcha region) in Southwest Bulgaria. The phylogenetic analysis of 13 ancient DNA samples (extracted from teeth) identified 12 independent haplotypes, which we further classified into mtDNA haplogroups found in present-day European and Western Eurasian populations. Our results suggest a Western Eurasian matrilineal origin for proto-Bulgarians as well as a genetic similarity between proto- and modern Bulgarians. Our future work will provide additional data which will further clarify proto-Bulgarian origins; thereby adding new clues to current understanding of European genetic evolution

Detection of Fetal Defects in First Trimester by Ultrasound Examination - Abilities and Limitations

The development of prenatal diagnostics in the recent years and the introduction of the new cell free DNA testing for chromosomal abnormalities raised the question about the effectiveness of the well-known First trimester screening. The need to reassess and to determine the efficacy of the 11-14 week scans in detecting fetuses with chromosomal abnormalities and structural defects arose again. Could the First trimester screening be abandoned and replaced by the new tests? In our practice we find that at 11-14 weeks some abnormalities are always detectable, some can never be and others are potentially detectable depending on their association with increased Nuchal translucency (NT). Fetal structural abnormalities can be classified as major or minor and of early or late onset. After the introduction of a national screening program the prenatal detection rates for all congenital anomalies has increased considerably. Especially anencephaly, gastroschisis and exomphalos are amenable for early detection (in the first trimester). The aim of this study was to determine the efficacy of 11-14 week scan in detecting fetuses with structural anomalies that are almost always detectable in the recent years

Field-induced criticality in a gapped quantum magnet with bond disorder

Neutron diffraction and calorimetric measurements are used to study the field-induced quantum phase transition in piperazinium-Cu$_2$(Cl$_{1-x}$Br$_x$)$_6$ ($x=0$, x=3.5% and x=7.5%), a prototypical quantum antiferromagnet with random bonds. The critical indexes $\phi$ and $\beta$ are determined. The findings contradict some original predictions for Bose Glass based on the assumption $z=d$, but are consistent with recent theoretical results implying $z<d$. Inelastic neutron experiments reveal that disorder has a profound effect on the lowest-energy magnetic gap excitation in the system.Comment: 4.2 pages, 4 figures. Submitted to PRB Rapid Communication

Microvillar and ciliary defects in zebrafish lacking an actin-binding bioactive peptide amidating enzyme

© The Author(s), 2018. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Scientific Reports 8 (2018): 4547, doi:10.1038/s41598-018-22732-9.The assembly of membranous extensions such as microvilli and cilia in polarized cells is a tightly regulated, yet poorly understood, process. Peptidylglycine α-amidating monooxygenase (PAM), a membrane enzyme essential for the synthesis of amidated bioactive peptides, was recently identified in motile and non-motile (primary) cilia and has an essential role in ciliogenesis in Chlamydomonas, Schmidtea and mouse. In mammalian cells, changes in PAM levels alter secretion and organization of the actin cytoskeleton. Here we show that lack of Pam in zebrafish recapitulates the lethal edematous phenotype observed in Pam−/− mice and reveals additional defects. The pam−/− zebrafish embryos display an initial striking loss of microvilli and subsequently impaired ciliogenesis in the pronephros. In multiciliated mouse tracheal epithelial cells, vesicular PAM staining colocalizes with apical actin, below the microvilli. In PAM-deficient Chlamydomonas, the actin cytoskeleton is dramatically reorganized, and expression of an actin paralogue is upregulated. Biochemical assays reveal that the cytosolic PAM C-terminal domain interacts directly with filamentous actin but does not alter the rate of actin polymerization or disassembly. Our results point to a critical role for PAM in organizing the actin cytoskeleton during development, which could in turn impact both microvillus formation and ciliogenesis.This study was supported by grants DK032949 (to BAE and REM), DK044464 (to JDG) and GM051293 (to SMK) from the National Institutes of Health

Strains of the Propionibacterium acnes type III lineage are associated with the skin condition progressive macular hypomelanosis

Progressive macular hypomelanosis (PMH) is a common skin disorder that causes hypopigmentation in a variety of skin types. Although the underlying aetiology of this condition is unclear, there is circumstantial evidence that links the skin bacterium Propionibacterium acnes to the condition. We now describe the first detailed population genetic analysis of P. acnes isolates recovered from paired lesional and non-lesional skin of PMH patients. Our results demonstrate a strong statistical association between strains from the type III phylogenetic lineage and PMH lesions (P = 0.0019), but not those representing other phylogroups, including those associated with acne (type IA(1)). We also demonstrate, based on in silico 16S rDNA analysis, that PMH isolates previously recovered from patients in Europe are also consistent with the type III lineage. Using comparative genome analysis, we identified multiple genomic regions that are specific for, or absent from, type III strains compared to other phylogroups. In the former case, these include open reading frames with putative functions in metabolism, transport and transcriptional regulation, as well as predicted proteins of unknown function. Further study of these genomic elements, along with transcriptional and functional analyses, may help to explain why type III strains are associated with PMH

hArtes: Hardware-Software Codesign for Heterogeneous Multicore Platforms

Developing heterogeneous multicore platforms requires choosing the best hardware configuration for mapping the application, and modifying that application so that different parts execute on the most appropriate hardware component. The hArtes toolchain provides the option of automatic or semi-automatic support for this mapping. During test and validation on several computation-intensive applications, hArtes achieved substantial speedups and drastically reduced development times

Untangling competition between epitaxial strain and growth stress through examination of variations in local oxidation

Understanding corrosion mechanisms is of importance for reducing the global cost of corrosion. While the properties of engineering components are considered at a macroscopic scale, corrosion occurs at micro or nano scale and is influenced by local microstructural variations inherent to engineering alloys. However, studying such complex microstructures that involve multiple length scales requires a multitude of advanced experimental procedures. Here, we present a method using correlated electron microscopy techniques over a range of length scales, combined with crystallographic modelling, to provide understanding of the competing mechanisms that control the waterside corrosion of zirconium alloys. We present evidence for a competition between epitaxial strain and growth stress, which depends on the orientation of the substrate leading to local variations in oxide microstructure and thus protectiveness. This leads to the possibility of tailoring substrate crystallographic textures to promote stress driven, well-oriented protective oxides, and so to improving corrosion performance

Identification of SARS-CoV-2-induced pathways reveals drug repurposing strategies.

The global outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) necessitates the rapid development of new therapies against coronavirus disease 2019 (COVID-19) infection. Here, we present the identification of 200 approved drugs, appropriate for repurposing against COVID-19. We constructed a SARS-CoV-2-induced protein network, based on disease signatures defined by COVID-19 multiomics datasets, and cross-examined these pathways against approved drugs. This analysis identified 200 drugs predicted to target SARS-CoV-2-induced pathways, 40 of which are already in COVID-19 clinical trials, testifying to the validity of the approach. Using artificial neural network analysis, we classified these 200 drugs into nine distinct pathways, within two overarching mechanisms of action (MoAs): viral replication (126) and immune response (74). Two drugs (proguanil and sulfasalazine) implicated in viral replication were shown to inhibit replication in cell assays. This unbiased and validated analysis opens new avenues for the rapid repurposing of approved drugs into clinical trials